Canine low‐grade intra‐orbital myxosarcoma: case report          下载免费PDF全文

Cecília B. Campos  Fernanda C. Nunes  Conrado O. Gamba  Karine A. Damasceno  Cristina M. Souza  Liliane C. Campos  Geovanni D. Cassali 《Veterinary ophthalmology》2015,18(3):251-253

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Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues     

Marion Papin  Cyrille Guimaraes  Benoit Pierre-Aue  Delphine Fontaine  Jeoffrey Pardessus  Hlne Couthon  Gaëlle Fromont  Karine Maho  Aurlie Chantme  Christophe Vandier  Michelle Pinault 《Marine drugs》2022,20(4)

Ether lipids are composed of alkyl lipids with an ether bond at the sn-1 position of a glycerol backbone and alkenyl lipids, which possess a vinyl ether bond at the sn-1 position of the glycerol. These ether glycerolipids are present either as polar glycerophospholipids or neutral glycerolipids. Before studying the biological role of molecular species of ether glycerolipids, there is a need to separate and quantify total alkyl and alkenyl glycerolipids from biological samples in order to determine any variation depending on tissue or physiopathological conditions. Here, we detail the development of the first high-performance thin-layer chromatography method for the quantification of total alkyl and alkenyl glycerolipids thanks to the separation of their corresponding alkyl and alkenyl glycerols. This method starts with a reduction of all lipids after extraction, resulting in the reduction of neutral and polar ether glycerolipids into alkyl and alkenyl glycerols, followed by an appropriate purification and, finally, the linearly ascending development of alkyl and alkenyl glycerols on high-performance thin-layer chromatography plates, staining, carbonization and densitometric analysis. Calibration curves were obtained with commercial alkyl and alkenyl glycerol standards, enabling the quantification of alkyl and alkenyl glycerols in samples and thus directly obtaining the quantity of alkyl and alkenyl lipids present in the samples. Interestingly, we found a differential quantity of these lipids in shark liver oil compared to chimera. We quantified alkyl and alkenyl glycerolipids in periprostatic adipose tissues from human prostate cancer and showed the feasibility of this method in other biological matrices (muscle, tumor).  相似文献   

Survival of Curtobacterium flaccumfaciens pv. flaccumfaciens in weeds     

Daniele M. Nascimento  Letícia R. Oliveira  Luana L. Melo  João C. Silva  José M. Soman  Karine T. Girotto  Renan P. Eburneo  Marcos R. Ribeiro-Junior  Maria M. P. Sartori  Tadeu A. F. Silva Júnior  Antonio C. Maringoni 《Plant pathology》2020,69(7):1357-1367

Weeds are important alternative hosts of pathogens, responsible for the survival and spread of phytopathogenic bacteria. Our study evaluated the potential of weeds as hosts of Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), causal agent of bacterial wilt, one of the main diseases of common beans. Cff survival was evaluated in the phyllosphere and in the rhizosphere of 21 weeds, in four experiments under field conditions, during the years 2018 and 2019. The aerial part of the plant was inoculated by spraying bacterial suspension (10⁷ cfu/ml) of Cff, while the soil of the growing pots was infested with the same suspension. Cff survival was evaluated every 7 days, for 70 days. The identity of the bacterium was confirmed by PCR with the specific primers CffFOR2 and CffREV4, from strains recovered from all samples. Principal component analysis (PCA) showed that high temperatures and rainfall reduced Cff survival in the phyllosphere, while high temperatures reduced the survival of the bacterium in the rhizosphere. Our results demonstrated that Amaranthus viridis (family Amaranthaceae), Conyza bonariensis, Emilia fosbergii, Galinsoga parviflora, Gnaphalium purpureum (Asteraceae), Raphanus sativus, Lepidium virginicum (Brassicaceae), Commelina benghalensis (Commelinaceae), Ipomoea triloba (Convolvulaceae), Cyperus rotundus (Cyperaceae), Senna obtusifolia (Fabaceae), Digitaria insularis (Poaceae), Nicandra physalodes, and Solanum americanum (Solanaceae) are potential hosts for Cff. Their eradication in common bean fields is recommended, especially in fields with a history of bacterial wilt occurrence.  相似文献   

