Molecular simulations probing Kushecarpin A as a new lipoxygenase inhibitor     

Nisar M  Kaleem WA  Khan I  Adhikari A  Khan N  Shah MR  Khan IA  Qayum M  Samiullah  Ismail M  Aman A 《Fitoterapia》2011,82(7):1008-1011

Zizyphus oxyphylla Edgew is used in Pakistan as a folk medicinal remedy for inflammatory conditions, pains especially rheumatic pain, fevers, allergy and diabetes. The aim of the current study was to scientifically validate the folk use of Z. oxyphylla Edgew by using the isolated compound in vitro and in vivo levels. Kushecarpin A was isolated from ethyl acetate fraction of the plant crude extract. Molecular docking simulations predicted Kushecarpin A as a potential new lipoxygenase (LOX) inhibitor. Kushecarpin A showed significant lipoxygenase inhibition (IC50: 7.2 ± 0.02 μM) thus validated computational prediction. It also exhibited significant and highly significant inhibition (p < 0.05 and p < 0.01) of carrageenan-induced hind paw oedema at the doses of 5, 10 and 20 mg/kg. Kushecarpin A seems to be a potentially new anti-inflammatory compound responsible for anti-inflammatory activities of Z. oxyphylla Edgew. In vitro and in vivo anti-inflammatory inflammatory activities were found in good agreement with the folk medicinal use of Z. oxyphylla Edgew in inflammatory disorders.  相似文献   

Molecular simulations of Taxawallin I inside classical taxol binding site of β-tubulin     

Khan I  Nisar M  Ahmad M  Shah H  Iqbal Z  Saeed M  Halimi SM  Kaleem WA  Qayum M  Aman A  Abdullah SM 《Fitoterapia》2011,82(2):276-281

A new taxoid Taxawallin I (1) along with two known taxoids (2-3) were isolated from methanolic bark extract of Taxus wallichiana Zucc. Structural characterization was confirmed by mass and NMR spectral techniques. Taxawallin I exhibited significant in-vitro anticancer activity against HepG2, A498, NCI-H226 and MDR 2780AD cancer lines. Tubulin binding assay was performed to assess its tubulin binding activity. Molecular docking analysis was performed to study the potential binding mode inside the taxol binding site of β-tubulin.  相似文献   

Diversity in phosphorus mobilisation and uptake in ectomycorrhizal fungi     

Claude Plassard  Julien Louche  Muhammad A. Ali  Myriam Duchemin  Elvira Legname  Benoît Cloutier-Hurteau 《Annals of Forest Science》2011,68(1):33-43

• Introduction   

Phosphorus (P) is often the first or second element limiting aboveground net primary productivity of forests. Besides low available inorganic orthophosphate (Pi) concentrations, soil may contain high total P contents, as insoluble mineral P or as organic P. Most plants form mycorrhizal associations that improve their P nutrition. Three main hypotheses have been proposed to explain this positive effect through an increase of (1) P mobilisation from mineral P, (2) P mobilisation from organic P and (3) soil exploration and P uptake. However, the positive effect of mycorrhizal symbiosis may be variable with the fungal species forming the association. This could be due to the different abilities of mycorrhizal fungi to mobilise P and/or to take up Pi from the soil.  相似文献   

Characterizing Groundwater Dynamics Based on Impact of Pulp and Paper Mill Effluent Irrigation and Climate Variability     

Shahbaz Khan  Muhammad Nadeem Asghar  Tariq Rana 《Water, air, and soil pollution》2007,182(1-4):131-148

Automated synoptic weather typing and robust orthogonal stepwise regression analysis (via principal components analysis) were applied together to develop within-weather-type air pollution prediction models for a variety of pollutants (specifically, carbon monoxide – CO, nitrogen dioxide – NO₂, ozone – O₃, sulphur dioxide – SO₂, and suspended particles – SP) for the period 1974–2000 in south-central Canada. The SAS robust regression procedure was used to limit the influence of outliers on air pollution prediction algorithms. Six-hourly Environment Canada surface observed meteorological data and 6-hourly US National Centers for Environmental Prediction (NCEP) reanalysis data of various weather elements were used in the analysis. The models were developed using two-thirds of the total years for meteorological and air pollution data; the remaining one-third (randomly selected) was used for model validation. Robust stepwise regression analysis was performed to analytically determine the meteorological variables that might be used to predict air pollution concentrations. There was a significant correlation between observed daily mean air pollution concentrations and model predictions. About 20, 50, and 80% of the 80 prediction models across the study area possessed R ² values ≥ 0.7, 0.6, and 0.5, respectively. The results of model validation were similar to those of model development, with slightly smaller model R ² values.  相似文献   

