Relationship between change of hypoxic tissue and vascular proliferation after cerebral infarction in rats     

PAN Jing-rui  WANG Yi-dong  LI Mei  L Rui-yan  QIU Yu  PENG Ying 《园艺学报》2009,25(10):1926-1930

AIM: To clarify the relationship between the change of hypoxic tissue and vascular proliferation after cerebral infarction. METHODS: Rats with middle cerebral artery occlusion were randomly divided into 1.5 h ischemia-reperfusion (1.5 h IR) group and permanent ischemia (PI) group. Double staining by immunofluorescence was used to identify the hypoxic tissue and microvessels by EF5 and vWF labelings, respectively. On 3 d, 7 d and 14 d post-operation, microvessel density (MVD) and hypoxic tissue in ischemic cortex were observed. RESULTS: Hypoxic tissues existed from 3 d to 14 d post-operation in 1.5 h IR group and disappeared after 3 d in PI group. The MVD within hypoxic tissue was smaller than that around it at all observed time points (all P<0.01). With the lapse of time, MVD in ischemic cortex gradually increased both in 1.5 IR and PI groups (all P<0.01) and was bigger in 1.5 h IR group than that in PI group on 7 d and 14 d (both P<0.01). CONCLUSION: Hypoxic tissues mainly locate in areas of sparse microvessel after cerebral infarction and their duration is partially dependent on the grade of microvessel proliferation in ischemic cortex.  相似文献   

Prokaryotic expression and identification of a small peptide of osteopontin     

LIU Ying-hui  TIAN Wen-jia  ZHENG Bin  HAN Mei  WEN Jin-kun 《园艺学报》2009,25(5):848-852

AIM: To express osteopontin 13 peptide (OPN 13) in E.coli, and to test the biological activity of the purified products. METHODS: cDNA fragments containing RGD sequences were cloned into prokaryotic expression vector pET-32c(+) including His coding sequence to construct pET-32c-OPN 13 plasmid. E.coli DH5α transformed by pET-32c-OPN 13 plasmid was induced by IPTG at different concentrations for different times to identify the optimal induction condition. Expressed His-OPN 13 fusion protein was purified via Ni-NTA His Bind Resin metal chelation chromatography, and detected by VSMCs adhesion and migration analysis. RESULTS: His-OPN 13 fusion protein was expressed in soluble manner. The fusion proteins were purified via Ni-NTA His Bind Resin affinity chromatography. His-OPN 13 fusion protein specifically inhibited adhesion and migration of VSMCs stimulated by osteopontin in dose-dependent manner. CONCLUSION: The OPN 13 peptide is successfully expressed in E.coli DH5α. The purified His-OPN 13 fusion protein could inhibit the adhesion and migration of VSMCs stimulated by osteopontin.  相似文献   

PEG对10℃低温吸胀下实葶葱种子萌发的影响     

史梅  谭敦炎  林辰壹  尹旭 《北方园艺》2009,(10)

基于种子萌发试验,测定了10℃低温对实葶葱种子发芽的影响及浓度为0%、10%、15%、20%、25%、30%的PEG处理对实葶葱种子低温吸胀1、2、3、5、7 d的缓解作用.结果表明:10℃低温吸胀持续时间超过临界时间(1 d)时就会抑制实葶葱种子的萌发.10℃低温吸胀通过PEG的渗透调节,可显著改善实葶葱种子的萌发,其中以处理浓度15%时效果最好,并随着低温吸胀时间的延长PEG的作用越明显.各浓度PEG处理10℃低温吸胀的实葶葱种子1d时种子萌发效果最佳.  相似文献   

沂州木瓜外植体灭菌技术研究     

王梅  姜峰  陈火星 《长江蔬菜》2009,(24)

利用沂州木瓜新长出10 d左右的嫩茎,采用不同消毒剂、灭菌时间对沂州木瓜进行初代培养试验,结果表明,沂州木瓜外植体的消毒在75%酒精10 s和0.1%升汞1.5 min的条件下效果最佳.  相似文献   

草菇菌丝优化培养条件研究   总被引：2，自引：1，他引：1  

伍国明  李梅 《中国食用菌》2009,28(3):28-31

试验研究不同营养因子与培养条件(pH值、温度)对草菇4个菌株菌丝生长的影响.结果表明,菌丝生长最佳碳源为蔗糖,最佳氮源为蛋白胨,无机盐为硫酸镁,植物生长素为萘乙酸,最适培养温度为30℃～35℃,最适pH值范围为7.0～11.0.正交试验确定最佳培养基组分为蔗糖20.0 g·L-1、蛋白胨5.0 g·L-1、硫酸镁1.5 g·L-1、萘乙酸5.0 mg·L-1、马铃薯200 g·L-1.  相似文献   

