The Effectiveness of Immunotherapy in Treating Exercise-Induced Pulmonary Hemorrhage     

Tammi S. Epp  Paul McDonough  Don E. Myers  Danielle J. Carlin  Brad J. Behnke  Casey A. Kindig  David C. Poole  Howard H. Erickson 《Journal of Equine Veterinary Science》2009

Inflammatory airway disease has been linked to exercise-induced pulmonary hemorrhage (EIPH), and consequently, we hypothesized that immunomodulation via concentrated equine serum (CES) treatment would reduce EIPH as evidenced by red blood cell (RBC) concentrations in bronchoalveolar lavage fluid (BALF). Separate trials were conducted on Thoroughbred horses treated with either CES (n = 6) or placebo (0.9% saline; n = 4). All horses completed pre-treatment and post-treatment (2 and 4 weeks after initiating treatment) maximal exercise tests on a 10% inclined treadmill (1 m/s/min increments to fatigue) over a 10-week period (2−3 weeks between tests), with bronchoalveolar lavage (BAL) performed 30 minutes after exercise. Treatment ensued 10 days after the pre-treatment exercise test, with horses receiving a series of five CES or placebo injections 24 hours apart (20 mL intratracheal and 10 mL intravenously), with subsequent weekly injections for 5 weeks thereafter. After CES treatment, both EIPH (RBC in BALF) and inflammation (white blood cell concentration [WBC] in BALF) were significantly diminished by the 4-week posttreatment run, demonstrating 46 ± 12% and 24 ± 11% decreases, respectively (P < 0.05). In contrast, EIPH was elevated significantly at the 4-week time point, and inflammation remained constant in the placebo trial. In conclusion, these preliminary data suggest that therapeutic intervention involving immunomodulation may represent a viable approach to reducing the severity of EIPH.  相似文献   

Gastrin-secreting neoplasia in a cat     

Diroff JS  Sanders NA  McDonough SP  Holt DE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(5):1245-1247

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Effect of Cryopreservation on Bovine Sperm Viability as Determined by Dual DNA Staining     

DL Garner  CA Thomas  CH Allen  PL Senger  RG Sasser 《Reproduction in domestic animals》1997,32(6):279-283

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Evaluation of protein digestibility-corrected amino acid score method for assessing protein quality of foods   总被引：5，自引：0，他引：5  

G Sarwar  F E McDonough 《Journal of the Association of Official Analytical Chemists》1990,73(3):347-356

The current concepts of protein quality evaluation were reviewed. A detailed examination of existing animal assays and more promising amino acid scoring methods has been carried out by an Ad Hoc Working Group on Protein Quality Measurement for the Codex Committee on Vegetable Proteins during the last 5 years. Several factors such as inadequacies of protein efficiency ratio (PER, the poorest test) and other animal assays, advancements made in standardizing methods for amino acid analysis and protein digestibility, availability of data on digestibility of protein and individual amino acids in a variety of foods, and reliability of human amino acid requirements and scoring patterns were evaluated. On the basis of this evaluation, amino acid score, corrected for true digestibility of protein, was recommended to be the most suitable routine method for predicting protein quality of foods for humans. Amino acid scores corrected for true digestibility of protein (as determined by rat balance method) were termed "protein digestibility-corrected amino acid scores." A detailed method for the determination of the protein digestibility-corrected amino acid score was proposed, and information about the range of scores to be expected in foods or food products was provided in the present investigation. The protein digestibility-corrected amino acid score method is a simple and scientifically sound approach for routine evaluation of protein quality of foods. Accuracy of the method would, however, be confirmed after validation with growth or metabolic balance studies in humans.  相似文献   

Evaluation of new leptospiral antigens for the diagnosis of equine leptospirosis: An approach using pan-genomic analysis,reverse vaccinology and antigenic selection     

Tiago J. Zilch  Jen-Jie Lee  Gustavo C. Bressan  Sean P. McDonough  Hussni O. Mohammed  Thomas J. Divers  Yung-Fu Chang 《Equine veterinary journal》2021,53(5):1025-1035

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Spinal cord nephroblastoma in dogs: 11 cases (1985-2007)     

