Induction of proinflammatory cytokines and caspase-1 by leptin in monocyte/macrophages from holstein cows   总被引：3，自引：0，他引：3  

Ahmed M  Shaban Z  Yamaji D  Okamatsu-Ogura Y  Soliman M  Abd Eldaim M  Ishioka K  Makondo K  Saito M  Kimura K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):509-514

The proliferation of peripheral blood mononuclear cells (PBMC) containing both monocyte/macrophages and T lymphocytes increased after treatment with T-cell mitogen (concanavalin A: Con A). PBMC treated with either leptin alone or combination of leptin and ConA showed enhanced proliferative activity by 10-40%, compared with those treated with ConA alone. In contrast, isolated T lymphocytes treated with leptin and ConA showed lowered proliferative activity than the ConA-treated alone, indicating that leptin induced production of some cytokines from monocyte/macrophages, that subsequently resulted in enhancement of T lymphocytes proliferation in PBMC. Among the cytokines examined, monocyte/monocytes constitutively expressed interleukin (IL)-1beta, IL-12p35, IL-18 mRNA, and faintly expressed tumor necrosis factor (TNF)-alpha and IL-12p40 mRNA. Leptin treatment augmented the monocyte/macrophages mRNA expression of only TNF-alpha and IL-12p40 to comparable levels of cells treated with lipopolysaccharide (LPS). However, leptin treatment increased monocyte/macrophages production of IL-1beta as well as TNF-alpha, and induced the mRNA expression of caspase-1, which is shown to mediate the conversion of latent pro-IL-1beta and pro-IL-18 to active forms. These results suggest that leptin directly acts on monocyte/macrophages to produce factors that induce T lymphocytes proliferation such as IL-12p35/p40 complex through IL-12p40 induction and IL-1beta/IL-18 production through caspase-1 induction.  相似文献   

A survey of rift valley fever and associated risk factors among the one-humped camel (<Emphasis Type="Italic">Camelus dromedaries</Emphasis>) in Sudan     

Maiy M. M. Abdallah  Ibrahim A. Adam  Tamadur M. Abdalla  Sanaa A. Abdelaziz  Mohamed E. Ahmed  Imadeldin E. Aradaib 《Irish veterinary journal》2015,69(1):6

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Prevalence of amyloid deposition in mature healthy chickens in the flock that previously had outbreaks of vaccine-associated amyloidosis     

Kanata IBI  Tomoaki MURAKAMI  Wael Mohamed GODA  Naoki KOBAYASHI  Naotaka ISHIGURO  Tokuma YANAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1241-1245

Avian amyloid A (AA) amyloidosis is commonly observed in adult birds with chronic inflammation, such as that caused by bacterial infection. We previously described vaccine-associated AA amyloidosis in juvenile chickens. In this study, the prevalence of amyloid deposition was measured in mature healthy chickens that survived a previous outbreak of avian AA amyloidosis while they were juveniles. Herein, we analyzed the amyloid deposition in mature chickens and compared the prevalence of amyloid deposition with juvenile chickens obtained in our previous study (Murakami et al., 2013). We found that: 1) amyloid deposition in the liver was absent in mature chickens, while juvenile chickens had a rate of 24%; 2) amyloid deposition in the spleen was observed in 36% of juvenile chickens and in 40% of mature chickens; 3) amyloid deposition in the pectoral muscle of mature chickens (43.75%) was approximately half that of juvenile chickens (88%). These results suggest that additional amyloid deposition in chickens previously exposed to AA amyloidosis may not worsen with age. Further, amyloid deposition in chickens may tend to regress when causative factors, such as vaccinations and/or chronic inflammation, are absent.  相似文献   

Impact of wheat-Leymus racemosus added chromosomes on wheat adaptation and tolerance to heat stress     

Yasir Serag Alnor Mohammed  Izzat Sidahmed Ali Tahir  Nasrein Mohamed Kamal  Amin Elsadig Eltayeb  Abdelbagi Mukhtar Ali  Hisashi Tsujimoto 《Breeding Science》2014,63(5):450-460

