The pathogenicity of canine parvovirus type-2b, FP84 strain isolated from a domestic cat, in domestic cats     

Gamoh K  Shimazaki Y  Makie H  Senda M  Itoh O  Inoue Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):1027-1029

Canine parvovirus type-2a (CPV-2a) and type-2b (CPV-2b) have recently been isolated from domestic cats. The pathogenicity of CPV-2b in domestic cats is still unclear. In this study, we performed infection tests to examine the pathogenicity of CPV-2b, FP84 strain, isolated from a domestic cat. The results demonstrated that the CPV strain FP84 is able to infect and replicate well in domestic cats. Two of the 3 cats used in the test died. They showed loss of appetite, diarrhea, leukopenia and dehydration. Since FP84 was found to be virulent to domestic cats, it is necessary to examine the efficacy of inactivated feline panleukopenia virus vaccines against CPV infection in domestic cats.  相似文献   

Somaclonal variation in Tricyrtis hirta plants regenerated from 1-year-old embryogenic callus cultures     

Masaru Nakano  Toshikazu Nomizu  Keiko Mizunashi  Mami Suzuki  Shiro Mori  Sachiko Kuwayama  Megumi Hayashi  Hiroto Umehara  Eriko Oka  Hiroyuki Kobayashi  Megumi Asano  Shintaro Sugawara  Hiroki Takagi  Hiroyuki Saito  Masashi Nakata  Toshinari Godo  Yoshihiro Hara  Junji Amano 《Scientia Horticulturae》2006,110(4):366-371

The Liliaceous perennial Tricyrtis hirta, sometimes called ‘Japanese toad lily’, has recently become popular as an ornamental for pot and garden uses. Highly embryogenic callus cultures of this plant predominately consisted of diploid cells but also contained tetraploid cells after 1 year of establishment. In the present study, plans regenerated from the 1-year-old embryogenic callus cultures were subjected to ploidy level analysis and morphological characterization following 3 years of cultivation. Among 37 plants examined, 28 kept the diploid level (2n = 2x = 26) but nine were tetraploid (2n = 4x = 52) as indicated by FCM analysis and chromosome observation. Although no morphological alterations were detected in 26 out of 28 diploid regenerants, the remaining two showed noticeable variations: both were severely dwarf and had crimped leaves and many malformed flowers. The tetraploid regenerants had several horticulturally attractive characteristics compared with the diploid controls, such as longer shoots, thicker stems, and larger flowers. Thus regeneration of tetraploid plants from 1-year-old embryogenic callus cultures offers a possibility to improve the horticultural value of T. hirta, although regeneration of trueness-to-type plants is essential for utilizing the cultures for micropropagation and genetic transformation.  相似文献   

Induction of defense responses in pea tissues by inorganic phosphate     

Tomoharu Kawahara  Hiroko Namba  Kazuhiro Toyoda  Tomonari Kasai  Megumi Sugimoto  Yoshishige Inagaki  Yuki Ichinose  Tomonori Shiraishi 《Journal of General Plant Pathology》2006,72(3):129-136

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Antibacterial activity of bovine lactoferrin hydrolysate against mastitis pathogens and its effect on superoxide production of bovine neutrophils     

Kawai K  Shimazaki K  Higuchi H  Nagahata H 《Zoonoses and public health》2007,54(3-4):160-164

Antibacterial activity of bovine lactoferrin hydrolysates (LFH) on microorganisms isolated from bovine mastitis, and superoxide (O(2)(-)) production of bovine neutrophils were evaluated. Antibacterial effects of LFH were measured in vitro against Staphylococcus aureus, coagulase-negative staphylococci, Streptococci, Enterococci, Escherichia coli, Klebsiella pneumoniae, yeast-like fungi and Prototheca zopfii isolated from clinical cases of bovine mastitis. To compare susceptibilities against LFH, minimal inhibitory concentration (MIC) values were determined by a micro-plate assay method. Most organisms were sensitive to LFH. Prototheca zopfii was highly sensitive to LFH; the growth of the microorganism was inhibited completely even at 1 mug/ml. Staphylococcus aureus and Escherichia coli were resistant to LFH. The production of O(2)(-) by bovine neutrophils was used to evaluate the effect of LFH administration on functional activity. Increase in O(2)(-) production by bovine neutrophils occurred upon addition of LFH to neutrophils. These results demonstrate that LFH possesses antibacterial activity against pathogens that cause mastitis and activates neutrophil superoxide production.  相似文献   