Brewer DM  Cerda-Gonzalez S  Dewey CW  Diep AN  Van Horne K  McDonough SP 《Journal of the American Veterinary Medical Association》2011,238(5):618-624

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Geochemistry. Meteoritic clues point chromium toward Earth's core     

McDonough WF 《Science (New York, N.Y.)》2011,331(6023):1397-1398

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The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase     

Gerken T  Girard CA  Tung YC  Webby CJ  Saudek V  Hewitson KS  Yeo GS  McDonough MA  Cunliffe S  McNeill LA  Galvanovskis J  Rorsman P  Robins P  Prieur X  Coll AP  Ma M  Jovanovic Z  Farooqi IS  Sedgwick B  Barroso I  Lindahl T  Ponting CP  Ashcroft FM  O'Rahilly S  Schofield CJ 《Science (New York, N.Y.)》2007,318(5855):1469-1472

Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans. Here, we show by bioinformatics analysis that FTO shares sequence motifs with Fe(II)- and 2-oxoglutarate-dependent oxygenases. We find that recombinant murine Fto catalyzes the Fe(II)- and 2OG-dependent demethylation of 3-methylthymine in single-stranded DNA, with concomitant production of succinate, formaldehyde, and carbon dioxide. Consistent with a potential role in nucleic acid demethylation, Fto localizes to the nucleus in transfected cells. Studies of wild-type mice indicate that Fto messenger RNA (mRNA) is most abundant in the brain, particularly in hypothalamic nuclei governing energy balance, and that Fto mRNA levels in the arcuate nucleus are regulated by feeding and fasting. Studies can now be directed toward determining the physiologically relevant FTO substrate and how nucleic acid methylation status is linked to increased fat mass.  相似文献   

Geophysics. Mapping the Earth's engine     

McDonough WF 《Science (New York, N.Y.)》2007,317(5842):1177-1178

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Continuous infusion of propofol in dogs premedicated with methotrimeprazine   总被引：1，自引：0，他引：1  

Antonio JA Aguiar p  hd Stelio PL Luna p  hd Valéria NLS Oliva p  hd  Flávia R Eugênio MSc  Gladys B Castro p  hd 《Veterinary anaesthesia and analgesia》2001,28(4):220-224

Objective To evaluate the cardiopulmonary and clinical effects of three different infusion rates of propofol in dogs premedicated with methotrimeprazine. Study design Randomized experimental trial. Animals Ten healthy adult mixed‐breed male and female dogs, weighing from 14 to 20 kg. Methods Dogs were premedicated with methotrimeprazine [1 mg kg^?1 intravenously (IV)] followed by induction of anesthesia with 4.5 mg kg^?1 of propofol IV and maintenance with propofol for 60 minutes as follows: T1, 0.2 mg kg^?1 minute^?1; T2, 0.3 mg kg^?1minute^?1; and T3, 0.4 mg kg^?1minute^?1. Heart rate (HR), respiratory rate (RR), mean arterial pressure (MAP), end‐tidal CO₂ (P_ETCO₂), arterial hemoglobin O₂ saturation, arterial blood gases, and pedal and cutaneous reflexes were measured before and 5, 10, 20, 30, 45 and 60 minutes after the beginning of the propofol infusion. Statistical analysis was performed using an anova . Results Heart rate increased during anesthesia in all cases and arterial blood pressure decreased only in dogs in the T3 category. Respiratory depression was proportional to the infusion rate of propofol. Muscle relaxation was satisfactory, but analgesia was inadequate in the three treatments. Conclusions The infusion of 0.2–0.4 mg kg^?1 minute^?1 of propofol produced a dose‐dependent respiratory depression. The presence of a pedal withdrawal reflex and marked cardiovascular responses to this noxious stimulus suggests that anesthesia may not be of sufficient depth for surgery to be carried out. Clinical relevance Although several studies have been performed using propofol in animals, few studies have investigated the cardiopulmonary and analgesic effects with different doses. The determination of an adequate propofol infusion rate is necessary for the routine use of this intravenous anesthetic for the maintenance of anesthesia during major surgical procedures in dogs.  相似文献