Adaptation of wheat (Triticum aestivum L.) to high temperatures could be improved by introducing alien genes from wild relatives. We evaluated the responses of wheat-Leymus racemosus chromosome introgression lines to high temperature to determine their potentiality for developing improved wheat cultivars. Introgression lines and their parent Chinese Spring were evaluated in a growth chamber at the seedling stage and in the field at the reproductive stage in two heat-stressed environments in Sudan. Optimum and late planting were used to ensure exposure of the plants to heat stress at the reproductive stage. The results revealed the impact of several Leymus chromosomes in improving wheat adaptation and tolerance to heat. Three lines possessed enhanced adaptation, whereas two showed high heat tolerance. Two addition lines showed a large number of kernels per spike, while one possessed high yield potential. Grain yield was correlated negatively with the heat susceptibility index, days to heading and maturity and positively with kernel number per spike and triphenyl tetrazolium chloride assay under late planting. The findings suggest that these genetic stocks could be used as a bridge to introduce the valuable Leymus traits into a superior wheat genetic background, thus helping maximize wheat yield in heat-stressed environments.  相似文献   

Physiological and biochemical responses for two cultivars of Pisum sativum (“Merveille de Kelvedon” and “Lincoln”) to iron deficiency conditions     

Nahida Jelali  Marta Dell’Orto  Mokded Rabhi  Graziano Zocchi  Chedly Abdelly  Mohamed Gharsalli 《Scientia Horticulturae》2010

The aim of this work was to compare the tolerance and the physiological responses to Fe deficiency of two Pisum sativum cultivars (“Merveille de kelvedon” and “Lincoln”) commonly cultivated in Tunisia. We studied the effects of Fe deficiency on: (i) chlorophyll content, relative growth rate (RGR) and Fe status, (ii) rhizosphere acidification, (iii) changes under Fe-deficient conditions in the activities of two root enzymes, the first related to the proton extrusion (H⁺-ATPase) and the second to iron reduction mechanism (Fe(III)-chelate-reductase: FCR). Three treatments were used: C, control, complete nutrient solution (CNS) containing 30 μM Fe; DD, direct deficiency, CNS without iron; ID, indirect deficiency, CNS containing 30 μM Fe + lime. Fe deficiency led to a significant decrease of chlorophyll content in both cultivars. The below reduction was observed in Fe-deficient plants of Merveille de Kelvedon. In addition, relative growth of shoots and whole plant was not affected by Fe deficiency. H⁺-ATPase and FCR activities were more stimulated in Merveille de Kelvedon than in Lincoln, under DD and ID Fe deficiency. The capacity of this cultivar to maintain plant growth and to preserve adequate chlorophyll synthesis under iron-limiting conditions is related to its better Fe-use efficiency, in addition to its high acidification and root reducing capacities. This allows us to suggest that Merveille de Kelvedon is more effective in overcoming Fe deficiency than Lincoln.  相似文献   

New amide alkaloid from the aerial part of Piper capense L.f. (Piperaceae)     

Ali Mohamed Kaou  Valérie Mahiou-Leddet  Cécile Canlet  Laurent Debrauwer  Sébastien Hutter  Nadine Azas  Evelyne Ollivier 《Fitoterapia》2010

Together with apigenine dimethylether and piperchabamide A, a new amide alkaloid, Kaousine and the Z form of antiepilepsirine were isolated from the aerial part of Piper capense L.f (Piperaceae). Their structures were elucidated by spectrometric methods and their in vitro antiparasitic activities were evaluated on Plasmodium falciparum.  相似文献   

Lecithin cholesterol acyltransferase (LCAT) activity as a predictor for ketosis and parturient haemoglobinuria in Egyptian water buffaloes     

Mohamed M. Ghanem  Wael M. El-Deeb 《Research in veterinary science》2010,88(1):20-25

Lecithin cholesterol acyltransferase (LCAT) activity was measured in 48 Egyptian water buffaloes four weeks pre-parturient. The activity was significantly low in 37 buffaloes (77.1%). Four weeks post-partum, clinical examination revealed that 23 buffaloes had the clinical signs of ketosis (K) while 14 had the clinical signs of parturient-haemoglobinuria (PHU). Serum samples were collected from 5 buffaloes of each group (K and PHU) besides 5 clinically healthy buffaloes with normal LCAT (control). Glucose level was significantly reduced in K and PHU groups while the phosphorous (P) level was significantly reduced in PHU group compared to control. There were significant reductions in the total cholesterol, free cholesterol, triglycerides, total protein and albumin in K and PHU groups; whereas, significant increases in AST, GGT, non-esterified fatty acids (NEFA) and beta-hydroxybutyric acid (BHBA) in K and PHU groups were detected. Therefore, LCAT could be a predictor for metabolic disorders in Egyptian water buffaloes.  相似文献   

