Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses     

Lowiese MB Desmarets  Sebastiaan Theuns  Dominique AJ Olyslaegers  Annelike Dedeurwaerder  Ben L Vermeulen  Inge DM Roukaerts  Hans J Nauwynck 《Veterinary research》2013,44(1):71

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Computed tomographic anatomy of the nasal cavity,paranasal sinuses and tympanic cavity of the koala     

S Hemsley  H Palmer  RB Canfield  MEB Stewart  MB Krockenberger  R Malik 《Australian veterinary journal》2013,91(9):353-365

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Pesticidal seed coats based on azadirachtin‐A: release kinetics,storage life and performance     

Keyath Nisar  Jitendra Kumar  MB Arun Kumar  Suresh Walia  Najam A Shakil  Rajender Parsad  Balraj S Parmar 《Pest management science》2009,65(2):175-182

BACKGROUND: Infestation of seeds by pests during storage leads to deterioration in quality. Seed coating is an effective option to overcome the menace. Unlike synthetic fungicidal seed coats, little is known of those based on botanicals. This study aims at developing azadirachtin‐A‐based pesticidal seed coats to maintain seed quality during storage. RESULTS: Polymer‐ and clay‐based coats containing azadirachtin‐A were prepared and evaluated for quality maintenance of soybean seed during storage. Gum acacia, gum tragacanth, rosin, ethyl cellulose, hydroxyethyl cellulose, polyethyl methacrylate, methyl cellulose, polyethylene glycol, polyvinyl chloride, polyvinyl acetate, polyvinyl pyrrolidone and Agrimer VA 6 polymers and the clay bentonite were used as carriers. The time for 50% release (t_1/2) of azadirachtin‐A into water from the seeds coated with the different coats ranged from 8.02 to 21.36 h. The half‐life (T_1/2) of azadirachtin‐A in the coats on seed ranged from 4.37 to 11.22 months, as compared with 3.45 months in azadirachtin‐A WP, showing an increase by a factor of nearly 1.3–3.3 over the latter. The coats apparently acted as a barrier to moisture to reduce azadirachtin‐A degradation and prevented proliferation of storage fungi. Polyethyl methacrylate, polyvinyl acetate and polyvinyl pyrrolidone were significantly superior to the other polymers. Azadirachtin‐A showed a significant positive correlation with seed germination and vigour, and negative correlation with moisture content. CONCLUSION: Effective polymeric carriers for seed coats based on azadirachtin‐A are reported. These checked seed deterioration during storage by acting as a barrier to moisture and reduced the degradation of azadirachtin‐A. Copyright © 2008 Society of Chemical Industry  相似文献   

Serum testosterone,sperm quality,cytological, physicochemical and biochemical characteristics of the prostatic fraction of dogs with prostatomegaly          下载免费PDF全文

A Aquino‐Cortez  BQ Pinheiro  HVR Silva  DBC Lima  TFP Silva  MB Souza  DA Viana  FAF Xavier Júnior  JSAM Evangelista  FZ Brandão  LDM Silva 《Reproduction in domestic animals》2017,52(6):998-1003

Prostatomegaly is a common finding in older non‐neutered dogs. This study compared the serum testosterone, sperm quality and characteristics of the prostatic fraction between healthy dogs and dogs with prostatomegaly. Blood samples of ten dogs (five dogs from each group) were taken for serum testosterone measurement. Sperm motility, vigour, concentration, viability, membrane functionality and morphology were analysed in sperm‐rich fraction. Osmolality, pH, cell types, and albumin, haemoglobin, acid phosphatase, alkaline phosphatase, glucose, triglycerides, cholesterol, calcium, phosphorus, magnesium and chloride were analysed in prostatic fraction. Dogs with prostatomegaly have the lowest sperm motility, vigour, concentration and functional membrane. Dogs with prostatomegaly have the highest glucose, triglycerides and cholesterol. Glucose was the only constituent positively correlated with serum testosterone and prostate volume. It can be concluded that dogs with prostatomegaly have poorer sperm quality, and glucose, triglycerides and cholesterol in prostatic fraction can be used as prostatomegaly biomarkers.  相似文献   