Infection and colonization of aseptically micropropagated sugarcane seedlings by nitrogen-fixing endophytic bacterium, Herbaspirillum sp. B501gfp1     

Joyce Njoloma  Kazunori Tanaka  Tasuku Shimizu  Tomohiro Nishiguchi  Muhammad Zakria  Ryo Akashi  Moriya Oota  Shoichiro Akao 《Biology and Fertility of Soils》2006,43(2):137-143

In this study, we used Herbaspirillum sp. B501gfp1 (B501gfp1), an isolate from wild rice, to investigate the interaction between a non-host nitrogen-fixing endophytic bacterium and micropropagated sugarcane plants under aseptic condition. Two Japanese sugarcane plants (Saccharum sp.) cultivars (cvs) NiF8 and Ni15 were inoculated using B501gfp1 in two inoculum doses of 10⁸ and 10² bacterial-cells-per-milliliter suspension. The results showed that bacterial cells colonized both the root and stem tissues, and colonization was apparent in the intercellular spaces. Higher bacterial numbers were detected in plant tissues inoculated with the higher inoculum concentration treatment. Bacterial numbers also varied between the two cultivars, with the higher values determined in cv Ni15. This study provides evidence that Herbaspirillum sp. B501gfp1, a rice isolate, could colonize sugarcane tissues, suggesting non-specificity of host plant among endophytes.  相似文献   

Zinc fertilization of lentil for grain yield and grain zinc concentration in ten Saskatchewan soils     

Muhammad A Maqsood  Jeff Schoenau  Albert Vandenberg 《Journal of plant nutrition》2016,39(6):866-874

Fertilization of grain legumes with zinc (Zn) can affect both marketable yield and Zn content of the grain, which is important in addressing human nutritional deficiencies in certain regions of the world. A pot experiment was conducted to determine the response of three different market classes of lentil to Zn fertilization using ten surface soils from Saskatchewan (Canada). The distribution of Zn among labile and stable fractions chemically separated from the soil was also determined in the ten prairie soils and related to the lentil responses observed. The three market classes of lentils (large and small green, small red) were grown without Zn (control), and with 2.5 and 5 kg Zn ha^?1 added as zinc sulfate to each soil prior to planting. Zinc fertilizer application significantly influenced grain yield and was soil dependent. A significant increase in grain yield over the control was observed from application of Zn on some low organic matter, high pH Brown Chernozem soils whereas a decrease in grain yield over control was observed in other soils such as a Black Chernozem of high organic matter content and low (<7) pH. Lack of positive yield response to addition of Zn were related to measured high diethylene triamine pentaacetic acid (DTPA) extractable and plant root simulator (PRS) resin membrane probe Zn, and large amounts of native Zn in exchangeable and iron/manganese (Fe/Mn) oxide bound fractions. Application of Zn fertilizer generally increased the grain concentration of Zn. For example, an increase of ～20% in Zn concentration over control was observed when 5 kg Zn ha^?1 was added to a loamy textured low organic matter Brown Chernozem soil. Overall, small green lentil was more consistent in producing a positive response to Zn fertilizer application on soils with low plant available Zn compared to large green lentil and small red lentil.  相似文献   

Boron fertilization improves seed yield and harvest index of Camelina sativa L. by affecting source-sink     

Shahbaz Khan  Hafeez ur Rehman  Muhammad Ashfaq Wahid  Muhammad Farrukh Saleem  Mumtaz Akhtar Cheema  Shahzad Maqsood Ahmed Basra 《Journal of plant nutrition》2016,39(12):1681-1687

The impact of soil (1, 2 kg ha^?1) and foliar (100, 200 mg L^?1) boron (B) with control (no B) was evaluated on phenology and yield formation of Camelina each applied at stem elongation and flowering stages. Foliar (200 mg L^?1) or soil B (2 kg ha^?1) resulted in earlier flowering and maturity, increased fruit bearing branches (19.68%), number of siliqua, seeds per siliqua (4.6%), biological yield (15%), seed yield (24%), harvest index (11.4%) and oil contents (23%) than no B. Increased fruit bearing branches, seed filled siliqua or seed numbers, harvest index and oil quality can be attributed to changes in dry matter accumulated of stem with simultaneous increase in siliqua dry weight with foliar or soil applied B. In crux, foliar (200 mg L^?1) or soil applied (2 kg ha^?1) B seems promising to improve seed and oil yield, harvest index of Camelina sativa under B deficient condition.  相似文献   

