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An efficient method of repetitive somatic embryogenesis and plant regeneration was established in Coriandrum sativum L. Embryogenic callus was induced from cotyledon and hypocotyl segments on Murashige and Skoog (MS) medium with 4.52 μM 2,4-dichlorophenoxy acetic acid (2,4-D), upon subculturing on medium having same level of 2,4-D at an interval of 3 weeks developed somatic embryos, which progressed to cotyledonary stage through early developmental stages of somatic embryogenesis. The transfer of somatic embryos at an early cotyledonary and cotyledonary stage in clumps in succession to fresh 4.52 μM 2,4-D supplemented medium developed embryos in a cyclic manner. Upon transferal to embryogenic clumps (cotyledonary embryos) to modified MS medium (4 g l−1 KNO3, 0.29 g l−1 NH4NO3, 3 mg l−1 thiamine HCl, 0.5 mg l−1 pyridoxine HCl, and 5 mg l−1 nicotinic acid), the embryos irrespective of the cycles underwent maturation and germination. Germinating embryos transferred to half-strength MS medium favored healthy growth of plantlets. The system of recurrent somatic embryogenesis in coriander offers a system for genes transfer and also scale-up production of modified plants. 相似文献
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Branches of nine-year-old loblolly pine trees grown in a 2 x 2 factorial combination of fertilization and irrigation were exposed for 11 months to ambient, ambient + 175, or ambient + 350 micro mol mol(-1) CO(2). Rates of light-saturated net photosynthesis (A(max)), maximum stomatal conductance to water vapor (g(max)), and foliar nitrogen concentration (% dry mass) were assessed monthly from April 1993 until September 1993 on 1992 foliage (one-year-old) and from July 1993 to March 1994 on 1993 foliage (current-year). Rates of A(max) of foliage in the ambient + 175 CO(2) treatment and ambient + 350 were 32-47 and 83-91% greater, respectively, than that of foliage in the ambient CO(2) treatment. There was a statistically significant interaction between CO(2) treatment and fertilization or irrigation treatment on A(max) on only one measurement date for each age class of foliage. Light-saturated stomatal conductance to water vapor (g(max)) was significantly affected by CO(2) treatment on only four measurement dates. Light-saturated g(max) in winter was only 42% of summer g(max) even though soil water during winter was near field capacity and evaporative demand was low. Fertilization increased foliar N concentration by 30% over the study period when averaged across CO(2) treatments. During the study period, the ambient + 350 CO(2) treatment decreased average foliar N concentration of one-year-old foliage in the control, irrigated, fertilized and irrigated + fertilized plots by 5, 6.4, 9.6 and 11%, respectively, compared with one-year-old foliage in the corresponding ambient CO(2) treatments. The percent increase in A(max) due to CO(2) enrichment was similar in all irrigation and fertilization treatments and the effect persisted throughout the 11-month study period for both one-year-old and current-year foliage. 相似文献
25.
We investigated diurnal and seasonal changes in carbon acquisition and partitioning of recently assimilated carbon in fast- and slow-growing families of loblolly pine (Pinus taeda L.) to determine whether fast-growing families exhibited greater carbon gain at the leaf level. Since planting on a xeric infertile site in Scotland County, NC, USA in 1993, five Atlantic Coastal Plain (ACP) and five "Lost Pines" Texas (TX) families have been grown with either optimal nutrition or without fertilization (control). In 1998 and 1999, gas exchange parameters were monitored bimonthly in four families and needles were analyzed bimonthly for starch and soluble sugar concentrations. Although diurnal and seasonal effects on net photosynthesis (A(net)) and maximum rate of light-saturated photosynthesis (A(max)) were significant, few family or treatment differences in gas exchange characteristics were observed. The A(net) peaked at different times during the day over the season, and A(max) was generally highest in May. Instantaneous water-use efficiency (WUE(i)), derived from gas exchange parameters, did not differ among families, whereas foliage stable isotope composition (delta(13)C) values suggested that TX families exhibited lower WUE than more mesic ACP families. Although there were no diurnal effects on foliar starch concentrations, needles exhibited pronounced seasonal changes in absolute concentrations of total nonstructural carbohydrates (TNC), starch and soluble sugars, and in partitioning of TNC to starch and sugars, mirroring seasonal changes in photosynthesis and shoot and root growth. In all families, foliar starch concentrations peaked in May and decreased to a minimum in winter, whereas reducing sugar concentrations were highest in winter. Some family and treatment differences in partitioning of recently assimilated carbon in needles were observed, with the two TX families exhibiting higher concentrations of TNC and starch and enhanced starch partitioning compared with the ACP families. We conclude that growth differences among the four families are not a function of differences in carbon acquisition or partitioning at the leaf level. 相似文献
26.
