全文获取类型
| 收费全文 | 121篇 |
| 免费 | 14篇 |
专业分类
| 林业 | 1篇 |
| 综合类 | 9篇 |
| 农作物 | 1篇 |
| 水产渔业 | 2篇 |
| 畜牧兽医 | 122篇 |
出版年
| 2023年 | 2篇 |
| 2020年 | 1篇 |
| 2019年 | 1篇 |
| 2018年 | 4篇 |
| 2017年 | 4篇 |
| 2016年 | 8篇 |
| 2015年 | 5篇 |
| 2014年 | 4篇 |
| 2013年 | 33篇 |
| 2012年 | 2篇 |
| 2011年 | 1篇 |
| 2010年 | 7篇 |
| 2009年 | 4篇 |
| 2008年 | 1篇 |
| 2007年 | 2篇 |
| 2006年 | 4篇 |
| 2005年 | 3篇 |
| 2004年 | 2篇 |
| 2003年 | 1篇 |
| 2001年 | 3篇 |
| 1999年 | 2篇 |
| 1998年 | 7篇 |
| 1997年 | 10篇 |
| 1996年 | 3篇 |
| 1995年 | 1篇 |
| 1994年 | 4篇 |
| 1993年 | 4篇 |
| 1992年 | 1篇 |
| 1991年 | 6篇 |
| 1990年 | 2篇 |
| 1989年 | 1篇 |
| 1988年 | 1篇 |
| 1986年 | 1篇 |
排序方式： 共有135条查询结果，搜索用时 265 毫秒
31.
Yano J Kern J Sauer K Latimer MJ Pushkar Y Biesiadka J Loll B Saenger W Messinger J Zouni A Yachandra VK 《Science (New York, N.Y.)》2006,314(5800):821-825
The oxidation of water to dioxygen is catalyzed within photosystem II (PSII) by a Mn(4)Ca cluster, the structure of which remains elusive. Polarized extended x-ray absorption fine structure (EXAFS) measurements on PSII single crystals constrain the Mn(4)Ca cluster geometry to a set of three similar high-resolution structures. Combining polarized EXAFS and x-ray diffraction data, the cluster was placed within PSII, taking into account the overall trend of the electron density of the metal site and the putative ligands. The structure of the cluster from the present study is unlike either the 3.0 or 3.5 angstrom-resolution x-ray structures or other previously proposed models. 相似文献
32.
S King C Favrot L Messinger T Nuttall J Steffan S Forster W Seewald 《Veterinary dermatology》2012,23(5):440-e84
Background – Hypersensitivity dermatitides (HD) are frequently suspected in cats, but there are few clinical studies on safe and effective treatments in the published literature. Objectives – To establish a safe and effective dose of ciclosporin in the treatment of feline HD. Animals – One hundred client‐owned cats with feline HD. Methods – Double‐blind study, with cats randomly assigned to receive ciclosporin at either 7.0 mg/kg once daily (n = 33) or 2.5 mg/kg once daily (n = 32) or a placebo (n = 35) for 6 weeks. Results – Mean Total Lesion Scores with 7.0 mg/kg ciclosporin were significantly lower than with 2.5 mg/kg ciclosporin (P = 0.0047) or placebo (P = 0.0003) at study end. Individual Total Lesion Scores improved by >50% in 70% of the 7.0 mg/kg group, compared with 47% in the 2.5 mg/kg group and 23% in the placebo group (P = 0.0006). The investigators’ Global Assessment of Improvement was ‘excellent’ or ‘good’ in 61% of cats treated with 7.0 mg/kg ciclosporin, compared with 47% of cats given 2.5 mg/kg and 23% given placebo. The improvement in Investigator Pruritus Scores was significantly greater in cats treated with 7.0 mg/kg ciclosporin (54%) compared with both 2.5 mg/kg ciclosporin (32%; P = 0.0232) and placebo (21%; P = 0.0063). Mild gastrointestinal disorders were the most common adverse events, but these did not require cessation of therapy. Conclusions and clinical importance – Results suggest that 7.0 mg/kg ciclosporin once daily in food or per os for 6 weeks is effective and well tolerated in feline HD. 相似文献
33.
