银纹夜蛾人工饲养技术研究   总被引：2，自引：0，他引：2  

刘军  刘复生 《昆虫天敌》1994,16(4):170-174

本文在研究了各种环境因子对银纹夜蛾实验种群影响的基础上，进一步研究了食量，低温保种及饲养管理技术，设计了一种便于收集卵的新型产卵笼，并总结了人工饲养银纹夜蛾的技术要点。  相似文献   

苹果枝梢化学成分与对苹果绵蚜抗性的比较     

林三冬  冯凯  陈钢  邓家祺 《植物保护学报》1994,21(1):46-46

果树对苹果绵蚜的抗性与多种因素有关。其中营养成分α-氨基氮的含量,以及毒性成分多酚(φ)的含量尤为重要。  相似文献   

Influence of application methods on the degradation of permethrin in laboratory,soil aerobic metabolism studies     

Michael W. Skidmore  David Kirkpatrick  David Shaw 《Pest management science》1994,42(2):101-107

The effects of application rate, volume, solvent and soil moisture content on the kinetics of mineralization and degradation, of [¹⁴C] permethrin have been studied in a sandy loam soil under standard laboratory conditions. During the incubation period, up to 32 days, the temperature and moisture level of the soil were controlled. Apart from the effects of application rate, which have been widely reported, application volume had the most significant effect on mineralization rate and T_1/2. [¹⁴C]Permethrin, at a level of a 1 mg kg^?1 in the soil, applied in 100 μl of methanol, resulted in the evolution of 14% of the applied radiochemical as [¹⁴C] carbon dioxide over 30 days. The same level applied in 1000 μl mineralized at a faster rate, with 30% [¹⁴C]carbon dioxide evolved over 30 days. The test chemical applied to soil in methanol mineralized at a significantly faster rate than a similar concentration applied in ethanol. There was no significant difference when comparing applications made using acetonitrile with those using methanol or ethanol. The addition of formulation ingredients resulted in little or no variation in mineralisation rate compared to an equivalent application volume of methanol/water.  相似文献   

小地老虎抗寒能力的研究     

王荫长  陈长琨  尤子平 《植物保护学报》1987,14(1):9-14

小地老虎各虫态的过冷却点,卵期为-9.5℃,一至六龄幼虫分别为-8.8、-4.1、-3.6、-3.0、-2.3与-1.2℃,蛹期为-6.6℃,成虫期为-4.3℃。越冬幼虫在-5—0℃下1天内即死亡,2—4℃间能存活2个月,低温锻炼不能提高幼虫的抗寒力。六龄幼虫在4℃时停食,消化道内酯酶活性下降。成虫在越冬期间,若无补充营养,只能存活2个月。  相似文献   

制备植物病毒、细菌抗血清的连续采血法研究和应用     

张作芳  张成良  宋淑敏  胡伟贞  陈京  阙晓枫  陆家珏 《植物保护学报》1987,14(3):173-176

用植物病毒、细菌抗源(加福氏完全佐剂)免疫家兔,从末次免疫后7天开始,1—5个月内进行耳静脉连续采血,1周2次,能获得比心脏采血多15倍的抗血清。耳静脉抗血清用于酶联免疫吸附试验、免疫电镜、反向间接血凝、乳胶凝集试验、免疫双扩散、对流免疫电泳技术检测抗原,均得到很好的结果。  相似文献   

苹果树腐烂病(Valsa malimiyabe et Yamada)侵染时期研究   总被引：3，自引：0，他引：3  

陈策  李美娜  史秀琴  王金有 《植物病理学报》1987,17(2):65-68

 本文报导了苹果树腐烂病侵染时期的研究结果.明确了我国辽宁西部的兴城,从3月至11月均能发生侵染,从3月下旬至5月中旬侵染最为集中.就树皮腐生真菌对腐烂病菌侵染的影响做了初步探索.  相似文献   

Cryopreservation of gander semen   总被引：2，自引：0，他引：2  

Tai JJ  Chen JC  Wu KC  Wang SD  Tai C 《British poultry science》2001,42(3):384-388

1. The effect of dimethylacetamide (DMA) and dimethyl sulphoxide (DMSO) on the cryopreservation of gander semen were investigated. An improved survival rate of spermatozoa after freeze-thawing was obtained when semen was frozen by a fast-freezing procedure on dry ice with 9% DMA as the cryoprotectant. 2. Gander semen, which was frozen during mid season, was tested for fertilising ability in different times of the season. The percentage of fertility during d 3 to d 9 after 2 consecutive inseminations was 68% to 95%, depending on the date of artificial insemination.  相似文献   

