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71.
Kyoung-Won Seo Ul-Soo Choi Bo-Kyoung Bae Mi-Sun Park Cheol-Yong Hwang Dae-Yong Kim Hwa-Young Youn 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(2):199-201
An 8-month old intact male Turkish Angora cat was referred to the Veterinary Medical Teaching Hospital (VMTH), Seoul National University, for an evaluation of anorexia and severe dyspnea. The thoracic radiographs revealed significant pleural effusion. A cytology evaluation of the pleural fluid strongly suggested a lymphoma containing variable sized lymphocytes with frequent mitotic figures and prominent nucleoli. The feline leukemia virus and feline immunodeficiency virus tests were negative. The cat was euthanized at his owner''s request and a necropsy was performed. A mass was detected on the mediastinum and lung lobes. A histopathology evaluation confirmed the mass to be a lymphoma. Immunohistochemistry revealed the mass to be CD3 positive. In conclusion, the cat was diagnosed as a T-cell mediastinal lymphoma. 相似文献
72.
To establish a molecular differentiation method for Salmonella enterica subsp. enterica, a hyper-variable region of RNA polymerase beta-subunit (rpoB) of S. enterica subsp. enterica (I), serotype Typhimurium, and Escherichia coli were investigated through comparison of nucleotide sequence of the region. The hyper-variable region was identified at 612-937 of the gene. After PCR amplification of the region in the 17 serotypes and two biotypes of serotype Gallinarum of S. enterica subsp. enterica (I), the nucleotide sequences of the region were determined and compared. All serotypes were distantly related to E. coli with 82.8-84.7% identities in nucleotide sequence while showing 96.6-100% identities with each other. According to the phylogenetic analysis based on the sequenced region with the neighbor-joining method, relatedness of biotype Gallinarum to serotype Enteritidis and biotype Pullorum was determined. Biotype Gallinarum was more closely related to serotype Enteritidis than biotype Pullorum. These results suggested that the 612-937 variable region of rpoB might be useful for molecular evolutionary analysis of serotypes of S. enterica subsp. enterica (I). 相似文献
73.
Yoo HS Lee BJ Chang BS Lee YS Park BK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(1):67-72
A field trial was conducted to evaluate effect of enrofloxacin-Na against pathogens related to the respiratory and alimentary diseases in eighty suckling piglets (6-7 days old) and eighty weanling piglets (5-6 weeks old). Respective twenty of the suckling and weanling piglets were assigned to each of 4 experimental groups; control (non-treated), clinical injection dose (CID), 2x clinical injection dose (2CID). and premix. A 0.05 ml (2.5 mg) of enrofloxacin-Na injection (5% solution, 1 ml) per kg body weight of piglets as CID was injected intramuscularly for 3 days and the clinical signs were observed for 9 days. The premix (150 ppm) of enrofloxacin-Na was administered with feed for 7 days ad libitum and the clinical signs were observed for 13 days. The enrofloxacin-Na-treated piglets showed a higher increase in body weight and a lower feed per gain than the control piglets. In addition, the treatment of enrofloxacin-Na, regardless of the route of administration, decreased the incidence rate of diarrhea in suckling piglets and respiratory symptoms in weanling piglets. The isolation index of E. coli and Cl. perfringens during the treatment periods was also lowered by the enrofloxacin-Na treatment in both suckling and weanling piglets. The antibiotics was also evaluated as safe locally and whole bodily as treated by injection or feeding. These results indicate that the newly developed antibiotics, enrofloxacin-Na, is very useful for the prevention and therapy of swine diseases in the pig industry. 相似文献
74.
Hwang IK Lee HY Yoo KY Seong NS Chung HG Kim JH Lee HJ Lee WH Kang TC Won MH 《Anatomia, histologia, embryologia》2004,33(4):220-224
P2X receptors play a role in the transduction of sensory signals like pain. Few studies have been undertaken on altered P2X(3) receptor (P2X3) expression in sensory neurones after peripheral nerve injury. In the present study, we investigated chronological alterations in P2X3 immunoreactivity and its protein content in the trigeminal ganglion after ischaemic insult in the Mongolian gerbil. In the sham-operated group, P2X3-immunoreactive neurones were found abundantly in small- and medium-sized neurones. From 1 day after ischaemic insult, the number of P2X3-immunoreactive neurones decreased significantly. At 5 days after ischaemic insult, P2X3 immunoreactivity was observed in few neurones, but its immunoreactivity was weak. However, the number of cresyl violet-positive neurones was unchanged throughout this period in all groups. These results suggest that transient trigeminal ganglion ischaemia may provoke a decrease of P2X3 expression and its protein content, and that this down-regulation of P2X3 may be related to the altered pain and thermal sensation without being associated with a transient ischaemic insult. 相似文献
75.
