Genomic analysis of Theileria sergenti stocks in Japan with DNA probes.     

T Matsuba  Y Kawakami  H Iwai  M Onuma 《Veterinary parasitology》1992,41(1-2):35-43

Restriction fragment length polymorphisms of Theileria sergenti DNA from 18 different infections of cattle in 14 locations in Japan were analyzed by Southern blotting using T. sergenti genomic DNA fragments as probes. Probe pTs 2 hybridized with four fragments in BamHI digested piroplasm DNA, at 8.0, 7.3, 6.0 and 3.4 kb. Probe pTs 11-D1 hybridized with multiple fragments. With each probe, polymorphisms were observed among stocks from different locations. However, there was no correlation between the patterns of hybridization bands and the locations where parasites were collected. Analysis of the hybridization patterns of stocks obtained from individual cattle in the same grazing areas showed an almost identical pattern.  相似文献   

Application of complement-dependent antibody cytotoxicity test for diagnosis of enzootic bovine lymphosarcoma     

M Suzuki  K Okada  K Ohshima  S Numakunai  Y Aida  M Onuma 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(5):841-846

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Evaluation of fetal growth and estimation of fetal age based on skeletal growth in Hokkaido sika deer (Cervus nippon yesoensis Heude, 1884)     

Kobayashi A  Onuma M  Yokoyama M  Suzuki M  Uno H  Ohtaishi N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1535-1542

We investigated fetal development and the estimation of fetal age of 127 Hokkaido sika deer fetuses, categorizing them into three groups according to the nutritional condition of populations. The order and time of the appearance of ossification centers were clarified, and fetal age was determined based on bone length and the appearance of ossification centers. Then we observed the differences in fetal growth among three populations, and discussed the effect of poor nutrition on the fetal growth. The results suggest that fetal diaphysial length of the femur was affected very little by nutritional conditions, whereas conception dates were delayed and fetal weight was restricted as the nutritional condition became poorer. Although it is impossible to know the exact accurate fetal age in wild populations, it was possible to create a standard to estimate fetal age more precisely by the method described in this study. Both the bone length and the appearance of ossification centers are reliable indices to estimate fetal age precisely in measurements available from fetuses of unknown age, and can be applied to estimate the fetal age of other populations of sika deer, whereas estimation of fetal age based on weight is prone to great errors.  相似文献   

Complete cDNA sequences and phylogenetic analyses of the Th1 and Th2 cytokines of the bactrian camel (Camelus bactrianus)     

Odbileg R  Purevtseren B  Batsukh Z  Konnai S  Ohashi K  Onuma M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(9):941-946

The complementary DNAs of the Th1 (IL-2, IL-12p35, and IFN-gamma) and Th2 (IL-4, IL-10 and IL-13) cytokine genes of the bactrian camel (Camelus bactrianus) were cloned, sequenced, and analyzed. IL-2, IL-4, IL-10, IL-12p35, IL-13, and IFN-gamma were found to have 465, 402, 537, 669, 411, and 501 bp length open reading frames with 154, 133, 178, 222, 136, and 166 amino acid encodings, respectively. The homology ranged from 58.8% to 100% between the nucleotide sequences of the camel cytokine genes and the published sequences of other mammalian genes, including the llama, pig, cow, horse, human, and mouse. The cDNA had highest homology with orders Artiodactyla (pigs and cattle) and Perissodactyla (horses), especially to the recently cloned llama sequences.  相似文献   

The presence of a short form of p53 in chicken lymphoblastoid cell lines during apoptosis     

Takagi M  Takeda T  Asada Y  Sugimoto C  Onuma M  Ohashi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(6):561-566

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Tail root osteosarcoma in a chipmunk (Tamias sibiricus)     

Tamaizumi H  Kondo H  Shibuya H  Onuma M  Sato T 《Veterinary pathology》2007,44(3):392-394

An 8-year-old chipmunk (Tamias sibiricus) showed a mass on the tail root. Histologically, the excised mass showed proliferation of spindle-to-polyhedral cells and abundant multinucleated giant cells, with the production of neoplastic osteoid. Immunohistochemically, neoplastic cells were positive for vimentin and weakly positive for osteocalcin. Osteoid was also positive for osteocalcin. This tumor was diagnosed as a giant cell-type osteosarcoma. This is the first case report of osteosarcoma in a chipmunk.  相似文献   

