Intraspecific variation of physiological and molecular response to cadmium stress in Populus nigra L     

Gaudet M  Pietrini F  Beritognolo I  Iori V  Zacchini M  Massacci A  Mugnozza GS  Sabatti M 《Tree physiology》2011,31(12):1309-1318

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Accumulation and distribution pattern of macro- and microelements and trace elements in Vitis vinifera L. cv. Chardonnay berries     

Bertoldi D  Larcher R  Bertamini M  Otto S  Concheri G  Nicolini G 《Journal of agricultural and food chemistry》2011,59(13):7224-7236

This paper describes the accumulation pattern of 42 mineral elements in Vitis vinifera L. berries during development and ripening and their distribution in berry skin, seeds, and flesh around harvest time. Grape berries were sampled in two different vineyards with alkaline soil and analyzed using a ICP-MS. Although elemental amounts were significantly different in the grapes from the two vineyards, the accumulation pattern and percentage distribution in different parts of the berries were generally quite similar. Ba, Eu, Sr, Ca, Mg, Mn, and Zn accumulate prior to veraison. Al, Ce, Dy, Er, Ga, Gd, Ho, La, Nd, Pr, Sm, Sn, Zr, Th, Tm, U, Y, and Yb accumulate mainly prior to veraison but also during ripening. Ag, As, B, Cd, Cs, Cu, Fe, Ge, Hg, K, Li, Na, P, Rb, Sb, Se, and Tl accumulate progressively during growth and ripening. With regard to distribution, Ba, Ca, Eu, Fe, Mn, P, Sr, and Zn accumulate mainly in the seeds, Al, B, Ga, Sn, and the rare earths analyzed, except for Eu, accumulate mainly in the skin, and Ag, As, Cd, Cs, Cu, Ge, Hg, K, Li, Mg, Na, Rb, Sb, Se, Th, Tl, U, and Zr accumulate mainly in the flesh. A joint representation of the accumulation and distribution patterns for the elements in the berry is also given.  相似文献   

Image-guided interventional procedures in the dog and cat     

Massimo Vignoli  Jimmy H. Saunders 《Veterinary journal (London, England : 1997)》2011,187(3):297-303

Medical imaging is essential for the diagnostic workup of many soft tissue and bone lesions in dogs and cats, but imaging modalities do not always allow the clinician to differentiate inflammatory or infectious conditions from neoplastic disorders. This review describes interventional procedures in dogs and cats for collection of samples for cytological or histopathological examinations under imaging guidance. It describes the indications and procedures for imaging-guided sampling, including ultrasound (US), computed tomography (CT), magnetic resonance imaging and fluoroscopy. US and CT are currently the modalities of choice in interventional imaging.  相似文献   

Soil communities (Acari Oribatida; Hexapoda Collembola) in a clay pigeon shooting range     

Massimo Migliorini  Gaia Pigino  Pietro Paolo Fanciulli  Fabio Bernini 《Pedobiologia》2005,49(1):1-13

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Amaranth as a rich dietary source of β-sitosterol and other phytosterols     

Marcone MF  Kakuda Y  Yada RY 《Plant foods for human nutrition (Dordrecht, Netherlands)》2003,58(3):207-211

The analysis of 4 commonly available amaranth varieties (Amaranthus K343, RRC1011, K433, K432) revealed the presence of all three major phytosterols (beta-sitosterol, campesterol, stigmas-terol) with a total sterol content being several fold higher than those found in other studied plants. Substantial differences in total phytosterol content and beta-sitosterol content were found between the amaranth varieties. The most commonly cultivated amaranth variety in the United States, i.e., Amaranthus K343 was found to possess the highest levels of phytosterols of the varieties tested. The possibility of screening for superior amaranth varieties with various health properties is outlined.  相似文献   

A framework for the integrated assessment and management of dredged materials in Italy: a case study based on the application of Local Sediment Quality Guidelines     

Fulvio Onorati  Cristian Mugnai  Marina Pulcini  Massimo Gabellini 《Journal of Soils and Sediments》2013,13(2):474-487

Purpose

In order to assess sediment quality and to account for the great geochemical heterogeneity of Italian coasts, Local Sediment Quality Guidelines (LSQGs) have to be defined for specific portions of the coastline based on the approach developed by ICRAM–APAT (2007). This paper describes the application of LSQGs to the harbour of Fiumicino (Rome, Italy). The aims were to evaluate the quality of dredging sediments through an integrated chemical–ecotoxicological approach and to define suitable management options.

