Histochemical study of the intestinal absorption,liver and lens effect with zinc‐supplemented diets for gilthead seabream     

Pedro L. Castro  Rafael Gins  David Dominguez  Elvira Rey  Lidia Robaina  Vasileios Karalazos  Marisol Izquierdo 《Aquaculture Nutrition》2019,25(1):66-77

To describe the effect and the distribution of zinc in different tissues of a representative marine fish species, gilthead seabreams (Sparus aurata Linnaeus, 1758) were fed different diets containing zinc in different sources (organic, inorganic, encapsulated, blood‐rich diet or fishmeal). The effect was monitored by histology in the lens, liver and intestine. In addition, the anterior and posterior intestine was studied by means of autometallography, a histochemical silver‐based staining method to determine the zinc flow and distribution. The histology of lens did not improve by dietary zinc as there was no occurrence of cataracts. In the liver, the experimental diets were correlated with different changes in the tissue architecture. In the intestine, no histological changes were found using haematoxylin and eosin stain. However, with the use of autometallography stain, it was possible to visualize and describe the route that zinc follows through the enterocytes and the lamina propria. Organic and inorganic zinc diet produced the richest silver deposition in the anterior intestine.  相似文献   

Effects of changes in environmental factors on the non-specific immune response of Nile tilapia, Oreochromis niloticus L.     

Miriam Dominguez  Akihiro Takemura  & Makoto Tsuchiya 《Aquaculture Research》2005,36(4):391-397

Lysozyme acts as a non‐specific defence substance and is found in the peripheral blood, cutaneous mucus and certain tissues of marine and freshwater fishes. In the present study, we examined the effect of various environmental factors (water temperature, salinity, pH and suspended sediments) on plasma lysozyme activity in the Nile tilapia, Oreochromis niloticus L. When the fish were reared at different water temperatures (18.4, 23, 28 and 33°C), plasma lysozyme activity increased at 28°C after 2 and 4 weeks. A significant decrease in lysozyme activity was found in the fish reared at 33°C for 4 weeks. These results suggest that there is a water temperature range that affects the amount of plasma lysozyme activity that can be detected. Fish cultured at 24 g L^?1 of salinity for 2 and 4 weeks and 12 g L^?1 for 4 weeks resulted in significantly increased plasma lysozyme activity, suggesting that environmental salinity also affects the amount of plasma lysozyme that can be detected. Lysozyme activity also significantly increased when the fish were held in acidic water at pH 4.0 and in suspended sediments at 2000 mg L^?1 for 2 weeks. It was concluded that changes in some aquatic environmental factors affect the non‐specific immune responses of Nile tilapia.  相似文献   

An experimental model for calcium carbonate urolithiasis in goats          下载免费PDF全文

Meredyth L. Jones  Brandon J. Dominguez  Michael A. Deveau 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):1268-1273

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Effects of management regimes and extreme climatic events on plant population viability in Eryngium alpinum     

Marco Andrello  Jean-Philippe Bizoux  Morgane Barbet-Massin  Myriam Gaudeul  Florence Nicolè  Irène Till-Bottraud 《Biological conservation》2012,147(1):99-106

Extreme climatic events like the 2003 summer heatwave and inappropriate land management can threaten the existence of rare plants. We studied the response of Eryngium alpinum, a vulnerable species, to this extreme climatic event and different agricultural practices. A demographic study was conducted in seven field sites between 2001 and 2010. Stage-specific vital rates were used to parameterize matrix population models and perform stochastic projections to calculate population growth rates and estimate extinction probabilities. Among management regimes, spring grazing and land abandonment decreased vital rates and population growth, while autumn grazing and late mowing had positive effects on population viability. The 2003 heatwave reduced fecundity rates and survival rates. Only spring grazed sites presented considerable extinction risk. Stochastic projections showed that an increased frequency of 2003-like events may exacerbate extinction risk, but extinction probability depends mainly on land management regimes. To better conserve E. alpinum populations, we recommend conversion of presently spring grazed and abandoned sites to late mowing or autumn grazing.  相似文献   

Immunomodulatory effect of swine CCL20 chemokine in DNA vaccination against CSFV     

Tarradas J  Álvarez B  Fraile L  Rosell R  Muñoz M  Galindo-Cardiel I  Domingo M  Dominguez J  Ezquerra A  Sobrino F  Ganges L 《Veterinary immunology and immunopathology》2011,142(3-4):243-251

The objective of this work was to explore whether a plasmid expressing CCL20 chemokine could improve the immune response against CSFV in co-administration with a DNA vaccine expressing the E2 protein. The immunization of pigs with the DNA vaccine formulation, that contains swine CCL20 chemokine, resulted in the homogenous induction of detectable levels of CSFV antibodies at 36 days after the first injection. Remarkably, immunized animals with E2 DNA vaccine in co-administration with the plasmid containing swine CCL20 developed high titers of neutralizing antibodies against homologous and heterologous CSFV strains and were totally protected upon a lethal viral challenge (sterilizing protection). Our results confirm the role of CCL20 to increase antibody-mediated responses. At the same time suggest the ability of CCL20 to enhance the T helper cell response associated with the induction of neutralizing antibodies against CSFV in pigs previously reported. Systemic replication of virulent CSFV in vivo during the acute phase of infection induces type I IFN. Lower average values of IFN alpha were detected in the serum of pigs immunized with pE2 and pCCL20 at 3 days after challenge. The levels of IFN-alpha detected in pigs immunized with pE2 and principally in non-vaccinated challenged animals can be related to viral load in serum at 3 and 7 days post infection and the clinical signs observed. Our results emphasized the capacity of swine CCL20 chemokine to enhance cellular, humoral and anti viral response with an adjuvant effect in the immune response elicited by E2-DNA vaccination against CSFV. To our knowledge, this is the first report demonstrating the adjuvant effect of swine CCL20 to effectively enhance the potential of DNA vaccine in the immune induction and protection against virus challenge in swine infection model.  相似文献   