Spontaneous osteosarcoma in a rabbit ( Oryctolagus cuniculus)     

Kondo H  Ishikawa M  Maeda H  Onuma M  Masuda M  Shibuya H  Koie H  Sato T 《Veterinary pathology》2007,44(5):691-694

A 6-year-old male cross-breed rabbit ( Oryctolagus cuniculus) was presented with lameness and severe swelling from the right shoulder to brachium. On 16-detector helical computed tomography images of the amputated right forelimb after being fixed in formalin, evident proliferative and destructive lesions of bone were observed. On histologic examination, the tumor was composed of proliferating neoplastic cells that resembled histiocytes, with abundant osteoid production. A large number of multinucleated giant cells were found throughout. This case was diagnosed as osteosarcoma by clinical, radiographic, and histologic findings. This is a rare case report of osteosarcoma in a rabbit consistent with canine predilection sites.  相似文献   

Antibodies produced by mice immunized with recombinant vaccinia viruses expressing two different types of a major Theileria sergenti surface antigen (p32) react with the native surface antigen.     

Y Takasima  X Xuan  Y Matsumoto  M Onuma  H Otsuka 《Veterinary parasitology》1999,84(1-2):65-73

A 32 kDa major surface antigen, p32, of Theileria sergenti at the piroplasm stage is the main target of the host immune response. The immunogenic property of the p32 varies in some strains among the population of Theileria sergenti in Japan where the Chitose type and the Ikeda type are the most common varieties. We have constructed vaccinia virus recombinants vv/p32C and vv/p32I which harbor the Chitose and Ikeda types of p32 gene, respectively. It was found that vv/p32C and vv/p32I produced type-specific p32 which did not cross react with the monoclonal antibodies (mAbs) against the other type of p32. When mice were immunized with vv/p32C and vv/p32I, antibodies against p32 were detectable 2 weeks after the immunization, and these antibodies reacted with the native surface antigen in purified T. sergenti merozoite.  相似文献   

Reproductive cycle observation of the Okinawa rail (Gallirallus okinawae) in the wild     

Onuma M  Nagamine T  Nakaya Y  Neagari Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1169-1175

The captive breeding program of the Okinawa rail started in 2008. For successful captive breeding, information related to reproduction, such as age at sexual maturity, testicular cycles and ovulatory cycles, is essential to predict when reproduction is possible and when certain reproductive behaviors are most likely to occur. We made gross and histological observations of the reproductive organs of Okinawa rails to gain understanding of sexual maturity, the testicular cycle and the ovulatory cycle. We found that the weight of the testis was smallest in December and largest in March. Changes in the diameter of the seminiferous tubule showed the same pattern. Mature sperm were observed from March to June. The heaviest ovary was observed in April. A single peak of reproduction, from March to April, was observed in males and females. Our observations suggested that the Okinawa rail is a seasonal breeder. Establishing suitable breeding pairs will be critical to ensure success of the Okinawa rail captive breeding program. Our results suggested that pairing must be started before March. If supportive breeding is used, semen should be collected from March to June and artificial insemination conducted in April.  相似文献   

Molecular detection of Anaplasma phagocytophilum in cattle and Ixodes persulcatus ticks     

Murase Y  Konnai S  Hidano A  Githaka NW  Ito T  Takano A  Kawabata H  Ato M  Tajima T  Tajima M  Onuma M  Murata S  Ohashi K 《Veterinary microbiology》2011,149(3-4):504-507

The tick-borne pathogen, Anaplasma phagocytophilum (A. phagocytophilum), the causative agent of human granulocytic anaplasmosis (HGA), is increasingly becoming a public health concern as an aetiological agent for emerging infectious disease. We found A. phagocytophilum infection in a pooled sample of field-collected Ixodes persulcatus (I. persulcatus) ticks from one district in Hokkaido, Japan. Thus, to further investigate the prevalence in field-collected ticks, we used PCR assays targeting the A. phagocytophilum gene encoding 44 kDa major outer membrane protein (p44) for screening of I. persulcatus ticks and samples from cattle from pastures. Out of the 281 I. persulcatus ticks, 20 (7.1%) were found to harbor A. phagocytophilum DNA. The infection rate for A. phagocytophilum in cattle was 3.4% (42/1251). In future studies, it will be necessary to investigate effects of the infection in order to understand its pathogenesis of A. phagocytophilum in domestic animals.  相似文献