Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution     

Yasuyuki Onodera  Takumi Arakawa  Rika Yui-Kurino  Masayuki P. Yamamoto  Kazuyoshi Kitazaki  Shigehiko Ebe  Muneyuki Matsunaga  Kazunori Taguchi  Yosuke Kuroda  Shiko Yamashita  Tomoyuki Sakai  Toshiro Kinoshita  Tetsuo Mikami  Tomohiko Kubo 《Euphytica》2015,206(2):365-379

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Uniquely stable 40 kDa subfragment-2 in carp myosin     

Takahashi TT  Takahashi M  Konno K 《Journal of agricultural and food chemistry》2005,53(6):2242-2247

Digestion of carp myofibrils at 30 degrees C in 0.5 M KCl medium with calcium ion generated unique 135 kDa heavy meromyosin (HMM). The HMM was not produced when digested at 10 degrees C. A further digestion of the 135 kD HMM isolated in the absence of calcium ion generated uniquely short subfragment-2 (S-2) with a size of 40 kDa (40 kDa S-2) together with subfragment-1 (S-1). The 40 kDa S-2 was identified by N- and C-end sequencing, and demonstrated to locate the amino end of the rod portion. The unfolding temperature for the 40 kDa S-2 was around 52 degrees C as studied by circular dichroism measurement. The same unfolding peak was also detected with the intact rod together with a large unfolding peak at around 36 degrees C coming from the rest of the rod portion, light meromyosin. The unfolding peak for the 40 kDa S-2 in myosin was a little lower (48 degrees C) than that in free form, suggesting the involvement of the head portion in the stability of the 40 kDa S-2 in the structure.  相似文献   

Displacement above the hypocenter of the 2011 Tohoku-Oki earthquake   总被引：3，自引：0，他引：3  

Sato M  Ishikawa T  Ujihara N  Yoshida S  Fujita M  Mochizuki M  Asada A 《Science (New York, N.Y.)》2011,332(6036):1395

The moment magnitude (M(w)) = 9.0 2011 Tohoku-Oki mega-thrust earthquake occurred off the coast of northeastern Japan. Combining Global Positioning System (GPS) and acoustic data, we detected very large sea-floor movements associated with this event directly above the focal region. An area with more than 20 meters of horizontal displacement, that is, four times larger than those detected on land, stretches several tens of kilometers long along the trench; the largest amount reaches about 24 meters toward east-southeast just above the hypocenter. Furthermore, nearly 3 meters of vertical uplift occurred, contrary to observed terrestrial subsidence.  相似文献   

Use of CD134 as a primary receptor by the feline immunodeficiency virus     

Shimojima M  Miyazawa T  Ikeda Y  McMonagle EL  Haining H  Akashi H  Takeuchi Y  Hosie MJ  Willett BJ 《Science (New York, N.Y.)》2004,303(5661):1192-1195

Feline immunodeficiency virus (FIV) induces a disease similar to acquired immunodeficiency syndrome (AIDS) in cats, yet in contrast to human immunodeficiency virus (HIV), CD4 is not the viral receptor. We identified a primary receptor for FIV as CD134 (OX40), a T cell activation antigen and costimulatory molecule. CD134 expression promotes viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Infection is CXCR4-dependent, analogous to infection with X4 strains of HIV. Thus, despite the evolutionary divergence of the feline and human lentiviruses, both viruses use receptors that target the virus to a subset of cells that are pivotal to the acquired immune response.  相似文献   

Oxygen isotopic compositions of asteroidal materials returned from Itokawa by the Hayabusa mission     

