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991.
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Different protein fractions precipitated with ammonium sulphate from extracts of healthy andFusarium-infected tomato plants showed a linear relationship between polyphenoloxidase activity and ability to form indoleacetic acid (IAA) from tryptophan in the presence of chlorogenic acid. Dialysis slightly reduced polyphenoloxidase activity of as well as IAA formation by crude and purified extracts.Polyphenoloxidase as well as IAA production were partially inhibited by DIECA, EDTA, cystein and ascorbic acid. The inhibition was shown to occur in crude and purified extracts and stem slices of both healthy and diseased plants, but was consistently higher in material from diseased plants.The reported results may be considered to further confirm the strict interdependence between polyphenoloxidase activity and ability to form IAA in tomato plants. The different sensitivity to some inhibitors shown by material from healthy and from diseased plants is emphasized.  相似文献   
993.
Experiments on the effect of various compounds with plant growth regulating activity led to the hypothesis that conditions inhibitory to indoleacetic acid (IAA) action or leading to a decrease in the IAA level in cucumber seedlings would be unfavourable for the development of cucumber scab, caused byCladosporium cucumerinum. Susceptibility decreased as the result of treatment with growth retardants, which cause an increase in IAA-oxidase activity in the plants, whereas application of indoleacetic acid or compounds expected to decrease the rate of oxidative breakdown of IAA in the seedlings resulted in an increase of susceptibility. Growing the plants under various periods of illumination also influenced both the susceptibility of the plants and the IAA-oxidase activity in the hypocotyl tissue.To obtain further information about the relation between the increase in resistance and the increase in the rate of IAA oxidation, the effect of one compound, viz. L-threo-β-phenylserine (abbr. phenylserine) was studied in more detail. The rate of oxidative breakdown of IAA was increased in extracts of hypocotyls and cotyledons of plants treated with phenylserine as compared with extracts of tissue of untreated plants. Extracts of phenylserine-treated plants were found to contain a higher cofactor and/or a lower inhibitor content than those of control plants. The substances involved were not identified, but this result may indicate a shift in the concentrations of various phenols in the tissue.The question must remain open whether a shift in the concentration of phenols some-how effected by phenylserine treatment determines both degree of susceptibility and rate of IAA oxidation independently, or whether the rate of IAA breakdown and the resulting change in IAA content in the plants is directly related to the degree of susceptibility.  相似文献   
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Witches' broom phenomena (witches' broom growth + antholysis) observed inTropaeolum majus and in clovers in the Netherlands and in several wild and cultivated plants in Italy were demonstrated to be due to virus infection. The virus(es) could be transmitted by grafting, through dodder and by means of leaf-hoppers (Euscelis spp. primarily). The virus isolates found in both countries did not differ appreciably in the symptoms they caused. Many of the deviations concerned have been known for a long time as teratological phenomena. In the extensive literature considerable confusion exists concerning the identity of several witches' broom virus diseases. They constitute a good example of the problem of virus variability. A continuous splitting of witches' broom viruses on the basis of slight differences in symptom expression or of vector specificity does not seem justified. Since no intrinsic properties of the virus(es) concerned are known, the only conclusion that can be drawn from the information available is that the Dutch and the Italian isolates show a great similarity to aster yellows virus as well as to tomato big bud, stolbur and other witches' broom viruses.  相似文献   
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