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Masahito SUGIMOTO Masayo KANAMOTO Takuya CHIBA Hisashi HIDARI Katsuya KIDA Waka SAITO Motoki OOI Yukinobu SATO Toshiro SAITO 《Animal Science Journal》2008,79(4):443-452
Six Japanese Black (Wagyu) steers (average initial weight 467 ± 45 kg) fitted with a ruminal cannula were used in a split‐plot design experiment comprising a 3 × 3 Latin square design (whole‐plot) and a randomized block design (subplot). The whole‐plot treatments were three different feeding levels of urea‐treated potato pulp (PP) silage‐based concentrate: 1.00%, 1.75% and 2.50% of body weight (BW) (on a dry matter (DM) basis). The subplot treatments consisted of the concentrate formulated to contain either soybean meal (SBM) as a rapidly rumen‐degraded protein source or corn gluten meal (CGM) as a slowly degraded protein source. Dry matter intake tended to be lower (P = 0.071) for CGM (8.9 kg/day) than for SBM (9.4 kg/day). Protein sources had no significant effect on digestibility and in situ degradation. Ruminal ammonia nitrogen (NH3‐N) was lower (P = 0.033) for CGM (7.5 mg/dL) than for SBM (9.5 mg/dL). Protein sources did not affect ruminal pH and the total volatile fatty acids (VFA) concentrations. The molar proportions of ruminal acetate and valerate were higher (P = 0.032) for CGM than for SBM. The maximum daily intake of the PP silage‐based concentrate expressed as a percentage of BW was approximately 1.4% of BW. Dry matter intake was higher (P = 0.046) for steers fed at 1.0% of BW of the PP silage‐based concentrate than for steers fed at 1.75% or 2.5% of BW of the concentrate. The feeding levels of the PP silage‐based concentrate had no effect on DM and nutrients digestibility, except for crude protein (CP) digestibility. CP digestibility tended to be lower (P = 0.071) for steers fed at 1.75% of BW of the PP silage‐based concentrate than for steers fed at 1.0% or 2.5% of BW of the concentrate. The feeding levels of the PP silage‐based concentrate also did not affect the in situ degradation parameter of hay and PP silage. The feeding levels of the PP silage‐based concentrate did not affect ruminal pH, NH3‐N and total VFA concentrations. The molar proportion of acetate was highest for steers fed at 1.0% of BW of the concentrate. In conclusion, in the urea‐treated PP silage‐based concentrate, CGM seems to be more effective than SBM for stabilizing the ruminal NH3‐N concentration and to be advantageous for fiber digestion in the rumen. The feeding levels of the PP silage‐based concentrate did not change the amount of VFA production in the rumen and the DM digestibility. 相似文献
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Shiga A Ota Y Ueda Y Hosoi M Miyajima R Hasegawa K Mizuhashi F 《Journal of toxicologic pathology》2010,23(4):253-260
Focal granulomatous inflammation developed in the livers of five 10-week-old male Sprague-Dawley rats. The characteristic features of this lesion were the presence of foreign body multinucleated giant cells engulfing calcium deposits and site-specific development in a fissure formed in a sub-lobation in the left lobe or interlobar fissure of the medial lobe of the liver. To clarify the pathogenesis of this lesion, rat livers showing abnormal sub-lobation or lobar atrophy, rat livers in an acute dermal toxicity study and guinea pig livers in a skin sensitization test were also examined histologically. Consequently, the present lesion was considered to be a reactive change against calcium that was dystrophically deposited in the area of hepatocellular necrosis due to delayed circulatory disturbance caused by external pressure or extension force. Granulomatous lesions like in the present cases should be differentiated from those caused by evident exogenous pathogens such as chemicals or microorganisms. 相似文献
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Saito K Tagawa M Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(5):637-639
This paper deals with 16 cases presented from April to December 2001 and diagnosed clinically as rabbit syphilis, because they showed distinct lesions around the nose and/or mouth, responded to chemotherapy, and the "Rapid Plasma Reagin" test was positive. Twelve cases exhibited initial symptoms and four were relapses. Lesions around the genitalia and/or anus as well as the nose and/or mouth were seen in 8 cases, and 6 cases indicated sneezing. Fifteen cases were successfully treated with oral administration of chloramphenicol, and one was treated with long-acting penicillin by intramuscular injection. The mean age of onset was 8.8 months. As none of these cases had any mating history, the disease was likely to be maternally transmitted. 相似文献
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Mo CHEN Li-Yi CAI Naoko KANNO Takako KATO Jinxing LU Fan JIN Honghua WANG Masayo SEKITA Masashi HIGUCHI Saishu YOSHIDA Hideji YAKO Hiroki UEHARU Shun-Ichiro IZUMI Yukio KATO 《The Journal of reproduction and development》2013,59(5):457-462
Recently we demonstrated an ectopic expression of the human herpesvirus 1 thymidine
kinase (HHV1-TK) gene by functioning of an intrinsic endogenous promoter in the
transgenic rat (TG-rat), suggesting that HHV1 infection in humans induces expression
of the TK gene with the ectopic promoter in the testis and results in accumulation of
HHV1-TK protein, triggering male infertility similar to that in the TG-rat. Hence, in
this study, we started to investigate a relationship between infection of herpesvirus
and human male infertility. Semen was donated by Chinese male infertile patients (153
men, aged 21–49 years) with informed consent, followed by DNA preparation and
analysis by PCR and DNA sequencing. Semen volume, sperm number and density, and sperm
motility were examined. DNAs of HHV1, HHV4, HHV5 and HHV6 were confirmed by PCR,
electrophoresis and DNA sequencing. Finally, virus DNA was identified in 59 patients
(39%). The number of carriers was 39 (25%) for HHV1, 6 (4%) for HHV4, 33 (22%) for
HHV5 and 3 (2%) for HHV6, respectively. Moreover, double-infection was found in 22
out of 59 specimens (37%), most of which were double-infection of HHV1 and HHV5 (15
out of 22 carriers). Though slight severity was present in some of the carriers, the
relationship between virus infection and sperm impairment was not conclusive.
Accordingly, it is essential to examine whether the viral HHV1-TK gene is expressed
in the testis of the infertile human HHV carrier. 相似文献
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