Relationships of survival time, productivity and cause of death with telomere lengths of cows produced by somatic cell nuclear transfer     

Konishi K  Yonai M  Kaneyama K  Ito S  Matsuda H  Yoshioka H  Nagai T  Imai K 《The Journal of reproduction and development》2011,57(5):572-578

The reproductive ability, milk-producing capacity, survival time and relationships of these parameters with telomere length were investigated in 4 groups of cows produced by somatic cell nuclear transfer (SCNT). Each group was produced using the same donor cells (6 Holstein (1H), 3 Holstein (2H), 4 Jersey (1J) and 5 Japanese Black (1B) cows). As controls, 47 Holstein cows produced by artificial insemination were used. The SCNT cows were artificially inseminated, and multiple deliveries were performed after successive rounds of breeding and conception. No correlation was observed between the telomere length and survival time in the SCNT cows. Causes of death of SCNT cows included accidents, accident-associated infections, inappropriate management, acute mastitis and hypocalcemia. The lifetime productivity of SCNT cows was superior to those of the controls and cell donor cows. All SCNT beef cows with a relatively light burden of lactation remained alive and showed significantly prolonged survival time compared with the cows in the SCNT dairy breeds. These results suggest that the lifetime productivity of SCNT cows was favorable, and their survival time was more strongly influenced by environmental burdens, such as pregnancy, delivery, lactation and feeding management, than by the telomere length.  相似文献   

Mycobacterium ulcerans infection in an Indian flap-shelled turtle (Lissemys punctata punctata)     

Sakaguchi K  Iima H  Hirayama K  Okamoto M  Matsuda K  Miyasho T  Kasamatsu M  Hasegawa K  Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1217-1220

We report an atypical mycobacterial infection in an Indian flap-shelled turtle, Lissemys punctata punctata, that died in an aquarium in Japan. At necropsy, the turtle showed multiple white nodules on the capsular surface and parenchyma of various organs such as the liver, spleen, intestine, and lung. Histologically, granulomatous inflammation surrounding a central zone of necrosis was observed. Sections stained by the Ziehl-Neelsen method revealed numerous acid-fast bacilli in the cytoplasm of macrophages and in the central area of necrosis. The organisms were identified as a mycobacterial species by PCR and nucleotide sequence analysis and revealed 98-100% homology to M. ulcerans. This is, to our knowledge, the first report of mycobacteriosis due to M. ulcerans in a turtle.  相似文献   

Small nuclear RNAs and small nuclear ribonucleoprotein complexes purified from the feline placenta     

M Matsuda  T Yamada 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(3):553-559

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A case of feline IgA-monoclonal gammopathy associated with Bence Jones proteinuria     

T Yamada  K Shirota  M Matsuda  T Takahashi  M Ogata  Y Nomura  T Suzuki  H Yamamoto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(3):637-641

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Characterization of protective viral glycoproteins for pseudorabies virus infection.     

A Matsuda  N Okada  S Katayama  T Okabe  N Sasaki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(4):737-741

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In vivo characteristics of a transplantable Marek's disease lymphoblastoid cell line, MDCC-MSB1-41C     

H Matsuda  J Okamoto  Y Sekiya  M Yamada  F Uno  M Murata  S Nii 《Avian diseases》1983,27(4):992-1001

A Marek's disease (MD) lymphoblastoid cell line, MDCC-MSB1-41C, was highly transplantable and lethal for chickens. Autopsies showed extensive metastasis in various organs. The transplantabilities of the parent cell line, MDCC-MSB1, and another derivative line, MDCC-MSB1-33C, were transient. MD virus (MDV) could be isolated from the kidneys but not from the peripheral blood leukocytes of chickens inoculated with the MSB1-41C cell line. In addition, anti-MDV antibodies were produced both in chickens inoculated with this cell line and in controls raised with inoculated chickens, but several attempts to isolate MDV from this cell line in vitro failed.  相似文献   

Analysis of chromosome-sized DNA and genome typing of isolated strains ofTaylorella equigenitalis     

M. Matsuda  Y. Asami  T. Miyazawa  T. Samata  Y. Isayama  M. Honda  Y. Ide 《Veterinary research communications》1994,18(2):93-98

Analysis of chromosome-sized DNA and genome typing ofTaylorella equigenitalis NCTC11184, Kentucky 188, and five strains ofT. equigenitalis isolated in Japan were carried out. The three restriction enzymes used,ApaI,NaeI andNotI, cleaved the genomic DNAs of five Japanese strains ofT. equigenitalis into relatively limited numbers of restriction fragments, which were well resolved on crossed-field gel electrophoresis (CFGE). The respective profiles after CFGE of the restriction fragments from all five strains were essentially identical to each other after digestion byApaI,NaeI orNotI. Hence it appears that these strains have a common genome type with respect to these three restriction enzymes. It was also shown that the respective profiles from these strains were essentially different from those ofT. equigenitalis NCTC11184 and those of Kentucky 188 after digestion withApaI,NaeI orNotI.Abbreviations CFGE crossed-field gel electrophoresis - FIGE field inversion gel electrophoresis - PFGE pulsed-field gel electrophoresis  相似文献   

