Antiradical Capacity and Induction of Apoptosis on HeLa Cells by a <Emphasis Type="Italic">Phaseolus vulgaris</Emphasis> Extract     

Aparicio-Fernández X  Reynoso-Camacho R  Castaño-Tostado E  García-Gasca T  González de Mejía E  Guzmán-Maldonado SH  Elizondo G  Yousef GG  Lila MA  Loarca-Pina G 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(1):35-40

Jamapa bean is a black Phaseolus vulgaris variety rich in condensed tannins, anthocyanins and flavonols with interesting biological activities. The objective of this work was to evaluate the antiradical capacity (ARC) of a Jamapa bean methanolic extract (BME) and some of the proanthocyanidin-rich fractions derived from it, using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The effect of the BME on some proteins involved in apoptosis on HeLa cells was also evaluated. A strong correlation between proanthocyanidin concentration in BME and antiradical capacity was found, suggesting that these compounds contribute significantly to antiradical activity. BME was a better radical scavenger than butylated hydroxytoluene (45.6 and 33.9% ARC at 400 μM, respectively). Two proanthocyanidin-rich fractions obtained after a preliminary separation of the BME using Toyopearl (TP4 and TP6) exhibited a higher antiradical activity than the parent extract. The treatment of HeLa cells with 35 μg BME/ml/24 h increased the expression of Bax and Caspase-3, pro-apoptotic proteins (6.13 and 1.2 times for Caspase-3 and Bax, respectively). The mechanism of action of some proteins involved in apoptosis was also evaluated, and the results suggest that black Jamapa bean could be an important source of polyphenolic compounds with potential biological use as antioxidant and anticancer agents.  相似文献   

Antioxidant and Antimutagenic Activities of Mexican Oregano (Lippia graveolens Kunth)     

Martínez-Rocha A  Puga R  Hernández-Sandoval L  Loarca-Piña G  Mendoza S 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(1):1-5

Free essential oil methanolic extracts from three different geographical populations of Lippia graveolens in México were screened for antioxidant and antimutagenic properties by the DPPH and Kado microsuspension assay, respectively. Total phenolic and flavonoid contents as well as HPLC identification and quantification of naringenin and rosmarinic acid were also carried out. In addition, a taxonomical phenetic analysis was performed. The L. graveolens extracts showed varying content of phenols and flavonoids. Significant concentration of rosmarinic acid was found for the first time in the species. All the extracts were capable of scavenging DPPH radicals in a concentration dependent fashion; the IC₅₀ values correlate with the phenolic content. None of the extracts was toxic to TA100 and TA98 strains at the concentrations tested; moreover, the extracts at a concentration equivalent to 200 μg of gallic acid inhibited a 39 and 30% the mutagenicity induced by 4-nitro-o-phenylenediamine and sodium azide, respectively. The results suggest that the Mexican oregano is a source of polar bioactive ingredients for the food industry.  相似文献   

Survey of Vitamin B<Subscript>6</Subscript> Content in Commercial Presentations of Table Olives     

López-López A  Montaño A  Cortés-Delgado A  Garrido-Fernández A 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(2):87-91

For nutritional purposes, a survey of the vitamin B₆ levels from a variety of commercial presentations of table olives was carried out, taking into account the three main processing types (Spanish-style, directly brined, and ripe olives). The analysis was performed by HPLC, following the official French method for vitamin B₆ determination in foodstuffs. In-house validation data for two commercial table olives showed that the method precision was good (coefficient of variation <6%) and recovery was quantitative (104% on average). There was a wide range of values for vitamin B₆ in table olives (0–69.3 μg/100 g edible portion). The highest mean content was found in directly brined olives (33.9 μg/100 g edible portion) followed by Spanish-style (14.4 μg/100 g) and ripe olives (4.3 μg/100 g). On average, samples of the Gordal and Carrasqueña cultivars showed the highest vitamin B₆ content in the case of Spanish-style olives, but in directly brined olives as well as in ripe olives the effect of cultivar was not statistically significant (p?相似文献   

Production of transgenic adult plants from clementine mandarin by enhancing cell competence for transformation and regeneration   总被引：3，自引：0，他引：3  

Cervera M  Navarro A  Navarro L  Peña L 《Tree physiology》2008,28(1):55-66

Genetic transformation of mature trees is difficult because adult tissues are recalcitrant to Agrobacterium tumefaciens infection and transformation and because transgenic mature events are less competent for regeneration. We have shown that reinvigoration allows manipulation of the vegetative phase to increase the potential for transformation and regeneration without loss of competence for flowering and fruiting. To produce transgenic plants from clementine mandarin (Citrus clementina hort. ex Tanaka), we optimized the conditions of the source material both ex vitro and in vitro. Grafting of mature buds on juvenile rootstocks in the spring and preventing multiple bud sprouting by removing all but one bud permitted selection of vigorous first flushes for in vitro culture. Use of additional virulence genes from A. tumefaciens to increase transformation frequency and optimization of culture media and conditions to enhance explant cell competence for T-DNA integration and organogenesis resulted in efficient and reliable transgenic plant production. Transformed regenerants from explants, cultured in media without antibiotics, were identified by a screenable marker (either beta-glucuronidase or green fluorescent protein (GFP)), creating the possibility of generating transgenic clementine plants without antibiotic resistance marker genes. Stable integration of foreign genes was demonstrated by Southern blot analysis, and expression of these foreign genes was confirmed by detection of GFP fluorescence in leaves, floral organs and fruits of the transgenic plants.  相似文献   

