Antimicrobial and bactericidal activities of esters of 2-endo-hydroxy-1,8-cineole as new aroma chemicals     

Miyazawa M  Hashimoto Y 《Journal of agricultural and food chemistry》2002,50(12):3522-3526

Fourteen kinds of alkyl esters of 2-endo-hydroxy-1,8-cineole were synthesized, with yields of 57.8-98.0%. Each ester had a characteristic and unique odor. Especially, the tert-butyl acetate of 2-endo-hydroxy-1,8-cineole had the most interesting odor of all the synthetic esters. The antimicrobial and bactericidal activities of these synthetic esters against test bacteria (Staphylococcus aureus, Escherichia coli, and Pseudomonas fluorescens) were examined using the broth dilution method. As a result, the tert-butyl acetate of 2-endo-hydroxy-1,8-cineole showed the highest antimicrobial and bactericidal activities against all kinds of the test bacteria.  相似文献   

Larvicidal and adulticidal activity of alkylphthalide derivatives from rhizome of Cnidium officinale against Drosophila melanogaster   总被引：1，自引：0，他引：1  

Tsukamoto T  Ishikawa Y  Miyazawa M 《Journal of agricultural and food chemistry》2005,53(14):5549-5553

The insecticidal activity of the chloroform extract of Cnidium officinale rhizomes and its constituents was investigated against larvae and adults of Drosophila melanogaster and compared with that of rotenone. Bioassay-guided isolation of the chloroform extract of C. officinale resulted in the isolation and characterization of four alkylphthalides, cnidilide (1), (Z)-ligustilide (2), (3S)-butylphthalide (3), and neocnidilide (4). The structures of these compounds were established by spectroscopic analysis. The isolated compounds 2, 3, and 4 exhibited LC50 values of 2.54, 4.99, and 9.90 micromol/mL of diet concentration against larvae of D. melanogaster, respectively. Against both sexes (males/females, 1:1) of adults (5-7 days old), compound 3 showed the most potent activity of the compounds isolated with the LD50 value of 5.93 microg/adult, comparable to that of rotenone (LD50 = 3.68 microg/adult). Structure-activity relationships of phthalides isolated suggest that the presence of conjugation with the carbonyl group in the lactone ring appeared to play an important role in the larvicidal activity. Acetylcholinesterase (prepared from the adult heads of D. melanogaster) inhibitory activity was also investigated in vitro to determine the insecticide mode of action for the acute adulticidal activity.  相似文献   

Detection of calmodulin and calmodulin-binding proteins in pure phloem sap of rice plants     

Shin-ichi NAKAMURA  Hiroaki HAYASHI  Mitsuo CHINO 《Soil Science and Plant Nutrition》2006,52(2):195-202

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Optimization of Cellulose Extraction from Jute Fiber by Box-behnken Design     

Lizandro Manzato  Mitsuo Lopes Takeno  Wanison André Gil Pessoa-Junior  Luis André Morais Mariuba  John Simonsen 《Fibers and Polymers》2018,19(2):289-296

Cellulose was isolated from plant material for the first time in 1839 by the French chemist Anselme Payen. In recent years, due to the need in reduce the world’s environmental problems, there has been an increase in studies related to the physical and chemical factors of cellulose. It is important to emphasize that experiments and studies with a cellulose occur individually, because of the variation in the amount of cellulose and the extraction method that differs from plant to plant. In the present study, we determined the optimal conditions for cellulose extraction of jute fiber, using the response surface method. The Box-Behnken Design (BBD) was used statistically evaluate the ratio effects of sodium hydroxide (NaOH) and sodium hypochlorite (NaClO), temperature and extraction time in the process used. The analysis of the results showed a significant variable in the linear and quadratic terms of the temperature and also a significant level of interaction in the effect between the variables of temperature and time. Besides this, the BBD used for the analysis of the extraction yield, resulted in a polynomial regression of second order, in complete agreement with experimental results, with R²=0.9627 (p<0.05). The optimal condition was obtained in a ratio of 1.3 at 45 °C for 2 h. Under the best possible conditions, the obtained experimental value is in accordance with the value predicted by the model, thus indicating a model combination and success to optimize the extraction conditions of the jute fiber pulp in the response surface methodology.  相似文献   

Spawning frequency of the Tsushima Current subpopulation of chub mackerel <Emphasis Type="Italic">Scomber japonicus</Emphasis> off Kyushu,Japan     

Tetsuro Shiraishi  Suvarna D. Ketkar  Yoshiki Katoh  Mitsuo Nyuji  Akihiko Yamaguchi  Michiya Matsuyama 《Fisheries Science》2009,75(3):649-655

Female Japanese chub mackerel Scomber japonicus of the Tsushima Current subpopulation were collected during the spawning season from March to May 2001. A total of 137 adult females were caught between midnight and daybreak. A considerable number of fish displayed new postovulatory follicles (POF), whereas there was no evidence of germinal vesicle breakdown or hydrated oocytes in any of the fish collected. This suggests a daily spawning synchronicity toward midnight. To estimate the spawning frequency (S), the female reproductive state was classified into four criteria based on the degenerative stage of the POFs and the developmental stage of the oocytes. To stage the POFs according to age and determine the stage duration, ovaries from S. japonicus were induced to spawn in the laboratory and were sampled 0–72 h after ovulation at appropriate intervals. The average S, which is evaluated from four different indices, was 16.9%, corresponding to the average female chub mackerel spawned every 5.9 days (8.5 times) during the 50 days.  相似文献