Expression of monocarboxylate transporter 1 (MCT1) in the dog intestine     

Shimoyama Y  Kirat D  Akihara Y  Kawasako K  Komine M  Hirayama K  Matsuda K  Okamoto M  Iwano H  Kato S  Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):599-604

In this study, the expression and distribution of monocarboxyolate transporter 1 (MCT1) along the intestines (duodenum, jejunum, ileum, cecum, colon and rectum) of dogs were investigated at both the mRNA and protein levels. The expression of MCT1 protein and its distribution were confirmed by Western blotting and immunohistochemical staining using the antibody for MCT1. We identified mRNA coding for MCT1 and a 43-kDa band of MCT1 protein in all regions from the duodenum to the rectum. Immunoreactive staining for MCT1 was also observed in epithelial cells throughout the intestines. MCT1 immunoreactivity was greater in the large intestine than in the small intestine. MCT1 protein was predominantly expressed on the basolateral membranes along intestinal epithelial cells, suggesting that MCT1 may play an important role in lactate efflux and transport of short-chain fatty acids (SCFAs) to the bloodstream across the basolateral membranes of the dog intestine.  相似文献   

Community-associated MRSA SCCmec type IVd in Irish equids     

Maeda Y  Millar BC  Loughrey A  Goldsmith CE  Rooney PJ  Moore JE  Rao J  Buckley T  Egan C  Dooley JS  Lowery CJ  Matsuda M 《The Veterinary record》2007,161(1):35-36

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Induction of ovulation with GnRH and PGF(2 alpha) at two different stages during the early postpartum period in dairy cows: ovarian response and changes in hormone concentrations     

Amaya-Montoya C  Matsui M  Kawashima C  Hayashi KG  Matsuda G  Kaneko E  Kida K  Miyamoto A  Miyake Y 《The Journal of reproduction and development》2007,53(4):867-875

The aims of this study were 1) to determine whether dairy cows can be induced to ovulate by the treatment with gonadotropin releasing hormone (GnRH) followed by prostaglandin F(2 alpha) (PGF(2 alpha)) during the early postpartum period and 2) to describe their ovarian and hormonal responses according to ovarian status. Cows were divided in two groups and received 10 microg of buserelin followed by 500 microg of cloprostenol 7 days apart starting from 21 (GnRH21, n=7) or around 37 days postpartum (GnRH37, n=7). The groups were further classified according to presence (-CL) or absence (-NCL) of functional corpora lutea (CL) on the day of GnRH treatment (d 0): GnRH21-NCL (n=4), GnRH21-CL (n=3) and GnRH37-CL (n=7). Ovarian morphology was monitored and the concentrations of P(4), E(2), FSH and insulin-like growth factor 1 (IGF-1) were measured. All cows ovulated after administration of GnRH. The P(4) levels of the GnRH21-NCL group from d 0 to d 5 were lower than those of the GnRH21-CL (P<0.05) and GnRH37-CL groups (P<0.01). In contrast, the E(2) levels of the GnRH21-NCL group within d 2 to d 6 were higher (P<0.05) than those of the other groups. Compared with the GnRH37-CL group, the GnRH21-NCL group had more small follicles on d 2 (P<0.05), d 3 (P<0.01) and d 4 (P<0.01) and more large follicles on d 5 (P<0.05). The induced CL and new ovulatory follicles were larger in the GnRH21-NCL group compared with the GnRH21-CL (P<0.001 and P<0.01) and GnRH37-CL groups (P<0.001 and P<0.05). IGF-1 did not differ among the groups. The GnRH21-NCL group had higher FSH levels than the GnRH21-CL (P<0.01) and GnRH37-CL groups (P<0.001) on d 0. Low P(4) and high FSH levels may suggest higher gonadotropin support on the enhanced ovarian morphology of the GnRH21-NCL group. PGF(2 alpha) treatment induced CL regression and subsequent ovulation in 3/4 (75%), 3/3 (100%) and 7/7 (100%) cows in the GnRH21-NCL, GnRH21-CL and GnRH37-CL groups, respectively. In conclusion, a 7-day GnRH-PGF(2 alpha) synchronization protocol can effectively induce dairy cows to ovulate as early as 21 days postpartum, regardless of ovarian status.  相似文献