Recombinant plant dsRNA-binding protein as an effective tool for the isolation of viral replicative form dsRNA and universal detection of RNA viruses     

Kappei Kobayashi  Reiko Tomita  Masaru Sakamoto 《Journal of General Plant Pathology》2009,75(2):87-91

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Cytological studies on artificially induced gynogenesis in the Pacific abalone   总被引：17，自引：0，他引：17  

Qi Li  Makoto Osada  Masaru Kashihara  Ken Hirohashi and  Akihiro Kijima 《Fisheries Science》2000,66(4):701-707

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Scomber japonicus, H. is a better candidate species for juvenile production activities than Scomber scombrus, L.     

Diego Mendiola  Yoh Yamashita  Michiya Matsuyama  Paula Alvarez  & Masaru Tanaka 《Aquaculture Research》2008,39(10):1122-1127

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Ammonia volatilization from Vietnamese acid sulfate paddy soil following application of digested slurry from biogas digester     

Nakamura  Masato  Oritate  Fumiko  Yuyama  Yoshito  Yamaoka  Masaru  Dan  Nguyen Phuoc  Hanh  Dang Vu Bich 《Paddy and Water Environment》2018,16(1):193-198

Paddy and Water Environment - In some areas of Vietnam, digested slurry from biogas digesters is discharged into canals without wastewater treatment. In order to reduce environmental pollution,...  相似文献   

Tissue concentrations of eosinophilia in the bovine oviduct and uterus of different stages of the oestrous cycle     

H Matsuda  K Okuda  T Imori 《Research in veterinary science》1983,34(3):369-370

Numbers of eosinophils in the bovine oviduct and uterus were determined during the oestrous cycle. The eosinophil numbers in the oviduct (ampulla and isthmus) and horn of the uterus during oestrus were significantly higher than during dioestrus. The number of eosinophils in the uterine cervix was lower than in the uterine horn for all stages of the oestrous cycle. In the oviduct, eosinophils accumulated in the lamina propria of the tunica mucosa, in the tunica muscularis and in the connective tissue of the tunica serosa. In the uterus, they were concentrated mainly in the upper parts of the stroma in the endometrium. Degranulation of eosinophils was observed during oestrus when they increased in number in the oviduct and uterus.  相似文献   

Oncolytic reovirus synergizes with chemotherapeutic agents to promote cell death in canine mammary gland tumor     

Masaya Igase  Chung Chew Hwang  Satoshi Kambayashi  Masato Kubo  Matt Coffey  Takako Shimokawa Miyama  Kenji Baba  Masaru Okuda  Shunsuke Noguchi  Takuya Mizuno 《Canadian journal of veterinary research》2016,80(1):21-31

The oncolytic effects of reovirus in various cancers have been proven in many clinical trials in human medicine. Oncolytic virotherapy using reovirus for canine cancers is being developed in our laboratory. The objective of this study was to examine the synergistic anti-cancer effects of a combination of reovirus and low doses of various chemotherapeutic agents on mammary gland tumors (MGTs) in dogs. The first part of this study demonstrated the efficacy of reovirus in canine MGTs in vitro and in vivo. Reovirus alone exerted significant cell death by means of caspase-dependent apoptosis in canine MGT cell lines. A single injection of reovirus impeded growth of canine MGT tumors in xenografted mice, but was insufficient to induce complete tumor regression. The second part of this study highlighted the anti-tumor effects of reovirus in combination with low doses of paclitaxel, carboplatin, gemcitabine, or toceranib. Enhanced synergistic activity was observed in the MGT cell line treated concomitantly with reovirus and in all the chemotherapeutic agents except toceranib. In addition, combining reovirus with paclitaxel or gemcitabine at half dosage of half maximal inhibitory concentration (IC₅₀) enhanced cytotoxicity by activating caspase 3. Our data suggest that the combination of reovirus and low dose chemotherapeutic agents provides an attractive option in canine cancer therapy.  相似文献   

Analysis of microRNA‐203 function in CREB/MITF/RAB27a pathway: comparison between canine and human melanoma cells          下载免费PDF全文

S. Noguchi  M. Kumazaki  T. Mori  K. Baba  M. Okuda  T. Mizuno  Y. Akao 《Veterinary and comparative oncology》2016,14(4):384-394

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Erratum to: Early epipelagic life-history characteristics of the North Pacific armorhead <Emphasis Type="Italic">Pentaceros wheeleri</Emphasis>     

Chisato Murakami  Shiroh Yonezaki  Satoshi Suyama  Masayasu Nakagami  Takehiro Okuda  Masashi Kiyota 《Fisheries Science》2017,83(1):141-141

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Cultivation of mycoplasma from conjunctiva and production of corneal immune response in guinea pigs.     

L H Campbell  H K Okuda 《American journal of veterinary research》1975,36(7):893-897

Mycoplasma canis was the most frequent mycoplasmal isolant obtained from the conjunctival surface of the eyes of dogs. Nine mycoplasmal isolates, 5 were M canis, were recovered from the lower cul-de-sac of 101 dogs. Experimentally induced immune keratitis was produced by sensitizing guinea pigs to 3 antigen preparations of M canis and then administering intracorneal challenge inoculation. The guinea pigs were clinically observed by leukocyte migration-inhibition (LMI) technique and by histopathologic study of their corneas. The corneal reaction of the guinea pigs was not identical to a clinical entity of the dog.  相似文献   

Heavy thinning in hinoki plantations in Shikoku (southwestern Japan) has limited effects on recruitment of seedlings of other tree species     

Mahoko Noguchi  Kazuki Miyamoto  Shiro Okuda  Takeharu Itou  Atsushi Sakai 《Journal of Forest Research》2016,21(3):131-142

Heavy thinning may provide a feasible means to convert single-species coniferous plantations to broadleaved or mixed forests. To assess this possibility, we monitored tree seedling dynamics of other tree species, the understory microenvironment, and seed availability following heavy thinning (50 % by volume) in two hinoki (Chamaecyparis obtusa) plantations in Shikoku, southwestern Japan. Conventional (35 % by volume) and row thinning (50 % by volume) treatments were also applied at one of the sites. Diffuse transmittance in the understory dramatically increased after all thinning treatments then rapidly decreased, except after row thinning. Heavy thinning accelerated recruitment of small seedlings (with stems <30 cm high) at one of the sites. However, the densities of these seedlings declined sharply, and increases in the densities of larger seedlings recorded in subsequent surveys were very small. Furthermore, at the other site, recruitment of small seedlings under heavy thinning was equivalent to that under conventional thinning and lower than that under row thinning. Abundant species in the recruited seedlings were pioneer or subcanopy species found in the soil seed bank. Low seed rain density at both sites appeared to be responsible for the poor recruitment of canopy tree species. Our results suggest that heavy thinning had limited effects on the seedling recruitment of other tree species in these hinoki plantations due to insufficient seed rain and rapid canopy closure.  相似文献