Isolation of a viable but non-culturable suppression mutant of Vibrio vulnificus: role of antioxidant enzymes in surviving stationary phase and low temperatures     

Akihisa Abe  Eiji Ohashi  Huifeng Ren  Tetsuhito Hayashi  Hideaki Endo 《Fisheries Science》2006,72(3):656-664

ABSTRACT:   The viable but non-culturable (VBNC) suppression mutant was isolated. Suppression subtractive hybridization was used to identify differentially expressed genes among cDNA of the VBNC suppression mutant and the wild-type strain. RpoS-dependent catalase was highly expressed from the VBNC suppression mutant during oxidative stress in the stationary phase and at low temperatures. In experiments of H₂O₂ sensitivity, the VBNC suppression mutant showed a peroxide-resistant phenotype in the stationary phase and at low temperatures. These results suggest that VBNC suppression mutant cells use catalase for protection against oxidative stress in the stationary phase and at low temperatures.  相似文献   

Conserved role of nanos proteins in germ cell development   总被引：1，自引：0，他引：1  

Tsuda M  Sasaoka Y  Kiso M  Abe K  Haraguchi S  Kobayashi S  Saga Y 《Science (New York, N.Y.)》2003,301(5637):1239-1241

In Drosophila, maternally supplied Nanos functions in the migration of primordial germ cells (PGCs) into the gonad; in mice, zygotic genes are involved instead. We report the cloning and the functional analyses of nanos2 and nanos3 in mice. These genes are differentially expressed in mouse PGCs. nanos2 is predominantly expressed in male germ cells, and the elimination of this gene results in a complete loss of spermatogonia. However, nanos3 is found in migrating PGCs, and the elimination of this factor results in the complete loss of germ cells in both sexes. Hence, although mice and flies differ in their mechanisms for germ cell specification, there seems to be conserved function for nanos proteins among invertebrates and vertebrates.  相似文献   

Effect of food amount and temperature on growth rate and aerobic scope of juvenile chum salmon     

Iino  Yuki  Kitagawa  Takashi  Abe  Takaaki K.  Nagasaka  Tsuyoshi  Shimizu  Yuichi  Ota  Katsuhiko  Kawashima  Takuya  Kawamura  Tomohiko 《Fisheries Science》2022,88(3):397-409

Offshore migration of Pacific salmon Oncorhynchus spp. is partly triggered by increasing body size and high motility in the early stages of life. The survival of juvenile salmon may depend on their growth rate during the first few months in the sea, and this factor partly regulates the dynamics of adult populations. Here, we assessed the effects of water temperature and food availability on the growth of juvenile chum salmon O. keta. In addition, by combining the measurements of metabolic performance for growth and activity (Absolute Aerobic Scope: AAS) with a bioenergetics model, we estimated the energy allocation for different activities in the juveniles. Under high temperatures (14 °C), juveniles reared at low food levels (1% body weight) allocated less than half their energy for growth than those reared at high food levels (4% body weight). These findings suggest that high temperature and low food level constrain the growth of juveniles, providing an insight into the effect of the recent increase in warm and low-nutrient water masses on survival of juveniles and catches of adult chum salmon on the Pacific side of Honshu Island, Japan.

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Regaining habitats from invasive weeds by planting limited-recruitment endemic trees on an oceanic island: successes and failures 11 years later     

Tetsuto Abe  Takaya Yasui  Midori Yokoya  Marcel Knapp 《Journal of Forest Research》2015,20(1):135-142

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Lack of exposed ice inside lunar south pole Shackleton Crater   总被引：2，自引：0，他引：2  

Haruyama J  Ohtake M  Matsunaga T  Morota T  Honda C  Yokota Y  Pieters CM  Hara S  Hioki K  Saiki K  Miyamoto H  Iwasaki A  Abe M  Ogawa Y  Takeda H  Shirao M  Yamaji A  Josset JL 《Science (New York, N.Y.)》2008,322(5903):938-939

The inside of Shackleton Crater at the lunar south pole is permanently shadowed; it has been inferred to hold water-ice deposits. The Terrain Camera (TC), a 10-meter-resolution stereo camera onboard the Selenological and Engineering Explorer (SELENE) spacecraft, succeeded in imaging the inside of the crater, which was faintly lit by sunlight scattered from the upper inner wall near the rim. The estimated temperature of the crater floor, based on the crater shape model derived from the TC data, is less than approximately 90 kelvin, cold enough to hold water-ice. However, at the TC's spatial resolution, the derived albedo indicates that exposed relatively pure water-ice deposits are not on the crater floor. Water-ice may be disseminated and mixed with soil over a small percentage of the area or may not exist at all.  相似文献   

Agronomic performance and combining ability estimates of yellow maize inbred lines under adequate and deficit moisture conditions     

Amegbor  Isaac Kodzo  Abe  Ayodeji  Adjebeng-Danquah  Joseph  Adu  Gloria Baokyewaa  Oteng-Frimpong  Richard 《Journal of Crop Science and Biotechnology》2022,25(2):183-197

Journal of Crop Science and Biotechnology - Breeding for drought tolerance and increased grain yield is vital in mitigating the threat posed by recurrent drought stress on maize production, as well...  相似文献   

Biochemical and genomic analysis of neoculin compared to monocot mannose-binding lectins     

