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111.
112.
Despite the substantial interest to ecologists of the relationship between species diversity and ecosystem functioning, little is known about how the high species richness of decomposer (saprotrophic) fungi and their relative frequencies of occurrence influence the decomposition of organic matter. Three experiments were conducted to test the ability of culturable saprotrophic fungal isolates to utilise a range of artificial and more natural substrates that occur in organic matter, with the aims of (1) characterising the functional potential of ‘common’ and ‘occasional’ taxa in an upland grassland soil and (2) determining whether there was a high degree of apparent functional redundancy in these communities. ‘Function’ was defined as the ability of a fungal isolate to utilise broad categories of substrates (e.g. sugars, cellulose, lignin) that occur in organic matter and which change in proportion during decomposition. The terms ‘common/abundant/frequent’ and ‘occasional/infrequent’ usually referred here to the frequencies of occurrence of taxa estimated using Warcup soil plates. Accepting the difficulties of sampling fungi in soil, this appeared to be the most useful isolation method to produce a general picture of the microfungal community with an estimate of frequency of occurrence for every taxon obtained, and to provide cultures for use in function tests. The influence of this technique on the interpretation of the results is discussed.Forty-eight fungal isolates, obtained from an upland grassland in Roxburghshire, UK, were selected to cover the most ‘abundant’ taxa and a range of ‘occasionals’. Pure cultures of anamorphic fungi and members of the Zygomycota, Ascomycota and Basidiomycota were tested. Although there was apparently a high degree of functional redundancy (equivalence) in assemblages of culturable decomposer fungi, with ‘frequent’ and ‘infrequent’ taxa largely utilising the same substrates, the ‘infrequent’ taxa played important roles in decomposition. ‘Infrequent’ microfungi tested were potentially more active in decomposition than the ‘frequent’ taxa, i.e. several had a higher overall activity, were able to utilise a wider range of substrates and were more combative than the ‘abundant’ taxa. When ‘abundant’ and ‘occasional’ taxa from the same putative guild were inoculated together on grass litter, there was slight evidence of ‘positive’ indirect effects on decomposition and cellulose degradation. Some ‘negative’ effects on lignin degradation, probably as a result of combat, were observed.It is possible that the ‘occasional’ taxa increased the temporal resilience of the ecosystem process of decomposition, and were ‘waiting in the wings’ to replace the abundant taxa. Nevertheless, greater functional diversity could be associated with the uncultured taxa not studied here.  相似文献   
113.
The effect of temperature and duration of cooking on plantain and banana fruit texture and cytpoplasmic and cell wall components was investigated. The firmness of both banana and plantain pulp tissues decreased rapidly during the first 10 min of cooking in water above 70 degrees C, although plantain was much firmer than banana. Cooking resulted in pectin solubilzation and middle lamella dissolution leading to cell wall separation (as observed by SEM). Dessert banana showed more advanced and extensive breakdown than plantain. Although dessert banana had a higher total pectin content than plantain, the former had smaller-sized carboxyethylenediaminetetraacetic acid (CDTA) soluble pectic polymers which are associated with plant tissues that have a propensity to soften. Plantain had higher levels of starch and amylose than banana but this was associated with a firmer fruit texture rather than a softening due to cell swelling during starch gelatinization. Different cooking treatments showed that cooking in 0.5% of CaCl(2) solution and temperatures below 70 degrees C had significant effects on maintenance of pulp firmness.  相似文献   
114.
To document the roles of farm women in the contemporary farm economy, their physical health and psychological well-being, and their interaction with and evaluation of the health care system, a multi-stage study employing both quantitative and qualitative research strategies was implemented. A total of 717 Canadian farm women in 20 rural Saskatchewan municipalities returned a 20-page objective questionnaire that focused on: (1) health care, (2) health status, (3) social support, (4) well-being, (5) lifestyle and activities, (6) stress, (7) work, (8) male and female roles, (9) demographics, and (10) farm issues. Subsequently, 20 qualitative interviews were conducted to explore in-depth the findings of the survey. Results document long hours of work, on and off the farm, that often went unacknowledged; pressure to assume the role of a "traditional farm wife," expectations they often felt they had difficulty living up to; and high levels of stress in response to economic and family pressures. Although rating the availability and quality of health care as "fair to good," the farm women commented on the lack of access to medical and counseling services, and a perceived lack of understanding by policymakers and professionals. Integrated health and educational service centers, increased use of nurse practitioners, and establishment of mobile health services are recommended policy initiatives.  相似文献   
115.
The effect of inulin on isoflavone absorption and the effect of isoflavones and synergy with inulin on calcium absorption in rats was investigated. Rats (n = 48) were divided into three groups and fed inulin (50 mg/g), isoflavone (8 mg/g) or inulin + isoflavone (50 mg/g + 8 mg/g) diets for 21 days. After a 2-h fast, rats were given (45)Ca orally or intraperitoneally, together with 25 mg of calcium as calcium acetate. Blood and femurs were collected 4 days later. Sera were analyzed for isoflavones using HPLC-MS, femurs for (45)Ca by beta-scintillation counting, and total femoral calcium by atomic absorption spectroscopy. Both groups fed isoflavones had similar and significantly higher weight-adjusted total femoral calcium content compared to the inulin-fed group (p < 0.0001). (45)Ca absorption was significantly higher (p < 0.01) when isoflavones were added to the diet, and serum equol was significantly lower (p < 0.01) when inulin was added to the diet containing isoflavones. We conclude that isoflavones enhance calcium absorption, without synergy from inulin, and that inulin decreases equol production.  相似文献   
116.
A liquid chromatographic method for the determination of coumarin anticoagulants in tablets was collaboratively studied by 7 laboratories. The method uses an octadecylsilane-bonded microparticulate column, tetrahydrofuran-methanol-water-acetic acid mobile phase, and photometric detection at 311 nm. Each collaborator received samples of warfarin sodium, phenprocoumon, and dicumarol as a synthetic composite and as commercial individual and composited tablets. Pooled average assay values for synthetic and commercial tablet samples of warfarin sodium were 101.6 and 99.5%, respectively, with a combined reproducibility SD of 2.38% (CV = 2.37%) and combined repeatability SD of 1.49% (CV = 1.49%). Pooled average (SD) assay values for dicumarol and phenprocoumon commercial samples were 98.0 (2.27) and 101.3% (4.00), respectively. The content uniformity determinations of 2 mg warfarin sodium and 25 mg dicumarol tablets indicated average tablet contents (range) of 99.5% (91.0-116.0) and 98.0% (89.8-108.8), respectively. The method has been approved interim official first action.  相似文献   
117.
Gene activation     
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118.
Moore RE 《Science (New York, N.Y.)》1968,161(3848):1358-1359
Ancient mosaic patterns tend to be certain absolute sizes (mosaic units). There is evidence that the mosaicists had these lengths marked on rulers. A ruler such as one which an ancient mosaicist might have used has been reconstructed. It is possible that an original may still exist.  相似文献   
119.
120.
Chlorinated waters are being introduced into estuarine and coastal areas in increasing quantities. In such systems, the chlorine reacts with the natural bromide and ammonia to produce the highly toxic hypobromous acid, hypobromite ion, and haloamines. Sunlight causes up to 50 percent conversion to bromate ion, which is persistent in natural waters and has an unknown toxicity.  相似文献   
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