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41.
Akimoto T Terada M Shimizu A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):65-70
We established the WBN/Kob-Lepr(fa) rat as a new congenic strain for the fa allele of the leptin receptor gene (Lepr). Homozygous (fa/fa) WBN/Kob-Lepr(fa) rats provide a model of non-insulin-dependent diabetes, although its onset is secondary to pancreatitis. In the present study, we compared histopathological observations of pancreatitis in each genotype of this rat, to examine its suitability as a model of pancreatitis. The histopathological findings of the pancreatitis revealed intense changes dependent on age, such as hemorrhage or hemosiderin deposition. The pancreatitis in homozygous (fa/fa) WBN/Kob-Lepr(fa) rats were more severe than those of WBN/Kob rats. 相似文献
42.
Meas S Yilmaz Z Usui T Torun S Yesilbag K Ohashi K Onuma M 《The Japanese journal of veterinary research》2003,51(1):3-8
A seroepidemiological study of bovine immunodeficiency virus (BIV) and bovine leukemia virus (BLV) infections was conducted in four different cattle herds in Turkey. A total of 300 blood samples were analyzed and 12.3% were found to be positive for anti-BIV p26 antibodies by Western blot analysis and 1.6% positive for anti-BLV gp51 antibodies by an immunodiffusion test. BIV infection was confirmed with the detection of BIV-provirus DNA using the nested polymerase chain reaction. This is the first evidence for the presence of BIV in cattle in Turkey. 相似文献
43.
Seroprevalence of bovine immunodeficiency virus and bovine leukemia virus in dairy and beef cattle in hokkaido 总被引:2,自引:0,他引:2
Usui T Meas S Konnai S Ohashi K Onuma M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(2):287-289
Serological survey of bovine immunodeficiency virus (BIV) and bovine leukemia virus (BLV) infection was conducted in dairy cattle from 10 different regions of Hokkaido, Japan. Among 390 cattle, 11.0% of cattle were BIV-seropositive and 3.3% were BLV-seropositive. Moreover, in two dairy farms, where bovine leukosis has been reported, prevalence of BIV infections were 6.4 and 9.1%, respectively. In contrast, among 150 beef cattle, 16.6% were BIV-seropositive while none was BLV-seropositive. Dual infections with BLV and BIV in dairy cattle were tested by using 107 BLV-seropositive sera, and 20 sera were found BIV-positive (18.7%). These results indicate that BIV infection was widespread in Hokkaido. 相似文献
44.
Expression of mRNA for sodium-glucose transporter 1 and fatty acid translocase in the ruminant gastrointestinal tract before and after weaning 总被引:1,自引:0,他引:1
Hideaki HAYASHI Tomo YONEZAWA Takeshi KANETANI Fuminori TERADA Kazuo KATOH Yoshiaki OBARA 《Animal Science Journal》2005,76(4):339-344
The mRNA expression of sodium‐glucose transporter 1 (SGLT1) and fatty acid translocase (CD36) in the gastrointestinal tract of Holstein cattle and Saanen goats before and after weaning was investigated. Before weaning, the expression of both SGLT1 and CD36 was highest in the jejunum, relative to the other parts of the gastrointestinal tract in both species. The expression of SGLT1 and CD36 in the duodenum was second highest in the goats. After weaning, SGLT1 and CD36 expression in the small intestine significantly decreased in both species. The expression of both types of transporters was also detected in the forestomach. From these results, it was concluded that the jejunum is probably the major absorption site for glucose and long‐chain fatty acids before weaning, and that the expression of both types of transporters decreases after weaning in cattle and goats. 相似文献
45.
Masuda Yoshitsugu Shima Yasuhiro Tamaru Osamu Takahashi Yuki Ohmura Yoshihiro Iwasaki Takashi Kamoshida Masaaki Arimoto Misao Yamano Keisuke Yatabe Takashi 《Fisheries Science》2019,85(6):1045-1054
Fisheries Science - Morphological deformities of the jaw and vertebrae arise as hindrances to seed production of Japanese eel Anguilla japonica. To obtain basic information related to development... 相似文献
46.