Two experiments were conducted to determine theeffect of handling, short-term storage, andinitial water stress on cutting water potential (W) and rooting of loblolly pine(Pinus taeda L.) stem cuttings. First,stock plants and cuttings were measured forW at predawn (04:00 a.m.) and earlymorning (09:00 a.m.). Cuttings were thensevered, wrapped in wet paper towels, andplaced in insulated containers for 2 or 7 h atapproximately 30 °C or for 21 h in coldstorage (4 °C). Water potentials ofcuttings were measured at the end of eachstorage period. Second, effects of initialwater stress on rooting performance of cuttingswere tested by withholding water from dormant(winter) and succulent (summer) cuttings forvarying periods of time. After each dryingtreatment, W was measured on asample of cuttings and the remainder of thecuttings were transferred to a greenhouse withintermittent mist for 12 weeks.Storage of cuttings for long periods (7 to 21h) of time under low vapor pressure deficitconditions resulted in less negative waterpotentials of the cuttings. Dormant cuttingsrooted at higher percentages, even after beingexposed to lower values of W Thelower values of W in dormantcuttings could be attributed to higher ambientvapor pressure deficit during the drying phase. Results suggest that subjecting cuttings tomoderate water stress for a short period oftime does not adversely affect the rooting ofcuttings. Cutting water potentials below –1.7MPa appeared to reduce rooting of succulentcuttings and water potentials below –2.0 MPaaffected rooting in dormant cuttings. 相似文献
27.
McIlwain DR Lang PA Maretzky T Hamada K Ohishi K Maney SK Berger T Murthy A Duncan G Xu HC Lang KS Häussinger D Wakeham A Itie-Youten A Khokha R Ohashi PS Blobel CP Mak TW 《Science (New York, N.Y.)》2012,335(6065):229-232
Innate immune responses are vital for pathogen defense but can result in septic shock when excessive. A key mediator of septic shock is tumor necrosis factor-α (TNFα), which is shed from the plasma membrane after cleavage by the TNFα convertase (TACE). We report that the rhomboid family member iRhom2 interacted with TACE and regulated TNFα shedding. iRhom2 was critical for TACE maturation and trafficking to the cell surface in hematopoietic cells. Gene-targeted iRhom2-deficient mice showed reduced serum TNFα in response to lipopolysaccharide (LPS) and could survive a lethal LPS dose. Furthermore, iRhom2-deficient mice failed to control the replication of Listeria monocytogenes. Our study has identified iRhom2 as a regulator of innate immunity that may be an important target for modulating sepsis and pathogen defense. 相似文献
28.
B W Calnek J C Carlisle J Fabricant K K Murthy K A Schat 《American journal of veterinary research》1979,40(4):541-548
An apparently nononcogenic Marek's disease virus (SB-1) and turkey herpesvirus could be readily isolated from spleen, bursa of Fabricius, thymus, and peripheral blood lymphocytes of chickens beginning 4 to 6 days after inoculation, but unlike infections with two isolates of oncogenic Marek's disease virus (JM-10 and CU-2), virus replication in these cells was rare, and necrosis in the organs was essentially absent. Splenic enlargement was observed regularly during the first 4 to 11 days after inoculation, and Marek's disease tumor-associated surface antigen was observed on splenic and other lymphocytes in the four viral inoculation groups. Cellular cytotoxicity of splenic lymphocytes was demonstrated in vitro with cultured Marek's disease tumor cells (MSB-1 lymphoblastoid cell line) as the target in a chromium-release assay. The four viral infections induced sensitized lymphocytes. 相似文献
29.
G.Devegowda T.N.K.Murthy 《中国家禽》2008,30(16)
在过去的几十年里,全球的禽蛋和禽肉产量在稳步地增加.虽然禽蛋和禽肉高的营养价值使得需求量不断提高,但是食品的质量和安全因素决定着禽类产品的市场价值,因此变得越来越重要.由于霉菌毒素是影响家禽生产力和产品质量的重要因素之一,因此控制霉菌毒素的污染非常关键. 相似文献
30.
Kadanthottu Sebastian Joseph Sushma Bolla Kavita Joshi Medha Bhat Krupa Naik Sridevi Patil Sreevani Bendre Brunda Gangappa Vidya Haibatti Shivanand Payamalle Smita Shinde Yaser Hassan Dewir Hosakatte Niranjana Murthy 《Erwerbs-Obstbau》2017,59(3):195-202
African mangosteen (Garcinia livingstonei T. Anderson) is native to Africa and is exotic to other parts of the world. It is a fruit bearing tree with multiple uses. This study was purposed to determine, by analysis, the chemical compositions and nutritive value of the fruits of African mangosteen. Proximate compositions varied with the portions such as epicarp, mesocarp, endocarp and seed tested. All fruit portions contained carbohydrate (37.67–95.02%) and crude protein (0.65–31.76%) as their major components. Moisture (0.45–3.42%), crude fat (1.23–19.55%), crude fiber (2.93–21.13%) and ash (1.76–5.44%) were also found at different levels, depending upon the portions. All portions of fruit were rich in macro and micro elements. The fruits also possessed phenolics (174.02–10.725?mg GAE per g), flavonoid (19.25 to 99.98?µg QE per g) and alkaloid (1.56 to 9.49?mg/kg) contents. The constitution of tannins and oxalate which contribute towards the anti-nutritive value was also low, thus making the fruits beneficial for consumption. Quality attributes like relative amount of oleic acid, total unsaturated fatty acid, acid value, free fatty acid, peroxide value and iodine value showed that the seed oil of African mangosteen can become one among the oleic acid based vegetable oils for consumption. 相似文献