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81.
Dong-Jin Kang Yasuyuki Ishii Aya Nishiwaki 《Journal of Crop Science and Biotechnology》2011,14(2):139-142
In this study, the effects of shoot-cutting method on propagation using overwintering napiergrass (Pennisetum purpureum Schum.) buds were examined under soil conditions compared with plant growth regulators (PGRs) that are used as an effective
method for increasing adventitious buds of several gramineous crops. To increase adventitious budding, we cut shoots of napiergrass
at 2 weeks after planting and treated with the following four types of PGR in comparison with shoot-cutting: 4.44 mM BA as
cytokinin, 3.63 mM triazine as anticytokinin, 5.37 mM NAA as auxin, and 2.22 mM TIBA as antiauxin, either by injection or
direct spraying onto the buds. A large number of adventitious buds were observed in BA- and TIBA-injected buds at 4 WAT compared
to shoot-cut or PGR-sprayed buds. However, an extremely low survival rate for tiller-divided plants (nursery plants) derived
from both BA- and TIBA-injected plants was observed compared with non-PGR controls. Similar trends on the survival rate and
relative tiller number in main shoot-cut plants derived from intact buds (without PGR treatment) were also observed in a repeat
trial. The present study demonstrated that the method of shoot-cutting could contribute to increasing propagation efficiency
for nursery plants compared with exogenous PGR application, and would be a practical method for increased nursery propagation
of napiergrass under soil conditions. 相似文献
82.
Luisella Verotta Marco Guerrini N. A. El-Sebakhy Aya M. Asaad Soad M. Toaima Moahamed E. Abou-Sheer Ying-De Luo John M. Pezzuto 《Fitoterapia》2001,72(8)
From Astragalus peregrinus, four cycloartane-type saponins have been isolated and their structures elucidated by spectral means as 20(R),24(S)-epoxy-9β,19-cyclolanostane-3β,6α,16β,25-tetrol 3-O-β-
-glucopyranoside (1), 20(R),24(S)-epoxy-9β,19-cyclolanostane-3β,6α,16β,25-tetrol 3-O-α-
-rhamnopyranosyl-(1→4)-β-
-glucopyranoside (2), 20(R),24(S)-epoxy-9β,19-cyclolanostane-3β,6α,16β,25-tetrol 3-O-α-
-rhamnopyranosyl-(1→2)-β-
-glucopyranoside (3) and 20(R),25-epoxy-9β,19-cyclolanostane-3β,6α,16β,24(S)-tetrol (24-O-acetyl)- 3-O-α-
-rhamnopyranosyl-(1→2)-(6′-O-acetyl)-β-
-glucopyranoside (4). Compounds 2 and 3 showed to stimulate the proliferation of mouse splenocytes and were not significantly cytotoxic. 相似文献
83.
We used unbiased next-generation sequencing (NGS) to detect unknown viruses in cats. Serum or plasma samples were obtained from clinically ill cats with suspected acute viral infections. Nucleic acid was extracted from serum or plasma samples to construct a complementary DNA library for NGS. Comprehensive nucleotide sequencing analyses enabled detection of the genomes of various viruses, including the severe fever with thrombocytopenia syndrome virus, feline immunodeficiency virus, feline morbillivirus, parvovirus, and Torque teno felis virus. Our findings indicate that comprehensive nucleotide analyses of serum or plasma samples can be used to detect infections with unknown viruses in cats. 相似文献
84.
85.
Tsuji M Zamoto A Kawabuchi T Kataoka T Nakajima R Asakawa M Ishihara C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(7):643-646
Six Eurasian red squirrels (Sciurus vulgaris orientis), victims of road traffic found during 2002 and 2004 near the Noppro Forest Park in Ebetsu, Hokkaido, Japan, were examined for the presence of Babesia parasites. Three of the six squirrels exhibited positive signals by nested PCRs targeting both the 18S rRNA and beta-tubulin genes. Three squirrels proved to be infected with a B. microti-like parasite as evidenced by sequencing the amplified DNAs and by the morphology of the intraerythrocytic parasites. Genotypically, however, the parasite appeared to be of a new type, as it was clearly distinguishable from any of the known types that have previously been reported in various wild animals. This is the first report showing molecular evidence for the presence of B. microti-like parasites in Sciuridae. 相似文献
86.
Kouichi Okamura Naoki Sasaki Takuya Kikuchi Aya Murata Inhyung Lee Haruo Yamada Hisashi Inokuma 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(2):157-160
The purpose of the present study was to evaluate the prokinetic effects of mosapride with non-invasive assessment of myoelectrical activity in the small intestine and caecum of healthy horses after jejunocaecostomy. Six horses underwent celiotomy and jejunocaecostomy, and were treated with mosapride (treated group) at 1.5 mg/kg per osos once daily for 5 days after surgery. The other six horses did not receive treatment and were used as controls (non-treated group). The electrointestinography (EIG) maximum amplitude was used to measure intestinal motility. Motility significantly decreased following surgery. In the treated group, the EIG maximum amplitude of the small intestine was significantly higher than in the controls from day 6~31 after treatment. These findings clearly indicate that mosapride could overcome the decline of intestinal motility after jejunocaecostomy in normal horses. 相似文献
87.