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A     

Cruz-Migoni A  Hautbergue GM  Artymiuk PJ  Baker PJ  Bokori-Brown M  Chang CT  Dickman MJ  Essex-Lopresti A  Harding SV  Mahadi NM  Marshall LE  Mobbs GW  Mohamed R  Nathan S  Ngugi SA  Ong C  Ooi WF  Partridge LJ  Phillips HL  Raih MF  Ruzheinikov S  Sarkar-Tyson M  Sedelnikova SE  Smither SJ  Tan P  Titball RW  Wilson SA  Rice DW 《Science (New York, N.Y.)》2011,334(6057):821-824

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.  相似文献   

Comparison of biological parameters between the invasive B biotype and a new defined Cv biotype of <Emphasis Type="Italic">Bemisia tabaci</Emphasis> (Hemiptera: Aleyradidae) in China     

Bao-Li Qiu  Fang Dang  Shao-Jian Li  Muhammad Z. Ahmed  Feng-Liang Jin  Shun-Xiang Ren  Andrew G. S. Cuthbertson 《Journal of pest science》2011,84(4):419-427

The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a widely distributed and destructive agricultural pest on various host plants. The biology of two biotypes of B. tabaci: the invasive B and a new defined Cv biotype, on a range of host plants (hibiscus, laurel, poinsettia, collard, cucumber and tomato) were studied in the laboratory. Results revealed that the developmental periods of the B biotype immatures were not significantly different on the tested host plants except those between laurel and collard. The Cv biotype immatures developed significantly slower on cucumber and tomato than on the other plants. B. tabaci B biotype had the highest survivorship on collard (68.55%), and the lowest on laurel (33.24%), while the Cv biotype had the highest and lowest survivorships on laurel (61.63%) and tomato (36.74%). Host plants did not significantly affect the pre-ovipostion period regardless of biotype. The longest averaged longevity and highest fecundity of B biotype were both recorded on collard: 25.15 days and 143.0 eggs. The highest fecundity of Cv biotype was 196.49 eggs on laurel and its longest longevity was on hibiscus (19.62 days). The intrinsic rate of natural increase (r _m) of B biotype on the three vegetables were all higher than those on the three ornamentals whereas the r _m of Cv biotype on the three ornamentals were all higher that those on the three vegetables. Our research indicates that B. tabaci B and Cv biotypes have different host plant suitabilities. The three tested vegetables were more suitable for B biotype while the three tested ornamental plants were more suitable for Cv biotype. The potential mechanism for the different suitability of B and Cv biotypes on various host plants is also discussed.  相似文献   

Preference and performance of <Emphasis Type="Italic">Plutella xylostella</Emphasis> (L.) (Lepidoptera: Plutellidae) on canola cultivars     

Seyed Ali Asghar Fathi  Maryam Bozorg-Amirkalaee  Rana Muhammad Sarfaraz 《Journal of pest science》2011,84(1):41-47

The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is an important insect pest of canola, Brassica napus L., in Ardabil, Iran. Host plant resistance is an essential component of integrated management of P. xylostella. We investigated the preferences and performance of P. xylostella on nine commercial cultivars of canola, namely Zarfam, RGS003, Adder, Okapi, Opera, Hyola401, Ebonite Option500 and Elite under greenhouse conditions at 23 ± 1°C, 50 ± 5% RH and 14L:10D. In free-choice situation, oviposition was lowest on Opera (23.5) and highest on Zarfam (44.7). In the life table study, fecundity of new generation female moths was lowest on Opera (95.4) and highest on Adder (145.7). Survival from egg to adult was significantly lower on Opera, Option500 and Hyola401 than on other tested cultivars. The intrinsic rate of natural increase (r _m) and population growth rate (λ) were lowest on Opera and highest on Zarfam. The generation time (T) was shortest on Zarfam (17.2 days) and longest on Hyola401 (19.9 days) whereas doubling time (DT) was longest on Opera (3.9 days) and shortest on Zarfam (3.0 days). Our results clearly suggest that Opera was the most resistant host among the tested cultivars and has the potential to be used in the integrated management of P. xylostella.  相似文献