Response of Scots pine (Pinus sylvestris L.) and pubescent oak (Quercus pubescens Willd.) to soil and atmospheric water deficits under Mediterranean mountain climate     

Rafael Poyatos  Pilar Llorens  Josep Piñol  Carles Rubio 《Annals of Forest Science》2008,65(3):306-306

The physiological responses to water deficits of Scots pine (Pinus sylvestris L.) and pubescent oak (Quercus pubescens Willd.) were studied under Mediterranean mountain climate. Minimum leaf water potentials were ?3.2 MPa for oak and ?2.1 MPa for pine, with higher predawn values for pubescent oak. Relative sap flow declined in both species when vapour pressure deficit (D) went above ca. 1.2 kPa, but stomatal control was stronger for pine during the 2003 summer drought. P. sylvestris plant hydraulic conductance on a half-total leaf area basis (k _L,s?1) was 1.2–2.6 times higher than the values shown by Q. pubescens, and it showed a considerably steeper decrease during summer. Leaf-level gas exchange was positively related to k _L,s?1 in both species. Scots pine was more vulnerable to xylem embolism and closed stomata to prevent substantial conductivity losses. The results of this study confirm that pubescent oak is more resistant to extreme drought events.  相似文献   

Thinning intensity and growth response in SW-European Scots pine stands     

Miren del Río  Rafael Calama  Isabel Cañellas  Sonia Roig  Gregorio Montero 《Annals of Forest Science》2008,65(3):308-308

The effect of different thinning intensities on growth and yield was studied in Pinus sylvestris L. stands at the south-western limit of its distribution area (Central Spain), using five long-term thinning trials. Data were analysed collectively considering several factors (trial, block, plot and period) as random effects. Total volume and volume increment decreased with thinning intensity, this loss being more significant in the case of moderate and heavy thinning. No difference was found among treatments for total basal area or the increment in basal area. The results revealed an optimum basal area (Assmann’s definition) between 85 and 100% of the basal area in unthinned plots. Volume growth loss associated with heavy thinnings (reduction of 18% in volume increment) was smaller than that reported in Central and Northern European regions (greater than 25%). Height increment was not influenced by thinning, whereas dominant and quadratic mean diameter increments increased with the thinning intensity. The response of diameter growth to thinning was greater at younger ages (less than 50 y) and in medium-sized trees.  相似文献   

上半年京城地区木材市场总体向好     

林木 《国际木业》2008,(7):35-38

今年上半年以来,从京城地区以至放大到京津冀三角经济带的木材市场总体情况来看,是向上、向好的。特别是进入6月份,时间过半,而且北京已经正式进入夏季,京城城市和农村基本建设、生产,以及人民生活也都进入了火旺时期,本期木材市场的运作形势基本体现出需求旺盛,销售加快,价格行情升降并存的特点。  相似文献   

今冬京城地区木材制品市场行情冷气袭人     

京木 《国际木业》2008,38(12)

京城地区木材业界针对目前木材制品供销情况越来越冷的现实,普遍认为今年的冬天将是京城地区木材市场近些年来最为难过的季节。  相似文献   

美国木材在华销路下滑不畅     

靳木 《国际木业》2008,38(9):33-33

美国是日前世界上最大的木材生产国和出口国之一。该国生产的针叶材如花旗忪、黄忪,阔叶材品种如樱桃木、枫木、黑胡桃木、白橡木、红像木、白栓木、白蜡木等等在我国进口材市场上都有出售。但是近年来,美国对我出口的原木和锯材的数量及价值金额都呈萎缩趋势,这一点,  相似文献   

On the independence between clupeid stocks and their recruitment     

Pérez-Rodríguez  Juan Carlos  Gómez-Muñoz  Victor Manuel 《Fisheries Science》2023,89(1):11-19

The statistical independence between the reproductive stock and its corresponding recruitment to a fishery was tested for 43 stocks of clupeids in the Ransom Myers database by using the chi-squared test of a contingency table. The method was validated by simulating stock and recruitment (SR) contingency tables for data close to hypothetical SR curves that represent several known phenomena. The clupeid SR data were grouped by the criteria of percentile points to build the contingency tables. The independence test of the clupeid stocks was rejected in only 15 cases, thus the independence hypothesis was sustained in the remaining 28 cases. This demonstrates that fitting an SR model in these clupeid stocks is not justified because their abundance could be affected by several causes other than reproductive stock fluctuations.

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