Land contamination by toxic elements in abandoned mine areas in Italy     

Mohammad Wahsha  Mandana Nadimi-Goki  Claudio Bini 《Journal of Soils and Sediments》2016,16(4):1300-1305

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Foliar application of iron,zinc, and manganese nano-chelates improves physiological indicators and soybean yield under water deficit stress     

Mohammad Saeed Vaghar  Saeed Sayfzadeh  Hamid Reza Zakerin  Soheil Kobraee  Sayed Alireza Valadabadi 《Journal of plant nutrition》2020,43(18):2740-2756

Abstract

To investigate the effect of foliar application of nano-chelates of iron, zinc, and manganese subjected to different irrigation conditions on physiological traits, and yield of soybean (cultivar M9), a split plot experiment was conducted in a completely randomized block design with three replications in two crop years (2016–2017). The main plot included four levels of irrigation (I): full irrigation (I ₁), irrigation withhold at flowering stage (I ₂), irrigation withhold at podding stage (I ₃), and irrigation withhold during the grain filling period (I ₄). Also, the subplot included eight levels of foliar application with Fe, Zn, Mn, Fe?+?Zn, Fe?+?Mn, Zn?+?Mn, Fe?+?Zn?+?Mn nano-chelates, and distilled water (control). The results of combined analysis of variance suggested that the effect of irrigation and foliar application of nano-chelate was significant on all traits. Water deficit stress significantly reduced the grain yield. The minimum numbers of pods per plant, number of grains per plant, 100-seed weight per plant, leaf area index, leaf chlorophyll concentration, total dry weight of plant, and the grain yield were obtained by irrigation withhold at podding stage. Foliar application of combined nano-chelates increased the soybean resistance against water shortage more considerably than the separate consumption of these elements. Under drought stress in podding stage, the application of Fe?+?Zn led to the highest yield with a mean of 2613.84?kg ha^?1 where this increase was 61.1% higher than control.  相似文献   

Effects of Nitrate:Ammonium Ratios on Vegetative Growth and Mineral Element Composition in Leaves of Apple     

Mesbah Babalar  Salim Mohammad Sokri  Hosein Lesani  Mohammad Ali Asgari 《Journal of plant nutrition》2015,38(14):2247-2258

Evaluations of vegetative growth and leaf concentrations of nitrogen (N), potassium (K), calcium (Ca), magnesium (Mg), and iron (Fe) were made of apple (Malus domestica Borkh. cvs. Granny Smith, Gala, and Golab) grown with five treatments of NO₃^?:NH₄⁺ ratios in pot culture. The concentrations of NO₃^?:NH₄⁺ ratios were 2.5:0.1, 6:0.3, 6:0.5, 6:0.7, and 6: 1 meq L^?1. Regression analysis showed that growth parameters of main stems and branches were not affected by increases of NH₄⁺ in the ratios. Granny Smith, Gala, and Golab differed in some of these parameters. Concentrations of N and Fe increased as NH₄⁺ increased, whereas K and Ca decreased and Mg was not affected significantly. Generally, the treatment of 2.5:0.1 produced leaves with lower N but higher K, Ca, and Mg concentrations than the other treatments. This research showed that vegetative growth was not affected by NH₄⁺ concentration whereas elemental composition was affected.  相似文献   

Zinc Blotting Assay for Detection of Zinc‐Binding Prolamin in Barley (Hordeum vulgare) Grain     

Mohammad Nasir Uddin  Ane Langkilde‐Lauesen Nielsen  Eva Vincze 《Cereal Chemistry》2014,91(3):228-232

In plants, zinc is commonly found bound to proteins. In barley (Hordeum vulgare), major storage proteins are alcohol‐soluble prolamins known as hordeins, and some of them have the potential to bind or store zinc. ⁶⁵Zn overlay and blotting techniques have been widely used for detecting zinc‐binding protein. However, to our knowledge so far this zinc blotting assay has never been applied to detect a prolamin fraction in barley grains. A radioactive zinc (⁶⁵ZnCl₂) blotting technique was optimized to detect zinc‐binding prolamins, followed by development of an easy‐to‐follow nonradioactive colorimetric zinc blotting method with a zinc‐sensing dye, dithizone. Hordeins were extracted from mature barley grain, separated by SDS‐PAGE, blotted on a membrane, renatured, overlaid, and probed with zinc; subsequently, zinc‐binding specificity of certain proteins was detected either by autoradiography or color formation. The dithizone staining method gave similar reproducibility to the radioactive blotting. The detected zinc‐binding protein was identified as B‐hordein by Western blotting.  相似文献   

