Expression of CD44 adhesion molecule in rat testis with ischemia/reperfusion injury     

Moon C  Jeong CW  Kim H  Ahn M  Kim S  Shin T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(7):761-764

The expression pattern of CD44 was studied in the rat testis following ischemia/reperfusion (I/R) injury to elucidate the possible role of the CD44 adhesion molecule in acute experimental testicular torsion. Western blot analysis showed that CD44 expression began to increase significantly 24 hr after reperfusion, compared with the normal control; the increased expression persisted until 96 hr after I/R. Immunohistochemistry showed that, in the normal testis, CD44 was constitutively expressed mainly in ED2-positive resident macrophages in the interstitial space. After I/R, the majority of inflammatory cells in the interstitial space surrounding the damaged tubules were ED1-positive macrophages that were CD44-positive. These findings suggest that the significant increase in CD44 expression that occurs during the delayed phase after reperfusion originates from infiltrating macrophages possibly in anticipation of the migration and adhesion of additional macrophages into the affected testis.  相似文献   

A synthetic fraction of feline facial pheromones calms but does not reduce struggling in cats before venous catheterization     

Kronen PW  Ludders JW  Erb HN  Moon PF  Gleed RD  Koski S 《Veterinary anaesthesia and analgesia》2006,33(4):258-265

OBJECTIVE: To evaluate whether a synthetic analogue of feline facial pheromone (FFP) calms cats before, and reduces struggling during intravenous catheterization. DESIGN: Block-randomized, 'blinded' clinical trial. ANIMALS: Seventy-seven healthy cats presented for elective surgery. PROCEDURE: Cats given glycopyrrolate and oxymorphone were assigned to one of four treatments: acepromazine and exposure to FFP (aceFFP); acepromazine and exposure to a placebo (acePlac); exposure to FFP only (FFP) and exposure to placebo only (Plac). The behaviour of cats was recorded on videotape for evaluation by assessors unaware of treatment group. Cats' veins were then catheterized by veterinary students unaware of the study protocol. Based on each cat's response to catheterization, the student independently decided whether intramuscular ketamine was required. RESULTS: Cats in the aceFFP group appeared to be calmer than acePlac cats on the basis of head position and their location in the cage (suggesting benefit from FFP among cats receiving acepromazine) but appeared to be less sedated. Cats in the aceFFP group also appeared to be calmer than FFP cats on the basis of head position and location in the cage. Feline facial pheromone cats were also calmer than Plac cats when compared using body and leg position. Exposure to FFP did not significantly reduce struggling at catheterization, nevertheless, the students were able to catheterize the veins in all cats. CONCLUSION AND CLINICAL RELEVANCE: There were no detrimental behavioural effects associated with either FFP or acepromazine. The FFP had additional calming effects in cats given acepromazine and, to a lesser degree, helped to calm cats that were not given acepromazine. Feline facial pheromone helps to calm cats in unfamiliar surroundings.  相似文献   

Tracing soybean domestication history: From nucleotide to genome     

Moon Young Kim  Kyujung Van  Yang Jae Kang  Kil Hyun Kim  Suk-Ha Lee 《Breeding Science》2012,61(5):445-452

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Recombinant Iss as a potential vaccine for avian colibacillosis     

Lynne AM  Kariyawasam S  Wannemuehler Y  Johnson TJ  Johnson SJ  Sinha AS  Lynne DK  Moon HW  Jordan DM  Logue CM  Foley SL  Nolan LK 《Avian diseases》2012,56(1):192-199

Avian pathogenic Escherichia coli (APEC) cause colibacillosis, a disease which is responsible for significant losses in poultry. Control of colibacillosis is problematic due to the restricted availability of relevant antimicrobial agents and to the frequent failure of vaccines to protect against the diverse range of APEC serogroups causing disease in birds. Previously, we reported that the increased serum survival gene (iss) is strongly associated with APEC strains, but not with fecal commensal E. coli in birds, making iss and the outer membrane protein it encodes (Iss) candidate targets for colibacillosis control procedures. Preliminary studies in birds showed that their immunization with Iss fusion proteins protected against challenge with two of the more-commonly occurring APEC serogroups (O2 and O78). Here, the potential of an Iss-based vaccine was further examined by assessing its effectiveness against an additional and widely occurring APEC serogroup (O1) and its ability to evoke both a serum and mucosal antibody response in immunized birds. In addition, tissues of selected birds were subjected to histopathologic examination in an effort to better characterize the protective response afforded by immunization with this vaccine. Iss fusion proteins were administered intramuscularly to four groups of 2-wk-old broiler chickens. At 2 wk postimmunization, chickens were challenged with APEC strains of the O1, O2, or O78 serogroups. One week after challenge, chickens were euthanatized, necropsied, any lesions consistent with colibacillosis were scored, and tissues from these birds were taken aseptically. Sera were collected pre-immunization, postimmunization, and post-challenge, and antibody titers to Iss were determined by enzyme-linked immunosorbent assay (ELISA). Also, air sac washings were collected to determine the mucosal antibody response to Iss by ELISA. During the observation period following challenge, 3/12 nonimmunized chickens, 1/12 chickens immunized with 10 microg of GST-Iss, and 1/12 chickens immunized with 50 microg of GST-Iss died when challenged with the O78 strain. No other deaths occurred. Immunized chickens produced a serum and mucosal antibody response to Iss and had significantly lower lesion scores than nonimmunized chickens following challenge, regardless of the challenge strain. This study expands on our previous report of the value of Iss as an immunoprotective antigen and demonstrates that immunization with Iss can provide significant protection of chickens against challenge with three different E. coli strains.  相似文献   