渝东南岩溶区地下河水质情况调查   总被引：1，自引：0，他引：1  

杨梅  扈志勇  蒲俊兵 《中国农村水利水电》2009,(2):12-14

中国南方地下河分布广泛,储量极为丰富,仅在广西、贵州、云南、四川和湖南五省区,枯季流量大于50L/s的地下河或伏流就有2836条。岩溶地下河的水质是一个应当引起重视的问题。通过调查武隆、彭水和涪陵三个地区的主要地下河,并与我国地下水质量标准(GB/T14848-93)进行对照。结果发现大部分地下河均受到不同程度的污染,地下河的污染会影响到人们正常的饮水安全问题,因此,要引起我们的高度重视。  相似文献   

光唇鱼网箱养殖试验   总被引：2，自引：0，他引：2  

黄富友  苏小平  梅金满 《水产养殖》2008,29(1):8-10

网箱单养、混养光唇鱼的试验结果表明,在25m2网箱中,单养光唇鱼以200～220尾/m2的放养密度比较合适,单位面积产量可达12.7～12.9 kg/m2;瓯江彩鲤网箱中混养光唇鱼,在瓯江彩鲤保持180尾/m2的放养密度不变的情况下,混养20尾/m2的光唇鱼,其效果比单养瓯江彩鲤更好,单位面积产量可达103.2kg/m2.  相似文献   

早熟、高产转基因抗虫常规棉品种——湘K25   总被引：1，自引：1，他引：0  

贺云新  张志刚  梅正鼎  张兴平  吴佶膛  蒋杰 《中国棉花》2017,44(6):33-34

概述了湘K25的选育经过,介绍了湘K25的特征特性、产量、纤维品质和抗病、抗虫等特点,并总结了其关键栽培技术。  相似文献   

中美德系玉米品种的耐密性对深松耕作措施的响应分析   总被引：1，自引：0，他引：1  

裴宽  于晓芳  高聚林  王志刚  孙继颖  胡树平  李雅剑  刘梅  赵强 《玉米科学》2017,25(4):97-104

采取耕作方式×种植密度×品种三因素裂区试验,深松40 cm和旋耕15 cm耕作条件,在9.0万株/hm~2和10.5万株/hm~2种植密度下,对中系郑单958和登海618、美系先玉335和华美1号、德系KX3564和KWS2564不同血缘玉米品种的地上部形态、生理及产量构成指标进行对比分析。结果表明,深松可降低增密后玉米净光合速率、LAI、SPAD值和单株生产能力的下降幅度,缓解株型的变化,提高玉米的耐密性。我国玉米品种的耐密性对深松耕作响应与美系和德系玉米品种存在差异,深松耕作与旋耕相比,华美1号、KX3564、KWS2564、先玉335增加种植密度后,其穗位叶叶倾角、净光合速率、LAI、SPAD值下降缓慢,产量、耐密系数显著提高,其耐密适应性对深松耕作的响应度高于郑单958、登海618。  相似文献   

Conferring resistance to pre-harvest sprouting in durum wheat by a QTL identified in <Emphasis Type="Italic">Triticum spelta</Emphasis>     

Yujiao Liu  Yaxi Liu  Yong Zhou  Charlene Wight  Zhien Pu  Pengfei Qi  Qiantao Jiang  Mei Deng  Zaoxia Wang  Yuming Wei  Wenguang Cao  Dengcai Liu  Youliang Zheng  Chunji Liu  Judith Frégeau-Reid  Jirui Wang 《Euphytica》2017,213(1):19

Pre-harvest sprouting (PHS) causes significant yield loss and degrade the end-use quality of wheat, especially in regions with prolonged wet weather during the harvesting season. Unfortunately, the gene pool of Triticum durum (tetraploid durum wheat) has narrow genetic base for PHS resistance. Therefore, finding out new genetic resources from other wheat species to develop PHS resistance in durum wheat is of importance. A major PHS resistance QTL, Qphs.sicau-3B.1, was mapped on chromosome 3BL in a recombinant inbred line population derived from ‘CSCR6’ (Triticum spelta), a PHS resistant hexaploid wheat and ‘Lang’, a PHS susceptible Australian hexaploid wheat cultivar. This QTL, Qphs.sicau-3B.1, is positioned between DArT marker wPt-3107 and wPt-6785. Two SCAR markers (Ph3B.1 and Ph3B.2) were developed to track this major QTL and were used to assay a BC₂F₈ tetraploid population derived from a cross between the durum wheat ‘Bellaroi’ (PHS susceptible) and ‘CSCR6’ (PHS resistant). Phenotypic assay and marker-assisted selection revealed five stable tetraploid lines were highly PHS resistant. This study has successfully established that PHS-resistance QTL from hexaploid wheat could be efficiently introgressed into tetraploid durum wheat. This tetraploid wheat germplasm could be useful in developing PHS resistant durum cultivars with higher yield and good end-use quality.  相似文献