Oxidative damage mediated by herbicides on yeast cells     

Braconi D  Possenti S  Laschi M  Geminiani M  Lusini P  Bernardini G  Santucci A 《Journal of agricultural and food chemistry》2008,56(10):3836-3845

Agricultural herbicides are among the most commonly used pesticides worldwide, posing serious concerns for both humans, exposed to these chemicals through many routes, and the environment. To clarify the effects of three herbicides as commercial formulations (namely, Pointer, Silglif, and Proper Energy), parameters related to oxidative issues were investigated on an autochthonous wine yeast strain. It was demonstrated that herbicides were able to affect the enzymatic activities of catalase and superoxide dismutase, as well as to induce carbonylation and thiol oxidation as post-translational modifications of proteins. Saccharomyces cerevisiae is an optimal model system to study responses to xenobiotics and oxidative stress. Thus, the results obtained could further the understanding of mechanisms underlying the toxicity of herbicides.  相似文献   

Multiple feather follicle cysts in a Moroseta hen (Gallus gallus)     

Mutinelli F  Corro' M  Catania S  Melchiotti E 《Avian diseases》2008,52(2):345-347

An 8-month-old white feathered, black skinned Moroseta hen was presented for examination because of numerous 2 mm- to 30 mm-diameter irregularly shaped, hard nodules in the skin of the head, wings, back, and abdomen. The nodules were confined to the skin and did not involve subcutaneous tissues. Nodules consisted of dilated feather follicles packed with a caseous tan-to-pale-yellow material admixed with feather remnants. Histologically, affected feather follicles were markedly dilated and filled with laminated keratin debris. Necrosis of the epidermis and perifollicular lymphocyte infiltration was also present. Bacteriologic investigation of internal organs was negative, while secondary bacteria, Proteus spp. and Bacillus spp., were isolated from skin nodules. A concomitant lice infestation of Menopon spp., as well as leg mange caused by Cnemidocoptes spp., were also present. These bacterial isolates and parasites were not related to the disease condition. The condition observed was differentiated from benign feather follicle tumors, and a diagnosis of multiple feather follicle cysts was made. In addition, a breed predisposition was hypothesized.  相似文献   

Lymphocyte beta -adrenoceptor downregulation in great danes with occult dilated cardiomyopathy (DCM) and with DCM and heart failure.   总被引：2，自引：0，他引：2  

M Borgarelli  P Badino  L Bergamasco  C Bussadori  R Odore  G Re  A Tarducci  U Dotta 《Veterinary journal (London, England : 1997)》1999,158(2):128-134

The importance of the adrenergic nervous system in supporting the failing heart has long been known. The adrenergic drive on cardiac structure and function has however some adverse effects, which include myocardial beta-adrenoceptor (beta-AR) downregulation and decreased beta-adrenergic responsiveness to cathecolamines. In dog lymphocytes, beta(1)-AR and beta(2)-AR populations are almost equally represented (with a slight prevalence of beta(2)) and a significant correlation between cardiac and lymphocytic adrenoceptors has been found. The aim of the present study was to investigate possible differences between the concentration of lymphocytic beta-AR in healthy dogs, dogs with dilated cardiomyopathy (DCM) and dogs with occult DCM. Three groups of great danes were considered: a control group (n =10), dogs with DCM (n =9) and dogs with occult DCM (n =4). Lymphocytic beta-AR populations were determined in all dogs. A substantial and significant decrease (P<0.05) in total-AR, beta(1)-AR and beta(2)-AR concentrations in the lymphocytes of dogs with symptomatic DCM and occult DCM compared to the control group was found. Although the mean value of the lymphocyte beta(1)-AR number in the asymptomatic group was double compared to the DCM group, this difference was not statistically significant. We conclude that in dogs beta-AR downregulation occurs early in the course of dilated cardiomyopathy. This finding may suggest the value of early use of a beta-blocker in the therapeutic regimen. Moreover, the continuous monitoring of lymphocytic beta-AR may represent a useful tool for the development of a more effective individual therapy.  相似文献   

Physiological responses of pink abalone Haliotis corrugata (Gray, 1828) exposed to different combinations of temperature and salinity     

Zarina Medina Romo  Ana Denisse Re  Fernando Díaz  Alfredo Mena 《Aquaculture Research》2010,41(7):953-960

