Nocturnal/diurnal demand-feeding pattern of yellowtail Seriola quinqueradiata under different keeping conditions   总被引：1，自引：0，他引：1  

Jun Kohbara  Iwao Hidaka  Isao Kuriyama  Mitsushi Yamashita  Masasuke Ichikawa  Kiyoshi Furukawa  Katsumi Aida  F. Javier Sánchez-Vázquez and  Mitsuo Tabata 《Fisheries Science》2000,66(5):955-962

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A quantitative study on arginine catabolism by mixed ruminal bacteria,protozoa and their mixture in vitro     

Halima SULTANA  Hazizul HUSSAIN‐YUSUF  Toshihiro TAKAHASHI  Tetsuo MORITA  Ryoji ONODERA 《Animal Science Journal》2003,74(1):11-16

The catabolism of arginine (Arg) by mixed rumen bacteria (B), mixed rumen protozoa (P), and their mixture (BP) was quantitatively investigated in an in vitro system in order to confirm the metabolic pathway of Arg and provide basic information for enzymatic and molecular studies as well as an understanding of the quantitative distribution of metabolites. Rumen microbial suspensions (B, P, and BP) collected from fistulated goats were anaerobically incubated with or without 1 mmol/L Arg at 39°C for 12 h. Arg and other related compounds such as citrulline (Cit), ornithine (Orn), proline (Pro) and 5‐aminovaleric acid (5AV) in both supernatant and hydrolyzates of B, P, and BP suspensions were analyzed by HPLC. The metabolic pathways of Arg in mixed rumen bacteria and mixed rumen protozoa were considered to be as follows: rumen bacteria, Arg → Cit → Orn → Pro → 5AV → VFAs + NH₃; rumen protozoa, Arg → Cit → Orn → Pro → 5AV. The disappearance of Arg (1 mmol/L) was approximately 52.9 and 88.2% in B, 33.9 and 55.6% in P, and 52.8 and 85.2% in BP during 6 and 12 h incubations, respectively. When expressed in units of ‘per gram (g) of microbial nitrogen (MN)’, the net degradation rate of Arg in BP (50.3 µmol/g MN/h) was approximately 46% lower than that of B during a 12 h incubation period. The presence of protozoa tended to inhibit the production of Orn from Cit and the production of 5AV from Pro which were thought to be rate‐limiting steps of Arg metabolism in rumen microorganisms. As a result, protozoa appeared to have a saving effect on Arg metabolism, that is, protozoa protected Arg from wasteful exhaustion in the rumen.  相似文献   

Effect of oral administration of 3,3',4,4',5-pentachlorobiphyenl on the intestinal microbiota of Sprague–Dawley rats     

Kaoru YAMAZAKI  Takehito SUZUKI  Mitsuyuki SHIRAI  Tatsuya TAKIZAWA  Tadashi SHINODA  Toshio MASAOKA  Fumiaki AKAHORI  Hidetoshi MORITA 《Animal Science Journal》2008,79(3):391-400

The effect of the endocrine-disrupting chemical 3,3',4,4',5-pentachlorobiphyenl (PCB 126) on intestinal microbiota after oral administration, and the improvement of intestinal microbiota and feces quantity by the subsequent administration of Lactobacillus acidophilus or Lactobacillus reuteri was investigated. All the rats were given 100 μg/kg bodyweight of PCB 126. The changes in bacterial counts were confirmed using a culture method. The administration of PCB 126 tended to decrease the bacterial counts of lactobacilli (10^9.6−10^10.2 to 10^8.8−10^9.2) and bifidobacteria (10^5.3−10^6.1 to 10^3.6−10^4.2), and to increase those of Enterobacteriaceae (10^8.2−10^9.1 to 10^9.4−10^10.3) and staphylococci (10^6.6−10^7.4 to 10^7.2−10^8.4) compared to no PCB 126 administration. After administration of PCB 126, L. acidophilus or L. reuteri orally administered to rats caused Enterobacteriaceae and staphylococci counts to decrease, suggesting that the intestinal microbiota was improved by the lactobacilli. The administration of L. acidophilus and L. reuteri improved the balance of intestinal microbiota, and defecation volume returned to its normal level. L. acidophilus and L. reuteri have a remedial effect on intestinal microbiota affected by PCB 126 and can function to lessen accumulated PCB 126 volume.  相似文献   

