草菇低温诱导蛋白的初步研究   总被引：4，自引：2，他引：2  

张Meng  陈明杰 《食用菌学报》2000,7(1):56-58

低温诱导草菇（Ｖｏｌｖａｒｉｅｌｌａ ｖｏｌｖａｃｅａ）菌丝体一时间后,发现草菇在低温胁迫中产生了新的可溶性蛋白质。应用电泳分离制备技术,分离纯化了草菇菌丝体中的一个低温诱导蛋白。  相似文献   

Nutrition for Sick Horses     

Men Stratton-Phelps DVM 《Journal of Equine Veterinary Science》2003,23(8)

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Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice     

Hiroyuki Kaneko  Kazuhiro Kikuchi  Nguyen Thi Men  Junko Noguchi 《Animal Science Journal》2019,90(3):344-352

Ovarian xenografting makes it possible to obtain oocytes with fertilization ability from immature pigs of Western breeds. In this study, we applied these methods to the Meishan, an indigenous Chinese pig breed, and investigated the developmental competence of oocytes grown in their neonatal tissue after grafting into nude mice. First, mice harboring neonatal ovarian tissue were infused with follicle stimulating hormone (FSH) (62.5 U/ml) for 13 days starting at 10, 30, and 60 days after vaginal opening (D10‐, D30‐, and D60‐FSH groups, respectively). Development of antral follicles and their oocytes was most enhanced in the D60‐FSH group. For the next step, we examined the in vitro maturation ability of the oocytes recovered from host mice after infusion with FSH at a dose of 62.5 U/ml or 125 U/ml (FSH‐62.5 or ‐125 group) for 13 days starting at 60 days after vaginal opening. Many more oocytes with maturation ability were obtained from the FSH‐125 group. The FSH‐125 mature oocytes were fertilized in vitro, as shown by formation of male and female pronuclei, but did not reach the blastocyst stage. These results indicate that Meishan neonatal ovaries are able to produce oocytes with fertilization ability after being grafted into nude mice.  相似文献   

scRNA-seq of ovarian follicle granulosa cells from different fertility goats reveals distinct expression patterns     

Zengkuan Li  Junjie Wang  Yong Zhao  Dongxue Ma  Minghui Zhao  Na Li  Yuhao Men  Yuan Zhang  Huimin Chu  Chuzhao Lei  Wei Shen  Othman El-Mahdy Othman  Lingjiang Min 《Reproduction in domestic animals》2021,56(5):801-811

The new technology of high-throughput single-cell RNA sequencing (10 × scRNA-seq) was developed recently with many advantages. However, it was not commonly used in farm animal research. There are few reports for the gene expression of goat ovarian follicle granulosa cells (GCs) during different developmental stages. In the current investigation, the gene expression of follicle GCs at different stages from two populations of Ji'ning grey goats: high litter size (HL; ≥3/L; 2 L) and low litter size (LL; ≤2 /L; 2 L) were analysed by scRNA-seq. Many GC marker genes were identified, and the pseudo-time showed that GCs developed during the time course which reflected the follicular development and differentiation trajectory. Moreover, the gene expression difference between the two populations HL versus LL was very clear at different developmental stages. Many marker genes differentially expressed at different developmental stages. ASIP and ASPN were found to be highly expressed in the early stage of GCs, INHA, INHBA, MFGE8 and HSD17B1 were identified to be highly expressed in the growing stage of GCs, while IGFBP2, IGFBP5 and CYP11A1 were found to be highly expressed in late stage. These marker genes could be used as reference genes of goat follicle GC development. This investigation for the first time discovered the gene expression patterns in goat follicle GCs in high- or low-fertility populations (based on litter size) by scRNA-seq which may be useful for uncovering the oocyte development potential.  相似文献   

A novel ambipolar‐resin membrane for extracting soil nutrients     

Yu‐ying Men  Li‐yuan He  Ai‐qun Zhang  Benny K. G. Theng  Hua‐bo Liu  Zai‐hua Guo 《植物养料与土壤学杂志》2010,173(2):198-206

