Davies Keir Johnston     

RG Pedrana 《Australian veterinary journal》2001,79(9):633-633

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Effect of Cryopreservation on Bovine Sperm Viability as Determined by Dual DNA Staining     

DL Garner  CA Thomas  CH Allen  PL Senger  RG Sasser 《Reproduction in domestic animals》1997,32(6):279-283

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Description of the Canadian regional climate model     

D. Caya  R. Laprise  M. Giguère  G. Bergeron  J. P. Blanchet  B. J. Stocks  G. J. Boer  N. A. McFarlane 《Water, air, and soil pollution》1995,82(1-2):477-482

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DNA hybridization procedure to detect pseudorabies virus DNA in swine tissue   总被引：1，自引：0，他引：1  

R G McFarlane  D G Thawley 《American journal of veterinary research》1985,46(5):1133-1136

A DNA hybridization technique was developed to detect the presence of pseudorabies virus (PRV) DNA. P Nick translated probes of high specific activity were prepared from transformed Escherichia coli plasmids into which Bacillus amyloliquefaciens H (Bam H1) restriction fragments of PRV DNA had been inserted. Swine cellular DNA and tissue culture PRV DNA were digested with Bam H1, separated by agarosegel electrophoresis, transferred onto nitrocellulose paper, hybridized to the radioactive probes, and washed under high stringency conditions; autoradiographs were then prepared. Under the optimal hybridization conditions described, the detection limit of these probes was 10(-11)g of PRV DNA. In reconstruction experiments, 3 of the selected probes cross hybridized with digested swine cellular DNA, and 4 probes did not. The addition of polyuridylic acid and polyguanylic acid to the hybridization reactions did not alter the amount of hybridization. The results indicated that this procedure may be useful for studying the latency of pseudorabies viral infection.  相似文献   

Periodontal disease in free-ranging koalas (Phascolarctos cinereus) from the Mount Lofty Ranges,South Australia,and its association with koala retrovirus infection     

RG Butcher  LM Pettett  J Fabijan  E Ebrahimie  M Mohammadi-Dehcheshmeh  KN Speight  WSJ Boardman  PS Bird  DJ Trott 《Australian veterinary journal》2020,98(5):200-206

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Phylogeography,genetic stocks,and conservation implications for an Australian endemic marine turtle     

Nancy N. FitzSimmons  Stewart D. Pittard  Nathan McIntyre  Michael P. Jensen  Mick Guinea  Mark Hamann  Rod Kennett  Brett Leis  Colin J. Limpus  Duncan J. Limpus  Megan J. McCann  Anna J. MacDonald  Glenn McFarlane  C. John Parmenter  Kellie Pendoley  Robert T. Prince  Leigh Scheltinga  Kathrin Theissinger  Anton D. Tucker  David Waayers  Andrea Whiting  Scott Whiting 《水产资源保护:海洋与淡水生态系统》2020,30(3):440-460

1. Identification of the geographic extent of population boundaries, the distribution of genetic lineages, and the amount of genetic exchange among breeding groups is needed for effective conservation of vulnerable marine migratory species. This is particularly true of the flatback turtle (Natator depressus), which only breeds in Australia but has extensive migrations that can include international waters.
2. This study investigated the phylogeography and genetic structure among 17 flatback turtle rookeries across their range by sequencing an 810 bp portion of the mitochondrial DNA in 889 samples and genotyping 10 microsatellite loci in 598 samples.
3. There was low phylogenetic divergence among haplotypes and evidence of recent population expansion, likely in the late Pleistocene. A predominant haplotype was found across all rookeries, but other haplotype groups were regionally specific.
4. In general, there was agreement in patterns of genetic differentiation in the mitochondrial DNA and microsatellite data, and in some pairwise comparisons a higher mutation rate of microsatellites provided stronger evidence of differentiation.
5. These results suggest natal philopatry operates in the choice of breeding locations for males as well as females.
6. Evidence of genetic connectivity among neighbouring rookeries led to the identification of seven genetic stocks. Geographic boundaries of rookeries used by genetic stocks varied widely (160–1,300 km), highlighting a need for field studies to better understand movement patterns.
7. Hierarchical analysis of molecular variance identified significant genetic differentiation based upon genetic stock, nesting phenology (summer vs. winter nesters), and a west–east discontinuity across Torres Strait. A pattern of isolation by distance was identified, which was most strongly observed in the microsatellite data.
8. In combination with tagging and telemetry studies, these results will allow better quantification of stock‐specific threats along migratory routes and in foraging habitats. Implications of climate change will be stock specific and may depend upon the extent of genetic connectivity between neighbouring stocks.

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Suspected fatal venous air embolism during anaesthesia in a Pomeranian dog with pulmonary calcification     

VP Walsh  RG Machon  JS Munday  CJ Broome 《New Zealand veterinary journal》2013,61(5):359-362

CASE HISTORY: Death occurred in a 1.25 kg, 9-month-old female Pomeranian dog undergoing anaesthesia for surgical repair of partially healed fractures of the radius and ulna.

