全文获取类型
收费全文 | 398篇 |
免费 | 27篇 |
专业分类
林业 | 11篇 |
农学 | 11篇 |
基础科学 | 2篇 |
57篇 | |
综合类 | 23篇 |
农作物 | 109篇 |
水产渔业 | 45篇 |
畜牧兽医 | 156篇 |
园艺 | 2篇 |
植物保护 | 9篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 7篇 |
2020年 | 10篇 |
2019年 | 15篇 |
2018年 | 25篇 |
2017年 | 15篇 |
2016年 | 14篇 |
2015年 | 14篇 |
2014年 | 27篇 |
2013年 | 31篇 |
2012年 | 23篇 |
2011年 | 37篇 |
2010年 | 16篇 |
2009年 | 19篇 |
2008年 | 22篇 |
2007年 | 25篇 |
2006年 | 22篇 |
2005年 | 14篇 |
2004年 | 19篇 |
2003年 | 25篇 |
2002年 | 10篇 |
2001年 | 14篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1997年 | 1篇 |
1989年 | 1篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有425条查询结果,搜索用时 31 毫秒
91.
Min‐Ok Ryu Byung‐Gak Kim Ul‐Soo Choi Kwan‐Hyuck Baek Young‐Ki Song Qiang Li Kyoung‐Won Seo Sandra Ryeom Hwa‐Young Youn Dong‐Ha Bhang 《Veterinary and comparative oncology》2019,17(1):99-106
Protein kinase A, a cyclic adenosine monophosphate (AMP)‐dependent enzyme, normally exists within mammalian cells; however, in cancer cells, it can leak out and be found in the serum. Extracellular cyclic AMP‐dependent protein kinase A (ECPKA) has been determined to increase in the serum of cancer‐bearing dogs. However, there have been no reports in the veterinary literature on serum ECPKA autoantibody (ECPKA‐Ab) expression in dogs with cancer. The aim of this study was to evaluate ECPKA‐Ab and C‐reactive protein (CRP) as serum biomarkers for cancer in dogs. ECPKA‐Ab and CRP levels were detected by an enzyme‐linked immunosorbent assay in serum samples from dogs with malignant tumours (n = 167), benign tumours (n = 42), or non‐tumour disease (n = 155) and from healthy control dogs (n = 123). ECPKA‐Ab and CRP levels were significantly higher in the dogs with malignant tumours than in those with benign tumours or non‐tumour diseases, as well as in the healthy controls (P < 0.001, Kruskal‐Wallis test). There was a significant positive correlation between the neoplastic index, which was developed using ECPKA‐Ab and CRP levels, and the presence of cancer in dogs (P < 0.001); the area under the receiver‐operating characteristic curve was estimated to be >0.85 (P < 0.001). In conclusion, ECPKA‐Ab is a potential serum biomarker for a broad spectrum of cancers. Combined measurement of CRP and ECPKA‐Ab levels in serum improves the sensitivity and accuracy of a diagnosis of cancer in dogs. 相似文献
92.
93.
Sung‐Eun Lee Byoung‐Ho Lee Won‐Sik Choi Byeoung‐Soo Park Jeong‐Gyu Kim Bruce C Campbell 《Pest management science》2001,57(6):548-553
The fumigant toxicity of various volatile constituents of essential oils extracted from sixteen Korean spices and medicinal plants towards the rice weevil, Sitophilus oryzae L (Coleoptera: Curculionidae), was determined. The most potent toxicity was found in the essential oil from Mentha arvensis L var piperascens (LC50 = 45.5 µl litre?1 air). GC–MS analysis of essential oil from M arvensis showed it to be rich in menthol (63.2%), menthone (13.1%) and limonene (1.5%), followed in abundance by β‐pinene (0.7%), α‐pinene (0.6%) and linalool (0.2%). Treatment of S oryzae with each of these terpenes showed menthone to be most active (LC50 = 12.7 µl litre?1 air) followed by linalool (LC50 = 39.2 µl litre?1 air) and α‐pinene (LC50 = 54.9 µl litre?1 air). Studies on inhibition of acetylcholinesterase activity of S oryzae showed menthone to have a nine‐fold lower inhibitory effect than menthol, despite menthone being 8.1‐fold more toxic than menthol to the rice weevil. Different modes of toxicity of these monoterpenes towards S oryzae are discussed. © 2001 Society of Chemical Industry 相似文献
94.
95.