Materials and methods

Thirty-eight sediment cores, covering the planned dredging depth, were collected in the study area and sliced into 92 sediment samples. Chemical analyses, including inorganic and organic contaminants as well as microbiological parameters, were carried out for all the samples. A bioassay battery composed by the bacterium Vibrio fischeri, the sea urchin Paracentrotus lividus and the alga Dunaliella tertiolecta was applied on one third of the samples. LSQGs were derived from chemical and ecotoxicological data of the harbour area, and were integrated with those from the identified dumping site. The Baseline Chemical Level, corresponding to an absent or unlikely ecotoxicological hazard, and the Limit Chemical Level, corresponding to a probable ecotoxicological hazard, were the LSQGs derived for each chemical.

Results and discussion

A gradient of increasing contamination, moving inland due to urban and agricultural effluents, was observed. Ecotoxicological analyses, similar to microbiological ones, confirmed the poor sediment quality, with the exception of a sample located at the port entrance, where hydrodynamics flush sediment away from the site. Principal component analysis allowed a clear discrimination of three areas, each affected by a different contamination degree and influenced by many sources related to industrial, commercial and/or urban activities. Using LSQGs and the results of bioassays, it was possible to classify the sediment quality of the whole harbour area and to define the most suitable management options. It was found that Fiumicino harbour sediment was not suitable for either beach nourishment or for offshore dumping, with the exception of a negligible amount located at the port entrance. In turn, most sediment seemed to be suitable for disposal in a properly sealed confined disposal facility or for mechanical/chemical treatment.

Conclusions

This case study provided useful insights for implementing the development of LSQGs for more realistic sediment management and will assist in promoting its application to harbour dredging at the local level.  相似文献   

Private sector involvement in the control of contagious bovine pleuropneumonia (CBPP) in the Kazungula district of Zambia benefitted the community and the control strategy     

Geoffrey Munkombwe Muuka  Nadi Songolo  Swithine Kabilika  Paul Fandamu  Domenico Buonavoglia  Massimo Scacchia 《Tropical animal health and production》2013,45(3):699-703

Contagious bovine pleuropneumonia (CBPP) is a disease of economic importance that is widely distributed in sub-Saharan African and contributes significantly to cattle morbidity and mortality. Lack of resources to implement eradication measures has led to the disease becoming endemic in most areas in sub-Saharan Africa where governments have little resources and the majority of the people are poor. Usually, control and eradication of such diseases as CBPP is treated as a public good by governments and to achieve this, governments are usually assisted by nongovernment organisations, bilateral government programmes and international donors. The private sector, which usually is companies that run businesses to make profit, although not very well established in sub-Saharan Africa could play a big role in the eradication of CBPP in the region. This could play a dual role of promoting investment and also eradicate livestock diseases which have proved a menace in the livestock sector. This paper highlights the role played by the private sector in the control of CBPP in Zambia.  相似文献   

Different evolutionary trends of swine H1N2 influenza viruses in Italy compared to European viruses     

Ana Moreno  Elena Gabanelli  Enrica Sozzi  Davide Lelli  Chiara Chiapponi  Massimo Ciccozzi  Gianguglielmo Zehender  Paolo Cordioli 《Veterinary research》2013,44(1):112

European H1N2 swine influenza viruses (EU H1N2SIVs) arose from multiple reassortment events among human H1N1, human H3N2, and avian influenza viruses. We investigated the evolutionary dynamics of 53 Italian H1N2 strains by comparing them with EU H1N2 SIVs. Hemagglutinin (HA) phylogeny revealed Italian strains fell into four groups: Group A and B (41 strains) had a human H1 similar to EU H1N2SIVs, which probably originated in 1986. However Group B (38 strains) formed a subgroup that had a two-amino acid deletion at positions 146/147 in HA. Group C (11 strains) contained an avian H1 that probably originated in 1996, and Group D (1 strain) had an H1 characteristic of the 2009 pandemic strain. Neuraminidase (NA) phylogeny suggested a series of genomic reassortments had occurred. Group A had an N2 that originated from human H3N2 in the late 1970s. Group B had different human N2 that most likely arose from a reassortment with the more recent human H3N2 virus, which probably occurred in 2000. Group C had an avian-like H1 combined with an N2 gene from one of EU H1N2SIVs, EU H3N2SIVs or Human H3N2. Group D was part of the EU H3N2SIVs clade. Although selection pressure for HA and NA was low, several positively selected sites were identified in both proteins, some of which were antigenic, suggesting selection influenced the evolution of SIV. The data highlight different evolutionary trends between European viruses and currently circulating Italian B strains and show the establishment of reassortant strains involving human viruses in Italian pigs.  相似文献   