Mitani S Ouchi A Watanabe E Kanesaki Y Nagaoka S Mukai K 《Journal of agricultural and food chemistry》2008,56(12):4406-4417
Kinetic study of the aroxyl radical-scavenging action of catechins (epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and epigallocatechin gallate (EGCG)) and related compounds (methyl gallate (MG), 4-methylcatechol (MC), and 5-methoxyresorcinol (MR)) has been performed. The second-order rate constant ( k s) for the reaction of these antioxidants with aroxyl radical has been measured in ethanol and aqueous Triton X-100 micellar solution (5.0 wt %). The k s values decreased in the order of EGCG > EGC > MC > ECG > EC > MG > MR in ethanol, indicating that the reactivity of the OH groups in catechins decreased in the order of pyrogallol B-ring > catechol B-ring > gallate G-ring > resorcinol A-ring. The structure-activity relationship in the free radical-scavenging reaction by catechins has been clarified by the detailed analyses of the pH dependence of k s values. From the results, the p K a values have been determined for catechins. The monoanion form at catechol B- and resorcinol A-rings and dianion form at pyrogallol B- and gallate G-rings show the highest activity for free radical scavenging. It was found that the free radical-scavenging activities of catechins are 3.2-13 times larger than that of vitamin C at pH 7.0. 相似文献
88.
Mitarai A Ouchi A Mukai K Tokunaga A Mukai K Abe K 《Journal of agricultural and food chemistry》2008,56(1):84-91
The reaction rates ( k s) of tocopherol metabolites (alpha-, gamma-, and delta-CEHC) and Trolox with aroxyl radical have been measured in ethanol and micellar solution by a stopped-flow spectrophotometer, and the k s values obtained were compared with those reported for tocopherols (alpha-, beta-, gamma-, and delta-tocopherol, TocH) and tocol. The rate constants ( k s) increased in the order of Tocol < delta-CEHC < delta-TocH < gamma-CEHC < Trolox approximately gamma-TocH approximately beta-TocH < alpha-CEHC < alpha-TocH in ethanol. The antioxidants that have lower oxidation potentials ( E p) showed higher reactivities. The k s values of alpha-, beta-, gamma-, and delta-tocopherol and tocol in micelle remained constant between pH 4 and pH 10 and decreased rapidly at pH 11~12 by increasing pH value. On the other hand, the k s values of alpha-CEHC, gamma-CEHC, and Trolox showed notable pH dependence. As a result of the detailed analysis of the pH dependence of the rate constants ( k s), the structure-activity relationship in the free radical-scavenging action of the tocopherol metabolites and Trolox has been clarified. 相似文献
89.
Tomofumi Mochizuki Itsuki Iwamoto Aya Atarashi Satoshi T. Ohki Hiroyuki Kikukawa 《Journal of General Plant Pathology》2017,83(4):189-196
A rapid diagnostic method for Japanese yam mosaic virus (JYMV) infection in Chinese yam (Dioscorea polystachya) by a print-capture RT-PCR was developed. The cut surface of a rolled leaf of a JYMV-infected Chinese yam was stamped onto a nitrocellulose membrane. The clipped membrane was boiled in eluting solution to elute the RNA for use as the template in RT-nested PCR. Although JYMV RNA was detectable in most infected plants in the first PCR of the print-capture RT-PCR, it was detected from all samples in the nested PCR. The tissue-printed membranes could be preserved for at least 3 months at 4?°C for JYMV detection. 相似文献
90.
Aya Goto Jiro Sonoda Yuki Seki Yoshikazu Taketa Etsuko Ohta Kyoko Nakano Akira Inomata Kazuhiro Hayakawa Toyohiko Aoki Kazuo Tsukidate Satoru Hosokawa 《Journal of toxicologic pathology》2012,25(3):229-232
Maxillary gingivae from male and female Crl:CD(SD) rats at 12, 16, 21, and 34 weeks of
age were examined histologically. The incidence of gingivitis was approximately 40%, with
no age or sex predilection, and was most frequent between the first and second molar.
Lesions were characterized by acute focal neutrophilic infiltration into the gingival
mucosa, occasionally with inflammatory exudate. In severe cases, inflammation extended to
the periodontal ligament with abscess formation, and adjacent alveolar bone
destruction/resorption. The most characteristic finding was the presence of hair shafts
associated with the lesion, which was observed in approximately 80% of the rats with
gingivitis. These findings suggest that molar gingivitis occurs in rats from an early age
and persists thereafter, and that the main cause of gingivitis in rats is hair penetration
into the gingiva. It would be prudent to keep these background lesions in mind as
potential modifiers in toxicity studies. 相似文献