Loss of reserves of Cu in liver when Cu supplements are withdrawn from dairy herds in the Waikato region     

AR Hittmann  ND Grace  SO Knowles 《New Zealand veterinary journal》2013,61(2):150-153

Abstract

AIM: To monitor the consequences of withdrawing mineral Cu supplements from two dairy herds with initially high concentrations of Cu in liver.

METHODS: Two herds were selected from dairy farms in the Waikato region of New Zealand that participated in an earlier survey of Cu supplementation practices and Cu status of dairy cows. The herds were fed pasture, grass and maize silage, plus palm kernel expeller (PKE) containing 25–30 mg Cu/kg dry matter (DM) fed at 2–4 kg/cow/day. No mineral Cu supplements were supplied from January 2009. Pasture samples were collected for mineral analysis in September 2008 and April 2009. Concentration of Cu in liver biopsies from the same 9–10 cows per herd was measured on three occasions between April 2009 and May 2010.

RESULTS: Pastures on both farms contained 10 mg Cu/kg DM, 0.1–0.5 mg Mo/kg DM and 3.5–4.0 g S/kg DM. The initial herd mean concentrations of Cu in liver were 1,500 (SD 590) and 1,250 (SD 640) μmol Cu/kg fresh tissue. In the absence of mineral Cu supplements, those mean concentrations decreased over 12 months to 705 (SD 370) and 1,120 (SD 560) μmol Cu/kg fresh tissue, respectively. For cows in the first herd, the rate of depletion of liver Cu reserves was influenced by initial concentration of Cu, such that high concentration led to faster loss according to first-order kinetics.

CONCLUSIONS: Mineral Cu supplementation was not necessary over 12 months for two dairy herds with mean concentrations of Cu in liver >1,250 μmol Cu/kg fresh tissue, grazing pastures containing 10 mg Cu/kg DM and concentrations of Mo <1 mg/kg DM. The quantity and particularly the duration of feeding PKE appeared to be a factor in whether or not the herd lost substantial reserves of Cu in liver during the year. However, the Cu status of both herds in this study was more than adequate to support late pregnancy and mating.

CLINICAL REVELANCE: Copper status of the herd should be monitored and on-farm management of Cu nutrition should take into account all sources contributing to daily intake of Cu. Where Cu supplementation has been excessive and there is risk of chronic Cu toxicity, mineral Cu supplements may be withdrawn for a period commensurate with the expected rate of liver Cu depletion.  相似文献   

Growth response to increasing doses of microencapsulated vitamin B12 and related changes in tissue vitamin B12 concentrations in cobalt-de cient lambs     

ND Grace  SO Knowles  GR Sinclair  J Lee 《New Zealand veterinary journal》2013,61(2):89-92

AIM: To investigate growth responses of cobalt-deficient lambs to increasing doses of microencapsulated vitamin B₁₂, and to measure associated changes in serum and liver vitamin B₁₂ concentrations over 243 days.

METHODS: From a flock grazing pastures that had low cobalt (Co) levels (about 0.06 mg Co/kg dry matter), 4-6-week-old lambs (n=137) were assigned to four groups and received either no treatment or a subcutaneous injection of 3.0, 4.5 or 6.0 mg of microencapsulated vitamin B₁₂ on Day 1. At approximately monthly intervals, all lambs were weighed and blood samples were collected from a selection (n=10) of monitor animals, up to Day 243. Liver biopsies were also carried out on the monitor lambs (n=8) on Days 1, 124 and 215.

RESULTS: The vitamin B₁₂-treated lambs grew significantly faster (p<0.001) than untreated animals. Liveweights after 243 days were 28, 45, 45 and 47 kg for the untreated, 3.0, 4.5 and 6.0 mg vitamin B₁₂-treated lambs, respectively. Of the initial group of untreated lambs, 68% had to be removed before the end of the trial because of substantial weight loss, but none of the treated animals were similarly afflicted. Serum vitamin B₁₂ concentrations increased in all vitamin B₁₂-treated lambs, reaching a peak at Day 25, and those of the 4.5 and 6.0 mg vitamin B₁₂-treated lambs remained significantly higher (except at Day 124) than the untreated lambs to Day 187. However, at Day 124, but not Day 215, the liver vitamin B₁₂ concentrations of treated lambs were two to three times higher than those of controls.

