排序方式： 共有86条查询结果，搜索用时 15 毫秒
71.
Namiko IKEDA Daiki KATO Masaya TSUBOI Ryohei YOSHITAKE Shotaro ETO Sho YOSHIMOTO Masahiro SHINADA Satoshi KAMOTO Yuko HASHIMOTO Yousuke TAKAHASHI James CHAMBERS Kazuyuki UCHIDA Ryohei NISHIMURA Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1885
Cancer immunotherapy is a novel cancer treatment for canine tumors. Indoleamine 2,3-dioxygenase 1 (IDO1) is overexpressed in some human tumors and inhibits antitumor immunity. In this study, we comprehensively evaluated expression pattern of IDO1 and the nature of IDO1-expressing cells in canine normal and tumor tissues. In normal tissue samples, IDO1 expression was detected only in the lymph nodes, spleen, tonsil tissues, and colon tissues. In contrast, IDO1-positive tumor cells were observed in several tumor tissue types. This is the first study to evaluate IDO1 expression in canine normal and tumor tissues, and the results suggest that IDO1 is a promising target for novel cancer immunotherapy in dogs with tumors. 相似文献
72.
Naomi TERAMOTO Masanari IKEDA Hidetoshi SUGIHARA Takanori SHIGA Takashi MATSUWAKI Masugi NISHIHARA Kazuyuki UCHIDA Keitaro YAMANOUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1416
Rhabdomyosarcoma (RMS) is an aggressive type of soft tissue sarcoma, and pleomorphic RMS is a rare subtype of RMS found in adult. p16 is a tumor suppressor which inhibits cell cycle. In human RMS, p16 gene is frequently deleted, but p16-null mice do not develop RMS. We reported that genetic ablation of p16 by the crossbreeding of p16 knock-out rats (p16-KO rats) improved the dystrophic phenotype of a rat model of Duchenne muscular dystrophy (Dmd-KO rats). However, p16/Dmd double knock-out rats (dKO rats) unexpectedly developed sarcoma. In the present study, we raised p16-KO, Dmd-KO, and dKO rats until 11 months of age. Twelve out of 22 dKO rats developed pleomorphic RMS after 9 months of age, while none of p16-KO rats and Dmd-KO rats developed tumor. The neoplasms were connected to skeletal muscle tissue with indistinct borders and characterized by diffuse proliferation of pleomorphic cells which had eosinophilic cytoplasm and atypical nuclei with anisokaryosis. For almost all cases, the tumor cells immunohistochemically expressed myogenic markers including desmin, MyoD, and myogenin. The single cell cloning from tumor primary cells gained 20 individual Pax7-negative MyoD-positive RMS cell clones. Our results demonstrated that double knock-out of p16 and dystrophin in rats leads to the development of pleomorphic RMS, providing an animal model that may be useful to study the developmental mechanism of pleomorphic RMS. 相似文献
73.
Satoshi KAMOTO Masahiro SHINADA Daiki KATO Masaya TSUBOI Sho YOSHIMOTO Ryohei YOSHITAKE Shotaro ETO Namiko IKEDA Yosuke TAKAHASHI Yuko HASHIMOTO James CHAMBERS Kazuyuki UCHIDA Shinji YAMADA Mika K. KANEKO Ryohei NISHIMURA Yukinari KATO Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1795
Podoplanin is expressed in various human tumors where it promotes tumor progression, epithelial-mesenchymal transition, and distant metastasis. Podoplanin is also expressed in cancer-associated fibroblasts and induces tumor malignancy. The objective of this study was to evaluate podoplanin expression in various types of feline tumor tissues. Immunohistochemical analysis revealed that podoplanin was expressed in cells of 13/15 (87%) squamous cell carcinomas and 5/19 (26%) fibrosarcomas. Moreover, cancer-associated fibroblasts expressed podoplanin in most tumor types, including 18/21 (86%) mammary adenocarcinoma tissues. Our findings demonstrate that various types of feline tumor tissues expressed podoplanin, indicating the importance of the comparative aspects of podoplanin expression, which may be used as a novel research model for podoplanin biology. 相似文献
74.
75.
