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991.
992.
Campylobacter-like organisms isolated from gastric mucosa of ferrets 总被引:19,自引:0,他引:19
J G Fox B M Edrise E B Cabot C Beaucage J C Murphy K S Prostak 《American journal of veterinary research》1986,47(2):236-239
Campylobacter-like organisms (CLO) were isolated from gastric lesions in 1 ferret and gastric mucosa of 2 healthy ferrets. The organism was not isolated from biopsies of gastric mucosa of 14 other healthy ferrets, 1 of which had small gastric lesions located at the pylorus. Lesions from which CLO were isolated were located in the antrum of 1 ferret and were classified as inflammation with repair. Affected gastric tissue was highly vascularized with fibrous connective tissue surrounding irregularly shaped glands. Necrosis and ulceration of adjacent mucosa also were observed. Using Warthin-Starry stain, Campylobacter-like organisms were seen on and in the glandular epithelium of the ferret with gastric lesions from which CLO were isolated. 相似文献
993.
Virus-neutralizing and passively protective monoclonal antibodies to infectious bursal disease virus of chickens. 总被引:12,自引:0,他引:12
Murine monoclonal antibodies (MAbs) were produced to assist in the identification and characterization of the virus-neutralizing epitopes of infectious bursal disease virus (IBDV). Only MAbs that reacted in Western blotting with viral protein 2 (VP2) or immunoprecipitated VP2 neutralized the infectivity of the virus in cell culture and passively protected young chickens from infection. Three of the neutralizing MAbs did not react with denatured viral proteins. Additivity enzyme-linked immunosorbent assays indicated that the six virus-neutralizing MAbs recognized two spatially independent epitopes. The ability of two of the virus-neutralizing MAbs to neutralize a variant of IBDV that had escaped neutralization by all the other MAbs confirmed the existence of two distinct neutralizing epitopes. The results support the hypothesis that there are at least two non-overlapping epitopes recognized by the virus-neutralizing MAbs reported in this study, although these may still be within one conformational site on VP2 of IBDV. 相似文献
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996.
1. Bird movement was studied at various ages in two flocks of 18,200 broiler chickens. 2. One-d-old chicks placed in corners of a 29 x 14 m brooding area dispersed evenly over the whole area in a period of 48 h. 3. At 15 d of age, when released from the brooding area, birds which were among the first to move to the far end of the house did not necessarily remain in that area of the house. 4. Starting at 4 and 5 weeks of age, birds marked individually were variable in their movement throughout the house during a 16- and 9-d period respectively. Even the least mobile individual covered an area of the house containing several thousand birds. 5. Closer scrutiny of movement by individual birds showed that some moved over areas of 20 m2 in an hour. Birds moved further than was necessary simply to reach food and water. No agonistic interactions were observed and there was no evidence that crowding was a physical restraint on bird movement. 6. Results indicated that birds were not generally attached to a particular site in the shed and that social factors did not restrict movement. Some implications of bird movement are discussed with regard to performance, welfare and leg abnormalities. 相似文献
997.
C J Murphy J P Lavoie J Groff D Hacker P Pryor R W Bellhorn 《Journal of the American Veterinary Medical Association》1989,194(7):939-942
Bilateral swelling of upper and lower eyelids was caused by lymphocytic infiltration in an 8-year-old Thoroughbred mare. The condition worsened with pregnancy and became associated with subcutaneous dissemination of lymphosarcoma at distant sites. 相似文献
998.
J M Murphy G A Severin J D Lavach D I Hepler D C Lueker 《Journal of the American Veterinary Medical Association》1979,174(3):269-272
A modified Bacillus Calmette-Guerin (BCG) preparation was used successfully in the treatment of 7 cases of equine sarcoid. The BCG preparation was injected into the lesions. The longest period of remission has been 24 months, and the shortest period of remission has been 9 months, with no signs of recurrence of the tumor in any of the presented cases. 相似文献
999.
The effects of androgens and gonadotropins on testicular development in the prepubertal rat. 下载免费PDF全文
Treatment of male rat pups from five to 34 days of age with dihydrotestosterone or 5 alpha-androstane-3 alpha, 17 beta-diol, resulted in reduced testicular size at 35 days of age. This appeared to be due to decreased tubular diameters and reduced spermatocyte numbers, especially late pachytene cells in stages X to XIII. In rat pups treated with dihydrotestosterone or 5 alpha-androstane-3 alpha, 17 beta-diol, treatment with luteinizing hormone resulted in some restoration of tubular diameter and spermatocyte numbers. In 5 alpha-androstane-3 alpha, 17 beta-diol treated rat pups partial restoration of testes size resulted from follicle-stimulating hormone treatment, but tubular diameter and spermatocyte numbers were depressed. Single serum samples collected at 35 days of age were analysed for luteinizing and follicle-stimulating hormone concentrations. These data indicated that the effects of administered androgens were due primarily to depression of circulating gonadotropin concentrations but a direct inhibition at the level of the testis could not be ruled out. 相似文献
1000.
A C Ray M J Murphy M D DuVall J C Reagor 《American journal of veterinary research》1989,50(4):546-550
A method to determine residue concentrations of anti-coagulant rodenticides, brodifacoum (BF) and bromadiolone (BD) in liver was developed, using gas chromatography/mass spectrometry. Nine dogs were given 1.1 mg of BF/kg of body weight, PO, in polyethylene glycol 400, one time. Rats were fed BF or BD (via commercial baits) in amounts from 0.28 to 11.25 mg/kg over 1- to 4-day periods. Fresh liver samples were collected at necropsy from all rats and 3 dogs, ground with Na2SO4, and extracted with CHCl3:MeOH (9:1). After evaporation and silica cartridge purification were performed, residues were oxidized with a 0.16M chromic acid solution, and an oxidation product (4-bromobenzoic acid) was partitioned into CHCl3. The methylated derivative (port derivatization with trimethylanilinium hydroxide) was assayed, using gas chromatography/mass spectrometry. Bromadiolone was detected in livers from rats given greater than 6 mg of BD/kg of body weight, but not in livers of rats given 1.25 mg of BD/kg. In contrast, BF was detected (with one exception) in livers from dogs (given 1.1 mg of BF/kg) and from rats given high (11.25 mg of BF/kg) and low (0.28 mg of BF/kg) doses. This protocol, which does not differentiate between BF and BD because of the formation of a common product after chromic acid oxidation, was used to diagnose anticoagulant toxicosis in 3 dogs, 1 human being and 1 llama naturally poisoned. 相似文献