Magnetic resonance imaging findings of hepatic encephalopathy in a dog with a portosystemic shunt     

Moon SJ  Kim JW  Kang BT  Lim CY  Park HM 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(3):361-366

A 6-year-old ShihTzu presented with tonic-clonic cluster seizure. T2-weighted magnetic resonance (MR) images showed bilateral diffuse hyperintense lesions at the cerebral cortex with enlarged sulci. Computed tomography revealed a portosystemic shunt (PSS) and azygos continuation. Based on the clinical signs, blood examinations and diagnostic images, the dog was diagnosed with hepatic encephalopathy secondary to PSS. The neurologic signs were gradually improved after medical therapy for hyperammonemia. This is the first report of hyperintensity of the cerebral cortex on T2-weighted MR images associated with acute hepatic encephalopathy in a dog.  相似文献   

The effects of replacement of antibiotics with by‐products of oriental medicinal plants on growth performance and meat qualities in fattening pigs     

Suk Nam KANG  Gyo Moon CHU  Young Min SONG  Sang Keun JIN  In Ho HWANG  Il Suk KIM 《Animal Science Journal》2012,83(3):245-251

The effect of by‐products of oriental medicinal plants (OMP; T1) containing 0.03% herb extracts (T2) or 0.1% aminolevulinic acid (T3) on the production performance of swine during the finishing period and on its meat quality were investigated. No significant differences were found in the weight gain, feed intake and feed conversion rate among the tested groups (P > 0.05). But the treated group showed higher (P < 0.05) moisture and ash and lower protein than the control group. The T3 group showed a lower meat cholesterol content (38.42 mg/100 g) compared to the other groups (P < 0.05). The vitamin E content of the muscle in the treated groups was higher compared to the control group. No antibiotic content was detected in all treated and control samples. The values of the volatile basic nitrogen (VBN) and thiobarbituric acid reactive substance (TBARS) of the treated groups were significantly lower (P < 0.01) than the control group. The treated groups had significantly better (P < 0.05) sensory‐test scores for color, flavor, off‐flavor and total acceptability compared to the control group.  相似文献   

Modulatory effects of functional rice cultivars giant embryo and Aranghyangchal on the body weight and lipid metabolism in mice fed with a high fat diet   总被引：1，自引：0，他引：1  

Mi Young Kang  Jung Eun Moon  Sang Chul Lee 《Journal of Crop Science and Biotechnology》2013,16(2):167-171

The effects of the functional rice cultivars giant embryo and Aranghyangchal on the body weight and lipid metabolism in male C57BL/6N mice under high fat diet conditions were investigated. The animals were randomly placed into four dietary groups: normal control (NC group), high fat (HF group), and high fat supplemented with giant embryo (HF-GE group) or Aranghyangchal (HF-AR group). After 8weeks, a marked increase in the body weight and plasma triglyceride and total cholesterol concentrations were found in the HF mice relative to the control group. However, the addition of the functional rice samples in the high fat diet reduced the body weight gain and significantly decreased the lipid levels through inhibition of lipogenesis and regulation of adipokine production. The results demonstrate that the functional rice grains giant embryo and Aranghyangchal may both be useful as biomaterials in the development of functional food with therapeutic potential against high fat diet-induced hyperlipidemia.  相似文献   

First Report on Molecular Detection of Equine Upper Respiratory Infectious Viruses in Republic of Korea     

Sungjin Ko  Jun-Gu Kang  Jung-Yong Yeh  Jin-San Moon  Gui-Cheol Choi  Sohyun Won  Joon-Seok Chae 《Journal of Equine Veterinary Science》2013