MJ Lancaster JS Nimmo C Lenghaus IJ Gill RD Crawford RT Badman JL Samuel CJ Werner C Button N Kvalheim 《Australian veterinary journal》2009,87(12):476-479
Objective To document an ovine disease attributed to the consumption of Lythrum hyssopifolia (lesser loosestrife). Procedures Historical and histological review of field and experimental cases. Results 1–20% mortality occurred in sheep flocks grazing paddocks where L. hyssopifolia was the predominant green vegetation. Well‐documented disease outbreaks occurred in summer on nine farms across Victoria between 1974 and 2002. Liver damage occurred in all nine outbreaks, with kidney damage in at least eight. Hepatocyte necrosis was usually zonal to midzonal (zone 2) in the liver samples from four farms and periacinar (zone 3) in those from three farms, but some livers showed only single‐cell necrosis. Multinucleate hepatocytes near necrotic areas were a feature in six cases. Proximal tubular epithelium appeared to be the primary renal target and brown granules were often present in renal tubules. Biochemical and histological evidence of liver and kidney damage was obtained from two sheep experimentally pen‐fed harvested L. hyssopifolia. Conclusion Chemicals in L. hyssopifolia are toxic to ovine hepatocytes and renal tubular epithelial cells. 相似文献
34.
35.
JF Asturiano F Marco-Jiménez DS Peñaranda DL Garzón L Pérez JS Vicente M Jover 《Reproduction in domestic animals》2007,42(2):162-166
The main objective of the present work was to study the effect of cryopreservation of European eel sperm both on the sperm viability and the spermatozoa head morphology. Spermatozoa morphology was evaluated with computer-assisted morphology analysis after collection in fresh samples, after adding the freezing medium containing dimethyl sulfoxide as cryoprotectant and, finally, after the cryopreservation process and thawing. Cell viability was assessed, in both fresh and thawed samples, by Hoechst 33258 staining. Computer-assisted sperm analysis (CASA) was used to determine the percentage of motile cells and to measure motility parameters in sperm samples. A significant decrease of head perimeter (12.56%) and area (17.90%) was detected from spermatozoa in fresh to thawed samples, indicating that cells do not recover the original size after the cryopreservation process. CASA was used to measure the percentage of motile cells (51.9%) and spermatozoa motility parameters such as curvilinear, straight line and angular path velocities, as well as beating cross frequency. This technique was employed in the fresh sperm samples but proteins present at the freezing medium (L-alpha-phosphatidylcholine) made impossible to use this last technique in thawed samples. When sperm viability was assessed by Hoechst staining, a significant decrease of approximately 15% (73.10 vs 58.26%) of alive spermatozoa was registered from fresh to thawed samples. The percentage of motile cells measured by CASA in fresh samples (51.9%) was lower than the percentage of alive cells determined by Hoechst stainning, suggesting the existence of different batches of spermatozoa in different stages of development, even during the eight to tenth weeks of treatment, when the highest sperm quality was found. 相似文献
36.
37.
Field comparison of the interferon-gamma assay and the intradermal tuberculin test for the diagnosis of bovine tuberculosis 总被引：13，自引：0，他引：13
PR WOOD LA CORNER JS ROTHEL C. BALDOCK† SL JONES† DB COUSINS§ BS McCORMICK¶ BR FRANCIS§ J. CREEPER¶ NE TWEDDLE 《Australian veterinary journal》1991,68(9):286-290
An extensive field comparison of the gamma interferon (IFN-gamma) assay and the single intradermal tuberculin test for the diagnosis of bovine tuberculosis was conducted in Australia. The specificity of the IFN-gamma assay was determined by testing more than 6000 cattle from tuberculosis-free herds and varied from 96.2% to 98.1%, depending on the cut-off point chosen to define a positive reactor. For the sensitivity trial, cattle from herds being de-populated because of bovine tuberculosis were examined with both assays. The sensitivity of the IFN-gamma assay was shown to be significantly higher than the single intradermal tuberculin test and varied from 76.8% to 93.6% depending on the method of interpretation. A maximum overall sensitivity of 95.2% was obtained by testing with the IFN-gamma and the tuberculin test in parallel. The superior sensitivity of the IFN-gamma assay and the ability to adjust the sensitivity of the system depending on the task involved, will provide the Australian Tuberculosis Eradication Campaign with a valuable additional test to enable it to accomplish its goals. 相似文献
38.
39.
40.