CONCLUSIONS: The growth rates of Co-deficient lambs were markedly improved by injection of 3.0, 4.5 or 6.0 mg of microencapsulated vitamin B₁₂, and liveweights were maintained for at least 243 days. Serum vitamin B₁₂ concentrations were related to this growth response; concentrations of <220 pmol vitamin B₁₂/l were associated with a 95% probability that lambs were Co-deficient and would thus respond to Co/vitamin B₁₂ supplementation. Based on these data, the current New Zealand reference criteria for Co deficiency should be reviewed.

CLINICAL SIGNIFICANCE: An injection of 3 mg microencapsulated vitamin B₁₂2 given to lambs at tailing will treat Co deficiency and will increase and maintain liveweights in a flock for up to 8 months.  相似文献   

A practical approach to managing the risks of iodine deficiency in flocks using thyroid-weight:birthweight ratios of lambs     

SO Knowles  ND Grace 《New Zealand veterinary journal》2013,61(6):314-318

AIM: To establish a protocol for determining the iodine status of a flock and managing the risks of iodine deficiency, using thyroid-weight:birthweight ratios of newborn lambs and serum iodine concentrations of ewes.

METHODS: Data were collected from iodine supplementation studies in Southland and Rangitikei, of sheep fed exclusively pasture or pasture plus brassica during the latter half of gestation (n=350, or 89 per treatment group). The ewes were supplemented pre-mating or at the time of pregnancy scanning with an injection of long-acting iodised oil. Serum iodine concentrations were measured in ewes prepartum in Rangitikei and postpartum at both locations. The thyroid-weight:birthweight ratios (as g/kg) in 229 newborn lambs were determined at post-mortem examination and compared between iodine supplemented vs unsupplemented flocks using probit analysis. Samples of pasture and kale were analysed monthly for determination of iodine and selenium.

RESULTS: Initial mean serum iodine concentration of all ewes was 41 µg iodine/L. Supplementation increased serum iodine concentrations regardless of forage fed, and concentrations remained high for between 127 and 206 days. The range of thyroid-weight:birthweight ratios in lambs from supplemented ewes was 0.09–0.70 (mean 0.35, standard deviation (SD) 0.147) g/kg. Among lambs from unsupplemented ewes, the range was much wider (0.21–8.5; mean 1.61, SD 1.95 g/kg). About half of those ratios were >0.8 g/kg and clearly indicative of goitre, including 62% from the groups on brassica and 18% from the Rangitikei group fed exclusively pasture. Probit calculations showed that a ratio of 0.40 (95% confidence interval (CI)=0.29–0.47) g/kg predicted with 35% probability, and a ratio of 0.80 (95% CI=0.70–0.99) g/kg predicted with 90% probability that a lamb came from an unsupplemented flock.

CONCLUSIONS: Compared to iodine concentrations in forages, thyroid-weight:birthweight ratios more accurately reflected the iodine status of the flock and could be used to identify which flocks to supplement the following year. Serum iodine concentrations of ewes measured before or after lambing did not reflect forage fed, but values near 40 µg/L were associated with goitrous lambs.

CLINICAL RELEVANCE: Thyroid-weight:birthweight ratios >0.8 g/kg were indicative of iodine deficiency, and ewes should be supplemented pre-mating or during pregnancy to prevent goitre the following year. Ratios <0.4 g/kg rarely occurred among deficient flocks, so the probability of benefit from supplementation was low. Intermediate ratios were ambiguous, and the iodine status of the flock could not be determined from biomarkers. In such cases individual-farm supplementation trials might be required to detect and manage the risks of marginal deficiency.  相似文献   

Cases of tuberculosis with no visible lesions     

THOMSON WM 《Journal of the American Veterinary Medical Association》1954,124(925):307-309

  相似文献   

The effect of drying and processing red clover on the digestion of the energy and nitrogen moieties in the alimentary tract of sheep     

D. E. BEEVER  D. J. THOMSON 《Grass and Forage Science》1981,36(3):211-219

The effect of artificial dehydration and processing of red clover on nutrient digestion and absorption has been studied with sheep. A total of three diets were fed, each at an intake of 900 g DM d^-1; comprising frozen (to represent fresh control), wafered and pelleted red clover.
Dehydration caused small depressions in energy and N digestibility. Grinding and pelleting had no further effect, although both treatments reduced the amount of energy digestion occurring in the rumen, frozen 63, dried 60, pelleted 48 MJ per 100 MJ digestible energy. Digestion of energy in the caecum and colon was increased on both dried diets, and in the small intestine on the pelleted diet.
Almost 40% of the extra duodenal energy on the pelleted diet was due to increased cellulose flow, with only 79% of digestible cellulose being digested in the rumen compared with 96% on the other two diets. There was an associated decrease in ruminal volatile fatty acid production of approx. 20%. Approximately half of the extra energy was due to an elevated flow of protein on the pelleted diet, but this increase was not reflected in amino acid absorption due to a reduced efficiency with the dried diets (frozen 72%, dried diets 54%).
The results demonstrate that dehydration and processing of red clover can markedly influence nutrient digestion and supply and thus offer attractive means of improving the nutritive value.  相似文献   