Pharmacokinetic profiles of metamizole (dipyrone) active metabolites in goats and its residues in milk
下载免费PDF全文
![点击此处可从《Journal of veterinary pharmacology and therapeutics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Tae Won Kim Andrejs Sitovs Amnart Poapolathep Helen Owen Andrzej Lisowski Zulkyya Abilova Mario Giorgi 《Journal of veterinary pharmacology and therapeutics》2018,41(5):699-705
Metamizole (dipyrone, MET) is a nonopioid analgesic drug commonly used in human and veterinary medicine. The aim of this study was to assess two major active metabolites of MET, 4‐methylaminoantipyrin (MAA) and 4‐aminoantipyrin (AA), in goat plasma after intravenous (IV) and intramuscular (IM) administration. In addition, metabolite concentration in milk was monitored after IM injection. Six healthy female goats received MET at a dose of 25 mg/kg by IV and IM routes in a crossover design study. The blood and milk samples were analyzed using HPLC coupled with ultraviolet detector and the plasma vs concentration curves analyzed by a noncompartmental model. In the goat, the MET rapidly converted into MAA and the mean maximum concentration was 183.97 μg/ml (at 0.08 hr) and 51.94 μg/ml (at 0.70 hr) after IV and IM administration, respectively. The area under the curve and mean residual time values were higher in the IM than the IV administered goats. The average concentration of AA was lower than MAA in both groups. Over 1 μg/ml of MAA was found in the milk (at 48 hr) after MET IM administration. In conclusion, IM is considered to be a better administration route in terms of its complete absorption with long persistence in the plasma. However, this therapeutic option should be considered in light of the likelihood of there being milk residue. 相似文献
96.
97.
98.
Transient ischaemia affects plasma membrane glutamate transporter, not vesicular glutamate transporter, expressions in the gerbil hippocampus 总被引:3,自引:0,他引:3
Kim DS Kwak SE Kim JE Jung JY Won MH Choi SY Kwon OS Kang TC 《Anatomia, histologia, embryologia》2006,35(4):265-270
In the present study, we investigated expressions of vesicular glutamate transporter (VGLUT) and of the plasma membrane glutamate transporters [glutamate transporter 1 (GLT-1), glutamate/aspartate transporter (GLAST) and excitatory amino acid carrier 1 (EAAC-1)] in the gerbil hippocampus following transient ischaemia. The expressional levels and distribution patterns of VGLUT immunoreactivities were unaltered until 3 days after ischaemic-insults. However, VGLUT-2 immunoreactivity in the CA1 region was reduced at 4 days after ischaemia due to delayed neuronal death. In addition, both GLT-1 and GLAST immunoreactivities in the CA1 region were enhanced at 30 min - 12 h after ischaemia-reperfusion and their expression began to reduce at 24 h after ischaemia-reperfusion. In contrast, EAAC-1 immunoreactivity was transiently reduced in the CA1 region at 30 min after ischaemia, re-enhanced at 3-12 h after ischaemia, and re-reduced at 24 h after ischaemia. These findings suggest that malfunctions of plasma membrane glutamate transporters, not of VGLUT, may play an important role in the elevation of extracellular glutamate concentration following ischaemic insults. 相似文献
99.
Yoo KY Hwang IK Lee JC Cho JH Kim SM Jung JY Kang TC Won MH 《Anatomia, histologia, embryologia》2006,35(2):93-96
Information on the localization and the roles of glutamate in the nervous system is becoming valuable because the axon terminals of the olfactory sensory neurons and the synapses of the mitral and tufted output cells appear to be glutamatergic. In this study, we have analysed the distribution of glutamate immunoreactivity in the main olfactory bulb (MOB) of the Mongolian gerbil using an antiserum directed against glutamate. Glutamate immunoreactivity in the MOB was present in the olfactory nerve layer (Onl), glomerular layer (GL), external plexiform layer (EPL) and mitral cell layer (ML), but not in the granule cell layer (GCL). Glutamate immunoreactivity detected in the Onl was thought to be terminal ramifications of glomeruli. Some neurons in the periglomerular region showed glutamate immunoreactivity. In the EPL, glutamate immunoreactivity was found in some neuronal somata (tufted cells) and processes. In addition, mitral cells in the ML were labelled by the glutamate antibody. The pattern of glutamate immunoreactivity in the mitral cells was similar to that in the tufted cells. In brief, glutamate in the gerbil MOB is the neurotransmitter used by primary afferents and output neurons. 相似文献
100.
Jeong YG Chung SH Kim CT Kim KH Han SY Hyun BH Lee NS Sawada K Won MH Fukui Y 《Anatomia, histologia, embryologia》2006,35(2):111-115
The ataxic pogo mouse (pogo/pogo) is a novel neurological mutant, which was derived as an inbred strain (KJR/MsKist) from a Korean wild mouse. The pathological manifestations include a difficulty in maintaining a normal posture, the failure of inter-limb coordination and an inability to walk straight. In this study, we examined the distribution of corticotropin-releasing factor (CRF) immunoreactive cerebellar climbing fibres and their projections to tyrosine hydroxylase (TH) immunoreactive Purkinje cells in the cerebellum of the pogo mutant mouse using immunohistochemistry. In the pogo/pogo mouse, a subset of climbing fibres was stained more intensely for CRF than in the control. Moreover, ataxic pogo mouse, neurons of the inferior olivary nucleus projecting climbing fibres were also more intensely stained for CRF than in the control. In the pogo/pogo mouse, TH immunoreactivity was located in the Purkinje cells, whereas no TH expression was found in the control. Double immunostaining for CRF and TH in the pogo/pogo cerebellum revealed that the distribution of TH-immunoreactive Purkinje cells corresponded to terminal fields of CRF-immunoreactive climbing fibres but not to the CRF-immunoreactive mossy fibres. Therefore, we suggest that an increase of CRF level may alter the function of targeted Purkinje cells and that it is related to the ataxic phenotype in the pogo mutant mouse. 相似文献