Application of Exogenous Esterified Hyaluronan to Equine Distal Limb Wounds     

Stefan H. Witte  Abayomi K. Olaifa  Andrew J. Lewis  Randy B. Eggleston  Jaroslava Halper  Manfred Kietzmann  Wolfgang Baeumer  P.O. Eric Mueller 《Journal of Equine Veterinary Science》2009

The objective of this study was to evaluate the efficacy of topical application of a hyaluronan (HA) derivative in wound healing with respect to the rate of epithelialization, fibroplasia, angiogenesis and contraction, magnitude of the local inflammatory response, local expression of transforming growth factor-β 1 and 3 (TGF-β 1 and 3), tumor necrosis factor-α (TNF-α), and collagen type III deposition. In six healthy adult horses, six full-thickness skin wounds were created on the dorsal aspect of both metacarpi using a sterile template. Sites were sampled at 0, 1, 2, 5, 14, 21, and 35 days following wounding. Wounds on one limb were dressed with commercially available esterified HA fleece under a nonadherent dressing. The opposite limb was covered with the nonadherent dressing alone (control). Images of the most proximal wounds were used to determine the area of total healing and the relative contributions of epithelialization and contraction to healing. At each sample time, a control and treatment biopsy were taken for histological evaluation and special stains. All samples were evaluated for degree of inflammation, fibroplasia and angiogenesis; in situ hybridization for type III collagen, TGFβ1 and 3, and immunohistochemistry for TNF-α. Mean percentages of total wound healing, epithelialization, and wound contraction were not significantly different between control and treatment groups. In treated horses, initial wound expansion was significantly decreased during the first 2 weeks. Mononuclear cell numbers, counted in the granulation tissue, increased in both control and treated limbs over the entire course of the study. However at day 35 the macrophage numbers counted in the treated horses were significantly increased compared with the control limbs (P < .05). Although not statistically significant, relative staining for type III collagen in the treated wounds was less than that of control wounds. Results of the present study do not support a benefit of an exogenous HA-derivative in the healing of distal limb wounds in horses. The shortcomings of the study design are discussed.  相似文献   

Carbonic anhydrase and osteoclasts: localization by labeled inhibitor autoradiography   总被引：7，自引：0，他引：7  

C V Gay  W J Mueller 《Science (New York, N.Y.)》1974,183(123):432-434

Autoradiography with tritiated acetazolamide indicates that osteoclasts of the hen and chick contain concentrations of carbonic anhydrase which are similar to those in pancreatic acinar cells. Grain counts of osteoblasts and osteocytes were not different from background. Thus, a sufficient quantity of carbonic anhydrase seems to be present in osteoclasts to be of physiological importance in bone resorption.  相似文献   

Permafrost soil complexity evaluated by laboratory imaging Vis-NIR spectroscopy     

Carsten W. Mueller  Markus Steffens  Henning Buddenbaum 《European Journal of Soil Science》2021,72(1):114-119

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Effects of Surface Application of MSW Compost On Cotton Production – Soil Properties,Plant Responses,And Nematode Management     

A. Khalilian  M. J. Sullivan  J.D. Mueller  A. Shiralipour  F.J. Wolak  R.E. Williamson 《Compost science & utilization》2013,21(3):270-279

Equipment was modified and/or developed for broadcast and banded applications of municipal solid waste (MSW) compost at selected rates to agricultural land for cotton production. Replicated tests were conducted for four years to determine the effects of compost on soil properties, crop yield, and nematode populations. Soil type in the test field was Faceville loamy sand. Broadcast application of compost significantly reduced soil compaction in the top 30 cm of soil in cotton rows and row-middles compared to no compost application. Banded application did not affect compaction in the row middles. Columbia lance nematode densities decreased in all compost-treated plots during all four years of study. Several plots treated with compost had nematode densities comparable to those found in the plots treated with Temik 15G nematicide. Compost application significantly increased the soil organic matter content and soil nitrogen content at six and 14 weeks after planting. However, the compost did not affect the leaf nitrogen content of the cotton plant during the same sampling periods. In 1996, 1997, and 1998, all rates of added compost significantly increased seed cotton yield. Yield increase was proportional to application rate. There were very few carry-over compost effects from each previous year's treatments on either soil organic matter, soil nitrogen, or seed cotton yield.  相似文献   

Utilization of deoiled castor cake for crop production     

AP Gupta  RS Antil  RP Narwal 《Archives of Agronomy and Soil Science》2013,59(4-5):389-395

A screen house experiment was undertaken to study effect of time of application of deoiled castor cake on the yield, N and P uptake by wheat crop. The results indicate that the wheat growth decreased significantly with increasing dose of castor cake, when castor cake was applied just before sowing of wheat. When castor cake was applied 10 days before wheat sowing, the dry matter yield (DMY) of wheat increased up to 0.125% dose of castor cake and reduced thereafter. But when the castor cake was applied 20 days before wheat sowing, the DMY was at par under all the doses of castor cake and better than control indicating the degradation of the toxicants produced by the castor cake. Application of castor cake can also be helpful in reducing the cost of phosphatic fertilizer. Thus it can be concluded that castor cake should be applied at least 3 weeks before sowing of the crop and keeping the field moist for degradation of the toxicants.  相似文献   

Implications of soil substrate and land use for properties of fen soils in North-East Germany Part I: Basic soil conditions,chemical and biological properties of topsoils     