Genetic evaluation of the longevity of the Holstein population in Japan using a Weibull proportional hazard model     

Osamu SASAKI  Mitsuo AIHARA  Koichi HAGIYA  Akiko NISHIURA  Kazuo ISHII  Masahiro SATOH 《Animal Science Journal》2012,83(2):95-102

The objective of this study was to confirm the stability of the genetic estimation of longevity of the Holstein population in Japan. Data on the first 10 lactation periods were obtained from the Livestock Improvement Association of Japan. Longevity was defined as the number of days from first calving until culling or censoring. DATA1 and DATA2 included the survival records for the periods 1991–2003 and 1991–2005, respectively. The proportional hazard model included the effects of the region-parity-lactation stage-milk yield class, age at first calving, the herd–year–season, and sire. The heritabilities on an original scale of DATA1 and DATA2 were 0.119 and 0.123, respectively. The estimated transmitting abilities (ETAs) of young sires in DATA1 may have been underestimated, but coefficient δ, which indicated the bias of genetic trend between DATA1 and DATA2, was not significant. The regression coefficient of ETAs between DATA1 and DATA2 was very close to 1. The proportional hazard model could steadily estimate the ETA for longevity of the sires in Japan.  相似文献   

Condensation reactions of phenolic resins VII: catalytic effect of sodium bicarbonate for the condensation of hydroxymethylphenols     

Naruyuki Kamo  Jun Tanaka  Mitsuo Higuchi  Tetsuo Kondo  Mitsuhiro Morita 《Journal of Wood Science》2006,52(4):325-330

This article describes the catalytic effect of NaHCO₃ on condensation reactions of monomeric hydroxymethylphenols (HMPs) to elucidate the cure-acceleration mechanism. By comparison of the kinetics of self-condensations of HMPs, NaHCO₃ was proved to increase the reactivity of para-hydroxymethyl groups. The changes of ¹³C nuclear magnetic resonance (NMR) chemical shifts on each HMP system with the additive indicated that the addition of NaHCO₃ enhanced some molecular interactions between HMPs and NaHCO₃, facilitating a resonance effect that might play a similar role in dissociation of the phenolic hydroxyl groups of HMPs. In addition, computational modeling by molecular orbital calculations elucidated that hydrogen carbonate anion (HCO ₃ ⁻ ) forms an interaction between either the para-hydroxymethyl group and the phenolic hydroxyl group or between the two para-hydroxymethyl groups of HMPs by hydrogen bonds. From the experimental results, the authors proposed the mechanism of the catalytic action of NaHCO₃: it appears to be due to the delocalization of an electron initiated by the interaction of the para-hydroxymethyl groups and the phenolic hydroxyl of HMPs with HCO ₃ ⁻ through hydrogen bonds, which results in facilitating the formation of active species.  相似文献   

Complex vertebral malformation in a stillborn Holstein calf in Japan     

Nagahata H  Oota H  Nitanai A  Oikawa S  Higuchi H  Nakade T  Kurosawa T  Morita M  Ogawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1107-1112

A female stillborn Holstein calf with shortened cervical and thoracic regions, protrusion of the tongue, and bilateral symmetric flexural contraction of the anterior limbs was delivered on gestation day 281. Multiple hemivertebrae, fused and misshaped vertebrae, synostosis and scoliosis of cervical, thoracic and lumbar vertebral column were found in the affected calf by radiographic and computed tomographic (CT) analysis. Ten pairs of ribs were present and the sternum consisted of 9 sternebrae. Multiple morphologic abnormalities including fusion, malformation, and displacement, were found in the ribs and sternum. Cardiac anomalies, including atrial septal defect and hypertrophy of right ventricle, were observed. DNA-polymerase chain reaction (PCR) analysis demonstrated that amplified product from the liver DNA of the affected calf had identical pattern to that associated with complex vertebral malformation (CVM) of Holstein calves and that her dam was a heterozygous carrier of CVM. The affected calf was diagnosed as having CVM based on the DNA-PCR results and the characteristic findings, and was recorded as a first documentation of CVM confirmed in a Holstein calf in Japan.  相似文献   

