MED12, the mediator complex subunit 12 gene, is mutated at high frequency in uterine leiomyomas     

Mäkinen N  Mehine M  Tolvanen J  Kaasinen E  Li Y  Lehtonen HJ  Gentile M  Yan J  Enge M  Taipale M  Aavikko M  Katainen R  Virolainen E  Böhling T  Koski TA  Launonen V  Sjöberg J  Taipale J  Vahteristo P  Aaltonen LA 《Science (New York, N.Y.)》2011,334(6053):252-255

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Congestive heart failure and atrial fibrillation in a cat with myocardial fibro-fatty infiltration     

Harjuhahto TA  Leinonen MR  Simola OT  Järvinen AK  Rajamäki MM 《Journal of Feline Medicine and Surgery》2011,13(2):109-111

Congestive heart failure and atrial fibrillation were diagnosed in a 4-year-old castrated Birman cat with progressive signs of dyspnea, tachypnea, and lethargy. Echocardiography revealed massive right-sided heart dilatation with ascites and hydrothorax. Electrocardiogram recording showed atrial fibrillation. Medical therapy with diuretics, benazepril, and antithrombotic agents was unsuccessful. The owner requested euthanasia. In post-mortem examination, changes associated with myocardial fibro-fatty infiltration were confirmed. Changes were most marked in the right ventricular wall but with left ventricular involvement was detected.  相似文献   

Peat Biofilters in Long-Term Experiments for Removing Odorous Sulphur Compounds   总被引：1，自引：0，他引：1  

Tarja Hartikainen  Pertti J. Martikainen  Minna Olkkonen  Juhani Ruuskanen 《Water, air, and soil pollution》2002,133(1-4):335-348

Peat was employed as a biological filter material for the removal of hydrogen sulphide (H_2> S) dimethyl sulphide (Me₂S) and methyl mercaptan (MeSH) from odorous ventilation air. Removal of H₂S was possible without the need to inoculate the filter material with oxidizing microbes whereas Me₂S required inoculation for degradation. The removal of Me₂S was decreased as a result of MeSH addition. Liming of the filter material, i.e., addition of calcium hydroxide, increased removal efficiency and activated the oxidation of H₂S without any adaptation period. As a consequence of the poor Me₂S removal efficiency of natural peat, a limed and inoculated biofilter was needed to purify mixtures of gases containing sulphur. The highest H₂S load tested with limed but otherwise natural peat was 136 g-S m^-3 day^-1, yielding a 99 % reduction. The maximum Me₂S elimination capacity with limed and inoculated peat was 175 g-S m^-3day^-1, but removal became unstable when the load exceeded 150 g-S m^-3day^-1. The maximum MeSH load tested was 107 g-S m^-3day^-1, yielding 98 % removal.  相似文献   

Needle Structures and Epiphytic Microflora of Scots Pine (Pinus Sylvestris L.) under Heavy Ammonia Deposition from Fur Farming     

Bäck  Jaana  Turunen  Minna  Ferm  Ari  Huttunen  Satu 《Water, air, and soil pollution》1997,100(1-2):119-132

Scots pine (Pinus sylvestris) needles from forest sites differing in distance from big fur farms emitting large amounts of ammonia and ammonium (=NH_y) were examined by scanning and transmission electron microscopy. Specific features indicating an ammonium-nitrogen overload, such as an abundance of needle surface organisms and modifications in cellular and wax structures, were classified. Throughout the study area (up to 1000 m from the farms), mesophyll cells had thin cytoplasm and folding plasmalemma indicating frost damage. Phloem damage attributable to a possible nutrient imbalance was also observed. Chloroplast membranes were undulating and the occurrence of leaf surface organisms (e.g. aerophilic algae) was more abundant at the closest sites. The changes were related both to the direct effects of dry NH_y deposition on the needles, and to the effects operating via soil acidification. The needle epicuticular waxes proved to be structurally rather inert against the influence of ammonium compounds, since no significant changes due to NH_y were observed.  相似文献   

Results of ophthalmologic screening examinations of German Pinschers in Finland – a retrospective study     

