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61.
为评价甲酸对象草(Pennisetum purpureum Schumach.)青贮发酵品质的影响,分别设置添加量为0(对照),2.2,4.4和6.6 mL·kg-1FM处理,在青贮0.5,1,2,3,5,7,14,30 d开窖,测定青贮饲料发酵品质的动态变化。结果表明:对照发酵品质良好,未见丁酸生成;与对照相比,添加甲酸能够快速酸化青贮饲料,明显提高水溶性碳水化合物含量(P<0.05),且添加量越高效果越好;添加2.2 mL·kg-1甲酸组氨态氮/总氮值在2 d后快速升高,且在3 d后显著高于对照(P<0.05),并从第7 d开始检测到丁酸;添加4.4和6.6 mL·kg-1甲酸组整个青贮过程中无乳酸和挥发性脂肪酸产生,氨态氮/总氮值维持在很低的水平(<12 g·kg-1TN)。综上所述,添加2.2 mL·kg-1甲酸的处理在青贮后期酸化作用减弱,使象草的发酵品质和营养品质变差,而添加4.4 mL·kg-1以上甲酸能够有效地保存象草的营养物质。  相似文献   
62.
为评价添加酶、乳酸菌制剂和酶+乳酸菌制剂对苇状羊茅与箭筈豌豆(7∶3)混合青贮发酵品质的影响,试验设对照组(C)、酶制剂(E)、乳酸菌制剂(LAB)和酶+乳酸菌制剂(E+LAB)4个处理,3个重复,青贮后第7,24,60天开窖取样,测定青贮饲料发酵品质。结果表明,与对照组相比,添加酶或乳酸菌制剂能有效地改善混合青贮发酵品质,组合添加进一步提高了乳酸含量和降低了pH值。与对照组和单独添加乳酸菌组相比,在整个青贮过程中无论是酶单独添加还是组合添加都显示较高的乙酸和总挥发性脂肪酸含量,最高的水溶性碳水化合物含量和最低的氨态氮/总氮。这说明酶和乳酸菌制剂组合添加对改善青贮发酵品质有叠加效应。综上所述,酶和乳酸菌制剂组合添加能更好的改善苇状羊茅和箭筈豌豆混合青贮的发酵品质。  相似文献   
63.
Lettuce big-vein disease, caused by Mirafiori lettuce big-vein virus and Lettuce big-vein associated virus, is suppressed when the pH of field soil becomes acidic. Therefore, we evaluated the effect of soil pH on the activities of Olpidium virulentus, the vector of the viruses. We found that acidic soil, pH less than 6.0, significantly reduced O. virulentus infection of the root and influenced the detection rate of zoospores released in the surrounding water. We concluded that acidic soil suppresses zoospore release from zoosporangia.  相似文献   
64.
Electrospun atactic polypropylene (PP) fibers are thicker than those obtained from isotactic PP, although the viscosity of molten PPs is almost same. Thus we focused on the effect of tacticity of PP on fiber diameters. The PP samples with various tacticity were prepared by changing the blend ratio of isotactic PP and atactic PP. Melt-electrospinning is performed by using blended samples, and then electrospun fibers were observed by scanning electron microscope to evaluate fiber diameter of obtained fibers. It is clear that the diameter of electrospun PP fibers decreases as high tacticity content of PP increases. This result suggests that tacticity of samples is an important factor to control the electrospun fiber diameter.  相似文献   
65.
 :为评价添加不同水平青稞酒糟对青稞秸秆与高羊茅(4∶6)混合青贮发酵品质的影响,试验设对照组(Control)和3个添加水平(10%,20%和30%)的青稞酒糟处理组,青贮后第7,14和30天后打开青贮窖,测定青贮饲料发酵品质。结果表明,添加青稞酒糟可以抑制青贮早期好氧性微生物的活性,促进乳酸发酵,使pH 值快速下降,青贮饲料快速进入稳定阶段。青贮30d后,与对照组相比酒糟处理显著降低(P<0.05)了青贮饲料的pH 值,提高了乳酸和水溶性碳水化合物含量,降低了(P<0.05)氨态氮/总氮及丙酸和丁酸含量。综合考虑,添加20% 酒糟效果更佳。  相似文献   
66.
