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991.
Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue 总被引:23,自引:0,他引:23
In an effort to optimize tissue culture responses of turf‐type tall fescue for genetic transformation, the effects of five culture medium supplements on tissue culture responses were investigated with immature embryos and mature seeds of an elite cultivar, ‘Coronado’, as explant tissues. For both explants, calli induced on 6‐benzylaminopurine (BAP)‐containing medium had significantly improved regeneration ability. The optimal concentration of BAP for the induction of regenerable callus from mature seeds was 0.1 mg/l. Thidiazuron also improved callus regeneration frequency of mature seeds. Casein hydrolysate, L‐proline and myo‐inositol improved callus induction of immature embryos but not mature seeds. They did not improve callus regeneration frequencies with either explant tissue. By simply slicing the mature seeds into two halves longitudinally, the callus induction frequencies, as well as the corresponding overall plant regeneration frequencies, were increased approximately three‐ to six‐fold in all three combinations of 2,4‐dichlorophenoxyacetic acid and BAP in callus induction media. 相似文献
992.
Cotton fibre mutants that were fuzzless and/or lintless were crossed with each other and a normal genotype (fuzzy, linted) to produce F2 and BC1 generations. F2 segregation ratios from the cross of fuzzless‐lintless × fuzzy‐linted, for fuzzy‐linted, fuzzless‐linted and fuzzless‐lintless were 45 : 15 : 4. From the cross of fuzzless‐lintless × fuzzy‐linted, the F2 segregation ratios were 9 : 39 : 16 whereas the BC1F1 segregation ratios from the F1 backcrossed to fuzzless‐lintless were 1 : 3 : 4. These data suggest that the presence or absence of lint and fuzz are controlled by the interaction of four gene loci on non‐homologous chromosomes. We designate these loci as N1, N2, Li3 and Li4, where N1 N1 confers the presence of fuzzy, N2N2 confers inhibition of fuzzy initiation and development, and duplicate gene pairs, Li3Li3 and Li4Li4, determine the presence of lint. Homozygosity for li3li3 and li4li4 might also inhibit fuzz from development. In other words, they were recessive epistatic to fuzz genes. 相似文献
993.
The gene Pi15 for resistance of rice to Magnaporthe grisea was previously mapped to a ≈0.7-cM region on chromosome 9. To further define the chromosomal region of the Pi15 locus, a contig spanning the locus was constructed, in silico , through bioinformatics analysis using a reference sequence of the cultivar 'Nipponbare'. One simple sequence repeat marker adopted from the International Rice Microsatellite Initiative and six candidate resistance gene (CRG) markers, developed from gene annotation of the reference sequence of the contig, were used for linkage analysis in a mapping population consisting of 504 extremely susceptible F2 plants. The Pi15 locus was delimited to a ≈0.5-cM region flanked by the markers CRG5 and CRG2 and co-segregated with the markers BAPi15782 , CRG3 and CRG4, which was physically converted to a 44-kb interval. 相似文献
994.
The aluminium tolerance of 31 varieties within 11 species in six genera of the Cruciferae was determined by measuring root length under aluminium stress. Variance analysis indicated highly significant differences of aluminium tolerance among the species compared. Raphanus sativus (2n= 18, RR) had the highest aluminium tolerance, followed by Brassica carinata (2n= 34, BBCC), Brassica oleracea (2n= 18°C) and Brassica napus (2n= 38, AACC), which had the C genome. There might be more than one gene for aluminium tolerance in the R and C genomes. Those species such as Brassica campestris (2n= 20, AA), Brassica nigra (2n= 16, BB), Brassica juncea (2n= 36, AABB), Arabidopsis thaliana (2n= 10, arar), Sinapis alba (2n= 24, alal), Cheiranthus cheiri (2n= 12, chch) and Orychophragmus violaceus (2n= 24, OO) that did not include the R or C genome had lower aluminium tolerances. Transfer of aluminium tolerance from R. sativus into the cultivated brassicas seems possible by intergeneric hybridization and the production of addition, substitution or translocation lines. 相似文献
995.
L.?F.?García?del?MoralEmail author Y.?Rharrabti S.?Elhani V.?Martos C.?Royo 《Euphytica》2005,146(3):203-212
The components of grain yield are altered by adverse growing conditions as the effects of certain environmental factors on
crop growth and yield differ depending upon the developmental stages when these conditions occur. Path-coefficient analysis
was used to investigate the main processes influencing grain yield and its formation under Mediterranean conditions. Twenty-five
durum wheat genotypes, consisting of four Spanish commercial varieties and 21 inbred lines from the ICARDA durum wheat breeding
program, were grown during 1997 and 1998 under both rainfed and irrigated conditions in southern Spain. {P}ath-coefficient
analysis revealed that under favourable conditions grain yield depended in equal proportion on the three primary yield components
(i.e. spikes m−2, grains spike−1, and mean grain weight), whereas in the rainfed experiments, variations in grain yield were due mainly to spikes m−2 and to a lesser extent to grains spike−1. Compensatory effects were almost absent under irrigated treatments; however, under water shortage, spikes m−2 exerted a negative influence on grain spike−1 due mainly to a negative interrelationship between tiller production and apical development. These compensatory effects could
partially explain the restricted success in durum wheat breeding observed in water-limited environments of the Mediterranean
region. Under rainfed conditions the number of spikes m−2 depended mainly on the ability for tiller production, whereas in the irrigated experiments the final number of spikes was
determined mostly by tiller survival. 相似文献
996.