Physiological responses of pink abalone Haliotis corrugata were determined under different temperature and salinity conditions. Oxygen consumption rate was not affected by temperature and salinity. Ammonium excretion of pink abalone was inversely related to salinity. The O:N ratio indicated that abalone maintained in lower salinities had an interval of 4.9–7.7, which is indicative of a protein‐dominated metabolism, whereas the O:N in 35‰ was 28.8–35.5 for both temperatures, suggesting that carbohydrates were used as energy substrate. Haemolymph osmolality of abalone exposed to 20 and 24 °C was slightly hyperiso‐osmoconformic in salinity ranges of 20–35‰. The results of this study suggested that for optimized culture, pink abalone should be cultivated at 24 °C at a salinity of 35‰.  相似文献   

Fluctuating salinity effect on Sphoeroides annulatus (Jenyns 1842) physiological responses     

Javier Prez‐Robles  Ana Denisse Re‐Araujo  Maricela Cortez‐García  Fernando Díaz  Leonardo Ibarra‐Castro  Ivone Giffard‐Mena 《Aquaculture Research》2019,50(1):198-208

Euryhaline fish, such as the Bullseye puffer Sphoeroides annulatus (Jenyns 1842), experience sudden salinity changes in their natural environment, which is more common than the exception, so they must adapt to survive and cope with extreme salt conditions. Therefore, Bullseye puffer juveniles were exposed to short‐term stress (39 hr) by fluctuating salinity conditions (41, 35, 29, 23, 17, 11, 5, 11, 17, 23, 29, 35, 41 psu) with a 3‐hr interval between each point at 26 ± 1ºC in a respirometer chamber and acclimation reservoirs. Responses to oxygen consumption rate (OCR: 23–35 mg O₂ h^–1 kg^–1), ammonium excretion rate (AER: 1–1.85 mg NH4⁺ h^?1 kg^?1), oxygen‐nitrogen atomic ratio (O:N 17–30), osmoregulatory pattern (blood osmotic pressure from 342.4 to 332.8 mmol/kg) and changes in expression levels of Na⁺/K⁺‐ATPase in the gills (higher values at higher salinities) were measured. Although some signs of stress were detected below the iso‐osmotic point (11.4 psu), the puffer fish is a strong euryhaline fish that survives under these conditions. Nonetheless, it could recover when salinity returned to the initial acclimation point because Sphoeroides annulatus is able to live in a wide range of environments with wide natural salinity fluctuations; thus, a common practice in aquaculture has been to expose fish to low salinity for several reasons discussed in this study. This capacity reveals its high plasticity to saline adaptation from 41 to 5 psu an up from 5 to 41 psu, all in less than 2 days.  相似文献   

Cloning,characterization, and molecular expression of gonadotropin receptors in European hake (<Emphasis Type="Italic">Merluccius merluccius</Emphasis>), a multiple-spawning species     

Michela Candelma  Luisa Dalla Valle  Sabrina Colella  Alberto Santojanni  Oliana Carnevali 《Fish physiology and biochemistry》2018,44(3):895-910

Teleosts have many spawning strategies and the hormonal control of gametogenesis is not well defined among the species or even, between sexes. To increase the knowledge of gonadotropin hormones, we studied the trend by gene expression of gonadotropin receptors in the follicles and testis at different maturity stages in the European hake (Merluccius merluccius), a multiple-spawning species. With this aim, fshr and lhr were sequenced, characterized, and their gene expression was quantified in oocytes and in testes at different maturity stages. The deduced amino acid sequences were used to phylogenetic studies and evidenced that both receptors are phylogenetically closed to other gadoid species. The gene expression of both receptors was poorly expressed in primary follicles, increased in vitellogenic follicles and to later decrease in hydrated oocytes. In testis, highest levels of lhr were detected during spermiation, while levels of fshr were constant. For the first time, a histological analysis was performed in European hake testes showing an unrestricted lobular testis. To better elucidate the mechanisms involved in the oogenesis of the European hake, the expression of estrogen receptor and cyp19a was also investigated displaying high levels in all classes of follicles. All these data allow to increase the knowledge on reproductive physiology of an important socioeconomical species and it seeks to shed more light on the role of the receptors here studied during gametogenesis of multiple-spawning fish.  相似文献   

Massive wood material for sustainable building design: the Massiv–Holz–Mauer wall system     

Silvia Santi  Francesca Pierobon  Giulia Corradini  Raffaele Cavalli  Michela Zanetti 《Journal of Wood Science》2016,62(5):416-428

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Protective effect of the AT137RQ and ARQK176 PrP alleles against classical scrapie in Sarda breed sheep     

Gabriele Vaccari  Gaia Scavia  Marcello Sala  Gianmario Cosseddu  Barbara Chiappini  Michela Conte  Elena Esposito  Raniero Lorenzetti  Gabriella Perfetti  Paola Marconi  Francesco Scholl  Katia Barbaro  Antonino Bella  Romolo Nonno    Umberto Agrimi 《Veterinary research》2009,40(3)

