Imatinib mesylate treatment in a dog with gastrointestinal stromal tumors with a c-kit mutation     

Mitsuhiro IRIE  Yoshinori TAKEUCHI  Yuzo OHTAKE  Hitomi SUZUKI  Nao NAGATA  Takuma MIYOSHI  Yumiko KAGAWA  Tetsushi YAMAGAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1535-1539

A 13-year-old spayed mixed-breed dog was diagnosed with a gastrointestinal stromal tumor (GIST) after histopathological examination of an abdominal mass. Five months after surgical resection of the tumor, we detected the recurrence of GIST with multiple disseminated abdominal lesions. A sequence analysis of cDNA obtained from a biopsy of the recurrent tumors revealed a mutation within exon 9 of the c-kit gene (1523A>T, Asn⁵⁰⁸Ile), which has been shown to cause ligand-independent phosphorylation of the KIT protein in GISTs and canine mast cell tumors (MCTs). Upon detection of the recurrent tumors, we initiated treatment with imatinib mesylate (10 mg/kg, q 24 hr). After 2 months, the dog achieved complete remission. Our findings indicate that canine GIST, and possibly MCT, may be responsive to molecular-targeted therapy.  相似文献   

Detection of rickettsial DNA in ticks and wild boars in Kyoto City, Japan     

Azusa SOMEYA  Ryuki ITO  Akihiko MAEDA  Mitsuhiro IKENAGA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):37-43

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Effect of oral administration of L-pipecolic acid on food intake in chicks and mice     

Tomo TAKAGI  Ryuichi ANDO  Atsushi OHGUSHI  Tomonori NAKANISHI  Hazizul HUSSAIN-YUSUF  Ryoji ONODERA  Hiroyuki SATO  D. Michael DENBOW  Mitsuhiro FURUSE 《Animal Science Journal》2003,74(2):101-104

It has been demonstrated that L-pipecolic acid (L-PA), a major metabolic intermediate of L-lysine (L-Lys) in the brain, is involved in the functioning of the γ-aminobutyric acid-ergic system. Previous study has shown that intracerebroventricular injection of L-PA suppressed feeding and induced sleep-like behavior in neonatal chicks. The present study examines whether the action of L-PA was induced by gavage in both chicks and mice. Oral administration of L-PA significantly inhibited food intake at 2 h after treatment in neonatal chicks, although no significant effect of L-Lys was detected. In mice, oral L-PA suppressed food intake compared to the control after 2 h of treatment. It was concluded that L-PA was effective for suppression of food intake after oral administration in both avian and mammalian species.  相似文献   

展望21世纪的世界森林   总被引：1，自引：0，他引：1  

和爱军  箕轮光博 《中南林业调查规划》2001,20(1):20-24

人类即将跨入21世纪。回顾20世纪的经济发展和工业革命历程,人们不断地进行着总结和深刻的反思。随世界范围内日益突出的地球环境问题,作为陆地上最大生态系统的森林越来越倍受关注。人们开始意识到“森林是21世纪人类自身最后的生命线”。为此,20世纪结束前夕,有关森林和可持续发展的全球性或区域性的国际组织不断产生和发展,并经常召开国际会议,讨论共同关心的问题。其中最具代表性的森林与可持续发展国际委员会（WCFSD）认为：为了拯救森林,为了使全世界仅有的森林在下世纪得到生存和发展,有必要进行根本性的变革。  相似文献   

Detection of zearalenone and its metabolites in naturally contaminated porcine follicular fluid by using liquid chromatography-tandem mass spectrometry     

Sambuu R  Takagi M  Shiga S  Uno S  Kokushi E  Namula Z  Otoi T  Miyamoto A  Deguchi E  Fink-Gremmels J 《The Journal of reproduction and development》2011,57(2):303-306

Zearalenone (ZEN) and its metabolites are important nonsteroidal estrogenic mycotoxins that cause reproductive disorders in domestic animals, especially pigs. We aimed to simultaneously detect ZEN and its metabolites á-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) in porcine follicular fluid (FF) by liquid chromatography-tandem mass spectrometry. ZEN and α-ZOL, but not β-ZOL, were detected in all pooled FF samples collected from coexisting follicles (diameter ≥ 6 mm) within 10 ovaries. Furthermore, ZEN and α-ZOL were detected in samples pretreated with β-glucuronidase/arylsulfatase, but not in those left untreated, suggesting that the FF samples contained glucuronide-conjugated forms of the mycotoxins that may be less harmful to porcine oocytes due to glucuronidation affecting the receptor binding. Nonetheless, the effects of the glucuronide-conjugated forms should be studied, both in vitro and in vivo.  相似文献   

Acute colic possibly caused by phytobezoar derived from napier grass in 5 Japanese black cows reared in the same farm     

Hasunuma H  Matsumoto D  Kirino Y  Sasa T  Fushimi Y  Ikeda T  Tshering C  Takagi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1093-1096

For 6 years, 5 Japanese Black cows of the same herd showed anorexia, depression, and dehydration with no feces in the rectum. Biomedical examination of 3 animals showed severe hypokalemia and hypochloremia. Although the first 3 animals died or were slaughtered (causes unknown), necropsy results showed that the cow in case 4 had intestinal obstruction due to phytobezoar derived from napier grass, fed mainly to the cattle as roughage. Therefore, farmers were recommended to avoid the hard root-stem portion of napier grass as roughage. Consequently, less phytobezoar was recovered from the fifth cow, and no similar clinical case of intestinal obstruction was observed thereafter. This is the first report on intestinal obstruction caused by phytobezoars derived from napier grass.  相似文献   