The effects of a loading dose followed by constant rate infusion of xylazine compared with romifidine on sedation,ataxia and response to stimuli in horses     

Simone K Ringer  Karine Portier  Paul R Torgerson  Rachel Castagno  Regula Bettschart‐Wolfensberger 《Veterinary anaesthesia and analgesia》2013,40(2):157-165

ObjectiveTo compare xylazine and romifidine constant rate infusion (CRI) protocols regarding degree of sedation, and effects on postural instability (PI), ataxia during motion (A) and reaction to different stimuli.Study designBlinded randomized experimental cross-over study.AnimalsTen adult horses.MethodsDegree of sedation was assessed by head height above ground (HHAG). Effects on PI, A and reaction to visual, tactile and acoustic stimulation were assessed by numerical rating scale (NRS) and by visual analogue scale (VAS). After baseline measurements, horses were sedated by intravenous loading doses of xylazine (1 mg kg^?1) or romifidine (80 μg kg^?1) administered over 3 minutes, immediately followed by a CRI of xylazine (0.69 mg kg^?1 hour^?1) or romifidine (30 μg kg^?1 hour^?1) which was administered for 120 minutes. Degree of sedation, PI, A and reaction to the different stimuli were measured at different time points before, during and for one hour after discontinuing drug administration. Data were analysed using two-way repeated measures anova, a Generalized Linear Model and a Wilcoxon Signed Rank Test (p < 0.05).ResultsSignificant changes over time were seen for all variables. With xylazine HHAG was significantly lower 10 minutes after the loading dose, and higher at 150 and 180 minutes (i.e. after CRI cessation) compared to romifidine. Reaction to acoustic stimulation was significantly more pronounced with xylazine. Reaction to visual stimulation was greater with xylazine at 145 and 175 minutes. PI was consistently but not significantly greater with xylazine during the first 30 minutes. Reaction to touch and A did not differ between treatments. Compared to romifidine, horses were more responsive to metallic noise with xylazine.ConclusionsTime to maximal sedation and to recovery were longer with romifidine than with xylazine.Clinical relevanceWith romifidine sufficient time should be allowed for complete sedation before manipulation.  相似文献   

E-commerce development in rural and remote areas of BRICS countries          下载免费PDF全文

Karine HAJI 《农业科学学报》2021,20(4):979-997

E-commerce plays an essential role in modern trade today. It is expected that e-commerce volume amounted to 29 trillion USD in the world in 2017, and would grow with the spread of the Internet and information and communication technologies(ICTs). Brazil, Russia, India, China and South Africa(BRICS), together with many others, consider e-commerce a means to facilitate rapid, inclusive and sustainable economic growth, improving the living standards and alleviating poverty. This article examines areas for potential cooperation by BRICS countries in e-commerce development across rural and remote areas to fight poverty. It analyses the current state of e-commerce development in rural and remote areas in each of the BRICS countries, including cases of public and private initiatives to support it. The article also defines the opportunities which e-commerce brings to people living in rural and remote areas. Moreover, it evaluates the existing challenges and risks. The article concludes that despite the rapid e-commerce development in BRICS countries, and significant opportunities created, there are still issues of disproportionate e-commerce in varied regions and the lack of BRICS cooperation in this sphere. Based on a comparative and normative in-depth, systematic analysis, the article develops a set of recommendations for deepening BRICS countries' cooperation in the following areas: infrastructure in rural and remote regions; education; consumer protection; online dispute resolution; coordinated policy in the international scene, including representation of BRICS countries in international indexes, such as the Organization of Economic Co-operation and Development(OECD) Digital Services Trade Restrictiveness Index(STRI).  相似文献   

Comparison of transvaginal natural orifice transluminal endoscopic surgery (NOTES®) and laparoscopy for elective bilateral ovariectomy in standing mares     