Yurimoto H  Abe K  Abe M  Ebihara M  Fujimura A  Hashiguchi M  Hashizume K  Ireland TR  Itoh S  Katayama J  Kato C  Kawaguchi J  Kawasaki N  Kitajima F  Kobayashi S  Meike T  Mukai T  Nagao K  Nakamura T  Naraoka H  Noguchi T  Okazaki R  Park C  Sakamoto N  Seto Y  Takei M  Tsuchiyama A  Uesugi M  Wakaki S  Yada T  Yamamoto K  Yoshikawa M  Zolensky ME 《Science (New York, N.Y.)》2011,333(6046):1116-1119

Meteorite studies suggest that each solar system object has a unique oxygen isotopic composition. Chondrites, the most primitive of meteorites, have been believed to be derived from asteroids, but oxygen isotopic compositions of asteroids themselves have not been established. We measured, using secondary ion mass spectrometry, oxygen isotopic compositions of rock particles from asteroid 25143 Itokawa returned by the Hayabusa spacecraft. Compositions of the particles are depleted in (16)O relative to terrestrial materials and indicate that Itokawa, an S-type asteroid, is one of the sources of the LL or L group of equilibrated ordinary chondrites. This is a direct oxygen-isotope link between chondrites and their parent asteroid.  相似文献   

The addition of calcium ion chelator,EGTA to thawing solution improves fertilizing ability in frozen–thawed boar sperm     

Tetsuji OKAZAKI  Shuji YOSHIDA  Hisanori TESHIMA  Masayuki SHIMADA 《Animal Science Journal》2011,82(3):412-419

In our previous study, seminal plasma effectively suppressed the induction of sperm to capacitation‐like status and acrosome loss during the thawing process. However, because boar seminal plasma is contaminated with various kinds of bacteria and/or viruses, it is necessary to develop a thawing solution without animal‐derived materials. In this study, we focused on the increase of intracellular Ca²⁺ ([Ca²⁺]_i) in sperm after thawing and the negative effects of sperm qualities. After thawing, the fluorescent intensity of [Ca²⁺]_i indicator, Fluo‐3/AM, and the level of phosphorylated tyrosine residue of protein were increased in the sperm. Next, we investigated whether the addition of Ca²⁺ chelators (ethylenediaminetetraacetic acid (EDTA) and ethyleneglycoltetraacetic acid (EGTA)) improved post‐thawed sperm motility. When the frozen–thawed sperm were treated with 6 mmol/L EDTA + 6 mmol/L EGTA, sperm motility was significantly increased as compared with control (6 mmol/L EDTA alone) at all incubation periods (P < 0.05). The combinational treatment significantly suppressed the elevation of [Ca²⁺]_i and the tyrosine phosphorylation, which improved the acrosomal status and fertilizing ability in vitro. Furthermore, when the thawing method was applied for artificial insemination, the fertilization rate was significantly higher than control (P < 0.05, 33% vs. 82%). Thus, we conclude that the addition of EDTA + EGTA to thawing solution is a beneficial tool for artificial insemination using frozen–thawed boar sperm.  相似文献   

Snowcover Components in Northern Japan     

Izumi Noguchi  Takunori Katoh  Shigekatsu Sakai  Riki Iwata  Masayuki Akiyama  Hideyuki Ohtsuka  Kouichi Sakata  Hirohide Aga  Yutaka Matsumoto 《Water, air, and soil pollution》2001,130(1-4):421-426

Snowcover surveys were carried out in Hokkaido, northern Japan. Snowpack samples were collected using a steel pipe at 80 sites in 1988, 69 sites in 1992, and 66 sites in 1996 and 2000 during the time when the water equivalents of the snowcover are the greatest. Spatial distribution maps of water equivalents and of the concentrations and the accumulated amounts of hydrogen, non-sea-salt sulfate and non-sea-salt calcium ion of the snowcover drawn by Kriging method are discussed. The distribution maps show that water equivalents and the accumulated amounts of hydrogen and non-sea-salt sulfate in all the sample years were largest in the Japan Sea region, as were the ion concentrations of hydrogen and non-sea-salt sulfate. It was estimated this was caused by air pollutants transported from a great distance. The ratio of areas below pH 5.0 increased temporally from 1988 (20%) to 1992, 1996 and 2000 (66–80%). This trend was estimated to be affected by a decline of non-sea-salt calcium concentrations derived from alkaline road dust. In addition, the effect of snowcover components was assessed by comparison between the acid loads of the snowcover and the acid neutralizing capacity of lake water. We found that lakes in the middle of the Japan Sea coast have the potential to be acidified by snowmelt.  相似文献   

