Toxigenic type D Pasteurella multocida and progressive atrophic rhinitis in New South Wales pig herds     

IA GARDNER  GJ EAMENS  MJ TURNER  CL HORNITZKY 《Australian veterinary journal》1991,68(11):364-365

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Diagnosis of equine herpesvirus 1 abortion using polymerase chain reaction     

JT MACKIE  GA MACLEOD  GH REUBEL  MJ STUDDERT 《Australian veterinary journal》1996,74(5):390-391

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The pathology of Johne''s disease in sheep   总被引：4，自引：0，他引：4  

MJ CARRIGAN  JT SEAMAN 《Australian veterinary journal》1990,67(2):47-50

The clinical, gross and histopathological findings in 50 sheep affected with Johne's disease are described. Clinically 90% were emaciated and 20% showed severe diarrhoea. On necropsy there was thickening of the walls of the intestines, particularly of the ileum, caecum and less frequently the jejunum, but in 36% of sheep the changes were only mild. Histologically there was a granulomatous enteritis, typhlitis and colitis, with the most severe changes in the terminal ileum. High numbers of acid-fast organisms were present in the terminal ileum in over 70% of sheep. Mycobacterium paratuberculosis was cultured from only 8% of the sheep examined.  相似文献   

QTL mapping of volatile compounds in ripe apples detected by proton transfer reaction-mass spectrometry     

Elena?Zini  Franco?Biasioli  Flavia?Gasperi  Daniela?Mott  Eugenio?Aprea  Tilmann?D.?M?rk  Andrea?Patocchi  Cesare?Gessler  Matteo?KomjancEmail author 《Euphytica》2005,145(3):269-279

The availability of genetic linkage maps enables the detection and analysis of QTLs contributing to quality traits of the genotype. Proton Transfer Reaction Mass Spectrometry (PTR-MS), a relatively novel spectrometric technique, has been applied to measure the headspace composition of the Volatile Organic Compounds (VOCs) emitted by apple fruit genotypes of the progeny ‘Fiesta’ × ‘Discovery’. Fruit samples were characterised by their PTR-MS spectra normalised to total area. QTL analysis for all PTR-MS peaks was carried out and 10 genomic regions associated with the peaks at m/z = 28, 43, 57, 61, 103, 115 and 145 were identified (LOD > 2.5). We show that it is possible to find quantitative trait loci (QTLs) related to PTR-MS characterisation of the headspace composition of single whole apple fruits indicating the presence of a link between molecular characterisation and PTR-MS data. We provide tentative information on the metabolites related to the detected QTLs based on available chemical information. A relation between apple skin colour and peaks related to carbonyl compounds was established. The two authors contributed equally to this work.  相似文献   

A chemometric approach to the comparison of different sample treatments for metals determination by atomic absorption spectroscopy in aceto Balsamico tradizionale di Modena     

Cocchi M  Franchini G  Manzini D  Manfredini M  Marchetti A  Ulrici A 《Journal of agricultural and food chemistry》2004,52(13):4047-4056

A comparison of different digestion procedures has been carried out for the analysis of metal concentration in samples of vinegars and Aceto Balsamico Tradizionale of Modena (ABTM) coming from an unique barrel set. In particular, classical wet, dry ashing, and closed vessel microwave digestion procedure have been utilized and compared for each investigated species. In a few cases, direct metal determination on ABTM (without treatment procedure) is proposed as possible alternative to sample manipulation. Flame atomic absorption spectrometry was used for the quantification of iron and zinc, while graphite furnace atomic absorption spectrometry was used for all the other elements (i.e., chromium, manganese, cobalt, nickel, copper, cadmium, and lead). The comparison among the different sample treatments was carried out by the use of statistical and chemometric tools. In particular, principal component analysis and ANOVA approaches were used to discriminate between the diverse analytical methods. Furthermore, for all the dissolving techniques, the analytical metal recovery was always evaluated by the application of the recovery function on the same sample matrix. In general, the recoveries were fairly good, ranging from 90 to 103%, except for Cd and Pb with dry ashing, which showed recovery values close to 55% and 67%, respectively. As regards the metals concentration of the investigated samples, the experimental data reveal for some species the presence of concentration slightly over the legal limit fixed for wine and wine vinegar.  相似文献   

