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21.
Murali Mohan Sabbavarapu Mamta Sharma Siva Kumar Chamarthi Nayakoti Swapna Abhishek Rathore Mahendar Thudi Pooran Mal Gaur Suresh Pande Sarvjeet Singh Livinder Kaur Rajeev Kumar Varshney 《Euphytica》2013,193(1):121-133
Fusarium wilt (FW) and Ascochyta blight (AB) are two important diseases of chickpea which cause 100 % yield losses under favorable conditions. With an objective to validate and/or to identify novel quantitative trait loci (QTLs) for resistance to race 1 of FW caused by Fusarium oxysporum f. sp. ciceris and AB caused by Ascochyta rabiei in chickpea, two new mapping populations (F2:3) namely ‘C 214’ (FW susceptible) × ‘WR 315’ (FW resistant) and ‘C 214’ (AB susceptible) × ‘ILC 3279’ (AB resistant) were developed. After screening 371 SSR markers on parental lines and genotyping the mapping populations with polymorphic markers, two new genetic maps comprising 57 (C 214 × WR 315) and 58 (C 214 × ILC 3279) loci were developed. Analysis of genotyping data together with phenotyping data collected on mapping population for resistance to FW in field conditions identified two novel QTLs which explained 10.4–18.8 % of phenotypic variation. Similarly, analysis of phenotyping data for resistance to seedling resistance and adult plant resistance for AB under controlled and field conditions together with genotyping data identified a total of 6 QTLs explaining up to 31.9 % of phenotypic variation. One major QTL, explaining 31.9 % phenotypic variation for AB resistance was identified in both field and controlled conditions and was also reported from different resistant lines in many earlier studies. This major QTL for AB resistance and two novel QTLs identified for FW resistance are the most promising QTLs for molecular breeding separately or pyramiding for resistance to FW and AB for chickpea improvement. 相似文献
22.
Murali K. Darapuneni Gaylon D. Morgan Amir M. H. Ibrahim Robert W. Duncan 《Euphytica》2014,195(2):279-285
Vernalization and photoperiodism are two important physiological processes related to yield of many cool-season annual crops. The flowering response of 20 flax (Linum usitatissimum L.) genotypes to two vernalization regimes (vernalized and unvernalized) and two photoperiod treatments (10 and 14 h) was evaluated in a growth chamber study in 2010 and 2011. The results suggest that photoperiod, vernalization, and genotype all had an effect on earliness as measured by days to anthesis. Unlike flax grown in the Upper US Midwest and Canada, Texas flax is grown in the fall due to high spring and summer temperatures. Genotype interaction was observed with both vernalization and photoperiod. Specifically, flax genotypes from Texas (winter type) were sensitive to both vernalization and photoperiods for flowering. Texas genotypes delayed anthesis for 7 days or more in unvernalized seedlings, whereas flowering time of most other spring grown flax genotypes was unaffected by the vernalization treatments. Texas genotypes also delayed anthesis for 12 days or more under vernalized and short day conditions, whereas most other genotypes were not influenced by photoperiodism in vernalized seedlings. The selection for vernalization and photoperiodic sensitivity in Texas genotypes and introgression of these traits into recently adapted spring grown genotypes is needed for development of high yielding flax genotypes for southern Great Plains production areas. 相似文献
23.
Xenobiotic-metabolizing enzymes in canine mammary tumours 总被引：1，自引：0，他引：1
Kumaraguruparan R Subapriya R Balachandran C Manohar BM Thangadurai A Nagini S 《Veterinary journal (London, England : 1997)》2006,172(2):364-368
Mammary tumours are the most common neoplasms in female dogs. The present study was designed to evaluate the relationship between different clinical stages with activities of phase I and phase II carcinogen-metabolizing enzymes in canine mammary tumours. The levels of cytochrome P450 and cytochrome b5 and the activities of glutathione S-transferase (GST), gamma-glutamyl transpeptidase (GGT), DT-diaphorase (DTD) and NADPH diaphorase in tumour tissues of 25 bitches was estimated. Enhanced levels of cytochrome P450 and b5 and phase II enzyme activities were observed in tumour tissues compared to the corresponding uninvolved adjacent tissues. The magnitude of the changes in phase I and phase II enzyme status was, however, more pronounced in stages I and II compared to stages III and IV. The results suggest that the balance between phase I carcinogen activation and phase II detoxification systems may play an important role in canine mammary tumour development. 相似文献
24.
The present study was conducted to assess the haemagglutination-inhibition (HI) titres required to protect the chicken reproductive
tract against direct damage caused by Newcastle disease virus (NDV). Precociously induced oviduct and uterus by oestrogen
treatment of young chicks were used to assess the damage or protection against the damage by analysis of ciliostasis or histopathological
lesions. Unvaccinated day-old female white leghorn chickens were used as the maternally derived antibody (MDA) group. Chickens
were vaccinated with either a live lentogenic vaccine on day 14 of age or, along with it, an inactivated vaccine at day 36
of age, to generate birds with a range of primary or secondary response induced HI antibodies. Birds with different HI antibody
levels were challenged with virulent NDV. It was found that a HI antibody titre of 128 and above was protective against direct
damage of the reproductive tract, while the 32–64 titre range was protective when derived through secondary vaccination only. 相似文献
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26.
