Characterization of “Mais delle Fiorine” (Zea mays L.) and nutritional,morphometric and genetic comparison with other maize landraces of Lombardy region (Northern Italy)     

Giupponi  Luca  Leoni  Valeria  Colombo  Federico  Cassani  Elena  Hejna  Monika  Rossi  Luciana  Pilu  Roberto 《Genetic Resources and Crop Evolution》2021,68(5):2075-2091

Genetic Resources and Crop Evolution - The loss of agrobiodiversity is a topic of global impact. On a local scale, Lombardy, in the Alpine macro-Region, has lost more than 78% of its plant...  相似文献   

Phenotypic evaluation and characterization of a collection of Malus species     

Monika Höfer  Mohamed Ali Mohamed Saad Eldin Ali  Jörg Sellmann  Andreas Peil 《Genetic Resources and Crop Evolution》2014,61(5):943-964

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Analysis of glycated and ascorbylated proteins by gas chromatography-mass spectrometry     

Hasenkopf K  Rönner B  Hiller H  Pischetsrieder M 《Journal of agricultural and food chemistry》2002,50(20):5697-5703

Proteins or poly-L-lysine which were incubated in the presence of ascorbic acid, dehydroascorbic acid (ascorbylation), or various sugars (glycation) were analyzed by gas chromatography-mass spectrometry (GC-MS). To also detect more labile reaction products, the Maillard modified proteins or poly-L-lysine were enzymatically hydrolyzed and reacted with N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide to form the N(O)-tert-butyldimethylsilyl (tBDMS) derivatives prior to GC analysis. Under these conditions, the known Maillard products N (epsilon)-(carboxymethyl)lysine (1), oxalic acid mono-N (epsilon)-lysinylamide (2), and N (epsilon)-(carboxyethyl)lysine (3) could be simultaneously detected and quantified in glycated and ascorbylated proteins. Additionally, N (epsilon)-(1-carboxy-3-hydroxypropyl)-L-lysine (4) was identified for the first time as a Maillard product of proteins. Under the conditions applied here, 4 was found only in ascorbylated proteins or poly-L-lysine, but not in glycated proteins. Maillard-modified poly-L-lysine was further subjected to high-performance liquid chromatography (HPLC) analysis after enzymatic hydrolysis and formation of the phenyl isothiocyanate derivatized amino acids. Using this method, N (epsilon)-formyl-L-lysine (5), which cannot be distinguished from 2 by GC-MS analysis, was identified for the first time as a glycation product. Compound 5 is mainly formed from ribose, lactose, and fructose. The indicated Maillard products were quantified in beta-lactoglobulin (GC-MS) or poly-L-lysine (HPLC) which were glycated or ascorbylated using different precursors.  相似文献   

A catena in northeast Brandenburg (Bölkendorf): Soil biota,root growth and soil structure     

Monika Joschko  Helmut Rogasik  Margitta Dannowski  Wilfried Hierold  Claus Heisler 《Archives of Agronomy and Soil Science》2013,59(5):385-396

Typical for the morainal landscape of NE‐Germany are catenas with pronounced gradients of abiotic factors, such as soil texture and organic matter content. For adequate management of these regions, their ecology must be better understood. In this context, the biological interactions in soil play an important role. The present study is based on the hypothesis that the gradients of abiotic factors across the catena are reflected in soil biota, i.e. earthworms and microarthropods, and root growth.

Sampling was carried out at selected positions across the catenary sequence of Boelkendorf, Brandenburg, for earthworms, Collembola and roots in April 1994. The soil physical analysis comprised measurements of soil bulk density and penetration resistance.