Evaluation of 'quick and dirty' DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.) 总被引:2,自引:0,他引:2
Six simple methods for extracting DNA from rice seedlings were evaluated for marker‐assisted selection (MAS). The assessment of each method was based on PCR amplification of SSR markers, DNA yield and purity, time and cost. Based on these criteria, two methods were selected as being superior to other methods. The best two methods included the standard method developed at the International Rice Research Institute (IRRI), which utilizes a sodium dodecyl sulfate extraction buffer followed by chloroform/isoamyl alcohol extraction and a previously published method using sodium hydroxide and Tris. These two methods produced nearly identical PCR amplification results. The sodium hydroxide method is considerably simpler, quicker and cheaper than the standard IRRI method, and may be particularly useful for many applications of MAS or high‐resolution mapping. This method was also adapted into an effective high throughput method utilizing 96‐well plates emphasizing its versatility. 相似文献
997.
Estimating the genetic relationship of hybrid bromegrass to smooth bromegrass and meadow bromegrass using RAPD markers 总被引:4,自引:0,他引:4
The two most widely grown bromegrass species in North America are smooth bromegrass, a hay type grass, and meadow bromegrass, a pasture type grass. Hybrid populations between these two species have been developed through hybridization and recurrent selection. The objective of this study was to determine the genetic relatedness of the hybrid bromegrass population S‐9073M to its parental populations using random amplified polymorphic DNA (RAPD) markers and to determine genetic variation within and between populations. Individuals from each of the three populations were genotyped at 43 polymorphic RAPD loci. One of the RAPD fragments was meadow bromegrass‐specific. Cluster analysis showed three groups representing the two parental populations and the hybrid population. An analysis of molecular variance (AMOVA), showed that the hybrid population had the highest within‐population variation, followed by meadow bromegrass and smooth bromegrass. The interpopulation genetic distance (phi‐statistic =Φst) was highest between meadow and smooth bromegrass and lowest between smooth and hybrid bromegrass. The hybrid population was genetically intermediate to smooth bromegrass and meadow bromegrass, but closer to smooth bromegrass, possibly reflecting the selection criteria used in the development of this hybrid. 相似文献
998.
Effectivness of the hybrid-weakness caused by a complementary interaction of two dominant genes Hwc1 and Hwc2 to suppress the spread of the red-grain gene into ordinary white-grained rice cultivars was investigated using the Monte Carlo computer simulation method. It was shown that the weakness genes, as far as being capable of reducing the fitness of the carrier by 80 percent or more, work quite effectively to suppress the genetic contamination of white-grained cultivars due to inflow of pollen from red-grained cultivars due to inflow of pollen from red-grain gene once the gene has been incorporated in to the population in the form of red-grained progeny produced as a result of the imperfect lethality of the initial hybrid between red-and white-grained cultivars. The weakness genes have little or no effect cultivars. The weakness genes have little or no effect either, when the contamination is initiated by an inflow of seeds or seedlings from ed-grained cultivars in these situations, removal (selection)of red grained plants Is the only countermeasure to exterminate the red-grain gene. The removal of red grained plants need not be intensive if carried out continuously. The spread of the red-grain gene after initial contamination is suppressed if the red-grain gene and weakness genes are linked. The suppressing effect of the linkage, however, is not substantial when the outcrossing rate is around or smaller than 0.01 as can be assumed in most rice cultivars. 相似文献
999.
1000.
A. Al-Sheikh J.A. Delgado K. Barbarick R. Sparks M. Dillon Y. Qian G. Cardon 《Soil & Tillage Research》2005,81(2):227-238
The potential for wind erosion in South Central Colorado is greatest in the spring, especially after harvesting of crops such as potato (Solanum tuberosum L.) that leave small amounts of crop residue in the surface after harvest. Therefore it is important to implement best management practices that reduce potential wind erosion and that we understand how cropping systems are impacting soil erosion, carbon dynamics, and properties of rangeland sandy soils. We evaluate the effects of cropping systems on soil physical and chemical properties of rangeland sandy soils. The cropping system included a small grain–potato rotation. An uncultivated rangeland site and three fields that two decades ago were converted from rangeland into cultivated center-pivot-irrigation-sprinkler fields were also sampled. Plant and soil samples were collected in the rangeland area and the three adjacent cultivated sites. The soils at these sites were classified as a Gunbarrel loamy sand (Mixed, frigid Typic Psammaquent). We found that for the rangeland site, soil where brush species were growing exhibited C sequestration and increases in soil organic matter (SOM) while the bare soil areas of the rangeland are losing significant amounts of fine particles, nutrients and soil organic carbon (SOM-C) mainly due to wind erosion. When we compared the cultivated sites to the uncultivated rangeland, we found that the SOM-C and soil organic matter nitrogen (SOM-N) increased with increases in crop residue returned into the soils. Our results showed that even with potato crops, which are high intensity cultivated cropping systems, we can maintain the SOM-C with a rotation of two small grain crops (all residue incorporated) and one potato crop, or potentially increase the average SOM-C with a rotation of four small grain crops (all residue incorporated) and one potato crop. Erosion losses of fine silt and clay particles were reduced with the inclusion of small grains. Small grains have the potential to contribute to the conservation of SOM and/or sequester SOM-C and SOM-N for these rangeland systems that have very low C content and that are also losing C from their bare soils areas (40%). Cultivation of these rangelands using rotations with at least two small grain crops can reduce erosion and maintain SOM-C and increasing the number of small grain crops grown successfully in rotation above two will potentially contribute to C and N sequestration as SOM and to the sequestration of macro- and micro-nutrients. 相似文献