A retrospective analysis of the efficacy of Oncept vaccine for the adjunct treatment of canine oral malignant melanoma     

J. M. Ottnod  R. C. Smedley  R. Walshaw  J. G. Hauptman  M. Kiupel  J. E. Obradovich 《Veterinary and comparative oncology》2013,11(3):219-229

Oral malignant melanoma (OMM) in the dog is often locally aggressive with a high metastatic potential and there are few treatment options that have been demonstrated to improve outcome of this disease. The purpose of this study was to determine whether adjunctive treatment with the Oncept melanoma vaccine affected the outcome of dogs with OMM that had achieved loco‐regional cancer control. Medical records from 45 dogs that presented to the Animal Cancer and Imaging Center were reviewed, including 30 dogs with stage II and III disease. Dogs that received the vaccine did not achieve a greater progression‐free survival, disease‐free interval or median survival time than dogs that did not receive the vaccine.  相似文献   

Predicting species-specific basal areas in urban forests using airborne laser scanning and existing stand register data     

Inka Pippuri  Matti Maltamo  Petteri Packalen  Juha Mäkitalo 《European Journal of Forest Research》2013,132(5-6):999-1012

The aim of this work was to examine how well species-specific stand attributes can be predicted using a combination of airborne laser scanning (ALS) and existing stand register data in urban forests. In this context, the ability of three data combinations: ALS data and stand register data, ALS data and digital aerial images and all of these combined, was tested in the prediction of species-specific basal areas. We divided tree species into seven and three different tree species strata and applied two prediction methods: (1) regression method, in which the predicted total basal area was divided into tree species based on tree species proportions from stand register data, and (2) the nearest neighbour (NN) method, in which tree species proportions were used as predictor variables for species-specific basal areas. Prediction models were built based on training data of 205 field plots, and the accuracy of the models was tested based on validation data of 52 forests stands. Our results showed that species-specific predictions of seven tree species were more accurate when tree species proportions from stand register data were used in the prediction. Both the regression and the NN method provided reasonable accuracy. This study showed that tree species information from existing stand register data could be used as an alternative for aerial images in ALS-based forests inventories. The use of ALS data together with stand register data and small field data could also be economically beneficial in an inventory of urban forests.  相似文献   

T cell rich,B cell lymphoma in the small colon of a yearling horse     

K. M. Smith  C. K. Clark  M. J. Dark  M. Kiupel  J. Gary 《Equine Veterinary Education》2013,25(2):74-78

A one‐year‐old, Thoroughbred colt presented for evaluation due to a one month history of fever of unknown origin and progressive weight loss. On initial presentation, the horse was febrile and showed signs localised to the respiratory tract. These included bilaterally increased bronchovesicular sounds and a moderate, diffuse interstitial pattern on thoracic radiographs. A transtracheal wash yielded mucopurulent debris, culture of which grew small numbers of Staphylococcus epidermidis and Aspergillus spp. The horse was discharged with a diagnosis of bronchointerstitial pneumonia and placed on antibiotic therapy. Ten days after initial presentation, he developed abdominal pain that was unresponsive to on‐farm treatment. The horse was febrile, displayed increased respiratory rate and effort, and showed moderate signs of abdominal pain. On rectal examination, a firm, 8–10 cm mass was palpated on midline. The colt was admitted to the hospital and scheduled for exploratory laparotomy, but died a short time later before surgery could be performed. This report describes the clinical, diagnostic and histopathological findings of a case of alimentary lymphoma in a yearling colt.  相似文献   

Rapid detection of tetracyclines and their 4-epimer derivatives from poultry meat with bioluminescent biosensor bacteria     

Virolainen NE  Pikkemaat MG  Elferink JW  Karp MT 《Journal of agricultural and food chemistry》2008,56(23):11065-11070

Tetracycline (TC) specific luminescent bacterial biosensors were used in a rapid TC residue assay sensitized to meet the EU maximum residue limit (MRL) for TC residues in poultry muscle tissue (100 microg kg(-1)) by membrane-permeabilizing and chelating agents polymyxin B and EDTA. Sensitivities of 5 ng g(-1) for doxycycline, 7.5 ng g(-1) for chlortetracycline, and 25 ng g(-1) for tetracycline and oxytetracycline were reached. Except for doxycycline, the MRLs of these tetracyclines include their 4-epimer metabolites. In the biosensor assay, all four 4-epimers showed induction capacity and antimicrobial activity, and antimicrobial activity was also observed in the inhibition assay, although with lower efficiency than that of the corresponding parent compound in both assays. The biosensor assay is an inexpensive and rapid high-throughput screening method for the detection of 4-epimer TC residues along with their parent compounds.  相似文献   

Utilization Potential of Peats – a Study on Peat Biodegradability Determined by Respirometric Method     

Katri Roppola  Toivo Kuokkanen  Kauko Kujala  Matti Kuokkanen 《Water, air, and soil pollution》2008,192(1-4):59-66