Xenotransplantation and the potential risk of xenogeneic transmission of porcine viruses. 总被引:1,自引:0,他引:1 下载免费PDF全文
The clinical success of allotransplantation and the shortage of donor organs have led to a proposal for the use of animal organs as alternative therapeutic materials for humans. In that regard, swine are preferable to non-human primates as a source of donor organs. While applications for clinical trials for xenotransplantation have not yet been received in Canada, several trials have already been authorized in the United States. A major concern, however, is the potential for xenogeneic transmission of viruses from animals to humans via organ, tissue, or cellular transplantation or via ex vivo exposure of humans to porcine biologic materials. Xenotransplantation allows viruses to bypass the normal immunological defense mechanisms of the recipient. Furthermore, the use of immunosuppressive drugs following transplantation may facilitate the xenogeneic transmission of zoonotic agents. Of porcine viruses, swine hepatitis E virus does not cause any clinical symptoms in the natural host but is a likely zoonotic agent that can infect humans and cause hepatitis. Porcine circovirus type 1 is prevalent in swine populations with no known association with clinical disease, while circovirus type 2 causes post-weaning multi-systemic wasting syndrome. Porcine endogenous retrovirus is integrated into the host chromosomes while porcine cytomegalovirus undergoes latent infection. Two additional porcine herpesviruses have recently been identified in swine and have been named porcine lymphotrophic herpesviruses. These herpesviruses can potentially become reactivated in human recipients after xenotransplantation. All in all, there are a number of viruses in swine that are of primary concern to screen and eliminate from xenotransplantation protocols. Epidemiology and the current knowledge on xenogeneic risk of these viruses are discussed. 相似文献
76.
Lee JY Yoo BC Rojas MR Gomez-Ospina N Staehelin LA Lucas WJ 《Science (New York, N.Y.)》2003,299(5605):392-396
In plants, cell-to-cell communication is mediated by plasmodesmata and involves the trafficking of non-cell-autonomous proteins (NCAPs). A component in this pathway, Nicotiana tabacum NON-CELL-AUTONOMOUS PATHWAY PROTEIN1 (NtNCAPP1), was affinity purified and cloned. Protein overlay assays and in vivo studies showed that NtNCAPP1 is located on the endoplasmic reticulum at the cell periphery and displays specificity in its interaction with NCAPs. Deletion of the NtNCAPP1 amino-terminal transmembrane domain produced a dominant-negative mutant that blocked the trafficking of specific NCAPs. Transgenic tobacco plants expressing this mutant form of NtNCAPP1 and plants in which the NtNCAPP1 gene was silenced were compromised in their ability to regulate leaf and floral development. These results support a model in which NCAP delivery to plasmodesmata is both selective and regulated. 相似文献
77.
Yoo SH Lee KH Lee JS Cha J Park CS Lee HG 《Journal of agricultural and food chemistry》2005,53(16):6235-6239
Physicochemical characteristics and biological activities of Sphingomonas gellan (S-gellan) were investigated. The S-gellan weight fractions of Glc and GlcUA were 0.45 and 0.25, respectively, and the molar ratio of Glc:Rha:GlcUA was approximately 4:2:3. The S-gellan was chemically derivatized with diethylaminoethyl chloride-HCl (DEAE-HCl), and the resulting modified S-gellan contained both positive and negative charges. The elemental and IR analyses were conducted to confirm the successful incorporation of DEAE groups into S-gellan. A large increase in nitrogen fraction was observed from the derivatized S-gellan by elemental analysis. The IR absorption bands induced by C-H, C-N, and C-O-C stretching were noticeable at 2950, 1310-1380, and 1000-1150 cm(-1), respectively, resulting from the DEAE substitution. The characteristic CH3 and CH2 peaks originated from the DEAE group were detected in the 1H NMR spectrum of the derivatized S-gellan as well. The solubility of native S-gellan was improved almost twice from 40% to 75% after DEAE derivatization, while water holding capacity (WHC) drastically decreased from 10026% to 245%. Oil binding capacity (OBC) of S-gellan also significantly dropped from 1528% to 331% after the derivatization. The bile acid binding capacity of S-gellan was indirectly determined by measuring the holding capability of cholic acid inside the dialysis membrane (MWCO 12,000-14,000 Da). Once S-gellan was DEAE derivatized, there was substantial increase in the cholic acid retardation index (CRI). Up to 9 h of dialysis, the derivatized S-gellan released 29.3% less of cholic acid compared to the control group that did not contain S-gellan. From these results of the improved water solubility and stronger bile acid binding capacity, it would be suggested that the DEAE-derivatized S-gellan has more advantages than gellan itself for functional food applications. 相似文献
78.
79.