Intracerebroventricular injection of L-aspartic acid and L-asparagine induces sedative effects under an acute stressful condition in neonatal chicks     

Haruka YAMANE  Mari ASECHI  Yousuke TSUNEYOSHI  Isao KURAUCHI  D. Michael DENBOW  Mitsuhiro FURUSE 《Animal Science Journal》2009,80(3):286-290

The present study was conducted to clarify the central functions of L-aspartic acid (Asp) and L-asparagine (Asn) during an acute stressful condition in chicks. Intracerebroventricular (i.c.v.) injection of Asp and Asn (0.84 µmol) attenuated the vocalization that normally occurs during social separation stress. Asp decreased the time spent in active wakefulness and induced sedation. Asn had a similar effect to Asp, although somewhat weaker. However, i.c.v. injection of Asp and Asn further enhanced plasma corticosterone release under social separation stress. Taken together, the i.c.v. injection of Asp and Asn has sedative effects under an acute stressful condition, which does not involve the hypothalamic-pituitary-adrenal axis.  相似文献   

Studies on soils of Peaty paddy fields I     

Yasuo Takijima  Mitsuhiro Shiojima  Kiichi Konno 《Soil Science and Plant Nutrition》2013,59(2):83-92

Rice is the main crop grown in Japan, approximately three million hectares or half of the total arable land being planted to rice. However, not less than 20 percent of the rice fields is estimated of so called “Akiochi” type, which means autumn-decline in English. It has been observed that these low productive paddy soils may be divided roughly into two types, one of which is the soil of well-drained, so called “degraded” paddy field, and the other is the soil of ill-drained one with too much organic matter, humus, in the soil.  相似文献   

Isolation of a novel mutant gene for soil-surface rooting in rice (Oryza sativa L.)     

Eiko Hanzawa  Kazuhiro Sasaki  Shinsei Nagai  Mitsuhiro Obara  Yoshimichi Fukuta  Yusaku Uga  Akio Miyao  Hirohiko Hirochika  Atsushi Higashitani  Masahiko Maekawa  Tadashi Sato 《Rice》2013,6(1):30

Background

Root system architecture is an important trait affecting the uptake of nutrients and water by crops. Shallower root systems preferentially take up nutrients from the topsoil and help avoid unfavorable environments in deeper soil layers. We have found a soil-surface rooting mutant from an M₂ population that was regenerated from seed calli of a japonica rice cultivar, Nipponbare. In this study, we examined the genetic and physiological characteristics of this mutant.

Results

The primary roots of the mutant showed no gravitropic response from the seedling stage on, whereas the gravitropic response of the shoots was normal. Segregation analyses by using an F₂ population derived from a cross between the soil-surface rooting mutant and wild-type Nipponbare indicated that the trait was controlled by a single recessive gene, designated as sor1. Fine mapping by using an F₂ population derived from a cross between the mutant and an indica rice cultivar, Kasalath, revealed that sor1 was located within a 136-kb region between the simple sequence repeat markers RM16254 and 2935-6 on the terminal region of the short arm of chromosome 4, where 13 putative open reading frames (ORFs) were found. We sequenced these ORFs and detected a 33-bp deletion in one of them, Os04g0101800. Transgenic plants of the mutant transformed with the genomic fragment carrying the Os04g0101800 sequence from Nipponbare showed normal gravitropic responses and no soil-surface rooting.

Conclusion

These results suggest that sor1, a rice mutant causing soil-surface rooting and altered root gravitropic response, is allelic to Os04g0101800, and that a 33-bp deletion in the coding region of this gene causes the mutant phenotypes.  相似文献   

Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury     

Hideaki OHTSUBO  Wataru MIZUNOYA  Mako NAKAMURA  Mitsuhiro FURUSE  Yoshihide IKEUCHI  Ryuichi TATSUMI 《Animal Science Journal》2013,84(2):185-189

Regenerative mechanisms that regulate intramuscular motor innervation. including configuration of the neuromuscular connections are thought to reside in the spatiotemporal expression of axon‐guidance molecules. Our previous studies proposed a heretofore unexplored role of satellite cells as a key source of a secreted neural chemorepellent semaphorin 3A (Sema3A) expression. In order to verify this concept, there is still a critical need to provide direct evidence to show the up‐regulation of Sema3A protein in satellite cells in vivo upon muscle injury. The present study employed a Sema3A/MyoD double‐immunohistochemical staining for cryo‐sections prepared from cardiotoxin injected gastrocnemius muscle of adult mouse lower hind‐limb. Results clearly demonstrated that Sema3A expression was up‐regulated in myogenic differentiation‐positive satellite cells at 4–12 days post‐injury period, the time that corresponds to the cell differentiation phase characterized by increasing myogenin messenger RNA expression. This direct proof encourages a possible implication of satellite cells in the spatiotemporal regulation of extracellular Sema3A concentrations, which potentially ensures coordinating a delay in neurite sprouting and re‐attachment of motoneuron terminals onto damaged muscle fibers early in muscle regeneration in synchrony with recovery of muscle‐fiber integrity.  相似文献