Ion-selective field-effect transistors (ISFETs), which are integrated devices for ion-selective electrodes using solid-state microelectronics technology, have attracted much attention for convenient monitoring of acid rain, since conventional analytical procedures are time-consuming. In this paper, some recent results of studies on pH and nitrate checkers made of ISFETs using new sensing materials and their application to samples of acid rain are described. A new pH-sensitive film, TiN was deposited onto the gate of ISFET device using RF plasma-enhanced CVD method. The plasma was generated in a mixture of TiCl4, N2 and H2 gases. The prepared pH-ISFETs showed a linear response and the almost theoretical slope sensitivity of 59 mV per pH change with a rapid response of less than 10 seconds. We have obtained fairly good agreement with the pH values using conventional pH glass electrode. A prototype nitrate-checker was prepared with casting newly designed nitrate-sensing materials, plasticizers and PVC based tetrahydrofuran solution onto the ISFET device. We have obtained good agreement with the values using conventional ion-chromatography at mg L?1 level.  相似文献   
67.
The three‐dimensionally real position and movement of the scapula in the lateral side of the ribcage could not be clarified in the horse, since the body size of the horse is too large to apply the CT scanning and image analysis methods. In this study, therefore, we examined the position and the movability of scapula using a carcass of the Falabella which is one of the smallest breeds of the horse. The whole skeletal system in thoracic part of the Falabella could be three‐dimensionally observed by CT scanning method. The three‐dimensional images show that the scapula cranially slides and the ventral part of the scapula dorso‐cranially rotates, when the shoulder joint moves to the most cranial position as simulation. The three‐dimensional rotation angle was approximately 10 degrees. As a result of comparative osteology of the scapula between Falabella and the large draft horse, the infraspinous fossa was caudally enlarged in the larger draft horse, whereas the Falabella had narrower infraspinous fossa. We suggest that it may be due to the adaptational morphological change in the scapula bearing various body weights among breeds. The three‐dimensional CT image analysis and the simulation in carcass contribute to the analysis of the bone movements of the horse during walking and running locomotion as well as the motion analysis from external functional‐morphological data.  相似文献   
68.
Oocyte-specific linker histone H1foo is localized in the oocyte nucleus, either diffusely or bound to chromatin, during the processes of meiotic maturation and fertilization. This expression pattern suggests that H1foo plays a key role in the control of gene expression and chromatin modification during oogenesis and early embryogenesis. To reveal the function of H1foo, we microinjected antisense morpholino oligonucleotides (MO) against H1foo into mouse germinal-vesicle stage oocytes. The rate of in vitro maturation of the antisense MO group was significantly lower than that of the control group. Eggs that failed to extrude a first polar body following injection of antisense MO arrested at metaphase I. Additionally, co-injection of in vitro synthesized H1foo mRNA along with antisense MO successfully rescued expression of H1foo and improved the in vitro maturation rate. There was no difference in the rate of parthenogenesis between the antisense MO and control groups. These results indicate that H1foo is essential for maturation of germinal vesicle-stage oocytes.  相似文献   
69.
The mode of action of soluble silicon against strawberry powdery mildew (Sphaerotheca aphanis var. aphanis) was investigated in four experiments. First, silicon-treated leaves from plants grown with silicate (Si+) and control leaves were excised, inoculated with conidia, and subsequent germination and formation of appressoria in a petri dish was assessed after 24 h. The germination rate was 49.7% on Si+ leaves, and was 67.2% on control leaves (t-test, P < 0.01). Second, we soaked cellulose membranes in various solvents and then placed the membranes on 4% water agar, dusted the membranes with conidia, and examined after 12 h. No difference was apparent between any treatment and the control (distilled water). Third, strawberries growing hydroponically with additional silicon in the medium were inoculated with conidia, and leaves were observed with a scanning electron microscope 1–2 days after inoculation. Germ tubes and secondary hyphae were shorter and had fewer branches on Si+ leaves than on the control. Moreover, penetration appeared to be inhibited. Fourth, the cuticle was separated from leaves from plants grown as in the third experiment, placed on water agar, and dusted with conidia. Germination of conidia, observed with a light microscope, on Si+ leaves was suppressed markedly to 40%–60% of that of the control. These results suggested that soluble silicon induced physiological changes in the cuticle layer after absorption by the plant. In addition, soluble silicate reduced germination of conidia, formation of appressoria, and possibly the penetration of powdery mildew.  相似文献   
70.
The antibody response in horses inoculated with 2 doses of a live equine herpesvirus type 1 vaccine with different vaccination intervals (1 to 3 months) was evaluated with regard to the persistence of virus-neutralizing (VN) antibodies. The durations for which the geometric mean VN titers were maintained significantly higher than those before the first vaccination (P<0.05) were up to 5 months in horses that received the vaccination with a 1-month interval (n=17) and 7 months for those that received it with a 2-month (n=17) or 3-month interval (n=14 or 17). The vaccination program with the 2-month interval was the most effective in maintaining VN antibodies for a long duration with the smallest gap of antibody decline between the first and second vaccinations.  相似文献   
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