The susceptibility of sheep to scrapie is under the control of the host’s prion protein (PrP) gene and is also influenced by the strain of the agent. PrP polymorphisms at codons 136 (A/V), 154 (R/H) and 171 (Q/R/H) are the main determinants of susceptibility/resistance of sheep to classical scrapie. They are combined in four main variants of the wild-type ARQ allele: VRQ, AHQ, ARH and ARR. Breeding programmes have been undertaken on this basis in the European Union and the USA to increase the frequency of the resistant ARR allele in sheep populations. Herein, we report the results of a multi-flock study showing the protective effect of polymorphisms other than those at codons 136, 154 and 171 in Sarda breed sheep. All ARQ/ARQ affected sheep (n = 154) and 378 negative ARQ/ARQ controls from four scrapie outbreaks were submitted to sequencing of the PrP gene. The distribution of variations other than those at the standard three codons, between scrapie cases and negative controls, was statistically different in all flocks. In particular, the AT₁₃₇RQ and ARQK₁₇₆ alleles showed a clear protective effect. This is the first study demonstrating a protective influence of alleles other than ARR under field conditions. If further investigations in other sheep breeds and with other scrapie sources confirm these findings, the availability of various protective alleles in breeding programmes of sheep for scrapie resistance could be useful in breeds with a low frequency of the ARR allele and would allow maintaining a wider variability of the PrP gene.  相似文献   

Polyunsaturated Fatty Acids of Marine Macroalgae: Potential for Nutritional and Pharmaceutical Applications     

Hugo Pereira  Luísa Barreira  Filipe Figueiredo  Luísa Custódio  Catarina Vizetto-Duarte  Cristina Polo  Eva Re?ek  Aschwin Engelen  Jo?o Varela 《Marine drugs》2012,10(9):1920-1935

As mammals are unable to synthesize essential polyunsaturated fatty acids (PUFA), these compounds need to be taken in through diet. Nowadays, obtaining essential PUFA in diet is becoming increasingly difficult; therefore this work investigated the suitability of using macroalgae as novel dietary sources of PUFA. Hence, 17 macroalgal species from three different phyla (Chlorophyta, Phaeophyta and Rhodophyta) were analyzed and their fatty acid methyl esters (FAME) profile was assessed. Each phylum presented a characteristic fatty acid signature as evidenced by clustering of PUFA profiles of algae belonging to the same phylum in a Principal Components Analysis. The major PUFA detected in all phyla were C₁₈ and C₂₀, namely linoleic, arachidonic and eicosapentaenoic acids. The obtained data showed that rhodophytes and phaeophytes have higher concentrations of PUFA, particularly from the n-3 series, thereby being a better source of these compounds. Moreover, rhodophytes and phaeophytes presented “healthier” ∑n-6/∑n-3 and PUFA/saturated fatty acid ratios than chlorophytes. Ulva was an exception within the Chlorophyta, as it presented high concentrations of n-3 PUFA, α-linolenic acid in particular. In conclusion, macroalgae can be considered as a potential source for large-scale production of essential PUFA with wide applications in the nutraceutical and pharmacological industries.  相似文献   

Performance of common buckwheat (Fagopyrum esculentum M.) in response to row spacing under ridge and furrow cropping systems in a semiarid region of China     

Ying Jiang  Xinxin Wang  Zhaohai Zeng  Jinjing Han  Michela Schiavon  Chao Tang 《Archives of Agronomy and Soil Science》2018,64(13):1807-1817

Ridge and furrow systems as well as the wide-narrow row spacing are recognized as good management practices in crop production. To obtain some available information concerning the suitable agricultural practices for buckwheat cultivation in arid and semi-arid area, a two-year field study was conducted to investigate the performance of common buckwheat grown under six cropping patterns: conventional flat single/double row planting (NS/ND); furrow single/double row planting (FS/FD); ridge single/double row planting (RS/RD); single row planting with equal row spacing and double row planting with wide-narrow row spacing practice. Plants grown under the FD system exhibited higher water use efficiency (WUE) and rainfall use efficiency (RUE) than plants cultivated with the NS system, increases with 13.7% and 21.9%, respectively, in 2014, while 9.8% and 14.0%, respectively, in 2015. Plants of the FD system also displayed the greatest leaf area index and canopy openness at growth stage of 40, 60, and 80 days after sowing, maximum biomass production (13.96 t ha^?1) and grain yield (3486.2 kg ha^?1) in 2014, and the least pronounced soil nitrate consumption as compared to plants from other tillage systems. We conclude that FD is the optimum planting pattern for common buckwheat cultivation in semi-arid and arid environments.  相似文献