We have synthesized a novel ambipolar membrane for the simple, rapid, and simultaneous extraction of key nutrients from soil. The membrane was made by adding an anion‐ and a cation‐exchange resin to a polyvinyl alcohol hydrogel in the presence of glutaraldehyde as a cross‐linking agent. The synthetic membrane was efficient in adsorbing (extracting) NO , PO , K⁺, Ca²⁺, and Mg²⁺ ions from soil simultaneously. The ion‐adsorption capacity of the membrane was related to the soil nutrient status, duration of membrane–soil contact, and soil water content. The importance of these factors followed the order: soil nutrient status > contact time > soil water content. Adsorption by the membrane of NO and Mg²⁺ ions from soil leveled off after 48 h of membrane–soil contact but uptake of Ca²⁺, PO , and K⁺ ions required a longer contact time for equilibrium to be established. When the soil water content exceeds 55% w/w, this factor ceased to influence ion adsorption by the ambipolar‐resin membrane. The synthetic membrane is potentially useful for the in situ assessment of the nutrient requirement of certain crops at a given point in time.  相似文献   

植物器官体积变化连续测微法指导果树自动化灌溉合理指标的研究     

李绍华  余克顺  孟昭清  罗国光 《果树学报》1999,16(3)

对盆栽葡萄（VitisviniferaL．）、苹果（MaluspumilaMill．）、山楂（CrataeguspinnatifidsBunge）和枣（ZiziphusjnyubaMill．）4种果树在不同水分营养状况下茎于微变化过程中的日最大收缩量（MDS）、日净增长量（DG）、当日完全复原所需时间（RT）等3个重要指标进行研究，结果表明：3个指标都比叶片日出前水势对土壤水分营养状况的反应灵敏得多；随着土壤可利用水分的不断减少，山楂和枣的MDS急剧增加，而葡萄和苹果的MDS减小，不同类型果树之间的反应不一致；在土壤水分状况较好时，4种果树的DG的变化平缓，变化幅度较小；在良好的土壤水分条件下，4种果树的RT随着土壤相对持水量的下降而迅速延长，并在土壤水分含量降低到一临界值以下时维持在24h。因此，在应用植物器官体积变化连续测微法指导果树灌溉时，MDS和DG并不适于单独作为所有果树的自动化灌溉指标，而RT作为果树自动化灌溉指标较为理想。  相似文献   

果树新害虫叔白颜果蝇Drosophila triauraria在山东省的发生规律     

吕召云  高欢欢  翟一凡  刘莎  门兴元  于毅 《植物保护学报》2019,46(3):711-712

正果蝇属双翅目果蝇科果蝇属昆虫,多数种类仅以腐烂果实为食物,少数可为害健康果实。已报道的为害多种水果的果蝇有斑翅果蝇Drosophila suzukii、黑腹果蝇D. melanogaster等(任路明等,2014)。2017年用装有糖醋酒液的诱捕器在山东省烟台市、泰安市、济南市、日照市4个市的樱桃园和葡萄园收集果蝇,调查发现一种新的果蝇——叔白  相似文献   

原木边部锯材的精确尺寸和相应规格的确定     

门泽民 《西北林学院学报》1990,5(1):60-65

确定了原木边部锯材宽度和厚度的理论值0.424d×0.1d,较目前通用的0.43d×0.1d和0.421d×0.1d均更精确。根据确定式计算的边部锯材出材率,平均为17.66％;边部规格锯材出材率为17.49％;而边部Ⅰ等钝棱锯材的出材率则达23.48％。  相似文献   

原木周边部分的最大短锯材     

门泽民 《西北林学院学报》1992,7(2):31-33

对原木周边部分最大短锯材材积计算式和最佳锯剖位置探索的结果。最大短锯材材积计算式为: V=(43~(1/2)(R~2-a~2)~(3/2))/(R~2-r~2)(a-b)L最大短锯材外材面的最佳位置为: a=(3b±(9b~2+16R~2)~(1/2))/8  相似文献   

对应分析方法的注记   总被引：7，自引：0，他引：7  

孟德顺  郭满才 《西北林学院学报》1996,11(3):78-81

本文说明了对应分析中变量的协方差阵是一个退化矩阵。阐述了要进行对应分析时,原始数据应满足的条件。通过讨论得出进行对应分析若需要对原始数据作标准化处理时,模标准化、最大值标准化和总和标准化总是可行的,应用极差标准化是有条件的;而中心化、离差标准化和标准差标准化则不可使用  相似文献