A positive correlation between earthworm abundance, numbers of Collembola and root growth at different catena positions was found. The top position especially, a calcaric regosol with low organic matter content, was markedly different with respect to soil biological and physical characteristics. High bulk densities and high soil resistance in the subsoil coincided with low earthworm densities, low microarthropod numbers and impeded rootability of the soil.  相似文献   

Assessment of phenotypic variation of Malus orientalis in the North Caucasus region     

Monika Höfer  Henryk Flachowsky  Magda-Viola Hanke  Valentin Semënov  Anna Šlâvas  Irina Bandurko  Artëm Sorokin  Sergej Alexanian 《Genetic Resources and Crop Evolution》2013,60(4):1463-1477

Malus orientalis Uglitzk. is the predominant Malus species of the Caucasian forests distributed in the north of Anatolia, Armenia, Russia as well as in Iran. It is considered as one of the probable minor ancestors of domestic apples. Although M. orientalis has a lower diversity of fruit quality, other valuable traits such as later blooming, adaptation to a wider array of habitats, and capacity for longer storage of the apples should be taken into account for improving the genetic makeup of the domestic apple. A joint expedition of scientists of the Julius Kühn-Institute from Germany and the Nikolaj I. Vavilov Research Institute of Plant Industry from Russia was performed into the North Caucasus region during August/September 2011. Altogether 101 M. orientalis accessions were collected from eight sites at the North Caucasus. Twenty-six traits such as size, color, shape, flavor and firmness of fruit and tree habit were used for phenotypic evaluation of the accessions. A high phenotypic diversity within the collected material of M. orientalis was indicated. Accessions characterized by suitable fruit traits like bigger size, larger cover color, less bitterness and better firmness as well as more sweetness and better flavor were found. However, small-sized flavorless fruits were also detected. Tree habit varied widely from upright to weeping. Subsequently, a comprehensive phenotypic and genetic evaluation of M. orientalis increases the knowledge of diversity, may provide new resources of agronomically important traits for breeding purposes, and gives support to determine accessions for a core collection.  相似文献   

Methods for the very early selection of Pinus radiata D. Don. for solid wood products     

Shakti S. Chauhan  Monika Sharma  Jimmy Thomas  Luis A. Apiolaza  David A. Collings  John C. F. Walker 《Annals of Forest Science》2013,70(4):439-449

? Introduction

There has been an increasing interest in very early selection of radiata pine to reduce the breeding cycle for solid wood products. For such selection, new approaches are required to assess wood quality in wood from very young stems.

? Methods

Nursery seedlings of clones of radiata pine were grown in leant condition using two leaning strategies for 18–20 months. Opposite wood and compression wood were isolated from the leaning stems and tested for dynamic modulus of elasticity, density, longitudinal shrinkage, volumetric shrinkage and compression wood area using new methods evolved for testing small size samples quickly and reliably. The methods were tested for their efficiency in differentiating clones by their wood properties.

? Results

Leaning of stems provided distinct opposite and compression wood for testing. Automated image analysis method used for compression wood area assessment was found to be a quick and effective method for processing large number of samples from young stems. Compression wood was characterised by high basic density, high longitudinal shrinkage and low volumetric shrinkage than that of opposite wood. Acoustic velocity in opposite wood had a strong negative association with longitudinal shrinkage. The study signifies the importance of preventing mixing of opposite wood with compression wood while assessing wood quality in young stems thus making leaning a critical strategy. The comparison of wood properties of opposite wood revealed significant differences between clones. Opposite wood of the clone with the lowest dynamic modulus of elasticity exhibited the highest longitudinal shrinkage.

? Conclusion

Significant differences in measurable wood properties between clones suggest the prospects of early selection for solid wood products.  相似文献   

Large-scale genetic structure and drought-induced effects on European Scots pine (Pinus sylvestris L.) seedlings     

Steffen Taeger  Barbara Fussi  Monika Konnert  Annette Menzel 《European Journal of Forest Research》2013,132(3):481-496

Seedlings of ten provenances of Scots pine (Pinus sylvestris L.) from the southwestern to the central part of the species distribution range were investigated in a greenhouse experiment under dry conditions compared to a well-watered control. We conducted an isozyme study and recorded phenology as well as growth traits during the first year of growth. Genetic variability and genetic diversity increased from the westernmost provenances to those central to the distribution. Provenances from the Apennines and Spain revealed the strongest genetic differentiation compared to all others, whereas populations from Germany, Poland and Bulgaria were found to belong to the same gene pool. Seedling development and bud set were faster in northern populations. Shoot length was highest for Polish and German provenances within both regimes, populations from France and Spain had the longest roots. Reduced soil moisture slowed later development stages and delayed bud set for all provenances by about 5 days. Shoot growth decreased considerably under the dry regime for all provenances. German provenances were the most sensitive to drought, whereas Spanish provenances showed adaptation to drought conditions indicated by the lowest reduction in shoot growth relative to optimum conditions. The results of this pilot study show that under more pronounced drought conditions with repeated drought events, the so far established superiority of northeastern provenances compared to southwestern ones could diminish in the future.  相似文献   

Serum biochemical reference intervals for the Polish Konik horse (Equus cabalus gmelini Ant.)     