Effect of Ischemia and Reperfusion on Neutrophil Accumulation in Equine Microvascular Tissue Flaps   总被引：1，自引：0，他引：1  

W. Michael Scott  DVM  MVSc  Diplomate ACVS    J. David Fowler  DVM  MVSc  Diplomate ACVS    Gilbert Matte  PhD    rew L. Allen  DVM  MVSc  PhD    A. A. Wilkinson  MA  MB  BChir  MSc  FRCPC    Jeremy V. Bailey  BVSc  MVSc  Diplomate ACVS  Peter B. Fretz  VMD  Diplomate ACVS 《Veterinary surgery : VS》1999,28(3):180-187

Objective— To investigate neutrophil accumulation after ischemia and reperfusion (IR) in microvascular tissue flaps in horses.
Study Design— Randomized controlled experiment.
Sample Population— A total of 8 horses between 1 and 10 years of age, 4 of each sex.
Methods— Control and experimental myocutaneous island flaps based on the superficial branch of the deep circumflex iliac vessels were dissected on each horse. Atraumatic vascular clamps were applied to the pedicle of the experimental flap for 90 minutes and then removed to allow reperfusion. Based on the assumption that rapid infiltration of neutrophils into affected tissues is a hallmark of IR injury, radiolabeled autogenous leukocytes were used to indirectly quantify neutrophil accumulation in flap tissues. Labeled leukocytes were administered through a jugular catheter 30 minutes before flap reperfusion. Biopsies were collected from each flap over a 6 hour postischemia time period; in group 1 (  n = 4  ) from 0 to 6 hours postischemia, and in group 2 (  n = 4  ) from 24 to 30 hours postischemia. Biopsies were examined scintigraphically and histologically for evidence of neutrophil infiltration.
Results— All control flaps survived and 6 of 8 experimental flaps survived. There was no significant evidence of acute neutrophil infiltration into flap tissues after reperfusion in either group.
Conclusions— The results of this study suggest that equine myocutaneous flap tissues can survive a 90-minute ischemic period and reperfusion. No significant evidence of the occurrence of IR injury in flap tissues was found.
Clinical Relevance— The reasons for the previously reported failures of equine free tissue transfer remain uncertain, but they do not appear to be caused by neutrophil mediated injury associated with ischemia and reperfusion.  相似文献   

Half-Body Radiotherapy     

Elizabeth J. Laing DVM  DVSc    Peter J. Fitzpatrick MB  BS  FRCP  FRCR    AIan M. Norris DVM    AIlen Mosseri MSc    Walter D. Rider MB  FRCP  FRCP    AIlen G. Binnington DVM  MSc    Angela Baur BVSc  V. E. Valli DVM  PhD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1989,3(2):96-101

Eight healthy mongrel dogs were treated with half-body irradiation (HBI) in a pilot study to evaluate the technique and radiotolerance of different organs. Cranial and caudal half-body fields were established using the 13th thoracic vertebra as the dividing point. Under general anesthesia, either 7 or 8 Gray (Gy) were delivered to one half of the body using opposing radiation portals. The other half of the body was similarly treated 28 days later. The dogs were monitored for 12 months. Significant radiation effects included transient bone marrow suppression and radiation sickness. There were no serious or life-threatening problems, but the 8 Gy group consistently showed more severe clinical signs and histologic changes than the 7 Gy group. Total body irradiation in two fractions of 7 or 8 Gy given 1 month apart appears to be a safe treatment that can be developed for therapy in veterinary oncology.  相似文献   

Microneurovascular Free Digital Pad Transfer in the Dog   总被引：1，自引：0，他引：1  

ANTHONY W. P. BASHER BVetMed  MVSc  MRCVS  DiplomateACVS    J. DAVID FOWLER DVM  MVSc  DiplomateACVS    C. VAUGHAN BOWEN MB  ChB  FRCS    EDWARD G. CLARK DVM  MVSC  DiplomateACVP  NANCY L. CROSBY BSC  AHT 《Veterinary surgery : VS》1990,19(3):226-231