Dr Lothar Mueller  Stephan Wirth  Elke Schulz  Axel Behrendt  Axel Hoehn  Uwe Schindler 《Archives of Agronomy and Soil Science》2013,59(2):113-126

Abstract

The objective of the paper was to analyse the implications of the origin of peat (muck) soil substrate, the current type of land use and the state of anthropogenic soil development for the topsoil properties of fens. Chemical and biological properties of peat soils of the Rhin-Havelluch lowland and the Uckermark rural landscape were analyzed. The unit water content according to Ohde and the ash content were utilized to characterize the anthropogenic development status of peat topsoils. Several chemical properties were significantly influenced by soil substrate, in particular by the proportion and kind of the mineral component. The substrate was associated with the hydrological type of mire and the soil development state. TOC/N ratio and microbial activity were increased in cases of high lime spring mires and moorshified low ash peat. The proportion of easily soluble organic carbon increased, whereas the sulphur content decreased with the soil development state. The nitrogen content and the proportions of oxalate soluble iron and aluminium reached maxima in the moorshified state. The type of land use (grassland, forest) significantly influenced the topsoil pH and the proportion of oxalate soluble phosphorus. Soils under forest were clearly determined by topsoil acidification.  相似文献   

Imaging diagnosis and clinical presentation of a Chiari malformation in a Thoroughbred foal     

A. Lempe  M. Heine  B. Bosch  K. Mueller  W. Brehm 《Equine Veterinary Education》2012,24(12):618-623

A newborn Thoroughbred foal was presented to the clinic with ambiguous neurological deficits, spinal anomalies and a soft tissue swelling dorsal to the lumbar vertebral column. The foal was alert but unable to rise and stand. With radiography, ultrasonography, computed tomography (CT) and magnetic resonance imaging (MRI) a lumbar dysraphic anomaly, cerebellar herniation and coincidental skeletal abnormalities were documented. Finally, a meningomyelocele was defined and, in context with the cerebellar herniation through the foramen magnum, the foal was diagnosed to have a Chiari malformation. The MRI examination corresponded best with the post mortem findings. Although 3‐dimensional imaging methods have been considered superior regarding full and detailed assessment of the congenital malformation, radiography and ultrasonography also provide essential information to diagnose dysraphic lesions at reduced costs and efforts. A Chiari malformation should be considered as a differential diagnosis in foals with neurological deficits.  相似文献   

Reproducibility of a semiquantitative method to assess cutaneous cytology     

SC Budach  RS Mueller 《Veterinary dermatology》2012,23(5):426-e80

Background – Cutaneous cytology is used in veterinary dermatology to assess bacteria and yeast on the skin surface and in the ears for diagnostic purposes and to monitor treatment success. A number of methods were used in reported studies to quantify micro‐organisms on cytology, but evaluation of the intra‐ and interobserver reliability of the methods is rare. Objective – The aim of this study was to evaluate the intra‐ and interobserver reliability of a semiquantitative cytology assessment method frequently used in practice. Methods – A total of 60 experienced and inexperienced veterinarians and veterinary students were asked to evaluate 10 glass slides and 18 photographs of cutaneous cytology twice. Cocci, rods, yeast, neutrophilic and eosinophilic granulocytes and macrophages were graded from 0 to 4+. Results – The intra‐observer reproducibility for evaluating the slides in the experienced group was 84.3%; in the inexperienced group it was 82.6%. For the photographs, the intra‐observer reproducibility was 92.1% in both groups. The interobserver reproducibility for evaluating the slides was 81.6 and 81.0% in the experienced and inexperienced group, respectively; corresponding values for the photographs were 91.0 and 90.0%. There was no significant difference between different participants or between the first and second evaluation by each participant for any of the parameters graded. Conclusion and clinical importance – Based on these results, this semiquantitative method of grading can be recommended for evaluating and monitoring of antimicrobial therapy in daily practice.  相似文献   

Double-blinded, placebo-controlled study to evaluate an antipruritic shampoo for dogs with allergic pruritus     

Schilling J  Mueller RS 《The Veterinary record》2012,171(4):97

Shampoo therapy is frequently used on pruritic dogs. However, there are few double-blinded, placebo-controlled studies of this form of therapy. This randomised, double-blinded, placebo-controlled study evaluated the efficacy of a commercial medicated shampoo (DermaTopic; Almapharm) containing chlorhexidine, lactoferrin, piroctone olamine, chitosan and essential fatty acids in 27 dogs with mild to moderate allergic pruritus without secondary skin infections. All dogs received shampoo therapy with either DermaTopic or a shampoo vehicle as placebo twice weekly for four weeks. The extent of pruritus was evaluated before the study and then on a daily basis by the owners using a visual analogue scale. Before beginning the treatment and after four weeks, the skin lesions were evaluated by an experienced clinician with a validated lesion score (Canine Atopic Dermatitis Extent and Severity Index - CADESI). The pruritus was reduced significantly by both DermaTopic and placebo. However, there was no significant difference between both groups. There was no statistically significant difference in the CADESI scores pre- and post-treatment in either group or between the two types of treatment. This study provides further evidence of the benefit of shampoo therapy for pruritic dogs.  相似文献   

Dermatophytes in pet Guinea pigs and rabbits     

Kraemer A  Mueller RS  Werckenthin C  Straubinger RK  Hein J 《Veterinary microbiology》2012,157(1-2):208-213

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