Minna Leppänen  Jerker Mårtenson†  Katariina Mäki‡ 《Veterinary ophthalmology》2001,4(3):165-169

Objective To retrospectively review ophthalmologic findings in German Pinschers in Finland. Animals studied One hundred and twenty‐two German Pinschers that had ophthalmologic examination performed according to the Finnish Kennel Club’s Eye Scheme before June 15, 1999. Procedures A total of 154 eye examination reports of 122 dogs were analysed and all described findings were reported. Results Persistent hyperplastic tunica vasculosa lentis (PHTVL) was diagnosed in 8.4% of all cases, and hereditary cataract (HC) in 6.5%. Even higher numbers of similar changes were reported by ophthalmologists; some of the dogs, however, were officially diagnosed as ‘free of symptoms’ of inherited ocular diseases. A relatively high number (4.5%) of dogs had reported changes in Y sutures. A pedigree analysis suggests recessive inheritance for both diseases. Because of missing information about many dogs in the pedigree, an autosomal incomplete inheritance pattern cannot be ruled out in either case. Conclusions Both HC and PHTVL are inherited diseases in German Pinschers. Further studies are needed to determine the importance of the changes found in Y sutures. Discrepancies between the official diagnosis and described changes are probably partially due to the lack of familiarity with the published literature concerning this breed. Further studies are needed to ascertain the inheritance pattern for both diseases. So far breeding with affected animals should be avoided.  相似文献   

Elevated levels of fragmented laminin-5 gamma2-chain in bronchoalveolar lavage fluid from dogs with pulmonary eosinophilia     

Rajamäki MM  Järvinen AK  Sorsa TA  Tervahartiala TI  Maisi PS 《Veterinary journal (London, England : 1997)》2006,171(3):562-565

Inflammation causes epithelial cell sloughing and basement membrane (BM) exposure in canine pulmonary eosinophilia (PE), leading to degradation of the epithelial cell attachment component, laminin-5 gamma2-chain, into small molecular weight fragments. The subsidence of inflammation after treatment down-regulates degradation. Laminin-5 gamma2-chain levels and molecular forms in bronchoalveolar lavage fluid (BALF) were analysed semiquantitatively by Western immunoblotting to compare PE affected (n=20) and healthy dogs (n=16) as well as PE dogs (n=6) before and after corticosteroid treatment. PE dogs expressed significantly elevated levels of total (P<0.01), 36 kDa (P<0.05) and 53 kDa (P<0.05) laminin-5 gamma2-fragments. The 36 Da fragment decreased significantly (P<0.05) after treatment. The laminin-5 gamma2-chain degradation products may be linked to epithelial cell sloughing and BM exposure or healing.  相似文献   

Does canopy mean nitrogen concentration explain variation in canopy light use efficiency across 14 contrasting forest sites?     

Peltoniemi M  Pulkkinen M  Kolari P  Duursma RA  Montagnani L  Wharton S  Lagergren F  Takagi K  Verbeeck H  Christensen T  Vesala T  Falk M  Loustau D  Mäkelä A 《Tree physiology》2012,32(2):200-218

The maximum light use efficiency (LUE?=?gross primary production (GPP)/absorbed photosynthetic photon flux density (aPPFD)) of plant canopies has been reported to vary spatially and some of this variation has previously been attributed to plant species differences. The canopy nitrogen concentration [N] can potentially explain some of this spatial variation. However, the current paradigm of the N-effect on photosynthesis is largely based on the relationship between photosynthetic capacity (A(max)) and [N], i.e., the effects of [N] on photosynthesis rates appear under high PPFD. A maximum LUE-[N] relationship, if it existed, would influence photosynthesis in the whole range of PPFD. We estimated maximum LUE for 14 eddy-covariance forest sites, examined its [N] dependency and investigated how the [N]-maximum LUE dependency could be incorporated into a GPP model. In the model, maximum LUE corresponds to LUE under optimal environmental conditions before light saturation takes place (the slope of GPP vs. PPFD under low PPFD). Maximum LUE was higher in deciduous/mixed than in coniferous sites, and correlated significantly with canopy mean [N]. Correlations between maximum LUE and canopy [N] existed regardless of daily PPFD, although we expected the correlation to disappear under low PPFD when LUE was also highest. Despite these correlations, including [N] in the model of GPP only marginally decreased the root mean squared error. Our results suggest that maximum LUE correlates linearly with canopy [N], but that a larger body of data is required before we can include this relationship into a GPP model. Gross primary production will therefore positively correlate with [N] already at low PPFD, and not only at high PPFD as is suggested by the prevailing paradigm of leaf-level A(max)-[N] relationships. This finding has consequences for modelling GPP driven by temporal changes or spatial variation in canopy [N].  相似文献   