By cadaver dissections, the fifth digit of the canine hind limb was determined to have a consistent neurovascular anatomy, and therefore be a suitable donor for an axial pattern digital pad flap. The defined digital pad flap was transferred to the region of an excised metacarpal pad by microneurovascular anastomoses in five operations on four dogs. One flap failure was considered to result from failure of the venous anastomosis. In all four successful transfers, cutaneous sensation was reestablished on average in 78 days. There was histologic evidence of nerve regeneration across the anastomosis in one dog at week 24. The transferred pads of three dogs monitored for 15 months underwent hypertrophic changes. There were no complications in two active dogs. In one dog, superficial ulceration of a region of the flap adjacent to the pad required surgical revision. This dog continued to show mild lameness after daily runs of 3 to 4 km.  相似文献   

Sutured End-to-End and Stapled Side-to-Side Jejunal Anastomoses in the Horse     

GARY M. BAXTER VMD  MS  Dipiomate ACVS    ROBERT J. HUNT DVM  MS    DAVID E. TYLER DVM  PhD    REW H. PARKS MA  vet MB  MRCVS  Dipiomate ACVS  BRADLEY R. JACKMAN DVM 《Veterinary surgery : VS》1992,21(1):47-55

Hand sutured end-to-end (EE) and stapled side-to-side (SS) small intestinal anastomoses were performed in 10 healthy adult horses. In five SS anastomoses, staple lines on the blind ends of the jejunum were inverted (SSI) and in five they were not (SSNI). Five EE anastomoses were sutured with polydioxanone and five were sutured with polyglyconate. All horses were euthanatized on day 30. Intra-abdominal adhesions were graded (0-4), and stomal areas were calculated from contrast radiographs made with the bowel distended. Histopathology scores for the anastomoses were based on the degree of inflammation (0-3), fibrosis (0-3), and alignment and healing of intestinal layers (0-3). Mean surgery times +/- standard deviations for EE, SSI, and SSNI techniques were 33.9 +/- 5.4, 36.2 +/- 5.6, and 29.6 +/- 5.9 minutes, respectively. Mean and median stomal areas were 9.4 +/- 5.5 and 8.9 cm2 for EE anastomoses and 17.2 +/- 16.5 and 12.1 cm2 for SS anastomoses. Intra-abdominal adhesions developed in three horses, one of which had clinical signs of colic. Mean histopathology scores for EE and SS anastomoses were 4.8 +/- 2.0 (median = 5) and 4.4 +/- 1.8 (median = 4), respectively. There were no statistically significant differences in surgery times, intra-abdominal adhesion scores, stomal areas, or histopathology scores between small intestinal EE and SS anastomoses in these horses.  相似文献   

Granular cell tumour in the bronchus of a horse     

LM GOODCHILD  AJ DART  MB COLLINS  DR HODGSON 《Australian veterinary journal》1997,75(1):16-18

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Radiographic Progression of Osteoarthritis of the Canine Stifle Joint: A Prospective Study     

J. F. Innes  BVSc  PhD    M. Costello  MVB    F. J. Barr  MA  Vet.  MB    H. Rudorf  Dr. Med. Vet.    A. R. S. Barr  MA  Vet.  MB  PhD 《Veterinary radiology & ultrasound》2004,45(2):143-148

A radiographic scoring system for features of osteoarthritis of the canine stifle joint was devised. Intra- and inter-rater agreement was assessed by calculation of unweighted kappa statistics. "Global score," "effusion," "osteophytosis," and "intra-articular mineralization" were all features that had acceptable reliability, but "subchondral sclerosis" did not. The scoring system was used in a 13-month prospective study of the radiographic progression of osteoarthritis of the stifle joint, secondary to cranial cruciate ligament deficiency, in 58 dogs. In the index stifle joints all features were characterized by significant change over time, but osteophytosis had the greatest degree of change. In addition, 40% of contralateral joints showed progressive osteophytosis.  相似文献