Detection of Echinococcus multilocularis infection in a colony of cynomolgus monkeys (Macaca fascicularis) using serology and ultrasonography.     

Patrick Rehmann  Andrea Gr?ne  Bruno Gottstein  Jürg V?llm  Heinz Sager  Martin Janovsky  Luca N Bacciarini 《Journal of veterinary diagnostic investigation》2005,17(2):183-186

Five animals in a colony of cynomolgus monkeys (Macaca fascicularis) died or were euthanatized because of alveolar echinococcosis, during a period of 5 years. The remainder of the colony was screened for possible infection with Echinococcus multilocularis, using serology and ultrasonography. A total of 46 animals out of a group of 55 were examined. The presence of anti-Em2 antibodies analyzed with enzyme-linked immunosorbent assay was demonstrated in 3 monkeys. In 2 of these 3 monkeys, multilocular structures compatible with metacestodal cysts in the liver were identified, using ultrasonography. The presence of alveolar echinococcosis was subsequently confirmed at postmortem examination in 1 animal. The other animals are still alive. Two other monkeys were negative in the serological examination but had cystic structures in the liver, which were identified as bile duct cysts at postmortem examination in 1 animal. The other monkey is still alive. These findings suggest that serology for antibodies against the Em2 antigen may represent a useful method in identifying animals that might be infected with E. multilocularis and are therefore at risk of developing fatal alveolar echinococcosis.  相似文献   

Observations of Phytophthora spp. in Water Recirculation Systems in Commercial Hardy Ornamental Nursery Stock     

Karin Themann  Sabine Werres  R. Lüttmann  H.-A. Diener 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(4):337-343

Samples of water and sediment were taken from drains, reservoirs and wells from four commercial hardy ornamental nurseries with water recirculation systems. The samples were taken on seven different dates throughout a single year from August 1994 to July 1995. The samples were screened for Phytophthora species using five different methods: direct plating, three bait tests (using lupin seedlings, apples and Rhododendron leaves) and a DAS-ELISA (double-antibody sandwich enzyme-linked immunosorbent-assay) with two antisera. In the nurseries with old water recirculation systems, Phytophthora species were detected in the drains and in the reservoirs. In the nursery with a new recirculation system, the pathogens were only present in the drains. None of the water samples from wells in any of the nurseries were contaminated. Phytophthora species were present in the water as well as in the sediment samples from drains and reservoirs. They were detected in the water recirculation systems irrespective of the season. The number of isolates increased about sevenfold between late summer and spring. At least 12 different Phytophthora species were identified: some isolates were previously unrecorded species. The epidemiology of the pathogens in outdoor water recirculation systems as well as the importance of the results for commercial nurseries is discussed.  相似文献   

Plant parasitic nematodes of Tylenchida (Nematoda) associated with groundnut (Arachis hypogaea) fields in the Mediterranean region of Turkey     

İ. Kepenekci  G. Öztürk 《Phytoparasitica》2002,30(3):288-289

In a nematode survey of Tylenchida carried out in groundnut (Arachis hypogaea L.) growing areas in the Mediterranean region of Turkey, 15 species were determined as belonging to ten genera of eight families within the superfamilies Tylenchoidea, Dolichodoroidea, Hoplolaimoidea and Anguinoidea. Each of them constitutes a new record on groundnut in Turkey, andScutylenchus tumensis Skwiercz andPratylenchus brachyurus (Godfrey) are reported for the first time in the nematofauna of Turkey.  相似文献   

Die Biologische Kontrolle der Baumwollzikade Empoasca decipiens Paoli (Homoptera: Cicadellidae) in Gewächshausgurken     

Isa Bünger  Hans-Peter Liebig  Claus P. W Zebitz 《Gesunde Pflanzen》2002,54(3-4):105-110

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Wie repräsentativ sind die Stichprobenverfahren von landwirtschaftlichen Expertensystemen?     

Dipl.-Geogr. Gregor Spickermann  Prof. Dr. Lutz Plümer 《Gesunde Pflanzen》2003,55(8):222-227

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Effekte moderner Verfahren der Bodenbewirtschaftung auf die Aktivität epigäischer Raubarthropoden     

Christa Volkmar  Marita Lübke-Al Hussein  Thomas Kreuter 《Gesunde Pflanzen》2003,55(2):40-45

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Auswirkungen von Verpilzung und Mykotoxinbehaftung der Futtermittel auf Leistung und Gesundheit bei Schweinen und Rindern     

Klaus Hörügel  Christine Gebhart  Gudrun Hanschmann  Henriette Mietke 《Gesunde Pflanzen》2003,55(5):151-157

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Die Wirkung elektromagnetischer Stimulation auf den Keimungsprozess von Weizen     

Branko Marinkovic  Miroslav Maleševic  Jovan Crnobarac  Hans-Jürgen Schaller  Franz Götz  Olaf Röder  Goran Jacimovic 《Gesunde Pflanzen》2003,55(6):187-190

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Die Biophysik in der Wachstumsfunktion von jungen Maispflanzen     

Branko Marinkovic  Novica Petrovic  Jovan Crnobarac  Hans-Jürgen Schaller  Franz Götz  Olaf Röder  Goran Jacimovic 《Gesunde Pflanzen》2003,55(6):184-186

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袋鼠花的组织培养   总被引：8，自引：0，他引：8  

刘春  穆鼎 《园艺学报》2003,30(1):113-115

 对袋鼠花( Anigozanthos) 组织培养研究表明: 外植体消毒以0. 1 %升汞溶液浸泡, 7～8 min 为宜; 不同浓度激素对其愈伤组织形成、分化以及根的形成有不同的影响, 随着62BA 浓度的增加, 愈伤组织的诱导率有增加的趋势; 生长素与分裂素的比例决定着愈伤组织的分化; 小苗在1/ 2 MS 培养基上生根最好, 6-BA 对其生根有抑制作用。各培养阶段最适培养基: (1) 愈伤, MS + 6-BA 1 mg/L + NAA 0. 1 mg/L +3 %活性炭; (2) 芽诱导, MS + 6-BA 0. 5 mg/L + NAA 0. 5 mg/L ; (3) 生根, 1/ 2 MS + IBA 0. 2 mg/L。  相似文献