CLINICAL FINDINGS: Following sedation, anaesthesia was induced using thiopentone and maintained with halothane in oxygen. An acute decrease in the dog's end-tidal carbon dioxide (EtCO₂) measurements was noted approximately 50 min after induction, immediately following delivery of a 5-ml bolus of lactated Ringer's solution (LRS) administered to flush a small (0.06 ml) volume of fentanyl via a pre-placed intravenous (I/V) catheter. Venous air embolism (VAE) was suspected and the dog died despite interventive therapy. On post-mortem examination, several air bubbles were noted when the right ventricle was opened under water. Histologically, the kidneys appeared abnormal with immature glomeruli, and the lungs appeared diffusely mineralised. The origin of the air was probably small bubbles and microbubbles that may have been present in the extension set and 20 ml syringe used for the administration of fentanyl and I/V fluids to the dog.

DIAGNOSIS: Renal dysplasia and diffuse pulmonary calcification, with VAE as the probable cause of death.

CLINICAL RELEVANCE: In this case of VAE-associated anaesthetic death, it is further speculated that underlying pulmonary disease, in the form of pulmonary calcification, may have contributed to an increased sensitivity to the adverse effects of VAE.  相似文献   

Emerging technologies for identifying superior dairy cows in New Zealand     

DJ Garrick  RG Snell 《New Zealand veterinary journal》2013,61(6):390-399

The performance of animals is determined by the interaction of their genes with environmental circumstances. Accordingly, animals exhibiting superior performance are not necessarily the animals with the best genes nor are they the best choice of parents. Statistical analyses of production records for repeated traits, e.g. lactation yields and reproductive performance, show that part of the variation in performance among animals in the same herd and year is due to genetic differences, and the remainder is due to so-called residual or environmental factors that are not passed on to offspring. These within-herd environmental factors can be partitioned into a component that affects performance throughout an animal's lifetime, and a part that is unique to each observation. The process of animal evaluation from pedigree and performance records partitions the superiority of each cow into these three components. Reliable assessment of the genetic merit of bulls has required progeny testing, and for cows has required observation of their own individual performance. Selection on the genetic or breeding value component has systematically improved animal performance over recent decades, but has been limited by the age at which assessments of genetic merit are available.

Emerging molecular technologies can read DNA sequences or measure RNA expression and have allowed the identification of a number of chromosome regions, and a few specific genes in those regions, that influence economic performance. This information allows better characterisation of the relationships between animals and more accurate predictions of genetic merit in bulls without progeny information and in cows that have yet to produce their own performance record. At some stage, enough genes responsible for variation in performance will be identified to allow faster genetic progress through selection of animals at young ages and therefore more rapid turnover of the generations. Mechanisms that modify gene expression have been identified and these may ultimately allow animals to be selected at an early age for lifetime productivity, accounting for processes that modify gene expression and lead to differences in performance that are not reflected by DNA sequence information. This review describes the status of these emerging technologies and their likely role in the improvement of dairy cattle.  相似文献   

Abortions in sheep caused by Salmonella Brandenburg: Pathological findings     

RG Clark  JM Gill  RA Fairley  JA Smart 《New Zealand veterinary journal》2013,61(6):356-357

CASE HISTORY: A 7-year-old cat developed sporadic vomiting, reduced appetite, and weight loss over the previous 3 months.

CLINICAL FINDINGS: Palpation revealed a large mid-abdominal mass and the cat had marked eosinophilia. The cat progressively lost weight over the next 7 weeks when euthanasia was performed.

PATHOLOGICAL FINDINGS: Necropsy revealed a 3?cm diameter firm white intramural mass in the colon and another in the pylorus. Mesenteric and cranial mediastinal lymph nodes were firm, pale, and enlarged. Histopathological examination revealed foci of necrosis surrounded by thick dense collagen trabeculae and predominantly eosinophilic inflammation within the intestine and lymph nodes. Marked eosinophilic infiltration of the liver was also present.

DIAGNOSIS: The lesions were consistent with gastrointestinal eosinophilic sclerosing fibroplasia (FGESF).

CLINICAL RELEVANCE: This is the first report of FGESF in a New Zealand cat and the first time lesions of FGESF have been observed in extra-abdominal tissues. Intestinal neoplasia can be clinically identical to FGESF and histopathology is required for differentiation. Evidence suggests that FGESF has a more favourable prognosis than intestinal neoplasia.  相似文献   

The artificial infection of sheep with a brucella-like organism     

W.J. Hartley M.R.C.V.S.  J.L. Jebson B.V.Sc.  D. McFarlane B.Sc. M.R.C.V.S. 《New Zealand veterinary journal》2013,61(3):80-89

Extract

During the course of an investigation into perinatal calf deaths in the Gisborne area during the spring of 1958, seven cases of micrencephaly were recorded.  相似文献