Analysis of chromosome-sized DNA and genome typing of isolated strains ofTaylorella equigenitalis     

M. Matsuda  Y. Asami  T. Miyazawa  T. Samata  Y. Isayama  M. Honda  Y. Ide 《Veterinary research communications》1994,18(2):93-98

Analysis of chromosome-sized DNA and genome typing ofTaylorella equigenitalis NCTC11184, Kentucky 188, and five strains ofT. equigenitalis isolated in Japan were carried out. The three restriction enzymes used,ApaI,NaeI andNotI, cleaved the genomic DNAs of five Japanese strains ofT. equigenitalis into relatively limited numbers of restriction fragments, which were well resolved on crossed-field gel electrophoresis (CFGE). The respective profiles after CFGE of the restriction fragments from all five strains were essentially identical to each other after digestion byApaI,NaeI orNotI. Hence it appears that these strains have a common genome type with respect to these three restriction enzymes. It was also shown that the respective profiles from these strains were essentially different from those ofT. equigenitalis NCTC11184 and those of Kentucky 188 after digestion withApaI,NaeI orNotI.Abbreviations CFGE crossed-field gel electrophoresis - FIGE field inversion gel electrophoresis - PFGE pulsed-field gel electrophoresis  相似文献   

Expression of monocarboxylate transporter 1 (MCT1) in the dog intestine     

Shimoyama Y  Kirat D  Akihara Y  Kawasako K  Komine M  Hirayama K  Matsuda K  Okamoto M  Iwano H  Kato S  Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):599-604

In this study, the expression and distribution of monocarboxyolate transporter 1 (MCT1) along the intestines (duodenum, jejunum, ileum, cecum, colon and rectum) of dogs were investigated at both the mRNA and protein levels. The expression of MCT1 protein and its distribution were confirmed by Western blotting and immunohistochemical staining using the antibody for MCT1. We identified mRNA coding for MCT1 and a 43-kDa band of MCT1 protein in all regions from the duodenum to the rectum. Immunoreactive staining for MCT1 was also observed in epithelial cells throughout the intestines. MCT1 immunoreactivity was greater in the large intestine than in the small intestine. MCT1 protein was predominantly expressed on the basolateral membranes along intestinal epithelial cells, suggesting that MCT1 may play an important role in lactate efflux and transport of short-chain fatty acids (SCFAs) to the bloodstream across the basolateral membranes of the dog intestine.  相似文献   

Community-associated MRSA SCCmec type IVd in Irish equids     

Maeda Y  Millar BC  Loughrey A  Goldsmith CE  Rooney PJ  Moore JE  Rao J  Buckley T  Egan C  Dooley JS  Lowery CJ  Matsuda M 《The Veterinary record》2007,161(1):35-36

  相似文献   

Induction of ovulation with GnRH and PGF(2 alpha) at two different stages during the early postpartum period in dairy cows: ovarian response and changes in hormone concentrations     

Amaya-Montoya C  Matsui M  Kawashima C  Hayashi KG  Matsuda G  Kaneko E  Kida K  Miyamoto A  Miyake Y 《The Journal of reproduction and development》2007,53(4):867-875

The aims of this study were 1) to determine whether dairy cows can be induced to ovulate by the treatment with gonadotropin releasing hormone (GnRH) followed by prostaglandin F(2 alpha) (PGF(2 alpha)) during the early postpartum period and 2) to describe their ovarian and hormonal responses according to ovarian status. Cows were divided in two groups and received 10 microg of buserelin followed by 500 microg of cloprostenol 7 days apart starting from 21 (GnRH21, n=7) or around 37 days postpartum (GnRH37, n=7). The groups were further classified according to presence (-CL) or absence (-NCL) of functional corpora lutea (CL) on the day of GnRH treatment (d 0): GnRH21-NCL (n=4), GnRH21-CL (n=3) and GnRH37-CL (n=7). Ovarian morphology was monitored and the concentrations of P(4), E(2), FSH and insulin-like growth factor 1 (IGF-1) were measured. All cows ovulated after administration of GnRH. The P(4) levels of the GnRH21-NCL group from d 0 to d 5 were lower than those of the GnRH21-CL (P<0.05) and GnRH37-CL groups (P<0.01). In contrast, the E(2) levels of the GnRH21-NCL group within d 2 to d 6 were higher (P<0.05) than those of the other groups. Compared with the GnRH37-CL group, the GnRH21-NCL group had more small follicles on d 2 (P<0.05), d 3 (P<0.01) and d 4 (P<0.01) and more large follicles on d 5 (P<0.05). The induced CL and new ovulatory follicles were larger in the GnRH21-NCL group compared with the GnRH21-CL (P<0.001 and P<0.01) and GnRH37-CL groups (P<0.001 and P<0.05). IGF-1 did not differ among the groups. The GnRH21-NCL group had higher FSH levels than the GnRH21-CL (P<0.01) and GnRH37-CL groups (P<0.001) on d 0. Low P(4) and high FSH levels may suggest higher gonadotropin support on the enhanced ovarian morphology of the GnRH21-NCL group. PGF(2 alpha) treatment induced CL regression and subsequent ovulation in 3/4 (75%), 3/3 (100%) and 7/7 (100%) cows in the GnRH21-NCL, GnRH21-CL and GnRH37-CL groups, respectively. In conclusion, a 7-day GnRH-PGF(2 alpha) synchronization protocol can effectively induce dairy cows to ovulate as early as 21 days postpartum, regardless of ovarian status.  相似文献   