Fast field cycling NMR relaxometry characterization of biochars obtained from an industrial thermochemical process   总被引：1，自引：0，他引：1  

Claudio De Pasquale  Valentina Marsala  Anne E. Berns  Massimo Valagussa  Alessandro Pozzi  Giuseppe Alonzo  Pellegrino Conte 《Journal of Soils and Sediments》2012,12(8):1211-1221

Purpose

Biochar has unique properties which make it a powerful tool to increase soil fertility and to contribute to the decrease of the amount of atmospheric carbon dioxide through the mechanisms of C sequestration in soils. Chemical and physical biochar characteristics depend upon the technique used for its production and the biomass nature. For this reason, biochar characterization is very important in order to address its use either for agricultural or environmental purposes.

Materials and methods

Three different biochars obtained from an industrial gasification process were selected in order to establish their chemical and physical peculiarities for a possible use in agronomical practices. They were obtained by charring residues from the wine-making industry (marc) and from poplar and conifer forests. Routine analyses such as pH measurements, elemental composition, and ash and metal contents were performed together with the evaluation of the cross-polarization magic angle spinning (CPMAS) ¹³C NMR spectra of all the biochar samples. Finally, relaxometry properties of water-saturated biochars were retrieved in order to obtain information on pore size distribution.

Results and discussion

All the biochars revealed basic pH values due to their large content of alkaline metals. The quality of CPMAS ¹³C NMR spectra, which showed the typical signal pattern for charred systems, was not affected by the presence of paramagnetic centers. Although paramagnetism was negligible for the acquisition of solid state spectra, it was effective in some of the relaxometry experiments. For this reason, no useful information could be retrieved about water dynamics in marc char. Conversely, both relaxograms and nuclear magnetic resonance dispersion profiles of poplar and conifer chars indicated that poplar char is richer in small-sized pores, while larger pores appear to be characteristic for the conifer char.

Conclusions

This study showed the potential of relaxometry in revealing chemical?Cphysical information on industrially produced biochar. This knowledge is of paramount importance to properly direct biochar agronomical uses.  相似文献   

A ω-secalin contained decamer shows a celiac disease prevention activity     

Pasquale De Vita  Donatella B.M. Ficco  Alessandro Luciani  Olimpia Vincentini  Massimo Pettoello-Mantovani  Marco Silano  Luigi Maiuri  Luigi Cattivelli 《Journal of Cereal Science》2012

Celiac disease (CD) is an autoimmune permanent enteropathy that is triggered in susceptible individuals after the ingestion of gluten, a storage protein fraction presents in wheat, rye and barley endosperm. Specific gluten peptides can bind to HLA-DQ2/8 and induce lamina propria CD4⁺ T cell responses causing damage of the small intestine mucosa. Recent studies suggested that beside immunodominant and toxic epitopes, wheat gluten also contains epitopes capable of preventing the mucosal response in vitro. Among them, a decapeptide (QQPQDAVQPF) from wheat was reported to have an antagonist effect on the agglutination of K562(S) cells and celiac T-cell activation, although the corresponding nucleotidic sequence remained unknown. This study was therefore designed to clone the sequence encoding the protein carrying the decapetide with CD protective properties. A ω-secalin gene encoding containing the decapeptide QQPQRPQQPF was isolated. Although the decapeptide was not identical to the one previously described, QQPQRPQQPF showed the same capability to prevent K562(S) cell agglutination and celiac mucosa immune activation induced by toxic gliadins. The ω-secalin gene was found in wheat carrying the wheat–rye chromosomal translocations 1BL.1RS. The identification of this immunomodulatory gliadin sequence, naturally occurring in cultivars of wheat toxic for celiac patients, might offer new therapeutic strategies for CD.  相似文献