Interval mapping and meta-QTL analysis of grain traits in common wheat (Triticum aestivum L.)     

S. Tyagi  R. R. Mir  H. S. Balyan  P. K. Gupta 《Euphytica》2015,201(3):367-380

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Multiorgan engraftment of human somatic cells in swine foetuses after intra-blastocyst transplantation     

Estrada J  Li P  Mir B 《Reproduction in domestic animals》2011,46(4):630-635

Adult human stem cells, mainly from hematopoietic lineage, have been injected into developing pre-immune animal foetuses, and xenogenic engraftment of liver and other organs has been reported. We isolated a rare cell population from adult human liver, fat and skin. Colonies with few cells became visible as early as 2-3 days, and a fully formed colony took 10-14 days to form. These colonies were named as liver-derived cell lines (LDCs), fat-derived cell lines (FDCs) and skin-derived cell lines (SDCs). All these cells express few pluripotency markers like Klf4, c-myc and Sox2. Pig blastocysts were injected with LDCs, FDCs and SDCs and transferred to recipient pigs. We achieved an overall pregnancy rate of 71.4% at day 35. The foetuses were analysed for human cell chimerism in liver, kidney and heart both by RT-PCR and real-time PCR using primers specific to human and pig mitochondrial DNA. The percentage of foetuses showing chimerism was 17.4% (4/23), 12.5% (2/16) and 11.1% (1/9) for LDCs, FDCs and SDCs, respectively. Of these, 42.9% (three out of seven) showed chimerism in liver and 71.4% (five out of seven) showed kidney chimerism. However, we did not detect any chimerism in the heart. The level of chimerism varied and was in the range of one human cell per one hundred thousand to one million pig cells.  相似文献   

Feeding value of rice distiller’s dried grains with solubles as protein supplement in diet of laying hens     

Gupta  Sneh Lata  Tyagi  Pramod K.  Mir  Nasir Akbar  Dev  Kapil  Begum  Jubeda  Mandal  A. B.  Tyagi  Praveen K. 《Tropical animal health and production》2020,52(3):1229-1237

A feeding trial of 10 weeks duration was undertaken on laying hens (n = 240) to evaluate feeding value of rice distiller’s dried grains with soluble (rDDGS) with or without enzyme supplementation (α-amylase, β-glucanase, xylanase, carboxymethylcellulase, pectinase, proteinase, α-galactosidase, β-galactosidase, lipase, and phytase), following 4 × 2 factorial design, on egg production, nutrient utilization, and cost economics of egg production. The birds were randomly assigned to eight dietary treatments with 30 birds/treatment. The birds were housed individually in layer cages and each bird was taken as an experimental unit. Eight experimental diets were prepared by incorporating four levels (0, 50, 75, and 100 g/kg) of rDDGS with and without enzyme supplementation. The results revealed a significant (P < 0.01) increase of egg mass, feed intake, egg production, and body weight gain in dietary treatments with up to 75 g rDDGS though the values were statistically similar to the hens fed 100 g rDDGS. Enzyme supplementation resulted in significant (P < 0.01) improvement of egg mass, egg production, feed conversion ratio (FCR) per dozen eggs, FCR per kilogramme egg mass, and net FCR. The significantly (P < 0.01) higher yolk index was observed at 100 g rDDGS level, while shell thickness improved significantly (P < 0.01) up to 75 g rDDGS level. No significant effect of rDDGS inclusion was observed on shape index, albumin index, and Haugh unit. Enzyme supplementation significantly improved the shell thickness and yolk colour of eggs. Nitrogen, calcium, and phosphorus retention and dry matter metabolizability did not show any significant treatment effects. There was significant (P < 0.01) reduction in feed-cost per kilogramme egg mass or per dozen eggs with the increased DDGS levels and dietary enzyme supplementation. It was concluded that rDDGS can be used up to 100 g/kg diet of laying hens along with enzyme supplementation for better productivity of layer hens.

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Examination of jackass (Equus asinus) accessory sex glands by B-mode ultrasound and of testicular artery blood flow by colour pulsed-wave Doppler ultrasound: Correlations with semen production     

Sabrina Gacem  Marion Papas  Jaime Catalan  Jordi Miró 《Reproduction in domestic animals》2020,55(2):181-188

The accessory sex glands play a major role in the production of seminal plasma, and testicular artery blood flow seems to strongly influence testicular function. However, very little ultrasound imaging of these organs has been undertaken in donkeys. The present work reports the results of such examinations in five jackasses along the year. The accessory glands were inspected by B-mode ultrasound while the testicular artery blood flow was assessed by colour pulsed-wave Doppler ultrasound. The testicular artery was examined at pampiniform plexus (PPT), supratesticular area (ST) and capsular artery (CA). Values were recorded for the total arterial blood flow (TABF), peak systolic velocity (PSV), end-diastolic velocity (EDV), resistive index (RI), pulsatility index (PI) and time average maximum velocity (TAMV). Semen was obtained and assessed for sperm concentration, viability, abnormalities and motility using a CASA system. The bulbourethral glands, prostate and ductus deferens ampullae were relatively larger than in the stallion. Bulbourethral glands and ampullae sizes were inversely correlated with sperm motility. An reduction in blood flow between the level the PPP and the CA was observed, helping to reduce testis temperature and oxygen pressure. Blood flow at the CA showed the strongest correlation with semen production. PI and RI were positively correlated with the CASA motility variable STR (p = .02, p = .06) and sperm viability (p = .01), while sperm concentration (p = .05) correlated inversely with PSV, EDV, TAMV and TABF. EDV also correlated negatively with the CASA variables VSL, LIN, STR and VAP (p ≤ .05). PI and RI were also negatively correlated with testis length (p = .0093, p = −.0438).  相似文献   