On Increasing Mustard Productivity with an Inexpensive Source of Leaf—applied Phosphorus     

Firoz  Mohammad 《Journal of Agronomy and Crop Science》1994,173(5):354-358

A simple randomized field experiment was conducted for two years to test the comparative efficacy of three sources of leaf-applied phosphorus (sodium dihydrogen orthophosphate, diammonium phosphate and monocalcium superphosphate) on the yield performance of mustard ( Brassica juncea L. Czern. and Coss.) var. Varuna. The quantity of P was 3.5 kg ha⁻¹. In addition, 20 kg N and 2 (or 6) kg S ha⁻¹ were included in the spray applied in two equal splits at 70 and 90 days as supplement to the basal application of 60 kg N, 17.5 kg P and 33.2 kg K ha⁻¹. The two-year data revealed that spray treatments were significantly efficacious in augmenting most of the yield attributing parameters as well as seed and oil yield by about 20% over the water-sprayed control. However, as the three sources of phosphorus proved equally effective, commercial grade monocalcium superphosphate could be preferred over others due to its cost effectiveness and easy availability.  相似文献   

Sero-prevalence of avian influenza among broiler-breeder flocks in Jordan     

Al-Natour MQ  Abo-Shehada MN 《Preventive veterinary medicine》2005,70(1-2):45-50

Thirty blood samples were collected randomly from each of the 38 breeder-broiler farms in Jordan. Serum samples were examined using indirect ELISA for specific antibodies to avian influenza virus. The overall true flock-level sero-prevalence of avian influenza was 71% (95% CI: 55,83). Positive flocks had 2-30 sero-positive chickens and half of flocks had >20 sero-positive birds. The number of sero-positive flocks varied in the studied localities with more sero-positives in farms located within the migratory route of migratory wild fowl. The examined broiler-breeder flocks had no clinical signs, or noticeable decrease in egg production; mortalities were within the normal range (0.1-1%). The number of positive sera/flock correlated with flock size. There were a no significant (Pearsons r=0.21, p=0.21) correlation between positive flocks and age. A non-pathogenic AI virus infects broiler-breeder farms in Jordan. Wild local and migrating birds might promote the further spread of this virus in Jordan and other countries.  相似文献   

Inhibitory effect of pyrimidine and purine nucleotides on the multiplication of Babesia gibsoni: possible cause of low parasitemia and simultaneous reticulocytosis in canine babesiosis     

Hossain MA  Yamato O  Yamasaki M  Maede Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):389-395

The present study was conducted to determine the cause of low parasitemia and simultaneous reticulocytosis in canine babesiosis. The parasitemia was significantly decreased in in vitro cultures of Babesia gibsoni by the pretreatment of host canine erythrocytes with lead acetate, which is a specific inhibitor of pyrimidine 5'-nucleotidase subclass I (P5N-I). The serum from dogs chronically infected with B. gibsoni did not decrease the activities of hexokinase, glucose-6-phosphate dehydrogenase or 6-phosphogluconate dehydrogenase in canine reticulocytes, although it was previously reported that this serum had inhibitory effects on both the maturation of reticulocytes and the canine P5N-I and purine-specific 5'-nucleotidase activities. Furthermore, the in vitro multiplication of B. gibsoni was significantly inhibited by pyrimidine nucleotides such as cytidine 5'-monophosphate (5'-CMP), which is preferentially catalyzed by P5N-I and also inhibits the morphological maturation of canine reticulocytes. Purine nucleotides such as inosine 5'-monophosphate (5'-IMP) also had an inhibitory effect on the multiplication of this parasite. These results suggest that nucleotides such as 5'-CMP and 5'-IMP might accumulate in young erythrocytes and/or serum in dogs infected with B. gibsoni as a result of the decreased activity of erythrocyte 5'-nucleotidase, and the accumulation of these nucleotides might inhibit the multiplication of this parasite and simultaneously retard the maturation of reticulocytes. The results obtained from the in vitro examinations in the present study may partially clarify the relationship between low parasitemia and simultaneous reticulocytosis in vivo in canine babesiosis.  相似文献   

A survey to identify economic opportunities for smallholder dairy farms in Bangladesh     