Saki SAKUMA Mariko OKAMOTO Nao MATSUSHITA Masami UKAWA Takumi TOMONO Keiko KAWAMOTO Teruo IKEDA Shinji SAKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):484
Poly(N-vinylacetamide-co-acrylic acid) coupled with d-octaarginine (VP-R8) promotes the cellular uptake of peptides/proteins in vitro; however, details of the transfection efficacy of VP-R8, such as the cell types possessing high gene transfer, are not known. Herein, we compared the ability of VP-R8 to induce the cellular uptake of plasmid DNA in mouse and human cell lines from different tissues and organs. A green fluorescent protein (GFP)-expression plasmid was used as model genetic material, and fluorescence as an indicator of uptake and plasmid-derived protein expression. Three mouse and three human cell lines were incubated with a mixture of plasmid and VP-R8, and fluorescence analysis were performed two days after transfection. To confirm stable transgene expression, we performed drug selection three days after transfection. A commercially available polymer-based DNA transfection reagent (PTR) was used as the transfection control and standard for comparing transgene expression efficiency. In the case of transient transgene expression, slight-to-moderate GFP expression was observed in all cell lines transfected with plasmid via VP-R8; however, transfection efficiency was lower than using the PTR for gene delivery. In the case of stable transgene expression, VP-R8 promoted drug-resistance acquisition more efficiently than the PTR did. Cells that developed drug resistance after VP-R8-mediated gene transfection expressed GFP more efficiently than cells that developed drug resistance after transfection with the PTR. Thus, VP-R8 shows potential as an in vitro or ex vivo nonviral transfection tool for generating cell lines with stable transgene expression. 相似文献
76.
77.
78.
Jun Murase Kumiko Itoh Mayuko Kana Makoto Kimura 《Soil Science and Plant Nutrition》2013,59(6):909-913
The structure of the β-proteobacterial autotrophic ammonia-oxidizing bacterial (AOB) communities in a microcosm of submerged paddy soil was determined by denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments amplified using AOB-selective primers. Shift in the community composition was observed 4 weeks after submergence. The communities from the surface layers (0–1, 2–3 mm) of the soil microcosm were different from those of the subsurface layers (6–9, > 15 mm) and DGGE bands specific to each layer were detected. The majority of the retrieved sequences were Nitrosospira-like, whereas no Nitrosomonas-like sequences were obtained. The 16S rDNA primer set also amplified sequences that were not related to the known Nitrosospira-Nitrosomonas group, although they showed a close relationship with other groups of β-proteobacteria. The results suggest that Nitrosospira-like populations are dominant AOB populations in the submerged paddy soil, and that the oxic layer of submerged paddy soil harbours the specific AOB. 相似文献
79.
(日池田知久 《湖南农业大学学报(自然科学版)》2013,(6):5-17
我曾经就睡虎地秦简《语书》中有关“乡俗”的思想做过探讨,明确了《语书》中的“乡俗”思想是受战国末期秦国的墨家“尚同论”的影响而写成的一种强化中央集权的政治思想。本文就有关中国古代“风俗”所持的诸思想中的Ａ与Ｂ的对立展开讨论。即：Ａ主张严格管制地方独自“风俗”的 《语书》、墨家、法家的中央集权思想以及儒家的“移风易俗”思想。Ｂ倡导天下、国家承认地方的各种“风俗”,并且应该依照其风俗施行政治的《淮南子·齐俗》篇等地方分权思想。 相似文献
80.
Nonawin Lucob-Agustin Tsubasa Kawai Mana Kano-Nakata Roel R. Suralta Jonathan M. Niones Tomomi Hasegawa Mayuko Inari-Ikeda Akira Yamauchi Yoshiaki Inukai 《Breeding Science》2021,71(1):20
Different types of water stress severely affect crop production, and the plant root system plays a critical role in stress avoidance. In the case of rice, a cereal crop cultivated under the widest range of soil hydrologic conditions, from irrigated anaerobic conditions to rainfed conditions, phenotypic root plasticity is of particular relevance. Recently, important plastic root traits under different water stress conditions, and their physiological and molecular mechanisms have been gradually understood. In this review, we summarize these plastic root traits and their contributions to dry matter production through enhancement of water uptake under different water stress conditions. We also discuss the physiological and molecular mechanisms regulating the phenotypic plasticity of root systems. 相似文献