Genetic parameters of biokinematic variables of the trot in Spanish Purebred horses under experimental treadmill conditions     

Valera M  Galisteo AM  Molina A  Miró F  Gómez MD  Cano MR  Agüera E 《Veterinary journal (London, England : 1997)》2008,178(2):219-226

The purpose of this study was to estimate the genetic parameters of biokinematic variables in Spanish Purebred (SPB) horses in order to select those of sufficient interest to be measured in the pre-selection of the animals for possible inclusion in the breeding programme. Kinematic analysis of 130 SPB horses 4.6+/-1.5 years old were recorded at the trot (4m/s) on a treadmill. Genetic parameters were estimated using VCE software and a bivariate mixed animal model including age and stud as fixed effects and animal additive genetic effect and residual error as random effects. In general, heritabilities were high (0.33-0.88). The angular variables presented the lowest heritabilities, whereas the maximum height of the fore-hoof and the duration of swing phase in the hindlimb gave the highest scores. Genetic correlations were also very high, so it was possible to reduce the number of breeding programme characteristics to stride duration, hindlimb swing phase duration, range of stifle and elbow angles, minimal angle of carpus, and minimal retraction-protraction angle of the hindlimb.  相似文献   

Genetic relationships among American donkey populations: insights into the process of colonization          下载免费PDF全文

J. Miró  F. Goyache  A. Loarca  O.R. Martínez López  J.L. Canelón  A. Stemmer  L. Aguirre  M.A.C. Lara  L.A. Álvarez  S. Llambí  N. Gómez  L.T. Gama  M.F. Nóvoa  R.D. Martínez  E. Pérez  A. Sierra  M.A. Contreras  A.M. Guastella  D. Marletta  G. Arsenos  I. Curik  V. Landi  A. Martínez  J.V. Delgado 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2016,133(2):155-164

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Prevalence and seasonal variation of ectoparasite load in free-range chicken of Kashmir valley     

S. T. Salam  M. S. Mir  A. R. Khan 《Tropical animal health and production》2009,41(7):1371-1376

A faunistical study of ectoparasites was carried out for a period of two years from January 2005 to December 2006 in rural free-range chicken purchased from different areas of Kashmir valley. Screening of a total of 478 birds revealed only lice infestation with an overall prevalence of about 100% and 97.69% birds harboring multiple species. The prevalence for various species of lice, during winter, spring, summer, autumn and overall prevalence respectively, was 90.32%, 99.14%, 100%, 98.34% and 96.86% for Lipeurus caponis; 33.87%, 48.71%, 57.75%, 39.66% and 44.76% for Goniodes gigas; 29.83%, 32.47%, 45.68%, 32.23% and 34.93% for Menopon gallinae; 28.22%, 32.47%, 39.65%, 32.23% and 33.05% for Menacanthus cornutus; 16.12%, 19.65%, 25%, 18.18% and 19.66% for Goniocotes gallinae and 6.45%, 12.82%, 13.79%, 4.95% and 9.41% for Eomenacanthus stramineus. The seasonal influence on nature and intensity of infestation was evident.  相似文献   

Detection of zoonotic intestinal parasites in public parks of Spain. Potential epidemiological role of microsporidia     

Dado D  Izquierdo F  Vera O  Montoya A  Mateo M  Fenoy S  Galván AL  García S  García A  Aránguez E  López L  del Águila C  Miró G 《Zoonoses and public health》2012,59(1):23-28

Several studies have demonstrated that the soil of public parks presents an important source of infection which has a significant impact on public health. Children are the main group affected by accidentally ingestion of contaminated soil. This study was performed in order to identify the presence of zoonotic parasites in dog and cat faecal and soil samples from public parks of Madrid, Spain. Six hundred twenty-five and seventy-nine soil and faecal samples (presumably from dogs and cats) respectively were collected from 67 parks. Intestinal parasites were identified in 27 parks (40.3%), which were contamined with Giardia sp. (19.4%), microsporidia (19.4%), Toxocara spp. (16.4%), Cryptosporidium sp. (6%), Entamoeba histolytica (3%) and Ancylostomidae (3%). Combinations of two or more intestinal parasites were found in 11 parks, and it was common to find Giardia and microsporidia together in samples. Intestinal parasites were detected in 18% (112/625) of soil samples. The most frequent parasite species found in the examined soil samples were Toxocara spp. (16.4%), followed by Giardia sp. (4.5%) and Strongyloides sp. larvae (3%). The zoonotic parasites found in the 79 faecal samples were Giardia sp. (17.7%), Cryptosporidium sp. (9%), E. histolytica (2.5%), Trichuris vulpis (1.3%), Toxascaris leonina (1.3%) and microsporidia spores (28%). Microsporidia characterization by amplification of DNA confirmed 10 samples as positive, eight for E. bieneusi and two for E. hellem by PCR. The role of those parasites in the environment are discussed.  相似文献   

