A study of the distribution of color Doppler flows in the superficial digital flexor tendon of young Thoroughbreds during their training periods     

Takashi HATAZOE  Yoshiro ENDO  Yohei IWAMOTO  Kenji KOROSUE  Taisuke KURODA  Saemi INOUE  Daiki MURATA  Seiji HOBO  Kazuhiro MISUMI 《Journal of Equine Science》2015,26(4):99-104

  相似文献   

Clinical and pathologic features of neuronal ceroid-lipofuscinosis in a ferret (Mustela putorius furo)     

Nibe K  Miwa Y  Matsunaga S  Chambers JK  Uetsuka K  Nakayama H  Uchida K 《Veterinary pathology》2011,48(6):1185-1189

Clinical and pathologic features of neuronal ceroid-lipofuscinosis in a 4-month-old ferret are reported. Clinical signs including neurological symptoms appeared at 3 months of age and progressed rapidly. By magnetic resonance imaging, severe cerebral atrophy was recognized. Histopathologically, there was severe neuronal loss and diffuse astrogliosis with macrophage accumulations; lesions were found predominantly in the cerebral cortex. Intracytoplasmic pigments were observed in surviving neurons and macrophages throughout the brain. The pigments were intensely positive for periodic acid-Schiff, Luxol fast blue, and Sudan black B and exhibited a green autofluorescence. Electron microscopic examination revealed the accumulation of electron-dense granular material within lysosomes of neurons and macrophages. Immunohistochemically, a large number of saposin-positive granules accumulated in the neuronal cells, astrocytes, and macrophages of the lesions, but significant immunoreactivity for subunit c of mitochondrial adenosine triphosphate synthase was not observed. Based on these findings, the animal was diagnosed as affected by neuronal ceroid-lipofuscinosis.  相似文献   

A 4-week Repeated Dose Toxicity Study of Glycine in Rats by Gavage Administration     

Yusuke Shibui  Tadashi Miwa  Mayumi Yamashita  Keigi Chin  Terutaka Kodama 《Journal of toxicologic pathology》2013,26(4):405-412

In order to examine the toxicity profile of glycine, an authorized food additive, a solution of glycine in water for injection was administered orally (via gavage) to male SD rats (Crl:CD(SD)) once daily for 4 weeks at doses of 500, 1000 and 2000 mg/kg/day in a volume of 10 mL/kg. Control animals received vehicle only. No animals died, and no glycine-related changes were observed in body weight, food consumption, water consumption, hematology, organ weight, gross pathological examination or histopathological examination. In urinalysis, daily urinary volume and urinary Cl excretion were significantly higher in the 2000 mg/kg/day dose group, and urine pH and urinary protein showed lower trends in the glycine-treated groups. However, these changes were considered to be of little toxicological significance, because there were no histopathological changes in the kidneys or urinary bladder and no changes in other urinary parameters. As regards blood chemistry, phospholipids were significantly higher in the 2000 mg/kg/day dose group. However, the increase was small and was not considered to be toxicologically significant. In conclusion, none of the animals in any of the glycine-treated groups showed changes that were considered toxicologically significant. Therefore, the no-observed-adverse-effect level of glycine was estimated to be at least 2000 mg/kg/day under the conditions of this study.  相似文献   

Changes in the levels of mRNA coding for gonadotropin Iβ and IIβ subunits during vitellogenesis in the common Japanese conger Conger myriaster     

Shingo Kajimura  Yasutoshi Yoshiura  Miwa Suzuki  Tomoko Utoh  Noriyuki Horie  Hideo Oka  Katsumi Aida 《Fisheries Science》2001,67(6):1053-1062

  相似文献   

Assessment of meibomian gland morphology by noncontact infrared meibography in Shih Tzu dogs with or without keratoconjunctivitis sicca     

Yasunari Kitamura  Seiya Maehara  Tetsuya Nakade  Yukihiro Miwa  Reiko Arita  Hiroko Iwashita  Akihiko Saito 《Veterinary ophthalmology》2019,22(6):744-750

  相似文献   

Immunohistochemical and scanning electron microscopic comparison of the collagen network constructions between pig, goat and chicken livers     

Shotaro NISHIMURA  Ayano SAGARA  Ichiro OSHIMA  Yoshitaka ONO  Hisao IWAMOTO  Kaoru OKANO  Hideyuki MIYACHI  Shoji TABATA 《Animal Science Journal》2009,80(4):451-459