The aim of this paper is to analyze the level of dependency of rural households on forests, based on the economic status of the households and the property regimes of forests. The analysis of household-level dependency suggests that large numbers of rural households are benefiting regularly from their community forests. Out of three forest property regimes examined, community forests were found to be the most prominent sources for supplying forest products to local households. Analysis of the degree of forest-products dependency and the economic status of households shows that the low economic status households rely more on the community forest than the economically better-off households. In addition, a 2 analysis indicates that the economic status of households affects the degree of forest-product collection activities. The results show three types of property regime with respect to forest management in Chitwan. Each regime holds its own ownership rights, which control the harvesting of forest products and provides incentives to the local households. 相似文献
27.
S. Murali K. Sathish Kumar P. V. Alfiya D. S. Aniesrani Delfiya Manoj P. Samuel 《Journal Of Aquatic Food Product Technology》2019,28(5):541-554
The aim of the study was to investigate the drying kinetics and quality characteristics of Indian mackerel dried under solar–electrical hybrid dryer (S-EHD). Fresh Indian mackerel fishes (Rastrelliger kanagurta) were cleaned, cut into butterfly fillets, and salted overnight using a dry salting method (salt-to-fish ratio, 1:3). The salted mackerel was dried in a S-EHD at the air temperature of 45–55°C, relative humidity of 47–62%, and air velocity of 0.60–0.80 m/s. Open sun drying (OSD) of salted Indian mackerel was conducted to compare with S-EHD. The moisture content of the salted mackerel (61.5% w.b.) was reduced to 31.8% (w.b.) under S-EHD and 30.25% (w.b) under OSD in 8 and 32 h, respectively. The drying rate curve showed that mackerel drying occurred under falling-rate drying period in both the drying methods. A drying efficiency of 23.81% was observed for salted Indian mackerel drying under S-EHD. Diffusion approach and two-term models were selected to accurately predict the drying behavior of mackerel under S-EHD and OSD, respectively. Total volatile base nitrogen (TVB-N), trimethylamine, and thiobarbituric acid analysis of dried samples revealed that the mackerel dried under S-EHD was better than OSD. In the sensory analysis, samples dried under S-EHD recorded highest overall acceptability score. 相似文献
28.
Y-S Lai H-C Chiu S Murali I-C Guo S-C Chen K Fang & C-Y Chang 《Journal of fish diseases》2001,24(4):237-244
Mouse monoclonal antibodies (MAbs) were produced by using yellow grouper nervous necrosis virus (YGNNV) as an immunogen, isolated from infected yellow grouper, Epinephelus awoara (Temminck & Schlegel), and propagated in GB cells. In enzyme linked immunosorbent assay (ELISA), 43 hybridoma clones secreting MAbs strongly reacted with the purified virus. Ten of them showed a higher neutralization index (NI) value between 6.5 and 4.5 (log10 NI) than the other 33 MAbs against YGNNV infection in cell culture. All 10 MAbs belonged to the IgG isotype with a κ light chain and recognized the 42 kDa coat protein of YGNNV by Western blot analysis. Immunohistochemical results demonstrated that the viral signals co-located with pathological lesions observed in retina, brain and spinal cord. These results indicate that the MAbs are useful for confirmative diagnosis of YGNNV infection. 相似文献
29.
White spot disease (WSD) is an important viral disease of penaeid shrimp caused by white spot syndrome virus (WSSV). WSSV isolated from WSD outbreaks in commercial shrimp (Penaeus monodon) farms in India were propagated in the laboratory in healthy shrimp. The virus was purified from the infected tissues by sucrose gradient centrifugation. The VP28 was electroeluted from SDS-PAGE gels and was used to immunize Balb/c mice to produce hybridomas secreting monoclonal antibodies (MAb) against WSSV. A total of five hybridoma clones secreting MAbs to VP28 were produced. The MAbs were of the isotypes IgG1, IgG2b and IgM. The MAbs reacted with VP28 of WSSV and not with any other viral or shrimp protein in western blot. The MAbs were used to develop dot immunoblot assay using an immunocomb to detect WSSV from field samples. The test developed had an analytical sensitivity of 625 pg and a diagnostic sensitivity of 100% compared to single step polymerase chain reaction (PCR). The test can be used as an alternate for first step PCR to detect WSSV from field samples. 相似文献
30.
James C. Russell Ephraim M. Hanks Murali Haran 《Journal of Agricultural, Biological & Environmental Statistics》2016,21(1):22-40
When analyzing animal movement, it is important to account for interactions between individuals. However, statistical models for incorporating interaction behavior in movement models are limited. We propose an approach that models dependent movement by augmenting a dynamic marginal movement model with a spatial point process interaction function within a weighted distribution framework. The approach is flexible, as marginal movement behavior and interaction behavior can be modeled independently. Inference for model parameters is complicated by intractable normalizing constants. We develop a double Metropolis–Hastings algorithm to perform Bayesian inference. We illustrate our approach through the analysis of movement tracks of guppies (Poecilia reticulata). 相似文献