Response to partial replacement of yellow corn with potato processing waste as non-traditional source of energy on the productive performance of Ossimi lambs     

Hamed A. A. Omer  Soha S. Abdel-Magid  Sawsan M. Ahmed  Mamdouh I. Mohamed  I. M. Awadalla 《Tropical animal health and production》2010,42(6):1195-1202

Twenty-one male growing lambs aged 6 months with an average weight 27.6 ± 0.24 kg were used to determine the effects of partial replacing yellow corn with potato processing waste (PPW) on performance of Ossimi lambs. Animals divided into three equal groups and assigned for control and two experimental diets containing PPW which was replaced from yellow corn at 0% PPW (R₁), 25% PPW (R₂), and 50% PPW (R₃). The results showed that tested rations were almost isocaloric and isonitrogenous. Dietary treatments had no significant effect on feed intake, while water intake insignificantly decreased. Digestibility coefficients of dry matter, organic matter, and crude protein significantly (P < 0.05) improved. However, dietary treatment had no significant effect on crude fiber and nitrogen-free extract digestibilities. Values of total digestible nutrient and digestible crude protein significantly (P < 0.05) increased. Nitrogen retention was positive for all groups. Dietary treatments had no significant effect on ruminal pH but insignificantly decreased ammonia nitrogen (NH₃–N) concentrations. However, total volatile fatty acid concentration was increased. Dietary treatments increased molar proportion of volatile fatty acids. Final weight, body weight gain, and average daily gain were significantly (P < 0.05) decreased, while feed conversion ratio insignificantly decreased. Inclusion of PPW decreased total daily feeding costs of experimental rations. PPW could be used as a source of energy in lamb rations instead of corn grain. Also, PPW can be successfully fed to lamb without any adverse effect on their performance, and it can be an economical substitute for grain.  相似文献   

Effect of Needle Size and Type,Reuse of Needles,Insertion Speed,and Removal of Hair on Contamination of Joints with Tissue Debris and Hair after Arthrocentesis     

Stephen B. Adams DVM  MS  Diplomate ACVS  George E. Moore DVM  PhD  Diplomate ACVIM & ACVPM  Mohammed Elrashidy BVSc  MSc  Ahmed Mohamed BVSc  MSc  Paul W. Snyder DVM  PhD  Diplomate ACVP 《Veterinary surgery : VS》2010,39(6):667-673

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ORIGINAL ARTICLE: Echinacea purpurea and Allium sativum as immunostimulants in fish culture using Nile tilapia (Oreochromis niloticus)     

S. M. Aly  M. F. Mohamed 《Journal of animal physiology and animal nutrition》2010,94(5):e31-e39

The study was conducted to evaluate the efficiency of echnicacea (E) and garlic (G) supplemented diets as immunostimulant for tilapia (Oreochromis niloticus). Seven treatments were designed including a control (C). Fish were fed on 35% protein diet at a rate of 3% body weight per day. Echinacea (1.0 ppt) and garlic (3%) were incorporated in the feed, which was administered for periods of 1, 2 and 3 months (summer season), followed by basal diet for 4 more months (winter season). Neutrophil adherence and haematocrit values increased in both supplemented groups with prolonging period of application. The neutrophils adherence was significantly increased in all treatments except group administered echinacea for 1 month. The lymphocytic counts were significantly (p < 0.004) elevated that resulted in a significant increase in the total leucocytic count in groups administered echinacea for 1 and 2 months when compared with the control and/or other treatments. The gain in the body weight and specific growth rate was significantly increased in all supplemented groups (p < 0.004) during summer, but remained without any significant increase after winter. The survival rate was significantly high (>85%) in all the supplemented groups. The percentage of protection, after challenge infection using pathogenic Aeromonas hydrophila was the highest in groups supplemented with echinacea and garlic for 3 months after summer and winter seasons. It could be concluded that echinacea and garlic improve the gain in body weight, survival rate and resistance against challenge infection. Both compounds showed extended effects after withdrawal and improved resistance to cold stress during the winter season. However, a full commercial cost benefit analysis is necessary before recommending their application in aquaculture.  相似文献