Shimizu-Ibuka A  Nakai Y  Nakamori K  Morita Y  Nakajima K  Kadota K  Watanabe H  Okubo S  Terada T  Asakura T  Misaka T  Abe K 《Journal of agricultural and food chemistry》2008,56(13):5338-5344

Neoculin occurring in an edible tropical fruit is a heterodimeric protein which has both sweetness and a taste-modifying activity that converts sourness to sweetness. Both the primary and the overall tertiary structures of neoculin resemble those of monocot mannose-binding lectins. This study investigated differences in biochemical properties between neoculin and the lectins. Structural comparison between the mannose-binding sites of lectins and the corresponding regions of neoculin showed that there is at least one amino acid substitution at each site in neoculin, suggesting a reason for the lack of its mannose-binding ability. This was consistent with hemagglutination assay data demonstrating that neoculin had no detectable agglutinin activity. DNA microarray analysis indicated that neoculin had no significant influence on gene expression in Caco-2 cell, whereas kidney bean lectin (Phaseolus vulgaris agglutinin) greatly influenced various gene expressions. These data strongly suggest that neoculin has no lectin-like properties, encouraging its practical use in the food industry.  相似文献   

Dietary supplementation with autochthonous Bacillus cereus improves growth performance and survival in tambaqui Colossoma macropomum          下载免费PDF全文

Joel A R Dias  Higo A Abe  Natalino C Sousa  Márcia V S Couto  Carlos A M Cordeiro  Juliana O Meneses  Fernanda S Cunha  Jose Luiz P Mouriño  Mauricio L Martins  Luís A L Barbas  Paulo C F Carneiro  Alexandre N Maria  Rodrigo Y Fujimoto 《Aquaculture Research》2018,49(9):3063-3070

The aim of this study was to evaluate the effect of dietary supplementation with the autochthonous probiotic bacteria Bacillus cereus on growth performance, haematological parameters and survival of tambaqui (Colossoma macropomum). Tambaqui fingerlings (0.94 ± 0.02 g) were fed for 120 days the following diets: basal (without probiotic) and three supplemented diets (4.2 × 10⁴, 3.9 × 10⁶ and 3.3 × 10⁸ CFU/g). Growth performance and haematological parameters were evaluated every 30 days. Thereafter, fish were challenged against Aeromonas hydrophila and survival was evaluated. Probiotic B. cereus improved weight and length gains (p < 0.05), and increased neutrophils and thrombocyte counts (p < 0.05) in tambaqui supplemented with 3.9 × 10⁶ CFU/g diet. Challenged fish fed unsupplemented diet presented the lowest survival rate (33.4%) while fish fed diets supplemented at 4.2 × 10⁴, 3.9 × 10⁶ and 3.3 × 10⁸ CFU/g B. cereus had 88.8%, 80.5% and 80.5% relative per cent survival respectively. These results demonstrated that B. cereus supplemented as probiotics to C. macropomum for 120 days improved physiological and haematological responses, leading to enhanced survival in this fish species.  相似文献   

Association between the planktonic larval and benthic stages of Manila clam <Emphasis Type="Italic">Ruditapes philippinarum</Emphasis> in eastern Hokkaido,Japan     

Natsuki Hasegawa  Hiroya Abe  Toshihiro Onitsuka  Sayaka Ito 《Fisheries Science》2018,84(2):237-249

The association between planktonic larval and benthic stages of Manila clam Ruditapes philippinarum and factors determining successful recruitment were studied in Akkeshi-ko estuary and Akkeshi Bay, Hokkaido, northern Japan. The field study showed that planktonic larvae were localized mainly in the bay after dispersal from the estuary and recruitment to the estuarine fishing grounds was not always successful. A numerical analysis suggested that estuarine circulation transported tracers, used to simulate planktonic larva, from the estuary to the bay but the number of returning tracers varied depending on the river discharge and wind direction. The planktonic larvae were affected by unsuitable conditions for survival in the estuary, such as high turbidity. We conclude that most larvae develop within the bay, where conditions are more saline and less turbid, and that although return to the estuary is essential for recruitment, it is not necessarily related to the abundance of larvae.  相似文献   

Diversity and community structure of wood-inhabiting fungi found in Japanese wooden houses analyzed by the next-generation sequencing     

Sakae Horisawa  Makoto Yoshida  Kiwamu Umezawa  Tomoko Wada  Hisashi Abe  Shuichi Doi  Masahiro Samejima  Ikuo Momohara 《Journal of Wood Science》2017,63(4):369-378

The diversity and community structures of wood-inhabiting fungi in 16 decayed wood samples from ten wooden houses in Japan were analyzed using a next-generation sequencing (NGS) to determine the fungi responsible for wood decay. DNA of fungi in decayed wood was extracted directly, the internal transcribed spacer (ITS) region in ribosomal DNA (rDNA) was amplified by polymerase chain reaction (PCR), and then, sequences of tagged ITS fragments were analyzed by NGS. Results of sequencing indicated that 68 species of ascomycetes, 37 species of basidiomycetes, and one fungus each from Mortierellales and Mucoromycetes were detected. The fungal community structures showed diversity and included various species of ascomycetes. A microscopic examination of cell wall structure in decayed wood samples suggested that some ascomycetes were soft-rot fungi. Heat map analysis indicated that the similarity in the structures of fungal communities was influenced to a greater extent by the wood species of samples than where they were used as a component.  相似文献