Shamsuddin M  Goodger WJ  Hossein MS  Azizunnesa  Bennett T  Nordlund K 《Tropical animal health and production》2006,38(2):131-140

Identified economic opportunities for planning interventions greatly increase farmers’ compliance with an extension programme. We investigated opportunities for interventions to increase dairy farmers’ income in four areas of Bangladesh, including the districts of Mymensingh, Khulna-Satkhira, Sirajgonj-Pabna and Chittagong. The data were collected from 1440 dairy farms at a one-day visit and were summarized as the difference between management targets and each herd’s calculated management indices. The average number of lactating cows, feed cost as a percentage of income from milk, milk sold as percentage of milk produced, lactating cows as a percentage of mature cows, and lactating cows as a percentage of total cattle varied from 1.5 to 3.4, from 52.5% to 92.1%, from 78.7% to 92.6%, from 81.9% to 86.7% and from 34.3% to 37.7%, respectively. The average age at first calving, calf production interval, lactation length, and milk production were 35.0–44.3 months, 14.0–17.6 months, 249–286 days and 3.5–7.2 litres, respectively, depending on the locality. The average cost for producing 100 litres of milk was 18.9–35.1 US dollars. The production cost increased when daily milk production per cow decreased (r² = 0.43–0.55). Management improvements directed towards increasing average milk production per cow per day, increasing lactation length, decreasing age to first calving, and decreasing calf production interval could expect to yield an average income increase up to a range of 676.3–1730.6 US dollars depending on the milk-producing area.  相似文献   

Characterization of a factor from bovine intestine that protects against Cryptosporidium parvum infection     

Akili D  Heidari M  Welter LM  Reinhardt TA  Harp JA 《Veterinary parasitology》2006,142(1-2):168-172

Cryptosporidium parvum is a protozoan parasite that causes intestinal infection in a variety of mammals. We have previously described a factor in adult rat or adult bovine intestinal mucosa that protects against C. parvum infection when fed to susceptible infant rats. This factor is absent in intestinal mucosa from bovine calves. In the present study we describe the further characterization of the active component of bovine intestinal mucosa. The ability to protect infant rats against C. parvum infection was found to be associated with the extrinsic membrane protein fraction of the intestinal mucosa. Extrinsic membrane preparations from adult cows, adult rats, and calves were separated by SDS-PAGE. A band with apparent molecular mass of 54 kDa was seen in preparations from adult rat and cow, but not calf. Protein was transferred to PVDF membrane and from this the band was excised and subjected to N-terminal sequence analysis using a gas-phase protein sequenator. A 15-amino acid consensus sequence was generated with homology to leucine aminopeptidase (LAP). Purified LAP was purchased from a commercial source and tested for ability to protect infant rats against C. parvum infection. Rats fed LAP from 7 to 11 days of age and challenged with C. parvum at 9 days were significantly less infected than controls upon necropsy at 15 days of age. These data suggest that a protein with N-terminal sequence homology to LAP may reduce susceptibility of infant rats to C. parvum infection.  相似文献   

Comparing apparent electrical conductivity measurements on a paddy field under flooded and drained conditions     

Mohammad Monirul Islam  Eef Meerschman  Timothy Saey  Philippe De Smedt  Ellen Van De Vijver  Marc Van Meirvenne 《Precision Agriculture》2012,13(3):384-392

Every growing season, paddy fields are kept both flooded and drained for a significant period of time. As a consequence, these soils develop distinct physico-chemical characteristics. For practical reasons, these soils are mostly sampled under dry conditions, but the question arises how representative the results are for the wet growing conditions. Therefore, the apparent electrical conductivity (EC_a) of a 1.4 ha alluvial paddy field located in the Brahmaputra floodplain of Bangladesh was measured in both dry and wet conditions by a sensing system using the electromagnetic induction sensor EM38, which does not require physical contact with the soil, and compared both surveys. Due to the smooth water surface under wet conditions which ensured increased stability of the sensing platform, the results of the survey showed considerably reduced micro-scale variability of EC_a. Furthermore, the wet survey results more reliably furnished soil-related information mainly due to the absence of soil moisture dynamics. The differences between EC_a under wet and dry conditions were attributed to differences in soil texture, mainly the sand content variation having considerable effect on soil moisture differences when flooded following drainage. Accordingly, the largest differences between EC_a under wet and dry conditions were found in those parts of the field with a large sand content. Hence, the conclusion was that an EC_a survey on flooded fields has an added value to precision soil management.  相似文献