Genetic and serological characterization of novel serotype G8 bovine group A rotavirus strains isolated in Japan     

Fukai K  Onoda H  Itou T  Sato M  Miura Y  Sakai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1413-1416

G8 bovine group A rotaviruses isolated in Japan were genetically and serologically characterized. The VP7 gene nucleotide and amino acid sequences revealed high identity with each other. All Japanese G8 strains were classified into the same lineage in the phylogenetic analysis based on VP7 gene sequences. Antisera to four Japanese G8 strains neutralized other G8 strains, but their neutralizing titers were between 8-fold lower and 2-fold higher than homologous strains. These results suggest that the VP7s of Japanese G8 strains have similar genetic and serologic characteristics. Observed differences in the neutralizing abilities of antisera for each strain appear to depend on differences in the P serotypes/genotypes.  相似文献   

Comparative Analysis of Japanese and Foreign Strains of <Emphasis Type="Italic">Ralstonia solanacearum</Emphasis> Based on 16S Ribosomal RNA Gene Sequences     

Mitsuo HORITA  Kenichi TSUCHIYA 《Journal of General Plant Pathology》2000,66(2):132-137

We determined nearly the complete sequences of the 16S ribosomal RNA gene (rDNA) for Japanese strains of R. solanacearum. The comparison of 1471 nucleotide positions separated the Japanese strains into two groups, group 1 with biovars 1, 2, 3 and 4 strains which belonged to race 1, and group 2 with biovar 2 strains corresponding to race 3. Group 1 strains all had identical sequences, and strains representing the four biovars within the group did not differ from each other. Group 2 strains had characteristic nucleotides which differed at seven positions from group 1 strains. Comparative analysis of Japanese and foreign strains based on 16S rDNA sequences showed that Japanese group 1 was closely related to Asian and Australian biovars 3, 4 and 5, and belonged to the known division 1. Japanese group 2 was homogeneous to Indonesian biovars 2 and N2 in subdivision 2b. Since the differences in the nucleotides corresponded to restriction sites for the AluI, RFLP analysis of PCR-amplified 16S rDNA efficiently differentiated not only Japanese group 1 from group 2, but also differentiated three types of foreign strains which differed in biovar and geographic origin. Received 26 July 1999/ Accepted in revised form 19 November 1999  相似文献   

弱光照射和无机营养供给对冷藏绿芦笋品质变化的影响   总被引：9，自引：0，他引：9  

顾振新  饭本光雄  田川彰南  李式军 《南京农业大学学报》2001,24(4):84-88

绿芦笋嫩茎基部切口插入含有N、P、K营养的琼脂凝胶中 ,在 (6± 0 2 )℃下贮藏。贮藏期间 ,分别以有效光量子束密度 (PPFD)为 2 μmol·m-2 ·s-1的红色发光二极管和白色荧光灯作为弱光源 ,每天 2 4h连续照射绿芦笋 2 1d。结果表明 ,红光和白光对冷藏绿芦笋品质的保持具有相似的效果。供给无机营养的处理在红光或白光照射下 ,色值 (a 和ΔE ab)变化率、粗纤维含量增长率和可溶性固形物减少率为最低 ,其次是无营养供给的红光或白光处理 ,以黑暗处理为最高 ;供给无机营养处理的芦笋嫩茎伸长率大于无营养处理的 ;光线照射与否和无机营养供给有无与芦笋失重率之间无明显相关性  相似文献   

Effect of chloride application and low soil pH on cadmium uptake from soil by plants   总被引：3，自引：0，他引：3  

Hiroyuki HATTORI  Kouki KUNIYASU  Kaori CHIBA  Mitsuo CHINO 《Soil Science and Plant Nutrition》2006,52(1):89-94

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