Expression of monocarboxylate transporter 1 in oral and ocular canine melanocytic tumors     

Shimoyama Y  Akihara Y  Kirat D  Iwano H  Hirayama K  Kagawa Y  Ohmachi T  Matsuda K  Okamoto M  Kadosawa T  Yokota H  Taniyama H 《Veterinary pathology》2007,44(4):449-457

  相似文献   

Irradiation history of Itokawa regolith material deduced from noble gases in the Hayabusa samples     

Nagao K  Okazaki R  Nakamura T  Miura YN  Osawa T  Bajo K  Matsuda S  Ebihara M  Ireland TR  Kitajima F  Naraoka H  Noguchi T  Tsuchiyama A  Yurimoto H  Zolensky ME  Uesugi M  Shirai K  Abe M  Yada T  Ishibashi Y  Fujimura A  Mukai T  Ueno M  Okada T  Yoshikawa M  Kawaguchi J 《Science (New York, N.Y.)》2011,333(6046):1128-1131

Noble gas isotopes were measured in three rocky grains from asteroid Itokawa to elucidate a history of irradiation from cosmic rays and solar wind on its surface. Large amounts of solar helium (He), neon (Ne), and argon (Ar) trapped in various depths in the grains were observed, which can be explained by multiple implantations of solar wind particles into the grains, combined with preferential He loss caused by frictional wear of space-weathered rims on the grains. Short residence time of less than 8 million years was implied for the grains by an estimate on cosmic-ray-produced (21)Ne. Our results suggest that Itokawa is continuously losing its surface materials into space at a rate of tens of centimeters per million years. The lifetime of Itokawa should be much shorter than the age of our solar system.  相似文献   

Clock mutation affects circadian regulation of circulating blood cells   总被引：1，自引：0，他引：1  

Katsutaka Oishi  Naoki Ohkura  Koji Kadota  Manami Kasamatsu  Kentaro Shibusawa  Juzo Matsuda  Kazuhiko Machida  Shuichi Horie  Norio Ishida 《Journal of circadian rhythms》2006,4(1):13-7

Background  

Although the number of circulating immune cells is subject to high-amplitude circadian rhythms, the underlying mechanisms are not fully understood.  相似文献   

Immunochemical and physical properties of peptic-digested ovomucoid     

T Matsuda  K Watanabe  R Nakamura 《Journal of agricultural and food chemistry》1983,31(5):942-946

  相似文献   

Comparative studies of the changes in enzymatic activities in hardy and less hardy cultivars of winter wheat in late fall and in winter under snow     

Shonosuke Sagisaka  Yusuke Matsuda  Tohru Okuda  Sachio Ozeki 《Soil Science and Plant Nutrition》2013,59(3):543-550

Abstract

The synthetic activity of protein and changes in the activity profiles of enzymes during cold acclimation were studied in leaves, stems, and crowns of a hardy winter wheat cultivar ( Triticum aestivum L. cv. Horoshirikomugi) and a less hardy winter wheat cultivar (Triticum aestivum L. cv. Norin 61). The results showed that one of the genetic differences between the two cuitivars is found in the activities of protein synthesis under low temperatures. The profiles of the changes in enzymatic activities showed that the adjustment of enzymatic activities was a tissue-specific event to accommodate environmental changes. The synthesis of the enzymes involved in a peroxide scavenging system such as hexokinase, glucose-6-phosphate dehydrogenase, dehydroascorbate reductase, ascorbate peroxidase, and so on started at the acclimation stage, while the phosphoglucose isomerase, which appears to be a maintenance-type enzyme in the wintering wheat, showed a small increase in the activity at later stages of acclimation period in their stems of both cuitivars. When the field was covered with snow, the catalase activity in the leaves started to decrease abruptly, while catalase activities in the stems and crowns did not exhibit appreciable changes during the life under snow. The life-cycle dependent changes in enzymatic activities appear to occur in late March or early April under snow.  相似文献   

Isolation of endophytic streptomycetes from above- and belowground organs of Quercus serrata     

W. Thongsandee  Yosuke Matsuda  M. Shimizu  H. Ehara  S. Ito 《Journal of Forest Research》2013,18(2):179-189

The occurrence and localization of endophytic actinomycetes within diverse organs of host plants provide ecological information that can be used to evaluate the significance of their spatial habitats. The aim of this study was to isolate and characterize endophytic actinomycetes in different organs of Quercus serrata. For this purpose, actinomycete isolates were obtained from surface-sterilized tissues of both symptomless seedlings and aged trees of Q. serrata and rhizosphere soil of the sampled seedlings. Thirty-five isolates with the ability to sporulate, including 4 from leaves of the aged trees, 10, 6, and 15 from leaves, stems, and roots of the seedlings, respectively, and 8 soil-derived isolates, were selected and characterized. The 16S rDNA nucleotide sequence analyses revealed that all of them belonged to the genus Streptomyces. According to a neighbor-joining phylogenetic tree constructed based on the results, the isolates of plant origin were divided into three major clades with high bootstrap values of 98 or 99 %, whereas eight soil-derived isolates were located at different positions from those of the endophyte isolates. Moreover, two larger clades were formed, one of which contained isolates derived only from aboveground parts, while the other contained isolates from all of the organs. These results suggest that the endophytic streptomycetes in Q. serrata may differ in their habitat positions (i.e., either above- or belowground parts).  相似文献