Gwangyeol Yoo Seunghyung Lee Young Chul Kim Okorie E. Okorie Gun Jun Park Yong Ok Han Se-Min Choi Ju-Chan Kang Mihai Sun Sungchul C. Bai 《Journal of the World Aquaculture Society》2007,38(1):138-145
Abstract.— The present study was conducted to investigate the effects of dietary supplementation of β‐1,3 glucan and a laboratory developed feed stimulant, BAISM, as feed additives for juvenile olive flounder, Paralichthys olivaceus. Eight experimental diets were formulated to be isonitrogenous and isocaloric and to contain 50.0% crude protein and 16.4 kJ of available energy/g with or without dietary β‐1,3 glucan and BAISM supplementation. β‐1,3 glucan (G) and BAISM (B) were provided at 0% in the control diet (G0B0) and at 0.05% G + 0.45% B (G0.05B0.45), 0.05% G + 0.95% B (G0.05B0.95), 0.1% G + 0.90% B (G0.1B0.9), 0.10% G + 1.90% B (G0.1B1.9), 0.15% G + 1.35% B (G0.15B1.35), 0.15% G + 2.85% B (G0.15B2.85), and 0.30% G + 2.70% B (G0.3B2.7) in experimental diets. After the feeding trial, fish fed G0.1B0.9, G0.1B1.9, and G0.15B1.35 diets had higher percent weight gain (WG), feed efficiency ratio (FER), specific growth rate (SGR), protein efficiency ratio (PER), and condition factor (CF) than those fed G0B0, G0.05B0.45, G0.05B0.95, G0.15B2.85, and G0.3B2.7 diets (P < 0.05); however, there was no significant differences among fish fed G0.1B0.9, G0.1B1.9, and G0.15B1.35 diets. Fish fed G0.1B0.9 and G0.1B1.9 diets had higher chemiluminescent responses (CL) than those fed the other diets (P < 0.05). Lysozyme activity of fish fed G0.1B0.9 diet was significantly higher than that of fish fed the other diets (P < 0.05). These results indicated that the optimum dietary supplementation level of β‐1,3 glucan and BAISM could be approximately 0.10% β‐1,3 glucan + 0.90% BAISM (G0.1B0.9) of diet based on WG, FER, SGR, PER, CF, CL, and lysozyme activity in juvenile olive flounder, P. olivaceus. 相似文献
80.
Mihai Sun Young Chul Kim Okorie Eme Okorie Sukumar Devnath Gwangyeol Yoo Seunghyung Lee Yong Kil Jo Sungchul C. Bai 《Journal of the World Aquaculture Society》2007,38(4):543-549
A 10‐wk feeding trial was conducted to evaluate the potential use of fermented fisheries by‐products and soybean curd residues mixture (FFSM) as a partial replacement for fish meal (FM) in the diet of juvenile olive flounder, Paralichthys olivaceus. Five experimental diets were formulated with FFSM replacing 0, 15, 30, 45, and 60% of the FM protein (FFSM0, FFSM15, FFSM30, FFSM45, and FFSM60, respectively). Juvenile olive flounder averaging 5.99 ± 0.08 g (mean ± SD) were randomly distributed into aquaria at 15 fish/aquarium, with three replicate aquaria for each experimental diet. Weight gain (WG) of fish fed FFSM0, FFSM15, and FFSM30 was significantly higher than that of fish fed FFSM45 and FFSM60 (P < 0.05). Also, WG of fish fed FFSM45 was significantly higher than that of fish fed FFSM60 (P < 0.05). There were no significant differences in WG among fish fed FFSM0, FFSM15, and FFSM30 (P > 0.05). Specific growth rate (SGR) of fish fed FFSM15 was significantly higher than that of fish fed FFSM45 and FFSM60 (P < 0.05). Also, SGR of fish fed FFSM0, FFSM15, FFSM30, and FFSM45 was significantly higher than that of fish fed FFSM60 (P < 0.05). There were no significant differences in SGR among fish fed FFSM0, FFSM15, and FFSM30 and among those fed FFSM0, FFSM30, and FFSM45 (P > 0.05). Feed efficiency (FE) and protein efficiency ratio (PER) of fish fed FFSM60 were significantly lower than those of fish fed FFSM0, FFSM15, FFSM30, and FFSM45 (P < 0.05); however, there were no significant differences in FE and PER among fish fed FFSM0, FFSM15, FFSM30, and FFSM45. Hepatosomatic index of fish fed FFSM0, FFSM15, and FFSM30 was significantly higher than that of fish fed FFSM60 (P < 0.05); however, there were no significant differences among fish fed FFSM0, FFSM15, FFSM30, and FFSM45 and among those fed FFSM45 and FFSM60. No significant differences were observed in condition factor and survival rate among all dietary groups tested. The whole‐body proximate composition averaged 75.0 (% dry matter basis [DM]), 8.66 (% DM), 16.38 (% DM), and 76.1%, for crude protein, crude lipid, ash, and moisture, respectively. Based on growth performance, the FFSM could replace up to 30% FM protein by the ANOVA test; however, broken‐line model analysis indicated 28.7% as an optimum replacement level in juvenile olive flounder diets. 相似文献