Evaluation of parameters obtained from two systems of gait analysis     

ND Assaf  SC Rahal  LR Mesquita  WT Kano  RB Abibe 《Australian veterinary journal》2019,97(10):414-417

  相似文献   

Diseases of eucalypts in the central and northern provinces of Mozambique     

Sílvia ND Maússe-Sitoe  ShuaiFei Chen  Michael J Wingfield 《Southern Forests》2016,78(3):169-183

In the mid-1970s, Mozambique embarked on several forestry projects to promote and establish large plantation areas with eucalypt trees. The planted species included Corymbia citriodora, Eucalyptus camaldulensis, E. saligna and E. tereticornis. Similar to other regions of the world, pests and pathogens pose a significant threat to eucalypt plantations in Mozambique, but little is known regarding the diseases of trees in the country. The aim of this study was to provide the first detailed consideration of the possible disease problems affecting plantation- grown eucalypt species in the central and northern provinces of Mozambique. Isolates of fungal pathogens were obtained from material displaying disease symptoms collected during two field surveys conducted in 2010 and 2011. Representative fungal isolates were identified using DNA sequence data. Stem canker diseases caused by fungi in the Botryosphaeriaceae, Cryphonectriaceae and Teratosphaeriaceae, as well as leaf spots caused by species of Calonectria, Pilidiella, Pilidium and Phakopsora myrtacearum were commonly encountered. Their relative importance to the future of planation forestry in Mozambique is discussed.  相似文献   

Toxicity to Diaphania hyalinata,selectivity to non‐target species and phytotoxicity of furanones and phthalide analogues          下载免费PDF全文

Gabriela C Resende  Elson S Alvarenga  Tamíris A de Araújo  Júlia ND Campos  Marcelo C Pincanço 《Pest management science》2016,72(9):1772-1777

  相似文献   

不同温度下大西洋鳕（Gadus morhua）个体发生早期胚胎生长和卵黄蛋白的利用     

Kuft.  ND 《福建水产》1998,(1):80-83

本文报道了自受精至孵化,不同培育温度（０－６．１℃）大西洋鳕胚胎生长和卵黄及卵蛋白吸收动态。  相似文献   

An economic evaluation of alternative genetic improvement strategies for farm woodland trees     

PALMER  H. E.; NEWTON  A. C.; DOYLE  C. J.; THOMSON  S.; STEWART  L. E. D. 《Forestry》1998,71(4):333-347

A cost-benefit analysis (CBA), focusing on the Net Present Value(NPV) of a current genetic improvement programme for broadleavedtrees was performed using Monte Carlo simulation, with an add-onsoftware package (‘@RISK’) specifically designedto take account of the uncertainty associated with long-termprojects. The CBA was undertaken by evaluating the total costof achieving a given estimated genetic gain via each of thebreeding strategies considered. The estimated values of geneticgain were then expressed in terms of the increased value oftimber output. Cash flows were based on current estimated treeestablishment costs and anticipated productivity of the fourtree species included in the programme (ash, Fraxinus excelsior;sycamore, Acer pseudoplatanus; wild cherry, Prunus avium; andsweet chestnut, Castanea sativa), when grown primarily for atimber crop. The results of the NPV analysis indicated thattree improvement could be cost-effective for small genetic gains,but that current breeding strategies differed markedly in theircost-effectiveness. Improvement scenarios based on conventionalselection and testing techniques, such as simple mass selectionand recurrent selection (seed orchards), were found to be themost cost-effective at a discount rate of 6 per cent. In contrast,tree improvement scenarios based on clonal techniques consistentlyranked lowest, despite the much higher genetic gains achieved.The use of clonal techniques was found to be particularly hardto justify with broadleaved tree species of relatively low timbervalue. Overall, with the current state of broadleaved timbermarkets in the UK, and the current areas being planted, investmentin basic genetic improvement of high-value timber species appearsfinancially worth while. The estimated direct additional financialbenefit to growers, if new planting is undertaken with improvedstock as opposed to unimproved stock, is estimated to rangefrom £38 ha^–1 with Simple Mass Selection to £100ha^–1 with Simple Recurrent Selection.  相似文献