Risk factors for transmission of foot-and-mouth disease during an outbreak in southern England in 2007     

Ellis-Iversen J  Smith RP  Gibbens JC  Sharpe CE  Dominguez M  Cook AJ 《The Veterinary record》2011,168(5):128

During an outbreak of foot-and-mouth disease (FMD) in southern England in 2007, a case-control study was conducted to identify risk factors for infection and to investigate the relative impact of risk factors on transmission between the infected farms. Seven of the eight case farms in the outbreak and 22 control farms participated. Data were collected via questionnaires and subjected to comparative statistical analysis. Case farms were further classified as primary or secondary according to the likely source of infection during the study. On primary case farms, it was plausible that infection had been introduced directly from the original source. On secondary case farms, FMD infection was more likely to have originated from another infected premises. Calving occurred more frequently on case farms than on control farms during the risk period, and the two primary case farms had a larger proportion of youngstock than the other farms. Secondary case farms (n=5) had a higher composite environmental risk score and a lower biosecurity score than control farms.  相似文献   

Validation of a 2-mm videoendoscope for the evaluation of the paranasal sinuses with a minimally invasive technique     

Morgane Pouyet DMV  Alvaro G. Bonilla DMV  MS  DACVS 《Veterinary surgery : VS》2020,49(Z1):O60-O70

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Ultrasound-Assisted Water Extraction of Mastocarpus stellatus Carrageenan with Adequate Mechanical and Antiproliferative Properties     

Maria Dolores Torres  Noelia Flrez-Fernndez  Herminia Dominguez 《Marine drugs》2021,19(5)

Ultrasound-assisted water extraction was optimized to recover gelling biopolymers and antioxidant compounds from Mastocarpus stellatus. A set of experiments following a Box–Behnken design was proposed to study the influence of extraction time, solid liquid ratio, and ultrasound amplitude on the yield, sulfate content, and thermo-rheological properties (viscoelasticity and gelling temperature) of the carrageenan fraction, as well as the composition (protein and phenolic content) and antiradical capacity of the soluble extracts. Operating at 80 °C and 80 kHz, the models predicted a compromise optimum extraction conditions at ~35 min, solid liquid ratio of ~2 g/100 g, and ultrasound amplitude of ~79%. Under these conditions, 40.3% carrageenan yield was attained and this product presented 46% sulfate and good mechanical properties, a viscoelastic modulus of 741.4 Pa, with the lowest gelling temperatures of 39.4 °C. The carrageenans also exhibited promising antiproliferative properties on selected human cancer cellular lines, A-549, A-2780, HeLa 229, and HT-29 with EC₅₀ under 51.9 μg/mL. The dried soluble extract contained 20.4 mg protein/g, 11.3 mg gallic acid eq/g, and the antiradical potency was equivalent to 59 mg Trolox/g.  相似文献   

Ontogeny of digestive tract functionality in Japanese flounder, Paralichthys olivaceus studied by in vivo microinjection: pH and assimilation of free amino acids     

I. Rønnestad  R. Pérez Dominguez  M. Tanaka 《Fish physiology and biochemistry》2000,22(3):225-235

To specifically study the functional ontogeny of the digestive tract, larvae of Japanese flounder at various developmental stages were injected with liquid solutions using tube feeding in vivo. The survival in the stages tested was on average 93%. The injected solutions were almost completely transferred from the presumptive stomach, or stomach to the midgut within 10 min of injection. The passage to the hindgut in some cases started 10 min after injection and over 90% of the solution had passed from the midgut to the hindgut after 1 h. In most cases the hindgut seemed to be completely empty after 3 h.Two different mixtures of pH indicators with sensitivities in the alkaline (7.5 to 9) and acid (4 to 6) ranges respectively were used for assessment of pH in the various gut segments. The pH of the stomach remained alkaline during the larval period, but had fallen close to 4 during late metamorphosis, an indication of active HCl secretion and progressive stomach differentiation. In mid and late metamorphosing fish a rapid colour change in the pH indicator was observed once it had passed the pyloric sphincter. This demonstrates that there was also active secretion of alkaline fluid, most likely HCO₃ ^–, from the pancreas into the pylorus lumen.A single injection of liquid solution of ¹⁴C-FAA showed that assimilation of FAA was high in all stages tested (79.5 ± 7.1%; SD; n=91). The presently reported data for Japanese flounder support earlier studies that FAA are absorbed with a high efficiency in the early stages of marine fish.  相似文献   

How climate change might affect tree regeneration following fire at northern latitudes: a review     

Boucher  Dominique  Gauthier  Sylvie  Thiffault  Nelson  Marchand  William  Girardin  Martin  Urli  Morgane 《New Forests》2020,51(4):543-571

New Forests - Climate change is projected to increase fire severity and frequency in the boreal forest, but it could also directly affect post-fire recruitment processes by impacting seed...  相似文献   