Radial variations of wood properties in <Emphasis Type="Italic">Casuarina equisetifolia</Emphasis> growing in Bangladesh     

Md Qumruzzaman Chowdhury  Futoshi Ishiguri  Kazuya Iizuka  Yuuya Takashima  Kahoru Matsumoto  Tokiko Hiraiwa  Megumi Ishido  Hiroyuki Sanpe  Shinso Yokota  Nobuo Yoshizawa 《Journal of Wood Science》2009,55(2):139-143

Radial variations of wood properties (basic density, fiber length, vessel element length, and compression strength) in plantation-grown Casuarina equisetifolia in Bangladesh were investigated for effective utilization of the wood. Samples disks at breast height were randomly collected from trees in a 10-year-old plantation in Cox’s Bazar Forest Division, Bangladesh. The basic density showed a near-constant value up to 30 mm from the pith and then rapidly increased up to 60 mm from the pith. The fiber length and vessel element length gradually increased from the pith to bark. When radial variation of wood properties was determined according to relative distance from the pith, similar radial patterns were observed among the sample trees, indicating that the wood properties in C. equisetifolia may be related to the growth rate. The compression strength parallel to the grain (CS) increased from the pith to bark. A significant positive correlation was found between the air-dried density and the CS. The results obtained indicated that wood around the pith has a relatively low density, and wood outside the pith area has a relatively high density, suggesting that it could be used as structural lumber. Part of this report was presented at the 58th Annual Meeting of Japan Wood Research Society, Tsukuba, March 2008  相似文献   

Properties of extracts from defatted rice bran by its subcritical water treatment   总被引：2，自引：0，他引：2  

Wiboonsirikul J  Kimura Y  Kadota M  Morita H  Tsuno T  Adachi S 《Journal of agricultural and food chemistry》2007,55(21):8759-8765

Defatted rice bran was extracted with water and subcritical water at 50-250 degrees C for 5 min. The highest extract yield was achieved at 200 degrees C, at which the maximum amounts of protein and carbohydrate were also obtained. The total phenolic and furfural contents, radical scavenging activity, and antioxidative activity for the autoxidation of linoleic acid increased with increasing treatment temperature. The bran extracts exhibited emulsifying activity except for the extract prepared at 250 degrees C, which was concomitant with the disappearance of its high-molecular-mass substances. The extract prepared at 200 degrees C also had the highest emulsion-stabilizing activity.  相似文献   

Difructose anhydride III promotes absorption of the soluble flavonoid alphaG-rutin in rats     

Matsumoto M  Matsukawa N  Chiji H  Hara H 《Journal of agricultural and food chemistry》2007,55(10):4202-4208

A highly soluble quercetin glycoside, alphaG-rutin, is a glucose adduct of insoluble rutin. We examined the effects of difructose anhydride III (DFAIII; di-D-fructofuranosyl 1,2':2,3'-dianhydride) on intestinal absorption of alphaG-rutin by rat experiments. alphaG-rutin, rutin, quercetin, and the quercetin conjugate appeared in the portal blood after an intubation of alphaG-rutin (100 micromol), DFAIII effected higher portal concentrations of alphaG-rutin in portal cannulated rats. In ligated jejunal and ileal loops of rats, alphaG-rutin, rutin, quercetin, and the quercetin conjugate appeared in the intestinal mesenteric blood at both 30 and 60 min in both loops; the concentration of alphaG-rutin was 1.5-3 times higher when absorbed in the presence DFAIII. In the isolated mucosae of the jejunum and ileum, mucosal-to-serosal passage of alphaG-rutin increased with the addition of 100 micromol of DFAIII. These results indicate that alphaG-rutin is absorbed as the intact form and that DFAIII stimulates its absorption in the small intestine.  相似文献   