The prevalence of equine respiratory virus infections among a suspected population of race horses was examined using polymerase chain reaction (PCR). One or more of five equine respiratory viruses were detected in the nasal swabs of 45 of 89 horses (50.6%), and the detection rate of equine herpesvirus type 1 (EHV-1), equine herpesvirus type 4 (EHV-4), equine herpesvirus type 5 (EHV-5), equine rhinitis A virus (ERAV) and equine rhinitis B virus (ERBV) were 5.6%, 7.9%, 39.0%, 2.2%, and 6.7%, respectively. Among the 45 infected horses, 7 were co-infected with EHV and/or equine rhinitisvirus (ERV). Equine influenzavirus and equine arteritisvirus were not detected in any samples. Specific antibodies to EHV-1 and/or EHV-4 were detected in 59 of 73 tested sera (80.8%), using a virus neutralization test. This investigation suggests that equine respiratory viruses are endemic at Seoul Race Park and that the impact of viral infections on race horses’ health in Republic of Korea should be evaluated.  相似文献   

Presumptive Nocardia spp. infection in a dog treated with cyclosporin and ketoconazole     

AEH Paul  CS Mansfield  M Thompson 《New Zealand veterinary journal》2013,61(5):265-268

Abstract

CASE HISTORY: A dog that had received 8 months of cyclosporin and ketoconazole therapy for treatment of atopic dermatitis subsequently developed severe neurological disease, that failed to respond to treatment with trimethoprim-sulphadiazine and clindamycin.

HISTOPATHOLOGICAL FINDINGS: Histopathological examination of the pulmonary parenchyma and spinal cord revealed loose aggregates of Gram-positive, partially acid-fast, fine, beaded, filamentous bacteria, most consistent with Nocardia spp.

DIAGNOSIS: A presumptive diagnosis was made of disseminated nocardiosis of the spinal cord and lungs.

CLINICAL RELEVANCE: Nocardia spp. is an opportunistic actinomycete that may cause disseminated disease, particularly in immunocompromised animals. Cyclosporin is used in veterinary medicine to control immune-mediated and allergic disorders, with few reported adverse side effects. This case gives further evidence that involvement of the spinal cord in nocardiosis of the central nervous system (CNS) carries a poor prognosis, and opportunistic infection by Nocardia spp. may be a potential complication of immunosuppressive cyclosporin therapy in the dog.  相似文献   

Effects of intensification of pastoral farming on greenhouse gas emissions in New Zealand     

CS Pinares-Patiño  GC Waghorn  RS Hegarty  SO Hoskin 《New Zealand veterinary journal》2013,61(5):252-261

Abstract

AIMS: The aims of this cross-sectional study were to investigate the herd and cow-level prevalence of bovine digital dermatitis (BDD) in dairy farms in the northern Taranaki region of New Zealand, and to identify whether there was any spatial clustering of herds with the disease.

METHODS: A survey of 224 dairy farms in the northern Taranaki region of New Zealand was undertaken from September 2014 to February 2015. Following training in robust criteria to confirm BDD visually, a technician inspected the rear feet of every milking cow on the farms during milking. The identity of cows with lesions and the feet involved were recorded. The proportion of cows affected among the inspected population (cow-level prevalence), the proportion of a herd affected (farm-level prevalence), and proportion of farms with ≥1 cow with lesions, were calculated. A bivariate K function analysis was then used to assess whether farms with ≥1 cow with lesions were clustered, after accounting for the distribution of the farms involved in the study.

RESULTS: Bovine digital dermatitis lesions were observed on 143/224 (63.8 (95% CI=57.5–70.1)%) farms. Within-farm prevalence was 0% on 81 (36.2%) farms, between >0 and <3% on 120 (53.5%) farms, with a maximum prevalence of 12.7% on one farm. Overall, cow-level prevalence was 707/60,455 (1.2 (95% CI=0.9–3.0)%), and on affected farms was 707/41,116 (1.7 (95% CI=1.4–2.1)%). In affected cows, 268/707 (37.9%) had a lesion on left foot only, 262/707 (37.1%) on the right foot only and 177/707 (25.0%) on both feet. The K function analysis showed no evidence of clustering of farms with BDD.

CONCLUSIONS: Bovine digital dermatitis was widespread among the survey farms, but there was no evidence that there was any clustering of herds with BDD. The cow-level prevalence on affected farms was much lower than reported elsewhere.

CLINICAL RELEVANCE: Although the prevalence at the cow level was low, if these data are representative of other regions of New Zealand, BDD could easily become a major problem on dairy farms in New Zealand, as has been observed in other countries.  相似文献