Artur Niedźwiedź  Zbigniew Jaworski  Henryk Filipowski  Marcin Zawadzki  Marcin Wrzosek  Monika Służewska‐Niedźwiedź  Józef Nicpoń 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2013,42(1):66-69

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Ileal absorption of L-carnitine from diets used in reproductive polar fox (Alopex lagopus L.) nutrition     

Roman Szymeczko  Katarzyna Burlikowska  Christine Iben  Anna Piotrowska  Monika Bogusławska-Tryk 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(3):142-147

Abstract

Five mature ileorectal anastomosed blue foxes (6.18±0.15 kg) were used in digestibility experiments to evaluate the L-carnitine apparent ileal absorption from diets used in reproductive polar fox nutrition over the year-long farm-feeding period on two domestic farms (A and B) differing in reproduction results. The concentration of L-carnitine was higher in diets from farm B (136.1–241.7 mg kg^?1 DM) than in the diets from farm A (88.5–135.2 mg kg^?1 DM). The coefficients of ileal apparent absorption of L-carnitine ranged from 60.23 to 75.57% for diets A (farm A) composed mainly of fish and poultry offals. The coefficients of ileal apparent digestibility of L-carnitine were higher for diets B (farm B) (83.75–94.78%; P<0.05) based on beef offals. These data indicate high concentration and efficient ileal apparent absorption of L-carnitine from diets used in reproductive polar fox nutrition on both farms examined.  相似文献   

Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines     

Petra Ondrackova  Lenka Leva  Zdenka Kucerova  Monika Vicenova  Marketa Mensikova  Martin Faldyna 《Veterinary research》2013,44(1):98

Monocytes play an essential role in the defense against bacterial pathogens. Bone marrow (BM) and peripheral blood (PB) monocytes in pigs consist of the main “steady-state” subpopulations: CD14^hi/CD163^-/SLA-DR^- and CD14^low/CD163⁺/SLA-DR⁺. During inflammation, the subpopulation of “inflammatory” monocytes expressing very high levels of CD163, but lacking the SLA-DR molecule (being CD14^low/CD163⁺/SLA-DR^-) appears in the BM and PB and replaces the CD14^low/CD163⁺/SLA-DR⁺ subpopulation. However, current knowledge of monocyte migration into inflamed tissues in pigs is limited. The aim of the present study was to evaluate the distribution of “inflammatory” CD14^low/CD163⁺/SLA-DR^- monocytes during experimental inflammation induced by Actinobacillus pleuropneumoniae (APP) and a possible role for chemokines in attracting “inflammatory” CD14^low/CD163⁺/SLA-DR^- monocytes into the tissues. Monocyte subpopulations were detected by flow cytometry. Chemokines and chemokine receptors were detected by RT-qPCR. The “steady-state” monocytes were found in the BM, PB, spleen and lungs of control pigs. After APP-infection, “inflammatory” monocytes replaced the “steady-state” subpopulation in BM, PB, spleen and moreover, they appeared in an unaffected area, demarcation zone and necrotic area of the lungs and in tracheobronchial lymph nodes. They did not appear in mesenteric lymph nodes. Levels of mRNA for various chemokines with their appropriate receptors were found to be elevated in BM (CCL3-CCR1/CCR5, CCL8-CCR2/CCR5, CCL19-CCR7), necrotic area of the lungs (CCL3-CCR1, CCL5-CCR1/CCR3, CCL11-CCR3, CCL22/CCR4) and tracheobronchial lymph nodes (CCL3-CCR1) and therefore they could play a role in attracting monocytes into inflamed tissues. In conclusion, “inflammatory” monocytes appear in different lymphoid tissues and the lungs after APP infection in pigs. Various chemokines could drive this process.  相似文献