Spinal cord trauma in a cat caused by ingestion of a splinter     

Tanaka T  Akiyoshi H  Shimazaki H  Sato A  Inada M  Kiyomiya K  Shimizu J  Cao Z  Li Y  Hayashi A  Sone K  Kitai T  Ohashi F 《Journal of Feline Medicine and Surgery》2012,14(2):155-157

A 5-month-old male Abyssinian presented with right hemiplegia. Cervical radiographs, haematology and serum biochemistry were normal. A foreign body was identified on computed tomography (CT) and magnetic resonance imaging (MRI). Removal of the foreign body was carried out via a dorsal approach between the occipital bone and the C1 vertebral body using intra-operative CT. No complications were encountered. Thirty days after surgery, the cat was ambulatory.  相似文献   

Extracellular cyclophilin A possesses chemotaxic activity in cattle     

Satoru Takanashi  Tomonori Nochi  Miku Abe  Nanami Itaya  Megumi Urakawa  Katsuyoshi Sato  Tao Zhuang  Saori Umemura  Tomohito Hayashi  Yoshio Kiku  Haruki Kitazawa  Michael T. Rose  Kouichi Watanabe  Hisashi Aso 《Veterinary research》2015,46(1)

Cyclophilin A (CyPA) was originally discovered in bovine thymocytes as a cytosolic binding protein of the immunosuppressive drug cyclosporine A. Recent studies have revealed that in mice and humans, CyPA is secreted from cells in injured or infected tissues and plays a role in recruiting inflammatory cells in those tissues. Here we found that in cattle abundant level of extracellular CyPA was observed in tissues with inflammation. To aid in investigating the role of extracellular CyPA in cattle, we generated recombinant bovine CyPA (rbCyPA) and tested its biological activity as an inflammatory mediator. When bovine peripheral blood cells were treated with rbCyPA in vitro, we observed that rbCyPA reacts with the membranous surface of granulocytes, monocytes and lymphocytes. Chemotaxis analysis showed that the granulocytes migrate toward rbCyPA and the migration is inhibited by pre-treatment with an anti-bovine CyPA antibody. These results indicate that, as for mice and humans, extracellular CyPA possesses chemotactic activity to recruit inflammatory cells (e.g., granulocytes) in cattle, and could thus be a potential therapeutic target for the treatment of inflammation.  相似文献   

Visfatin: a protein secreted by visceral fat that mimics the effects of insulin     

Fukuhara A  Matsuda M  Nishizawa M  Segawa K  Tanaka M  Kishimoto K  Matsuki Y  Murakami M  Ichisaka T  Murakami H  Watanabe E  Takagi T  Akiyoshi M  Ohtsubo T  Kihara S  Yamashita S  Makishima M  Funahashi T  Yamanaka S  Hiramatsu R  Matsuzawa Y  Shimomura I 《Science (New York, N.Y.)》2005,307(5708):426-430

Fat tissue produces a variety of secreted proteins (adipocytokines) with important roles in metabolism. We isolated a newly identified adipocytokine, visfatin, that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin corresponds to a protein identified previously as pre-B cell colony-enhancing factor (PBEF), a 52-kilodalton cytokine expressed in lymphocytes. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. Mice heterozygous for a targeted mutation in the visfatin gene had modestly higher levels of plasma glucose relative to wild-type littermates. Surprisingly, visfatin binds to and activates the insulin receptor. Further study of visfatin's physiological role may lead to new insights into glucose homeostasis and/or new therapies for metabolic disorders such as diabetes.  相似文献