Feeding flaxseed in grass hay and barley silage diets to beef cows increases alpha-linolenic acid and its biohydrogenation intermediates in subcutaneous fat     

He ML  McAllister TA  Kastelic JP  Mir PS  Aalhus JL  Dugan ME  Aldai N  McKinnon JJ 《Journal of animal science》2012,90(2):592-604

The objective of the study was to determine temporal fat deposition and fatty acid profiles in beef cows fed hay- or barley silage-based diets, with or without flaxseed. Crossbred cull beef cows (n = 64, >30 mo of age, 620 ± 5 kg) were removed from grassland pastures, randomly assigned to 16 pens, and given ad libitum access to 50:50 (wt/wt, DM basis) forage:concentrate diets containing 0 or 15% ground flaxseed (DM basis, 5.2% added fat). Diets consisted of hay control (HC), hay+flaxseed (HF), barley silage control (SC), and silage+flaxseed (SF). Backfat biopsies were obtained from each cow at 0, 6, and 12 wk, and at slaughter (~20 wk) to assess fatty acid composition. With the exception of feed efficiency, flaxseed × forage interactions were not significant for backfat accumulation or performance parameters. Flaxseed improved (P < 0.01) feed conversion when supplemented to hay-based diet and increased ADG (P = 0.03), resulting in a heavier (P = 0.02) BW. Compared with hay, barley silage increased (P < 0.01) DMI, ADG, and feed efficiency. Subcutaneous fat contained 0.68% n-3 fatty acids at wk 0, and reached 0.68, 0.81, and 0.94% in HF cows after 6, 12, and 20 wk, respectively (Y(n-3) = 0.0133X + 0.6491, r = 0.87). It was 0.67% at wk 0, and reached 0.65, 0.77, and 0.90% in SF cows after 6, 12, and 20 wk, respectively (Y(n-3) = 0.0121X + 0.6349, r = 0.75). In contrast, weight percentage of n-3 fatty acids decreased in HC cows from 0.63, 0.50, and 0.47, to 0.43%, and in SC cows from 0.63, 0.40, and 0.36, to 0.33% over the 20 wk. A forage × flaxseed interaction (P < 0.05) occurred for many of the α-linolenic acid (ALA) biohydrogenation intermediates, including vaccenic acid (C18:1 trans-11) and CLA (combined C18:2 trans-7,cis-9 and cis-9,trans-11) in plasma, and in subcutaneous fat this also included non-CLA dienes. Concentrations of most α-linolenic acid biohydrogenation intermediates were greater when feeding flaxseed with hay. In conclusion, forage source altered plasma concentrations and rate of accumulation of ALA biohydrogenation products in subcutaneous fat from beef cows fed flaxseed. Factors responsible for this response are yet to be defined, but may include forage-mediated changes in ruminal biohydrogenation of ALA, as well as alterations in fatty acid metabolism and deposition.  相似文献   

ESBL- and plasmidic class C beta-lactamase-producing E. coli strains isolated from poultry, pig and rabbit farms     

Blanc V  Mesa R  Saco M  Lavilla S  Prats G  Miró E  Navarro F  Cortés P  Llagostera M 《Veterinary microbiology》2006,118(3-4):299-304

This study aims to determine the presence of extended-spectrum (ESBL) and plasmidic class C beta-lactamase-producing Enterobacteriaceae in poultry, pig and rabbit farms of Catalonia (Spain). PFGE typing showed a low clonal relationship among strains carrying these mechanisms of resistance. Ninety-three percent of them were resistant to two or more of the non-beta-lactam antimicrobials tested and harboured ESBL and plasmidic class C beta-lactamases. Greater diversity of these enzymes was found in strains from poultry farms, the CTX-M-9 family, especially CTX-M-14, with CMY-2 being the most frequent. The isolation of TEM-52 and SHV-2-producing Escherichia coli strains from these animal farms is noteworthy. In contrast, 73% of the strains from pig farms had CTX-M-1, and neither the CMY-type nor CTX-M-9 family enzyme was found. Likewise, it is the first time that CTX-M-1 and SHV-5 encoding strains have been isolated in pigs. On the other hand, in rabbit farms CTX-M-9 family was also the most frequent, being detected in three of a total of four strains. The last one showed a CMY-2, for the first time detected in these animals, too. In conclusion, commensal E. coli strains of food-producing animal farms are a reservoir of ESBL and plasmidic class C beta-lactamases.  相似文献