Genetical studies of hullability in comparison with other sunflower seed characteristics     

Liliane Denis  Juan Dominguez  Mario Baldini  Felicity Vear 《Euphytica》1994,79(1-2):29-38

Summary To study the genetic control of hullability in sunflower (Helianthus annuus L.), 36 hybrids, produced by a factorial cross of six male sterile and six restorer lines, were evaluated in two locations in Spain and one in France. Hullability was calculated as the ratio of the quantity of hull removed by a mechanical huller compared with total hull content. In dry conditions in Spain, hullability was twice (83.1%) that observed in France (41.5%). In all locations, male, fernale and interaction effects were significant. Estimates of narrow sense heritability varied between 0.78 and 0.82, values similar to those for other seed characters such as oil content. There were positive genetic and phenotypic correlations between hullability and 1000 seed weight (means 0.81 and 0.74, respectively) but negative correlations with seed oil content (–0.73 and –0.68, respectively). It is concluded that selection in early segregating generations of crosses involving good hulling material needs a specific strategy in order to maintain reasonable levels of oil and protein contents in the seed of the selected genotypes.  相似文献   

Determination of aflatoxin M1 in milk and milk products contaminated at low levels   总被引：1，自引：0，他引：1  

L Dominguez  J L Blanco  E Gomez-Lucia  E F Rodriguez  G Suarez 《Journal of the Association of Official Analytical Chemists》1987,70(3):470-472

A new method is described for the determination of aflatoxin M1 in milk and dairy products by thin layer chromatography. The main characteristic is the extraction system using an alkaline solution. Lipids are removed by centrifuging at low temperatures, and the aflatoxins are then extracted with CHCl3. The method has 2 options: Technique II (detection limit 0.02 ppb) requires cleanup on a chromatographic column; this is not necessary in Technique I (detection limit 0.1 ppb). The recovery rate in both techniques is over 92.8% in milk and yoghurt. This method may also be used for other aflatoxins. Because of the advantages of the method, Technique II is recommended for aflatoxin M1 control in milk, where a low detection limit is necessary. Technique I is proposed for experimental aflatoxin production studies in dairy products, which require analysis of a large number of samples but which do not require a very low detection limit.  相似文献   

Epidemiologic investigation of a silage-associated epizootic of ovine listeric encephalitis, using a new Listeria-selective enumeration medium and phage typing.     

J A Vázquez-Boland  L Dominguez  M Blanco  J Rocourt  J F Fernández-Garayzábal  C B Gutiérrez  R I Tascón  E F Rodriguez-Ferri 《American journal of veterinary research》1992,53(3):368-371

The role of silage feeding in the origin of an epizootic of encephalitic listeriosis in a sheep flock was investigated by use of a new direct Listeria-selective isolation and enumeration medium, in combination with serotyping and phage typing. The silage contained high numbers (about 10(6) cells/g) of a L monocytogenes strain indistinguishable with respect to serovar and phagovar from that isolated from the brains of sick sheep. These results provided unambiguous bacteriologic evidence of the epidemiologic link between silage consumption and listeriosis in ruminants.  相似文献   

Phalloides Syndrome Poisoning After Ingestion of Lepiota Mushrooms     

Morgane Kervégant  Luc de Haro  Anne Marie Patat  Charlotte Pons  Laurent Thomachot  Philippe Minodier 《The Forestry Chronicle》2013,24(2):170-172

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Combined Gemcitabine and Carboplatin Therapy for Carcinomas in Dogs     

P.A. Dominguez  N.G. Dervisis  C.D. Cadile  L. Sarbu  B.E. Kitchell 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(1):130-137

Background: Response and adverse reactions to combined gemcitabine (GEM) and carboplatin (CARBO) therapy in dogs with carcinomas are not documented.
Hypothesis: GEM and CARBO are safe for the treatment of dogs with carcinomas.
Animals: Thirty-seven dogs with histologically or cytologically confirmed carcinomas.
Methods: Prospective clinical trial. Dogs were treated with GEM (2 mg/kg, 20–30-minute infusion IV) on Days 1 and 8 and 4 hours later, CARBO (10 mg/kg IV) on Day 1. The cycle was repeated on Day 22.
Results: Thirty-seven dogs (29 with measurable tumor) received a median of 2 cycles (range 0.5–6) for a total of 101 cycles administered. Twelve dogs (32%) developed neutropenia (3 Grade 3, and 5 Grade 4) and 9 (24%) thrombocytopenia (2 Grade 3, and 1 Grade 4). Dogs >20 kg were twice as likely to develop thrombocytopenia ( P = .023). Twenty-seven dogs (73%) had evidence of gastrointestinal (GI) toxicosis, but most signs were of mild to moderate severity and self-limiting. One dog died of treatment-related complications. Overall tumor response rate was 13%. One dog with metastatic prostatic carcinoma achieved a complete remission and 1 dog with intestinal adenocarcinoma and 1 with tonsillar squamous cell carcinoma achieved partial remission. Twelve dogs achieved stable disease for a median of 72 days.
Conclusion and Clinical Importance: GEM and CARBO combination causes mild to moderate hematologic and GI toxicosis in dogs with carcinoma. Response rate in this study was modest, and optimization of dosing of this combination is required.  相似文献