Effects of chromium nicotinate on performance, carcase characteristics and blood chemistry of growing turkeys     

Chen KL  Lu JJ  Lien TF  Chiou PW 《British poultry science》2001,42(3):399-404

1. The objective of this trial was to study the effects of chromium nicotinate (Cr) supplementation on the performance, carcase characteristics and blood constituents of 9 to 22 week-old male turkeys. 2. Fifty-four 8-week-old male turkeys weighing 2.9 +/- 0.28 kg were allocated at random into 9 groups with 3 dietary treatments and 3 replicates. The dietary treatment was different dietary concentrations of chromium in the basal diet, to provide 0 (control), 1 or 3 mg/kg. The feeding trial lasted for 14 weeks. 3. Results showed that 1 mg/kg chromium supplementation significantly improved weight gain and food intake at 9 to 18 weeks of age but did not significantly influence performance at 19 to 22 week of age. The breast and thigh muscle were significantly increased in birds receiving 1 mg/kg chromium supplementation but were decreased by 3 mg/kg of supplementation. 4. Dietary chromium supplementation did not significantly influence other serum constituents, including insulin, HDL, VLDL+LDL, HDL-C, VLDL-C+LDL-C, total protein, albumin, and gamma-globulin at 18 and 22 weeks of age. 5. The serum triacylglycerol (TG) and uric acid contents were significantly increased, while glycerol and alpha-globulin were significantly reduced by 3 mg/kg chromium supplementation. However, 1 mg/kg chromium supplementation significantly reduced serum cholesterol and glycerol at 18-weeks old. At 22 weeks of age, 1 mg/kg chromium supplementation significantly increased serum glucose and decreased the uric acid concentration whereas 3 mg/kg chromium supplementation significantly increased the creatinine concentration and decreased beta-globulin concentration.  相似文献   

Canine model and genomic structural organization of glycogen storage disease type Ia (GSD Ia)     

Kishnani PS  Faulkner E  VanCamp S  Jackson M  Brown T  Boney A  Koeberl D  Chen YT 《Veterinary pathology》2001,38(1):83-91

A canine model of glycogen storage disease Ia (GSD Ia), similar clinically, biochemically, and pathologically to the human disease, was established by crossbreeding Maltese and Beagle dogs carrying a mutated, defective glucose-6-phosphatase (G-6-Pase) gene. Ten puppies were born in three litters from these crossbreedings. Six were homozygous for the previously described M121I GSD Ia mutation. Of these six affecteds, two were stillborn, and one died at 2, 32, and 60 days of life, respectively (puppies A, B, C, D, E), while one is alive at age 15 months (puppy F). Affected puppies exhibited tremors, weakness, and neurologic signs when hypoglycemic. They had postnatal growth retardation and progressive hepatomegaly. Biochemical abnormalities included fasting hypoglycemia, hyperlactacidemia, hypercholesterolemia, hypertriglyceridemia, and hyperuricemia. Microscopic examination of tissues from affected puppies showed diffuse, marked hepatocellular vacuolation, with distended clear hepatocytes and central to marginally located rounded nuclei. In the kidneys of puppies D and E, there was segmental glomerular sclerosis and vacuolation of proximal convoluted tubular epithelium. Biochemical analysis revealed increased liver glycogen content and isolated markedly reduced G-6-Pase enzyme activity in liver and kidney. The canine G-6-Pase gene was characterized by screening a canine genomic library. It spans approximately 11.8 kb and consists of five exons with >90% amino acid sequence homology to the derived human sequence. The first 1.5 kb of the 5' region was sequenced and contains several putative response element motifs homologous to the human 5' region. Establishment of this canine colony of GSD Ia that closely resembles human disease and isolation of the canine genomic gene provides an excellent model for studying pathophysiology and long-term complications and an opportunity to develop novel therapeutic approaches such as drug and gene therapy.  相似文献   

夏秋季品种“华夏二号”（研·丰×7N·湘F）的选育（初报）     

陈列辉  廖琼香 《广东蚕业》2001,35(4):22-27

采用NaF水溶液进行累代或隔代添食的方法,选择"研137"、"丰一"、"7N"、"湘F"四个元母种,组配成四元杂交组合"研·丰×7N·湘F"进行室内及农村试养,并参加广东省新蚕品种品比鉴定.结果表明,该杂交组合耐氟性能强,综合经济性状优于现行生产品种"9·芙×7·湘".  相似文献