Crop and soil response to subsoil loosening, deep incorporation of phosphorus and potassium fertilizer and subsequent soil management on a range of soil types.: Part 2: Soil structural conditions   总被引：2，自引：0，他引：2  

G.C. Soane  R.J. Godwin  MJ. Marks  G. Spoor 《Soil Use and Management》1987,3(3):123-130

Abstract. In a series of experiments on 16 sites both a power-driven rotary-tine Wye Double Digger and a rigid tine winged subsoiler produced significant subsoil loosening and fissuring. The Double Digger consistently produced the greatest clod breakdown together with the least soil bulk densities and cone penetration resistances. Management strategies after loosening had an important influence on the longevity of the loosening effect. The rate of recompaction was least with controlled traffic and bed systems and increased with random traffic and with the growing of root crops. No significant differences in crop response were monitored between the two loosening treatments, yield response depending largely on the extent of moisture stress experienced by the crop. Loosening on silty soils reduced yields in wet seasons and this was associated with soil structural instability. Visual soil profile examination is necessary to support bulk density and cone penetration resistance measurements when assessing soil compaction.  相似文献   

A hierarchical model for the analysis of spatial rainfall extremes     

Carlo Gaetan  Matteo Grigoletto 《Journal of Agricultural, Biological & Environmental Statistics》2007,12(4):434-449

In this article, we propose a spatial model for analyzing extreme rainfall values over the Triveneto region (Italy). We assess the existence of a long-term trend in the extremes. To integrate data coming from the different stations, we propose a hierarchical model. At the first level, for each monitoring station we model data by making use of a generalized extreme value distribution; at the second level, we combine results from the first stage by exploiting recent advances in modeling nonstationary spatial random fields.  相似文献   

Mitotic Golgi partitioning is driven by the membrane-fissioning protein CtBP3/BARS     

Hidalgo Carcedo C  Bonazzi M  Spanò S  Turacchio G  Colanzi A  Luini A  Corda D 《Science (New York, N.Y.)》2004,305(5680):93-96

Organelle inheritance is an essential feature of all eukaryotic cells. As with other organelles, the Golgi complex partitions between daughter cells through the fission of its membranes into numerous tubulovesicular fragments. We found that the protein CtBP3/BARS (BARS) was responsible for driving the fission of Golgi membranes during mitosis in vivo. Moreover, by in vitro analysis, we identified two stages of this Golgi fragmentation process: disassembly of the Golgi stacks into a tubular network, and BARS-dependent fission of these tubules. Finally, this BARS-induced fission of Golgi membranes controlled the G2-to-prophase transition of the cell cycle, and hence cell division.  相似文献   

Laser-induced selectivity for dimerization versus polymerization of butadiene under pressure     

Citroni M  Ceppatelli M  Bini R  Schettino V 《Science (New York, N.Y.)》2002,295(5562):2058-2060

The pressure-induced chemical reaction of liquid butadiene was studied by Fourier transform infrared spectroscopy in a diamond anvil cell. Dimerization was found to occur above 0.7 gigapascal, giving vinylcyclohexene according to a cyclo-addiction reaction and only a trace amount of polybutadiene forms. By irradiating the high-pressure sample with a few milliwatts of the 488-nanometer argon+ laser line, the dimerization was completely inhibited, and the rapid formation of pure trans-polybutadiene was observed. The use of different excitation wavelength allows us to emphasize the selectivity of the process and to identify the active role of the 2(1)Ag state in this pressure- and laser-induced chemical reaction.  相似文献   

G protein signalling involved in host recognition and mycoparasitismrelated chitinase expression in Trichoderma atroviride          下载免费PDF全文

Susanne Zeilinger　 Barbara Reithner　 Kurt Brunner　 Valeria Scala　 Isabel Peiβl　 Matteo Lorito　 Robert L Mach 《浙江大学学报(农业与生命科学版)》2004,30(4):448-448

Mycoparasitic species of Trichoderma are commercially applied as biological control agents against various fungal pathogens. The mycoparasitic interaction is host specific and includes recognition, attack and subsequent penetration and killing of the host. Investigations on the underlying events revealed that Trichoderma responds to multiple signals from the host (e.g. lectins or other ligands such as low molecular weight components released from the host's cell wall) and host attack is ac…  相似文献