Nakajima C Imamura S Konnai S Yamada S Nishikado H Ohashi K Onuma M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):447-452
A novel thrombin inhibitory protein coding gene was identified from a cDNA library derived from salivary gland of partially-fed Haemaphysalis longicornis (hard tick). The gene encoded a 93-amino acid protein, designated chimadanin, which had a signal peptide sequence and was predicted to be a secretory protein. It showed no similarity to any other previously identified proteins or conserved domain sequences. The gene was expressed during blood feeding and suggested to be expressed mainly in the salivary gland. The predicted mature region of chimadanin was expressed in Escherichia coli and characteristics of the recombinant chimadanin were determined. The activated partial thromboplastin time and the prothrombin time in sheep plasma were significantly prolonged by chimadanin in a dose dependent manner. Amidolytic activity of thrombin was also inhibited by chimadanin in a dose dependent manner and it suggested that chimadanin was an anticoagulant with thrombin inhibitory activity. This newly identified thrombin inhibitor may play an important role in tick blood feeding. 相似文献
47.
Wen Zhong Zhuang Chihiro Sugimoto Shuichi Kubota Sadao Onoe Misao Onuma 《Veterinary parasitology》1995,60(3-4):191-198
Theileria sergenti piroplasms were purified from different parasitemia peaks of cattle infected with parasitized erythrocytes or sporozoites during persistent infection. Their activities with monoclonal antibodies 13F5 and C9, which recognize 23 kDa and 32 kDa piroplasm surface proteins, respectively, were analyzed. Antigenic differences were observed among parasites from different parasitemia peaks during persistent infection when cattle were infected with sporozoites. Results of two-dimensional polyacrylamide gel electrophoresis showed that the 23 and 32 kDa proteins were expressed in all samples tested, regardless of their reactivities with the monoclonal antobodies. In contrast, parasites obtained from cattle inoculated with parasitized erythrocytes showed no antigenic alteration over a 2 month observation period. The results suggest that antigenic alteration of T. sergenti during persistent infection is related to whether the parasites proliferate through extraerythorocytic schizont stage in cattle or sporozoite and other sexual stages in tick vector. 相似文献
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49.
Zhou J Ohashi K Yamasaki M Onuma M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(4):519-521
For studying protein trafficking in Babesia-infected erythrocyte, we describe the cloning of a Rab5, one of molecular marker for vesicle trafficking in eukaryotic cells, gene homologue in Babesia gibsoni (BgRab5). The full-length cDNA of BgRab5 is 1,020 bp long with an open reading frame encoding a protein of 220 amino acids. The deduced amino acid sequence of BgRab5 contained the highly conserved GTP-binding consensus sequence and shares about 40% homology with that of Rab5 from Plasmodium falciparum, Toxoplasma gondii, Dog, Lotus japonicusor, Oryza sativa. Northern blot analysis showed that the BgRab5 probe hybridized with a 1kb band in total RNA from parasitized erythrocytes, that was consistent with the size of the BgRab5 full-length cDNA. 相似文献
50.
Tsumagari S Ichikawa Y Toriumi H Ishihama K Morita M Kanamaki M Takeishi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):1003-1005
Parentage testing was performed in sixteen litters by canine artificial inseminations with frozen semen from different sires on Days 5 and 7 after the LH surge. It became apparent that only 25% of dams had superfecundation, but 43.8% of dams were whelped after insemination only on Day 5 after the LH surge and 31.3% of dams after insemination only on Day 7. Of the total 87 puppies, 46% were born after insemination on Day 5 after the LH surge and 54% after insemination on Day 7. This result strongly suggested that canine artificial insemination with frozen semen could be sufficiently successful also on Days 5 and 7 after the LH surge. 相似文献