Kidney-derived proteins in urine as biomarkers of induced acute kidney injury in sheep     

Palviainen M  Raekallio M  Rajamäki MM  Linden J  Vainio O 《Veterinary journal (London, England : 1997)》2012,193(1):287-289

Acute kidney injury (AKI) is a life-threatening condition for which an early diagnosis is problematic. The aim of the present study was to identify kidney-derived urinary proteins specific to AKI in sheep. AKI was induced in six sheep by an overdose of ketoprofen. Six untreated sheep served as controls. Urine samples were collected for up to 24 h after drug administration and pooled according to time and treatment. Tissue samples from kidney were taken immediately after euthanasia. Urinary proteins were separated by two-dimensional gel electrophoresis (2DE) and the proteins of interest were identified by mass spectrometry. Calbindin-D28k, retinol-binding protein 4 and CD1d were identified in ketoprofen-treated sheep, but not in controls. In addition, calbindin-D28k and CD1d were localized in kidney tissues by immunohistochemical staining. These preliminary results suggest that urinary calbindin-D28k and CD1d represent potential useful biomarkers of AKI, at least in sheep.  相似文献   

Genetic parameters and single versus multi-trait evaluation of udder health traits     

Enyew Negussie  Minna Koivula  Esa A. Mäntysaari 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(2):73-82

Abstract

Genetic parameters were estimated for lactation average somatic cell score (SCS) and clinical mastitis (CM) for the first three lactations of multiparous Finnish Ayrshire cows. A multi-trait linear sire model was used for estimation of covariance components, and the efficiencies of single- versus multi-trait multi-lactation (MT) sire evaluations were compared. Heritability of SCS and CM in the first three lactations ranged from 0.11 to 0.13 and 0.02 to 0.03, respectively. Within lactation, genetic correlations between SCS and CM ranged from 0.68 to 0.72. Within both traits, across-lactation genetic correlations were lowest between 1 and 3, and highest between 2 and 3, with estimates ranging from 0.75 to 0.86 and from 0.81 to 0.98 for CM and SCS, respectively. Residual and phenotypic correlations were low and ranged from 0.09 to 0.13 and from 0.10 to 0.13, respectively. The absolute difference between genetic and residual correlations was from 0.5 to 0.6. Within-lactation genetic correlations between traits that are much less than unity suggest a multi-trait model for genetic evaluation of mastitis resistance. Comparison of model prediction performance between single-trait (ST) and MT models using a data splitting method showed that the MT model was more stable in predicting breeding values in future records of animals. Especially, for young sires and CM, the SD of EBVs from the MT model was 14 to 23% higher than the ST model, indicating more effective use of information in terms of revealing more genetic variation.  相似文献   

Evaluation on Inducible Effect of pOp6 Promoter n Transgenic Rice     

Rouyi CHEN  Changxiang ZHENG  Jiang CHENG  Minna PAN  Mariena KETUDAT-CAIRNS 《农业科学与技术》2014,(5):742-744

In order to analyze the Oslbglu4 phenotype, the inducible promoter of the transgenic rice which knock-down the Oslbglu4 expression was assessed. The result showed that 30 μM dexamethasone（DEX） had the stronger induction effect than 10 μM DEX by β-Glucuronidase （GUS） staining, qRT-PCR further verified the Oslbglu4 gene deletion. The effect of DEX and its solvent absolute ethanol on seed development was measured, and no significant effect was observed. The conclusion is that final concentration of DEX at 30 μM is suitable for pOp6 promoter induction.  相似文献