The distribution and three-dimensional architecture of collagen fibers were compared between pig, goat and chicken livers. Immunohistochemical staining revealed that collagen type I was identified in the interlobular connective tissue region and intralobular areas in pigs and goats. Type III collagen was also identified in the interlobular connective tissue region and intralobular sinusoidal walls. In the chicken liver, only the circumference region of the vessels was immunostained with collagen type I and III antibodies and the interlobular connective tissue wall could not be distinguished clearly. In the intralobular region, collagen type I antibody immunoreacted around the hepatic cells but collagen type III antibody immunoreacted weakly. In the NaOH macerated specimen, well-developed collagen bundles formed the prominent interlobular walls in pigs. In contrast, the wall in the goat liver comprised a thin layer of the bundles. In the chicken liver, there were no notable collagen septa between lobules. The intralobular collagen construction was quite different between the animals, indicating a fragile collagen fibril networks in pigs, a robust framework in goats and dense fabric-like septa in chickens. These results indicate that the distinct collagen frameworks may contribute to the histological strength of the livers in each of the animal species.  相似文献   

Phylogenetic analysis of Theileria sp. from sika deer, Cervus nippon, in Japan     

Inokuma H  Tsuji M  Kim SJ  Fujimoto T  Nagata M  Hosoi E  Arai S  Ishihara C  Okuda M 《Veterinary parasitology》2004,120(4):339-345

The 18S rRNA genes of Theileria species detected in sika deer, Cervus nippon centralis in Yamaguchi and Cervus nippon yesoensis in Hokkaido, were analyzed. The percent identities of the nucleotide sequences of Theileria from Cervus nippon centralis and Cervus nippon yesoensis were more than 99%. The percent identities of the Theileria sp. from sika deer and Theileria sergenti, Theileria buffeli and Theileria cervi were 97, 96 and 95%, respectively. Phylogenetic analysis of the gene sequences also revealed that Theileria sp. detected from sika deer comprise a clade that is clearly distinct from the clade comprised of Theileria from cattle.  相似文献   

DIFFERENCES IN ACQUISITION OF ORGANIC NITROGEN IN SOILS BETWEEN BOK CHOY AND TOMATO     

Toshihiro Watanabe  Takumi Enomoto  Michihiko Sakurai  Miwa Okamoto  Takuro Shinano  Mitsuru Osaki 《Journal of plant nutrition》2013,36(10):1468-1476

Seedlings of bok choy and tomato were grown in soils with different nitrogen (N) sources [no N (?N), ammonium sulfate (AS), and cattle farmyard manure (CM)]. Comparison between soils treated with ?N and CM indicated that the growth and N accumulation in bok choy were significantly enhanced by CM treatment, whereas no difference was found in tomato. In the rhizosphere soils, the highest protease activity was detected in CM treatment irrespective of species. Correlation analysis between rhizospheric protease activity and total N accumulation of plant treated with –N and CM showed a significant positive correlation only for bok choy. The determination of amino acid absorption rate in excised roots indicated that glycine was taken up at a significantly higher rate in bok choy than tomato. This study suggested that at least two possible factors affected the acquisition of organic N: rhizospheric protease activity and ability to absorb amino acids in roots.  相似文献   

Effects of peat moss and sawdust compost applications on N2O emission and N leaching in blueberry cultivating soils     

Imre Vano  Miwa Matsushima  Changyuan Tang 《Soil Science and Plant Nutrition》2013,59(2):348-360

  相似文献   

Development and age‐related change of cerebrovascular noradrenergic (NA) innervation in the Japanese quail     

Kiyonori HAYASHI  Mitsuhiro FURUSE  Tomoki SOH  Haruo KUSABA  Hisao IWAMOTO  K ichi AND 《Animal Science Journal》2002,73(6):523-531

Development of cerebrovascular noradrenergic (NA) innervation was investigated in the Japanese quail, using histofluorescence technique and quantitative analysis. Cerebral perivascular NA nerves arose from the cerebral carotid (CCA), internal ethmoidal (IEA) and vertebral (VA) arteries before hatching, with the first appearance on the CCA and IEA at embryonic day (E) 8 and of the VA at E14. Nerves arising from the IEA were greater in number and spread more rapidly than those from the CCA and VA. On E16, fluorescent fiber bundles lying on the anterior circulation subdivided rapidly into thin fibers. Consequently, the number of NA nerves over the arterial system increased greatly at hatching, particularly along the distal portion of the anterior ramus. At the same developmental stage, all the major arteries of the anterior and posterior circulation were almost entirely covered by NA nerves. The abrupt reorganization of cerebrovascular NA innervation in newborn quails may be related to some specific trophic and vasomotor roles for structural and functional improvement of the cerebral circulation that is required for its brain differentiation at this critical period of development. The supply of NA nerves to the anterior and posterior circulations sequentially increased during development from posthatching day (P) 1–15. Plexuses of NA nerves in each of the major cerebral arteries at P22 and P42–50 were similar in meshwork construction to each other, and to those seen at P15. Likewise, there was no clear statistical difference in the nerve density of the corresponding arteries among the three posthatching ages, except for the caudal basilar artery.  相似文献