The biodegradation of different peat types was studied with a manometric respirometric test. Compaction peat and sphagnum peat samples were analysed, and the effect of peat pH on biodegradation behaviour was evaluated. Only minor (BOD/ThOD?<?0.4%) biodegradation was observed with compaction peat samples, and the stable state, in which biodegradation stopped, was achieved during a two month period. As expected, sphagnum peat samples with a lower decomposition rate degraded more than compaction peat samples. Alkalinity (pH between ca. 4–9) of the peat was noticed to reduce the degree of biodegradation and accelerate the achievement of the stable state.  相似文献   

Physiological characterization of ocular melanosis‐affected canine melanocytes     

Ethan M. Dawson‐Baglien  Erica L. Noland  Dodd G. Sledge  Matti Kiupel  Simon M. Petersen‐Jones 《Veterinary ophthalmology》2019,22(2):132-146

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Listerial mesenteric lymphadenitis in 3 cats     

Thomas W. Fluen  Michael Hardcastle  Matti Kiupel  Randolph M. Baral 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(4):1753-1758

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Ultrastructure of porcine circovirus in persistently infected PK-15 cells.   总被引：7，自引：0，他引：7  

G W Stevenson  M Kiupel  S K Mittal  C L Kanitz 《Veterinary pathology》1999,36(5):368-378

The ultrastructure of porcine circovirus was examined in persistently infected porcine kidney (PK)-15 cells. Virus-infected PK-15 cells had large numbers of intracytoplasmic inclusions, and a few cells had intranuclear inclusions. Intracytoplasmic inclusions were dispersed throughout the cytoplasm but were most numerous in the perinuclear cytoplasm. Inclusion were of various sizes, round to oval, and electron dense and were of two general types. Inclusions of the first type were small (0.1-0.5 microm diameter), not surrounded by trilaminar membranes, and granular with indistinct margins that blended with surrounding cytoplasm. Some contained 12+/-2-nm-diameter icosahedral virions in loose aggregates or rarely forming paracrystalline arrays. Small inclusions could be sites of viral assembly or maturation. Intracytoplasmic inclusions of the second type were larger (0.5-5.0 microm diameter) and more numerous and had abrupt margins surrounded by trilaminar membranes. They were more electron dense than small inclusions and were heterogeneous, containing various proportions of aggregated virions, electron-dense crystalline lamellae of 5 nm periodicity, and/or whorls of myelinoid membranes. Virions usually formed paracrystalline arrays and occasionally were loosely aggregated. Larger inclusions were typical of autophagolysosomes. Intranuclear inclusions were not membrane bound and were often associated with reticulated nucleoli or aggregates of heterochromatin. Some inclusions were irregularly shaped aggregates of indistinct, circular 10-12-nm-diameter viruslike particles. Others were 0.1-1.0 microm in diameter, round or ring shaped, dense, and finely granular, with sharply demarcated margins.  相似文献   

Cost analysis of a satellite terminal for forest fuel supply in Finland     

Matti Virkkunen  Olli-Jussi Korpinen 《Scandinavian Journal of Forest Research》2016,31(2):175-182

As forest fuel demand increases, new logistical solutions are needed. Most of the increase in use is expected to take place in large heat and power production units which set special requirements for the supply as both procurement volumes and transport distances increase. Biomass fuel terminals broaden the spectrum of available supply options by offering cost-effective large-scale biomass storage and processing options for securing the fuel supply in all conditions. This study aimed to study different costs of a satellite terminal and to produce important concept and cost information for developing forest fuel logistics based on future terminals. The figures indicate that terminals do not create direct cost benefits per se: direct supply chains are more economical compared to supply through terminals. However, there are several indirect benefits that can be reached via fuel supply through terminals: regional fuel procurement can be widened to a national scale, security of supply increases through easily available storages, large supply volumes can be delivered by an individual operator, prices remain more stable and a more even quality of delivered fuel can be achieved.  相似文献   

Recurrence rates and clinical outcome for dogs with grade II mast cell tumours with a low AgNOR count and Ki67 index treated with surgery alone          下载免费PDF全文

J. Smith  M. Kiupel  J. Farrelly  R. Cohen  G. Olmsted  J. Kirpensteijn  B. Brocks  G. Post 《Veterinary and comparative oncology》2017,15(1):36-45

Grade II mast cell tumours (MCT) are tumours with variable biologic behaviour. Multiple factors have been associated with outcome, including proliferation markers. The purpose of this study was to determine if extent of surgical excision affects recurrence rate in dogs with grade II MCT with low proliferation activity, determined by Ki67 and argyrophilic nucleolar organising regions (AgNOR). Eighty‐six dogs with cutaneous MCT were evaluated. All dogs had surgical excision of their MCT with a low Ki67 index and combined AgNORxKi67 (Ag67) values. Twenty‐three (27%) dogs developed local or distant recurrence during the median follow‐up time. Of these dogs, six (7%) had local recurrence, one had complete and five had incomplete histologic margins. This difference in recurrence rates between dogs with complete and incomplete histologic margins was not significant. On the basis of this study, ancillary therapy may not be necessary for patients with incompletely excised grade II MCT with low proliferation activity.  相似文献