首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   447篇
  免费   29篇
林业   31篇
农学   22篇
  130篇
综合类   19篇
农作物   24篇
水产渔业   11篇
畜牧兽医   192篇
园艺   11篇
植物保护   36篇
  2024年   1篇
  2023年   2篇
  2022年   2篇
  2021年   18篇
  2020年   22篇
  2019年   15篇
  2018年   14篇
  2017年   22篇
  2016年   23篇
  2015年   24篇
  2014年   21篇
  2013年   41篇
  2012年   43篇
  2011年   28篇
  2010年   25篇
  2009年   15篇
  2008年   31篇
  2007年   24篇
  2006年   21篇
  2005年   17篇
  2004年   12篇
  2003年   10篇
  2002年   13篇
  2001年   4篇
  2000年   5篇
  1999年   3篇
  1998年   1篇
  1997年   1篇
  1995年   4篇
  1994年   2篇
  1993年   2篇
  1992年   2篇
  1991年   3篇
  1988年   1篇
  1986年   1篇
  1983年   1篇
  1982年   1篇
  1980年   1篇
排序方式: 共有476条查询结果,搜索用时 879 毫秒
421.
Histological sections of femoral diaphysis from adult sheep were investigated in this study. The qualitative characteristics in microstructure of the compact bone were examined in anterior, posterior, medial and lateral views; the quantitative ones were assessed using the software Scion Image. Areas, perimeters, minimum and maximum diameters of the Haversian canals, the Haversian systems and the primary osteons' vascular canals were measured. Our results indicate that ovine diaphysis of the femur is occupied mainly with primary vascular plexiform (laminar, fibrolamellar) tissue. In addition, irregular and dense Haversian bone tissues were observed. All measured variables disposed lower values in comparison with other artiodactyls (cattle and pig).  相似文献   
422.
423.
 We have estimated the production of water-soluble B vitamins by plant growth-promoting rhizobacterium Pseudomonas fluorescens strain 267 in a minimal medium with different C sources and at different pH values. In the minimal medium, strain 267 produced large amounts of niacin (0.92 μg ml–1) and pantothenic acid (0.75 μg ml–1), but also other vitamins such as biotin, thiamine, cobalamine and pyridoxine. The production of B vitamins was dependent on the C source and pH of the growth medium. By random Tn5 mutagenesis, thiamine and niacin auxotrophs were isolated from P. fluorescens strain 267 and mutants were used to evaluate the vitamin production on colonization of clover roots under controlled conditions. Red clover root colonization decreased by about 1 order of magnitude in the case of the niacin auxotroph. The vitamin auxotrophs of P. fluorescens in a mixed inoculation of clover with R. leguminosarum bv. trifolii strain 24.1 showed no plant growth-promotion activity. Received: 23 May 2000  相似文献   
424.
Pepper yellow mosaic virus (PepYMV) is the most important potyvirus infecting sweet pepper in Brazil. In this study, twenty isolates of PepYMV were obtained from commercial sweet pepper crops. To confirm virus identity, the coat protein gene was completely sequenced for eleven of these isolates, and partially sequenced for the other nine isolates. The amino acid identities obtained were above 93% when compared with the sequence of a characterized PepYMV isolate (AF348610). Extracts of Nicotiana tabacum cv. TNN plants infected with the different isolates were used to inoculate the differential series of Capsicum spp cultivars containing the genes pvr2 1 , pvr2 2 , pvr2 3 , pvr2 4 , and Pvr4. Using the same criteria established for Potato virus Y (PVY), fourteen isolates of PepYMV could be classified as known pathotypes described for PVY, that is: 1.2 (2 isolates), 1.3 (6) and 1.2.3 (6). The remaining six isolates, 1.3 (2) and 1.2.3 (4) could not be classified into the typical pathotypes of PVY because they were also virulent on Serrano Criollo de Morellos—334 (C.M 334) which carries the pvr2 3 and Pvr4 genes. To classify the PepYMV into pathotypes and counter the biological diversity found in this species we propose the utilization of 2x for the ability to overcome the correspondent allele of the pvr2 locus and 4 for the capacity to break down the Pvr4 gene. Using this criterion we could classify the PepYMV into five pathotypes: 21.22; 21.23; 21.22.23; 21.23. 4 and 21.22.23. 4.  相似文献   
425.
426.
427.
428.
429.
The objective of the present study was to evaluate the effect of the bread supplemented with whole amaranth flour (0, 20 and 40%) on iron bioavailability using Caco-2 cells model. The phytate and lower myo-inositol phosphates content in in vitro bread digests were measured by high pressure liquid chromatography. The breads made with amaranth showed significant increase of soluble phytates levels (up to 1.20 μmol/g in dry matter for the 40% of substitution) in comparison with controls, which have not detectable values. A negative correlation among phytate and Fe availability was found when increased levels of amaranth. Ferritin concentration was found 2.7- and 2.0-fold higher (P < 0.05) in cultures exposed to 20% and 40% of amaranth formulated bread samples, respectively, compared to control bread. The soluble phytate/Fe molar ratio explained the whole amaranth flour-mediated inhibitory effect associated to the limitation of available Fe; however, the use up to 20% of amaranth in bread formulation appears as a promising strategy to improve the nutritional value of bread, as indicated by the ferritin concentrations quantified in cell cultures. Higher proportion of amaranth flour increased Fe concentration although there was not detected any increase in Fe uptake.  相似文献   
430.
Kynurenic acid (KYNA) is a metabolite of tryptophan which is formed along the kynurenine pathway. KYNA may possess neuroprotective, anti-inflammatory, antioxidant and antiproliferative properties. This study measured the concentration of KYNA in various varieties of potatoes and products made from potatoes. KYNA content was determined by means of the high-performance liquid chromatography with fluorescence detection. KYNA was found in all 16 studied varieties of potato tubers in amounts varying from 0.239 to 3.240 μg/g dry weight. The content of KYNA in potato tubers declined during long-term storage. The content of KYNA in French fries varied from 0.100 to 0.646 μg/g dry weight. KYNA content in potato crisps was 0.478 and 0.576 μg/g dry weight. Hence, all in all, we concluded that the amount of KYNA potentially delivered to the human body in potatoes and various foods produced from potatoes is high and might be compared to the amount of KYNA present in a maximum daily dose of popular herbs and herbal medicines.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号