Paraticulate metals in waters of a typical irrigation river: The River Sakuragawa,Japan     

Humitake Seki  Masayuki Takahashi  Katsumi Kobayashi  Shozo Ishizaka 《Water, air, and soil pollution》1979,12(2):265-271

The particulate metallic elements in waters of a water system of the River Sakuragawa and Lake Kasumigaura were measured by the multielement analyses using particle-induced X-ray emission. The heavy metal particulates for environmental standards were less than 0.0001 ppm for Cd, 0.00037 ppm for Cr, and 0.00078 ppm both for Hg and Pb in the river waters. The predominant metal particles in the waters were Fe, Mn, and Zn.  相似文献   

Relationship between antibacterial activity of (+)-catechin derivatives and their interaction with a model membrane     

Kajiya K  Hojo H  Suzuki M  Nanjo F  Kumazawa S  Nakayama T 《Journal of agricultural and food chemistry》2004,52(6):1514-1519

(+)-Catechin derivatives with different alkyl chain lengths were synthesized from (+)-catechin and various straight chain alkylaldehydes in the presence of methyl mercaptan, and their antibacterial activities against Gram-positive bacteria were evaluated. The antibacterial activity increased markedly with elongation of the alkyl chain lengths of the derivatives and reached a maximum at a chain of four to seven carbons. Subsequently, interaction of the (+)-catechin derivatives with a model membrane using liposome was investigated. The derivatives with a chain of three carbons or more were found to have very strong affinity for the membrane. The injury action of the derivatives against the membrane was examined with liposome in which calcein was enclosed as a fluorescent indicator. The leakage was observed in the derivatives with chain lengths of four carbons or more. Particularly the derivatives with chains longer than five carbons are considered to destroy the liposome membrane judging from the degree of the fluorescent leakage. These results implied that the lipophilicity and disrupting ability of the (+)-catechin derivatives to the liposome membrane participate in their antibacterial activity.  相似文献   

The status of atmospheric concentrations of ammonia in an intensive dairy farming area in central Japan     

Masayuki HOJITO  Kentaro HAYASHI  Kentaro MURANO  Akinori MORI 《Soil Science and Plant Nutrition》2006,52(2):253-253

(pp. 41–46)
Silicon availability in 36 commercial nursery bed soils was evaluated by four methods the phosphate buffer (pH 6.2, 40 mmol L⁻¹), incubation, supernatant and acetate buffer (pH 4.0, 1 mol L⁻¹) Methods. The influence of silicon availability in the nursery bed soils on the silicon uptake of rice Oryza sativa L. cv. Hitomebore seedlings and the effect of silicon fertilizer application were examined in a glass house in 2002.
The results revealed that the best correlation between silicon content in rice seedlings and available silicon in soils was obtained with the phosphate buffer-solution method ( r  = 0.86). More precise evaluation of available silicon was achieved by grouping soils based on these phosphate absorption coefficients (PAC). The correlation coefficients between silicon content in rice seedlings and available silicon in soils were 0.92 and 0.72 for volcanic soils (PAC > 1500) and non volcanic soils (PAC < 1500), respectively.
We concluded that the phosphate buffer method is the most easily adjusted method for estimation of silicon availability in nursery bed soils, and silicon fertilizers should be applied when silicon availability in non-volcanic nursery bed soils goes below 200 mg kg⁻¹, whereas the level is less than 350 mg kg⁻¹ in volcanic soils.  相似文献