Pader K  Freeman LJ  Constable PD  Wu CC  Snyder PW  Lescun TB 《Veterinary surgery : VS》2011,40(8):998-1008

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Nicotinic acetylcholine receptor β2 subunits in the medial prefrontal cortex control attention     

Guillem K  Bloem B  Poorthuis RB  Loos M  Smit AB  Maskos U  Spijker S  Mansvelder HD 《Science (New York, N.Y.)》2011,333(6044):888-891

More than one-third of all people are estimated to experience mild to severe cognitive impairment as they age. Acetylcholine (ACh) levels in the brain diminish with aging, and nicotinic ACh receptor (nAChR) stimulation is known to enhance cognitive performance. The prefrontal cortex (PFC) is involved in a range of cognitive functions and is thought to mediate attentional focus. We found that mice carrying nAChR β2-subunit deletions have impaired attention performance. Efficient lentiviral vector-mediated reexpression of functional β2-subunit-containing nAChRs in PFC neurons of the prelimbic area (PrL) completely restored the attentional deficit but did not affect impulsive and motivational behavior. Our findings show that β2-subunit expression in the PrL PFC is sufficient for endogenous nAChR-mediated cholinergic regulation of attentional performance.  相似文献   

Successful treatment of an intrathoracic bronchogenic cyst in a Holstein-Friesian calf     

Beat Berchtold  Mireille Meylan  Karine Gendron  Ute Morath  Ulrich Rytz  Beatrice Lejeune 《Acta veterinaria Scandinavica》2013,55(1):14

A 5-½-month-old female Holstein-Friesian calf was presented with a history of recurring ruminal tympany and poor development. The absence of lung sounds on the right hemithorax suggested a right-sided intrathoracic pathology. Radiography and computed tomography revealed a large thin-walled cavernous lesion with a gas-fluid interface which almost completely filled the right thoracic cavity. Fluid aspirated from the lesion was clear, yellowish and odorless. These findings led to the diagnosis of a bronchogenic cyst. Thoracotomy was performed under general anesthesia. The cyst strongly adhered to the adjacent lung tissue. After removal of the free wall, the adjacent lung tissue was sealed using surgical stapling instruments, and the non-removable part of the wall was curetted and rinsed. The intensive postoperative management included antibiotic therapy, oxygen supplementation and regional lidocaine infusion. Anti-inflammatory drugs were administered for further pain control. The calf recovered well and was released from the clinic on postoperative day 11. Intra- or extrathoracic bronchogenic cysts result from abnormal budding during the embryonic development of the tracheobronchial system. Successful treatment of this calf despite the size of the lesion and the invasive character of the surgical intervention indicates that resection of bronchogenic cysts in cattle may be an option for valuable animals.  相似文献   

Isolation of Nicoletella semolina from Equine Tracheal Washes     

Karine Maillard  Eric A. Richard  Peter Kuhnert  Guillaume Fortier  Albertine Léon  Pierre-Hugues Pitel 《Journal of Equine Veterinary Science》2013

The aim of the present study was to describe the prevalence of Nicoletella semolina in equine airways and its relationships with cytological evaluation of tracheal wash (TW). Samples were collected in the framework of routine bacteriological diagnostics of equine TW between May 2010 and June 2011. N semolina has been isolated, along with either common pathogens or contaminants, from 19 (1.8%) of the 1,054 TW samples. Median TW neutrophil counts (87.0%) in specimens from N semolina-positive horses were significantly different from those from N semolina-“negative” horses (52.0%). The data presented in this report may suggest considering such bacteria in horses clinically suffering from airway inflammation.  相似文献   

Castration of horses under total intravenous anaesthesia: analgesic effects of lidocaine     

Karine G Portier DVM  CertVA  MSc  PhD  DVSc  Laetitia Jaillardon DVM  Elizabeth A Leece BVSc  CertVA  Diplomate ECVAA  Catherine M Walsh BSc  BVSc  DVA  Diplomate ECVAA 《Veterinary anaesthesia and analgesia》2009,36(2):173-179

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