Analytic validation of commercially available immunoassays for the measurement of serum cobalamin and folate concentrations in pigs          下载免费PDF全文

Niels Grützner  Bruce D. Lawhorn  Brandon Dominguez  Johannes Kauffold  Jan S. Suchodolski  Jörg M. Steiner 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(2):311-319

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Use of an axial pattern flap and nictitans to reconstruct medial eyelids and canthus in a dog     

Susan Jacobi  Bryden J. Stanley  Simon Petersen-Jones  Nikolaos Dervisis  Pedro A. Dominguez 《Veterinary ophthalmology》2008,11(6):395-400

A 10-year-old male neutered Boxer presented with recurrence of a mast cell tumor at the right medial canthal area. Following excision including 2 cm margins, the medial one-half of the upper and lower eyelids and the medial canthus were reconstructed using an axial pattern flap based on the cutaneous branch of the superficial temporal artery. The bulbar conjunctiva of the nictitans was preserved and sutured to the medial flap edge, thus creating a conjunctival lining to the deep aspect of the flap, protecting corneal epithelium. This is a valuable surgical technique for closing a large skin defect and reconstructing the medial eyelids, thus preserving the globe.  相似文献   

Predictors of urinary or fecal incontinence in dogs with thoracolumbar acute non‐compressive nucleus pulposus extrusion     

Lorenzo Mari  Sebastien Behr  Anita Shea  Elisabet Dominguez  Cristoforo Ricco  Emili Alcoverro  Abel Ekiri  Daniel Sanchez‐Masian  Luisa De Risio 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(6):2693-2700

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Causes, characteristics and consequences of anovulatory follicles in superovulated sheep     

Veiga-Lopez A  Gonzalez-Bulnes A  Tresguerres JA  Dominguez V  Ariznavarreta C  Cocero MJ 《Domestic animal endocrinology》2006,30(2):76-87

Efficiency of superovulatory protocols is affected by the occurrence of reproductive abnormalities, such as the presence of anovulatory follicles. The objective of current study was to assess the incidence and possible causes of anovulatory follicles in superovulated sheep, in order to characterize the endocrine functionality of these follicles in terms of estradiol production and to evaluate their relationship with development of embryos from other follicles. The number and size of all follicles present in the ovaries of 12 sheep treated with a superovulatory FSH step-down treatment was assessed by ultrasonography. On Day 3 after subsequent estrus behaviour, the number of corpora lutea and anovulatory follicles were recorded and the fluid of anovulatory follicles >or=5mm in size was aspirated and assayed for estradiol. At once, embryos were recovered to evaluate their viability. In current study, anovulatory structures averaged 34.6% of the follicles developing to preovulatory sizes. The number of anovulatory follicles was determined by the existence of follicular dominance effects, since they increased with a higher difference in size between the largest and the second largest follicle at the beginning of the superovulatory treatment (P<0.05, r(2)=0.420). Most of the anovulatory follicles showed signs of functionality failures, indicated by a low mean estradiol concentration (9.9+/-1.1 ng/ml). However, a 22.4% of them were highly estrogenic (>200 ng/ml) and their permanence beyond the ovulation was related to a drop in the embryo viability rate (P<0.005), leading to decreased final superovulatory yields.  相似文献   

Elite durum wheat genetic map and recombination rate variation in a multiparental connected design     

Laurence Vaissayre  Morgane Ardisson  Christiane Borries  Sylvain Santoni  Jacques David  Pierre Roumet 《Euphytica》2012,185(1):61-75

Genetic maps published for elite durum wheat (Triticum turgidum durum Desf.), were mainly related to Italian and North American pools. To provide additional information dedicated to durum elite pool, a recombinant inbred population (217 RILs) derived form 2 French commercial varieties (Ixos and Primadur), has been mapped with 529 loci (98 AFLPs, 67 SSRs, 1 ISBP and 363 DArTs). Genetic map spanned a total of 2,082 cM (5.7 cM/marker on average). Recombination rate variation over the genome was documented through the analysis of six segregating populations representing a total of 48 RILs × 6, issued from an half diallel design based on four elite lines—Neodur, Ixos, Lloyd and Primadur—including the 2 previous genitors. Each set of three genetically connected populations (48 RILs × 3) was used to build the consensus parental maps for each genitor. To construct the four consensus parental maps, marker ordering was determined on 217 RILs map which was an extended population of the most polymorphic cross (Ixos × Primadur). Sizeable, recombination rate variation has been observed between the four parental maps both at the marker pair scale and at the linkage group scale. Two of the parents, Lloyd and Primadur, had strong, opposite effects increasing and decreasing, respectively the recombination rate. Additional studies are proposed to increase our understanding of this variation, with the identification of environmental or genetic factors affecting recombination rate.  相似文献